Estimation of a new molecular marker of the genus Stathmopoda (Lepidoptera: Stathmopodidae): Comparing EF1a and COI sequences

김소라,이원훈,이승환 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.1

The genus Stathmopoda Herrich-Schäffer, 1853, one of the agriculture and forestry pest groups in the world, is usually a tiny-sized insect and exhibits subtle morphological differences which make problems on morphological- based identification. In this study, for identifying Stathmopoda species, we tried to find a newmolecularmarker by using one mitochondrial cytochrome c oxidase subunit I (COI) gene and one nuclear gene, elongation factor 1 alpha (EF1a). From testing 42 individuals of six Stathmopoda species, we observed the usefulness of the EF1a in species identification with intraspecific genetic variation (range 0–2.5%) and interspecific genetic divergence (range 5.2–10.9%). In addition, the Neighbor-joining and Automatic Barcode Gap Discovery analyses revealed one complex group consisting of S. commoda, S. sp. and S. neohexatyla were clearly distinguished in the EF1a region. Our results indicate the possibility of the EF1a gene as a new molecular marker that can improve the accuracy of Stathmopoda identification comparing with the COI regions.

Estimation of a new molecular marker of the genus Stathmopoda (Lepidoptera: Stathmopodidae): Comparing EF1a and COI sequences

Kim, S.,Lee, W.,Lee, S. 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol. No.

<P>The genus Stathmopoda Herrich-Schaffer, 1853, one of the agriculture and forestry pest groups in the world, is usually a tiny-sized insect and exhibits subtle morphological differences which make problems on morphological-based identification. In this study, for identifying Stathmopoda species, we tried to find a new molecular marker by using one mitochondrial cytochrome c oxidase subunit I (COI) gene and one nuclear gene, elongation factor 1 alpha (EF1a). From testing 42 individuals of six Stathmopoda species, we observed the usefulness of the EFl a in species identification with intraspecific genetic variation (range 0-2.5%) and interspecific genetic divergence (range 5.2-10.9%). In addition, the Neighbor-joining and Automatic Barcode Gap Discovery analyses revealed one complex group consisting of S. commoda, S. sp. and S. neohexatyla were clearly distinguished in the EF1a region. Our results indicate the possibility of the EFla gene as a new molecular marker that can improve the accuracy of Stathmopoda identification comparing with the COI regions. (C) 2016 Published by Elsevier B.V. on behalf of Korean Society of Applied Entomology, Taiwan Entomological Society and Malaysian Plant Protection Society.</P>

DNA barcoding of the stem borer, Stathmopoda spp. (Lepidoptera: Stathmopodidae)

Sora Kim,Wonhoon Lee,Yerim Lee,Seunghwan Lee 한국응용곤충학회 2013 한국응용곤충학회 학술대회논문집 Vol.2013 No.10

The mitochondrial COI gene has proven successful for identifying the lepidopteran species. In addition, this gene has been applied to recognize cryptic species and confirm a polyphagous species as one species. However, it has been also reported misidentification in the COI gene. As a result, some researchers have suggested the use of additional genes in species identification, especially, nuclear genes. In this study, we observed that a nuclear EF1agene is better than the mitochondrial COI gene in recognizing Stathmopoda species. For example, among the all species, 47 individuals of nine species, a large intraspecific pairwise difference were detected, up to 15.0% in universal barcoding regions of the COI gene, 22.0% in other part of the COI gene; however, 2.0% in EF1a. In contrast, Stathmopoda sp2 and S. commoda were separated into several clusters in each different COI regions, and some individuals of S. auriferella and S. commoda, were closer to each other separating from the cluster of the same species. But, we get clear results from the EF1a, Stathmopoda species well clustered and could be distinguished.

Molecular Identification of the Soybean Aphid on the Primary Host, Rhamnus davurica in Korea

Hyojoong Kim,Yeyeun Kim,Kim A. Hoelmer,Seunghwan Lee 한국응용곤충학회 2009 한국응용곤충학회 학술대회논문집 Vol.2009 No.05

The soybean aphid, Aphis glycines Matsumura 1917, is well known as a soybean pest in the world. Recently, it has been introduced to North America causing serious damage in U.S. As a cooperative research with USDA-ARS, we have investigated A. glycines in soybean fields, and also examined the colonies on the overwintering host Rhamnus davurica in order to find its natural enemies. It was generally reported that A. glycines has host alternation between the soybean, Glycine max (summer host) and the Dahurian buckthorn, Rhamnus davurica (winter host) in East Asia. However, it was very difficult to identify the soybean aphid, A. glycines, from R. davurica due to the co-existance of at least three Aphis species and the seasonal polymorphisms of each species (e.g, gynopara, ovipara, and male). For species identification, we tested 3 molecular markers, mitochondrial COI, COII, and nuclear EF1α, for 14 collected samples (7 samples from G. max and 7 samples from R. davurica). As a result, we found two different species, A. gossypii and other Aphis sp., are mixed together with A. glycines on R. davurica. We report the biology of A. glycines in Korea, and present species identification using molecular phylogenetic approach.

New Report of Three Unrecorded Species in Trichoderma harzianum Species Complex in Korea

( Seokyoon Jang ),( Sun Lul Kwon ),( Hanbyul Lee ),( Yeongseon Jang ),( Myung Soo Park ),( Young Woon Lim ),( Changmu Kim ),( Jae-jin Kim ) 한국균학회 2018 Mycobiology Vol.46 No.3

The genus Trichoderma (Hypocreaceae, Ascomycota) consists of globally distributed fungi. Among them, T. harzianum, one of the most commonly collected Trichoderma species, had been known as a polyphyletic or aggregate species. However, a total of 19 species were determined from the polyphyletic groups of T. harzianum. Thus, we explored Korean “T. harzianum” specimens that were collected in 2013-2014. These specimens were re-examined based on a recent study with translate elongation factor 1-alpha (EF1a) sequences to reveal cryptic Trichoderma species in Korea. As a result, four different species, T. afroharzianum, T. atrobruneum, T. pyramidale, and T. harzianum, were identified. Except T. harzianum, the other three species have not been reported in Korea. In this work, we describe these species and provide figures.

DNA barcoding identification and genetic diversity of bamboo shoot wireworms (Coleoptera: Elateridae) in South China

Shouke Zhang,Yaning Liu,Jinping Shu,Wei Zhang,Yabo Zhang,Haojie Wang 한국응용곤충학회 2019 Journal of Asia-Pacific Entomology Vol.22 No.1

Wireworms are recognized as economically important oligophagous pests of Phyllostachys bamboo shoot, which have caused a huge economic loss in South China. Studies of bamboo shoot wireworm control and management were seriously hampered by the lack of reliable identification of larvae. DNA barcoding has been proved to be an important and useful tool in species identification of morphologically cryptic insects. Accurate knowledge of damage-causing species and the phylogenic structure of elaterids will provide insight into their sustainable and effective management. Here, we use interspecific variation in COI and EF1-a as a robust method of identification and consider the intra- and interspecific genetic variation of bamboo wireworms. This approach has revealed that three Melanotus larvae occurred in Phyllostachys forest. The dominance of one species in different sampling location suggests there maybe underlying difference in habit and host plant preference. These data provide a starting point for determining the distribution of wireworm species feeding on bamboo shoots in South China. The inclusions would further aid identification of wireworm species and relationships between certain wireworm species with specific environments.

Diversity of Pleurotus nebrodensis strains and detection a specific molecule marker

Qi Tan,C. Peter Romaine,Carl Schlagnhaufer,Mingjie Chen,Qian Guo,Jozsef Geml 한국버섯학회 2004 한국버섯학회 국제학술대회 Vol.3 No.-