천식과 아토피피부염 환자에서 D. farinae 특이 IgE 결합 양상의 차이

김규리 ( Gyu Ri Kim ),김민환 ( Min Hwan Kim ),최수영 ( Soo Young Choi ),이용원 ( Yong Won Lee ),박중원 ( Jung Won Park ),홍천수 ( Chein Soo Hong ) 대한천식알레르기학회 2008 천식 및 알레르기 Vol.28 No.3

Background: Dermatophagoides farinae (D. farinae) has various isoallergens. However, allergenic differences between bronchial asthmatics (BA) and atopic dermatitis (AD) patients have not yet been fully evaluated. Objective: To investigate the differences in D. farinae specific IgE between BA and AD patients sensitized to D. farinae. Method: Five BA and five AD patients sensitized to D. farinae were measured in this study. D. farinae-specific IgE was evaluated by 1-dimensional (1-D) and 2-dimensional (2-D) SDS-PAGE, and IgE immunoblotting. N-terminal sequencing was performed for the identification of the allergens. Result: Two-D IgE immunoblot revealed the 7 allergens with 14 kDa at PI 5.5 to 6.5. These allergens were found in 4, BA and 2 AD patients. Another allergen with 25 kDa at pI 7.8 was found only in 4 BA patients. This allergen was regarded as triosephosphate isomerase by N-terminal sequencing. Conclusion: Seven allergens with 14 kDa at pI 5.5 to 6.5 and 1 allergen with 25 kDa at pI 7.8 were identified more frequently in BA than in AD patients. The 25 kDa allergen was regarded as triosephosphate isomerase, and it might be associated with the development of asthma. (Korean J Asthma Allergy Clin Immunol 2008;28:199-204)

NC/Nga 마우스에서 Dermatophagoides farinae의 피부 적용과 기도 유발에 의해 발생한 기도 염증에 대한 CpG Oligodeoxynucleotide의 효과

김영준 ( Young Joon Kim ),장성옥 ( Seong Ok Jang ),최원아 ( Won Ah Choi ),강미진 ( Mi Jin Kang ),정현돈 ( Hyun Don Jung ),김하정 ( Ha Jung Kim ),김형영 ( Hyung Young Kim ),서주희 ( Ju Hee Seo ),유진호 ( Jin Ho Yu ),홍수종 ( Soo Jo 대한천식알레르기학회 2011 천식 및 알레르기 Vol.31 No.2

Background: Atopic dermatitis (AD) is the initial step of allergic march that progresses to airway allergic disease. There has been much interest in the interruption of the allergic march, but the results are not promising. CpG oligodeoxynucleotide (ODN) has been reported to have both preventive and therapeutic effects on the development of asthma in mouse models. Objective: To identify the development of airway inflammation after having atopic dermatitis-like skin lesions induced by topical application of Dermatophagoides farinae (Df), and to evaluate the preventive effect of CpG ODN on the development of airway inflammation. Method: We injected 5 μg of Df intradermally 8 times for 2 weeks into 8-week-old NC/Nga mice and then challenged them with 3 times of Df intranasally. CpG ODN with or without Df was injected subcutaneously before airway challenge. Airway hyperreactivity was determined by the change inenhanced pause (Penh) during methacholine provocation. Lung tissue and bronchoalveolar lavage specimens were evaluated. Result: Topical Df application induced AD-like skin lesion microscopically, but not macroscopically. NC/Nga mice with topical Df application developed more severe airway inflammation upon intranasal Df challenge than those with topical PBS application. However, the mice with CpG ODN injection before airway challenge showed reduced airway inflammation. The levels of Df-specific IgG1 were increased in the mice with both topical Df application and intranasal Df challenge but were decreased in the mice with CpG ODN injection. Conclusion: These results suggest that CpG may inhibit the development of airway inflammation from those with previous AD-like skin lesions induced by Df. (Korean J Asthma Allergy Clin Immunol 2011;31:131-139)

아토피피부염 환자에서 집먼지진드기 항원 - 자가항체 면역 복합체 치료효과

박중원(Jung Won Park),홍천수(Chein Soo Hong),이광훈(Kwang Hoon Lee),최현주(Hyun Joo Choi),최유진(Yoo Jean Choi),김수민(Soo Min Kim) 대한천식알레르기학회 1999 천식 및 알레르기 Vol.19 No.6

Background: Exposure to airborne allergens exacerbates symptoms of atopic dermatitis in sen- sitive patients. Objective : We attempted to evaluate the therapeutic effect of Dermatophagoides farinae anti- gen-autoantibody immune complex therapy in atopic dermatitis. Methods : Four patients with atopic dermatitis were treated with antigen-autoantibody im- mune complex therapy using D. farinae antigen for 1 year. The therapeutic effect was evaluated by clinical severity (erythema, excoriations, scale, pruritus, lichenification) and D. farinae specif- ic antibodies, skin test to D. farinae, total IgE, eosinophil count and soluble vascular cell adhesi- on molecule-1 (VCAM-1). Results : One of four patients had significant improvement in clinical severity and decrease in serum eosinophil count, but three of four patients had no significant improvement in clinical se- verity, skin test to D. farinae, total IgE, specific antibodies and soluble VCAM-1 after allergen- antibody complex therapy except specific IgE to D. farinae. Conclusion : The efficacy of allergen-antibody complex therapy may be limited in some atopic dermatitis patients.

Evaluation of the acaricidal toxicities of camphor and its structural analogues against house dust mites by the impregnated fabric disc method

Jeon, Ju‐,Hyun,Yang, Ji‐,Yeon,Lee, Hoi‐,Seon John Wiley Sons, Ltd 2014 Pest Management Science Vol.70 No.7

<P><B>Abstract</B></P><P><B>BACKGROUND</B></P><P><B>The acaricidal activities of (±)‐camphor structural analogues against house dust mites were evaluated using the impregnated fabric disc bioassay.</B></P><P><B>RESULTS</B></P><P><B>The acaricidal effects of camphor and its structural analogues were evaluated against <I>Dermatophagoides farinae</I> and <I>D. pteronyssinus</I>. Based on the LD<SUB>50</SUB> values against <I>D. farinae</I>, (±)‐camphor (0.95 µg cm<SUP>−2</SUP>) was 38.75 times more effective than <I>N</I>,<I>N</I>‐diethyl‐<I>m</I>‐toluamide (DEET) (36.81 µg cm<SUP>−2</SUP>), followed by (+)‐camphor (1.41 µg cm<SUP>−2</SUP>), (−)‐camphor (2.03 µg cm<SUP>−2</SUP>) and (1R)‐camphor oxime (3.31 µg cm<SUP>−2</SUP>) in the impregnated fabric disc bioassay. However, camphor‐10‐sulfonic acid and camphoric acid had no observable activity against <I>D. farinae</I> or <I>D. pteronyssinus</I>. The acaricidal activities of camphor and its structural analogues against <I>D. pteronyssinus</I> were similar to those against <I>D. farinae</I>.</B></P><P><B>CONCLUSION</B></P><P><B>These results indicate that camphor and its structural analogues are suitable for producing acaricidal agents against house dust mites. © 2014 Society of Chemical Industry</B></P>

Acaricidal Activity of Cade Oil Constituents and Spray Formulations toward Dermatophagoides farinae (Acari: Pyroglyphidae)

Haribalan Perumalsamy,Karuppasamy Sankarapandian,Xue Wang,Hye Min Shin,Young Joon Ahn 한국응용곤충학회 2016 한국응용곤충학회 학술대회논문집 Vol.2016 No.10

The American house dust mite, Dermatophagoides farinae Hughes, 1961 (Acari: Pyroglyphidae), is recognized as an important source of allergens in the domestic environment. An assessment was made of the toxicity of constituents from essential oil of cade, Juniperus oxycedrus L. (Cupressaceae), and related compounds as well as four experimental spray formulations containing the oil (10–40 g/liter sprays) to adult D. farinae. In a contact + fumigant mortality bioassay, methyleugenol (24-h LC50,5.82 μg/cm2) and guaiacol (8.24 μg/cm2) were the most toxic constituents to the mites and the toxicity of these constituents and benzyl benzoate did not differ significantly.

Toxicity of basil oil constituents and related compounds and efficacy of spray formulations containing the oil to Dermatophagoides farinae

Haribalan Perumalsamy,Jun-Ran kim,JaeYeonKim,Kum Na Ra Hwang,Young-JoonAhn 한국응용곤충학회 2013 한국응용곤충학회 학술대회논문집 Vol.2013 No.10

An assessment was made of the toxicity of basil, Ocimum basilicum, essential oil, 11 basil oil constituents, seven structurally related compounds and another 22 previously known basil oil constituents and the control efficacy of four experimental spray formulations containing the oil (1, 2, 3 and 4% sprays) to adult American house dust mites, Dermatophagoides farinae. Results were compared with those of two conventional acaricides benzyl benzoate and N,N-diethyl-3- methylbenzamide (deet). The active principles were determined to be citral, α -terpineol and linalool. Citral (24 h LC50, 1.13 μg/cm2) and menthol (1.69 μg/cm2) were the most toxic compounds, followed by methyl eugenol (5.78 μg/cm2). The toxicity of these compounds was more toxic than that of benzylbenzoate(LC50, 8.41 μg/cm2) and deet (37.67 μg/cm2). These compounds were consistently more toxic in closed versus open containers, indicating that their mode of delivery was largely a result of vapour action. Basil applied as 3 and 4% experimental sprays provided 97 and 100% mortality against the mites respectively, whereas permethrin (cis:trans, 25:75) 2.5 g/l spray treatment resulted in 17% mortality. Reasonable mite control in indoor environments can be achieved by spray formulations containing the basil oil (3 and 4% sprays) as potential contact-action fumigants.

Acaricidal Activity of Juniperus oxycedrus Essential Oil Constituents and Efficacy of Spray Formulations Toward Adult Dermatophagoides farina

Haribalan Perumalsamy,Jun-Ran Kim,Young-Joon Ahn 한국응용곤충학회 2014 한국응용곤충학회 학술대회논문집 Vol.2014 No.04

The toxicity of cade oil (Juniperus oxycedrus), its constituents and structurally related compounds toward adult house dust mite Dermatophagoides farina was examined. Results were compared with two commercially available acaricides, benzyl benzoate and deet. The cade oil constituents were identified by GC-MS analysis. Citral (LC50, 1.13 μg cm–2) and methyleugenol (LC50, 5.78 μg cm–2) were the most toxic compounds, followed by eugenol (LC50,12.52 μg cm–2), nerol (LC50, 21.4 μg cm–2) and terpinen-4-ol (LC50, 29.55 μg cm–2) were shown significant mortality against adult of D. farina. Toxicity of citral, methyleugenol were higher than that of benzyl benzoate, and above listed compounds was all more toxic than deet. Given the result of vapour-phase mortality tests that these compounds were more toxic in closed containers than in open ones, we concluded that vapour action plays a great role in their mode of delivery. Cade applied as 3 and 4% experimental sprays provided 96 and 100% mortality against the mites respectively, whereas permethrin (cis:trans, 25:75) 2.5 g/l spray treatment resulted in 17% mortality. Cade oil, and its constituents shown their potentials as effective alternatives for harmful synthetic acaricides for the control of Dermatophagoides populations as fumigants in contact and therefore illustrated the need for further study of this essential oil.

Toxicity of hiba oil constituents and spray formulations to American house dust mites and copra mites

Kim, Jun‐,Ran,Perumalsamy, Haribalan,Kwon, Min Jung,Chae, Se Um,Ahn, Young‐,Joon John Wiley Sons, Ltd 2015 Pest Management Science Vol.71 No.5

<P><B>Abstract</B></P><P><B>BACKGROUND</B></P><P><I>Dermatophagoides farinae</I> and <I>Tyrophagus putrescentiae</I> are recognised as an important source of allergens. An assessment was made of the toxicity of hiba, <I>Thujopsis dolabrata</I> var. <I>hondai</I>, oil and 13 organic compounds and the control efficacy of four experimental spray formulations containing the oil (5–30 g L<SUP>−1</SUP> spray) against both mite species.</P><P><B>RESULTS</B></P><P>In a contact + fumigant mortality bioassay, (−)‐thujopsene was the most toxic constituent against <I>D. farinae</I> and <I>T. putrescentiae</I> (24 h LC<SUB>50</SUB> 9.82 and 10.92 µg cm<SUP>−2</SUP>), and the toxicity of the compound was nearly identical to that of benzyl benzoate (9.33 and 10.14 µg cm<SUP>−2</SUP>). The toxicity was more pronounced in carvacrol, (+)‐terpinen‐4‐ol, <I>β</I>‐thujaplicin, (−)‐terpinen‐4‐ol, cedrol and <I>α</I>‐terpineol (LC<SUB>50</SUB> 12.05–15.20 and 12.74–16.48 µg cm<SUP>−2</SUP>) than in <I>N</I>,<I>N</I>‐diethyl‐3‐methylbenzamide (LC<SUB>50</SUB> 35.53 and 38.42 µg cm<SUP>−2</SUP>) against both mite species. The hiba oil 30 g L<SUP>−1</SUP> spray and commercial permethrin (<I>cis</I>:<I>trans</I> 25:75) 2.5 g L<SUP>−1</SUP> spray treatment resulted in 100 and 11% mortality against the two mite species respectively. In vapour‐phase mortality tests, the two compounds were consistently more toxic in closed versus open containers, indicating that toxicity was achieved mainly through the action of vapour.</P><P><B>CONCLUSION</B></P><P>Reasonable mite control in indoor environments can be achieved by a spray formulation containing the 30 g L<SUP>−1</SUP> hiba oil as a potential contact‐action fumigant. © 2014 Society of Chemical Industry</P>

Reduction of the IgE-binding ability and maintenance of immunogenicity of gamma-irradiated <i>Dermatophagoides farinae</i>

Lee, Ju-Woon,Seo, Ji-Hyun,Kim, Jae-Hun,Lee, Soo-Young,Park, Joong-Won,Byun, Myung-Woo Pergamon 2007 Radiation physics and chemistry Vol.76 No.11-12

<P><B>Abstract</B></P><P>House dust mites, <I>Dermatophagoides farinae</I> (DF) and <I>Dermatophagoides pteronyssinus</I>, are major allergens in the most common indoor allergen and are important risk factor for asthma. The modified antigen has been studied to treat allergic disorder. This study was carried out to measure possibility of modified allergen using gamma irradiation to treat allergy such as asthma. DF solutions (2mg/ml) as target allergen were irradiated with Co-60 at 50 and 100kGy. Conformational alternation of irradiated DF was observed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Levels of anti-irradiated DF mouse IgGs (sub-isotypes) against intact DF were measured similar to that of anti-intact DF IgGs. The binding abilities of house dust mite-allergic patients’ IgE were reduced depending on radiation dose, and irradiation could inhibit the binding ability of patients’ IgE more than 40%. This study has shown that the binding ability of IgE was reduced by conformational alteration by irradiation and the irradiated DF had epitopes capable to induce immunogeniciy.</P>

New Sensitization to House Dust Mites in Cefteram-Induced Occupational Asthma: A Case Report

Jin, Hyun Jung,Kim, Jeong-Eun,Kim, Joo-Hee,Ye, Young-Min,Park, Hae-Sim The Korean Academy of Asthma, Allergy and Clinical 2011 Allergy, Asthma & Immunology Research Vol.3 No.2

<P>Occupational asthma (OA) can improve after cessation of exposure; however, some patients suffer from persistence or aggravation of their asthmatic symptoms. Here we report a case of a new sensitization to house dust mites during the follow-up period in a 37-year-old female patient with OA induced by cefteram pivoxil powder (cefteram powder). She was previously diagnosed with OA caused by inhalation of cefteram powder. Consequently, she left her job and had been well for 9 subsequent years. She began to experience aggravation of her rhinitis and asthmatic symptoms again several months prior to presentation. Her skin-prick test results had converted to strongly positive responses to two types of house dust mites. The serum levels of eosinophil cationic protein (ECP) and the total and specific immunoglobulin (Ig)E levels against the two types of house dust mites were elevated. An inhalation challenge test with <I>Dermatophagoides farinae</I> was performed, and significant bronchoconstriction (21.1% reduction in the forced expiratory volume in the first second) with asthma symptoms was observed at 10 minutes. To our knowledge, this is the first case demonstrating a new sensitization to house dust mites in a patient with OA caused by cefteram powder. Regular monitoring, including skin-prick tests and measurement of specific serum IgE/ECP levels, may help to screen potential cases.</P>