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발생중인 생쥐 치아 및 치주조직에서 치주인대-특이 단백질; PDLs22의 발현
박중원,박병기,김상목,김병옥,박주철,Park, Jung-Won,Park, Byung-Ki,Kim, Sang-Mok,Kim, Byung-Ock,Park, Joo-Cheol 대한치주과학회 2002 Journal of Periodontal & Implant Science Vol.32 No.1
The periodontal ligament(PDL) is a unique tissue that is crucial for tooth function. However, little is known of the molecular mechanisms controlling PDL function. PDL-specific protein;PDLs22 had been previously identified as a novel protein isolated from cultured human PDL fibroblasts using subtraction hybridization between human gingival fibroblasts and PDL fibroblasts. The aim of this study was to examine the expression pattern and tissue localization of PDLs22 protein in embryonic and various postnatal stages of developing mouse using immunohistochemical staining. Embryos (E18) and postnatal (P1, P4, P5, P15, P18) were decapitated and the heads were fixed overnight in a freshly prepared solution of 4% paraformaldehyde. Some specimens were decalcified for $2{\sim}4$ weeks in a solution containing 10% of the disodium salt of ethylenediamine-tetraacetic acid (EDTA). Next, tissues were dehydrated, embedded in paraffin and sectioned serially at $6{\mu}m$ in thickness. Polyclonal antiserum raised against PDLs22 peptides, ISNKYLVKRQSRD, were made. The localization of PDLs22 in tissues was detected by polyclonal antibody against PDLs22 by means of immunohistochemical staining. The results were as follows; 1. Expression of PDLs22 protein was not detected in the tooth germ of bud and cap stage. 2. At the late bell stage and root formation stage, strong expression of PDLs22 protein was observed in developing tooth follicle, osteoblast-like cells, and subodontoblastic cells in the tooth pulp, but not in gingival fibroblasts, ameloblasts and odontoblasts of tooth germ 3. In erupted tooth, PDLs22 protein was intensely expressed in PDL and osteoblast-like cells of alveolar bone, but not in gingival fibroblasts, mature osteocytes and adjacent salivary glands. 4. In the developing alveolar bone and mid-palatal suture, expression of PDLs22 protein was seen in undifferentiated mesenchymal cells and osteoblast-like cells of developing mid-palatal suture, but not in mature osteocytes and chondrocytes. These results suggest that PDLs22 protein may play an important role in the differentiation of undifferentiated mesenchymal cells in the bone marrow and PDL cells, which can differentiate into multiple cell types including osteoblasts, cementoblasts, and PDL fibroblasts. However, more researches should be performed to gain a better understanding of the exact function of PDLs22 protein which related to the PDL cell differentiation.
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국내 분리 토끼출혈병 바이러스(RHDVa)를 감염시킨 토끼 간장에서의 경시적인 초미세구조 변화와 apoptosis
박중원,전지은,박은정,김한,이명헌,황의경,김재훈,이중복,우계형,Park, Jung-Won,Chun, Ji-Eun,Bak, Eun-Jung,Kim, Han,Lee, Myeong-Heon,Hwang, Eui-Kyung,Kim, Jae-Hoon,Lee, Chung-Bok,Woo, Gye-Hyeong 대한수의학회 2013 대한수의학회지 Vol.53 No.1
In this study, to understand the pathogenesis of new rabbit hemorrhagic disease virus (RHDVa) serotype, we carried out to administrate RHDVa to rabbits, and to examine sequential electron microscopic changes and relationship between pathogenesis and apoptosis. TUNEL-positive cells began to be observed from 24 hours after inoculation (HAI) and the number of positive cells was slightly increased with the course of time. Whereas marked increase of positive cells was seen in the liver from the rabbits died acutely. Typical viral particles with cup-like projections and a diameter of 30~40 nm were detected in homogenized liver samples and tissues at 36 and 48, and 48 HAI, respectively. Ultrastructurally, glycogen deposition was observed from the first stage of hepatocellular degeneration by RHDVa infection and then, swelling and disruption of cristae of mitochondria by viral particles, swelling of smooth endoplasmic reticulum, vacuoles and vesicles were detected. Condensation, margination and fragmentation of chromatin were observed in degenerative hepatocytes at 36 and 48 HAI, indicating apoptotic bodies. These data offer that hepatocytic apoptosis by RHDV infection could be closely related with mitochondrial impairment in the hepatocytes.
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새로운 국내 분리 토끼출혈병바이러스(RHDVa)를 감염시킨 토끼에서의 경시적인 병리학적 변화와 조직 내 바이러스 항원 분포
박중원,전지은,양동군,박은정,김한,이명헌,황의경,이중복,우계형,Park, Jung-Won,Chun, Ji-Eun,Yang, Dong-Kun,Bak, Eun-Jung,Kim, Han,Lee, Myeong-Heon,Hwang, Eui-Kyung,Lee, Chung-Bok,Woo, Gye-Hyeong 대한수의학회 2012 대한수의학회지 Vol.52 No.2
Rabbit hemorrhagic disease is a highly acute and fatal viral disease caused by rabbit hemorrhagic disease virus (RHDV). Since first outbreak in Korea 1987, RHDV has been continually affected in the country, but the pattern of outbreak seem to be changed. In this study, to understand the pathogenesis of the new RHDVa serotype, we therefore carried out to inoculate RHDVa to rabbits, and to examine the sequential histopathologic changes and viral distribution. Macroscopically, various sized dark red or white spots or appearance were observed in the liver, lung, kidney uterus and ureter. In euhanized rabbits, significant pathologic findings such as infiltration of heterophils and mononuclear cells were observed at 24 hours after inoculation (HAI), and these were sequentially extended periportal to centrilobular area. However, in dead rabbits, severe hepatic degeneration and/or necrosis with relatively weak inflammatory responses were observed. RHDV antigens began to detect in liver, spleen, and lung from 12 HAI by PCR. Immunohistochemically, RHDV positive cells were seen in only liver from 24 HAI, and the degree of immunogen reactivity was stronger in dead rabbits than in euthanized ones. In conclusion, RHDVa caused the subacute or chronic infection accompanying low mortality and moderate to severe inflammatory reaction in rabbits, suggesting the possibility that RHD could become endemic.
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박중원,박국양,김웅한,Park, Jung-Won,Park, Guk-Yang,Kim, Ung-Han 대한흉부심장혈관외과학회 1997 Journal of Chest Surgery Vol.30 No.8
인공판막심내막염은 드물지만 합병증으로 판막주위가 감염으로 파괴되고 농양이 형성되는 경우가 있어 높 은 사망율을 가져온다. 그러나 적절한 항생제의 사용, 심장초음파 검사에 기초를 둔 조기 진단,수술시 효과적인 심근보호. 그리고 수술경험의 축적으로 인공판막심내막염에 있어서 보다좋은 장단기 결과를 얻을 수 있다. 35세 남자 환자가 94년 8월에 대동맥판막, 승모판막대치술과 함께 삼첨판륜성형술을 받은후 특별한 증상 없이 지내다가 갑자기 시작된 실어증, 양안구의 좌측편위, 고열로 입원하여 심장초음파 검사를 시행한 결과 인공판막 심내막염 소견을 보여 6주간의 항생제 치료후 수술을 시행하였다. 수술소견상 대동맥 판륜을 따라 증식물 소견과 판막 주위의 누출, 농양주머니가 형성되어 있었고 승모판막은 비교적 건강해 보였다. 수술은 감염된 조직의 전체적인 제거와 냉동저장된 대동맥 동종이식편을 이용하여 재대치 수술을 시행했다. 환자는 수술후 63일째 퇴원했으며 퇴원후 7개월 동안 외래 추적 관찰결과 특별한 이상소견 없이 지내고 있다.
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디자인·공예 융합 교육 프로그램 사례연구 -국민대학교 「팀팀Class」를 중심으로-
박중원,Park, Jung-won 한국디지털정책학회 2021 디지털융복합연구 Vol.19 No.8
융합이라는 시대의 흐름과 더불어 상상력과 창조성에 기반을 둔 디자인·공예의 본질적 속성의 효과적 결합을 위해서는 학문적, 방법적 측면에서 다양한 시도가 필요하다. 본 연구는 2020년 2학기에 실행된 국민대학교 팀팀Class의 디자인·공예 융합 교육 프로그램의 운영 사례를 분석하는 것이다. 이를 위해 기존 문헌연구를 통한 디자인·공예 융합 교육 관련 중요 항목을 5가지(대상, 방법, 운영, 공간, 소통)로 구분해 분석하였다. 이를 통해 디자인·공예 융합 교육 프로그램에 대한 경험과 문제점, 그리고 향후 디자인·공예 융합 교육 프로그램의 운영에 적용 가능한 해결방안 제시에 본 연구의 목적이 있다. 본 연구에서는 국민대학교 팀팀Class 디자인·공예 융합 교육 프로그램으로 사례연구 대상을 한정하였으나 향후 지속적인 연구를 통해 향후 다가올 코로나(COVID-19) 이후 시대의 새로운 환경에 적용 가능한 디자인·공예 융합 교육 프로그램을 위해 후속 연구가 진행되어야 할 것이다. The tendency of the current times require education to focus on convergence, and the same applies to the essence of ceramics and design base imagination and creativity. For effective integration, a wide range of experimentations is required both in terms of academic and methodic approaches. This study analyzes the [TeamTeam Class] curriculum, converging ceramics with design initiated in the second semester (autumn semester) of 2020. Through reference materials on ceramics and design convergence education, it was possible to classify the following 5 categories: Subject, Method, Management, space and communication. The aim of the study is to find resolutions to overcome existing issues and problems in search of more effective methods. Although this study is based on convergence education, [TeamTeam Class] at Kookmin University, I hope to extend it further to also consider education after COVID-19.
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경증 기관지 천식 환자에서 Methacholine 흡입에 따른 Spirometer와 Body Plethysmographic Parameter의 변화에 관한 연구
박중원 ( Park Jung Won ),홍천수 ( Hong Cheon Su ) 대한내과학회 1993 대한내과학회지 Vol.44 No.2
Background : Methacholine inhalation test was known to be a good method for assessing the nonspecific bronchial hyperresponsiveness(NSBH). And a decline in forced expiratory volume in 1 second(FEV1) of more than 20% indicates the presence of NSBH. But there are some difficulties in determination of NSBH objectively as the results of spirometry are influenced by ptients motivations. And there are same differences in the mechanisms of airway narrowing between natural bronchial asthma and methacholine induced bronchial spasm. Methods : We measured spirometric and body plethys-mographic parameters both before and after inhalation of methacholine in 30 cases of mild asthmatics. To better understand the mechanism of the methacholine induced bronchospam, spirmetric and plethysmogra-phic parameters of methacholine induced bronchospasm(MIBS, n=18) were compared with natural bronchial asthma(n=18). The MIBS and natural bronchial asthma groups were matched with same predict value of FEV1(65 vs 67%)Results : After the inhalation of methacholine, maximal expiratory flow volume at 50% of total lung capacity(Vmax50) was decreased more than 20% compared to baseline value in all 30 cases and the Vmax25 was decreased more than 20% in 27 of the 30 cases. Inhalation of methaholine produced an 175% increase increase in airway resistance(Raw)and 239% increase in specific airway resistance(SRaw), and all of the 30 cases had an increase in Raw of more than 35 percent. Methacholine inhalation produced a 33.5% increase in residual volume (RV), however the RV was decreased or remained unchange after inhalation of methacholine in 7 out of 30 cases. There were no differences in total lung capacity and effective residual volume between before and after inhalation of methacholine. Vital capacity and diffusing capacity of Co after challenge were significantly decreased 15.1% and 8.7% respectively. With comparison of MIBS(n=18) and natural bronchial asthma(n=18), SRaw was significantly higher in MIBS than natural bronchial asthma (380.±243.7 va 247.8±102.0% of pred.) and no significant difference was found in all spirometric and other plethysmographic parameters. Conclusion : For assessing the NSBH, Raw, Vmax50 and Vmax25 are useful and objective parameters. And even though there are some pathophysologic differences between MIBS and natural bronchial asthma, we could not find significant differences in most parmeters of spirometer and plethysmographic parameters except SRaw between MIBS and natural asthma.
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악성 식도 협착에서의 식도 확장술 및 내시경적 내인공관 삽입술
박중원(Jung Won Park),김진욱(Jin Wook Kim),류지곤(Ji Kon Ryu),고광철(Kwang Cheol Koh),한철주(Cheol Joo Han),김용태(Yong Tae Kim),유권(Kwon Yoo),정현채(Hyun Chae Jung),윤용범(Yong Bum Yoon),송인성(In Sung Song),최규완(Kyu Wan Choi),김 대한내과학회 1992 대한내과학회지 Vol.43 No.5
Background: The prognosis of esophageal malignancy is poor and symptomatic palliation is important for those patients with malignant dysphagia and tracheoesophageal fistula. Endoscopic endoprosthesis insertion has been widely u for the palliative purpose, and authors analyzed the effects and complications of endoscopic esophageal dilation and endoprosthesis insertion. Methods: Thirty patients with dysphagia or frequent aspiraton due to esophagogastric malignancy were managed with esphageal dilation and/or endoscopic endoprosthetic tube insertion for the purpose of symptomatic palliation. Twenty-one patients had esophageal cancer, six had gastric cancer, and three had lung cancer. Results: Dysphagia was relieved in 72% of the patients and aspiration due to fistula was improved in 40% of the patients. Complications were as follows; Perforation (17%), tube obstruction (21%), dislocation (14%), bleeding (3.3%). Perforations were managed with conservative medical measures. There was no immediate procedure-related mortality. Conclusion: Esophgeal dilation and/or endoscopic endoprosthetic tube insertion were effective measure for the palliation of esophageal symptoms due to esophagogastric malignancies.
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