The Relationship between the Expression of Melanoma Differentiation-Associated Gene-7/Interleukin-24 (MDA-7/IL-24) and Clinicopathological Features in Colorectal Adenocarcinomas

Boram Seo,Young Seob Hong,Youngmin,Mee Sook Roh 대한의생명과학회 2012 Biomedical Science Letters Vol.18 No.4

The melanoma differentiation-associated gene-7 (MDA-7) protein, also known as interleukin-24 (IL-24), is a novel candidate of tumor suppressor that can induce apoptosis experimentally in a variety of human malignant cells. However, there have been few studies about its role in colorectal cancer. We performed immunohistochemical detection of MDA-7/ IL-24 in 399 tissue samples from primary colorectal adenocarcinoma patients using a tissue microarray. Western blotting was then done to confirm the immunohistochemical observations. MDA-7/IL-24 immunoreactivity was observed in 116 (29.1%) of the 399 colorectal adenocarcinoma cases. Analysis of the MDA-7/IL-24 expression by Western blotting confirmed the immunohistochemical results. The tumors with a negative MDA-7/IL-24 expression more frequently showed poor differentiation (P=0004), lymph node metastasis (P=0.001), deep invasion (P=0.008) and high stage (P=0.001). A subset of colorectal adenocarcinoma revealed a decreased expression of MDA-7/IL-24, and this was associated with progressive pathologic features. These findings suggest that loss of MDA-7/IL-24 expression may play a role in tumor growth and progression of colorectal adenocarcinomas.

Clinicopathological Features of Indonesian Breast Cancers with Different Molecular Subtypes

Widodo, Irianiwati,Dwianingsih, Ery Kus,Triningsih, Ediati,Utoro, Totok,Soeripto, Soeripto Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.15

Background: Breast cancer is a heterogeneous disease with molecular subtypes that have biological distinctness and different behavior. They are classified into luminal A, luminal B, Her-2 and triple negative/basal-like molecular subtypes. Most of breast cancers reported in Indonesia are already large size, with high grade or late stage but the clinicopathological features of different molecular subtypes are still unclear. They need to be better clarified to determine proper treatment and prognosis. Aim: To elaborate the clinicopathological features of molecular subtypes of breast cancers in Indonesian women. Materials and Methods: A retrospective cross-sectional study of 84 paraffin-embedded tissues of breast cancer samples from Dr. Sardjito General Hospital in Central Java, Indonesia was performed. Expression of ER, PR, Her-2 and Ki-67 was analyzed to classify molecular subtypes of breast cancer by immunohistochemistry. The relation of clinicopathological features of breast cancers with molecular subtypes of luminal A, luminal B, Her-2 and triple negative/basal-like were analyzed using Pearson's Chi-Square test. A p-value of <0.05 was considered statistically significant. Results: Case frequency of luminal A, Luminal B, Her-2+ and triple negative/basal-like subtypes were 38.1%, 16.7%, 20.2% and 25%, respectively. Significant difference was found in breast cancer molecular subtypes in regard to age, histological grade, lymph node status and staging. However it showed insignificant result in regard to tumor size. Luminal A subtype of breast cancer was commonly found in >50 years old women (p:0.028), low grade cancer (p:0.09), negative lymph node metastasis (p:0.034) and stage III (p:0.017). Eventhough the difference was insignificant, luminal A subtype breast cancer was mostly found in small size breast cancer (p:0.129). Her-2+ subtype breast cancer was more commonly diagnosed with large size, positive lymph node metastasis and poor grade. Triple negative/basal-like cancer was mostly diagnosed among <50 years old women. Conclusions: This study suggests that immunohistochemistry-based subtyping is essential to classify breast carcinoma into subtypes that vary in clinicopathological features, implying different therapeutic options and prognosis for each subtype.

BRAF-Mutated Colorectal Cancer Exhibits Distinct Clinicopathological Features from Wild-Type BRAF-Expressing Cancer Independent of the Microsatellite Instability Status

장민혜,김세훈,황대용,김욱연,임소덕,김완섭,황태숙,한혜승 대한의학회 2017 Journal of Korean medical science Vol.32 No.1

In patients with colorectal cancer (CRC), the BRAF V600E mutation has been reported to be associated with several clinicopathological features and poor survival. However, the prognostic implications of BRAF V600E mutation and the associated clinicopathological characteristics in CRCs remain controversial. Therefore, we reviewed various clinicopathological features, including BRAF status, in 349 primary CRCs and analyzed the relationship between BRAF status and various clinicopathological factors, including overall survival. Similar to previous studies conducted in Eastern countries, the incidence of the BRAF V600E mutation in the current study was relatively low (5.7%). BRAF-mutated CRC exhibits distinct clinicopathological features from wild-type BRAF-expressing cancer independent of the microsatellite instability (MSI) status. This mutation was significantly associated with a proximal tumor location (P = 0.002); mucinous, signet ring cell, and serrated tumor components (P < 0.001, P = 0.003, and P = 0.008, respectively); lymphovascular invasion (P = 0.004); a peritumoral lymphoid reaction (P = 0.009); tumor budding (P = 0.046); and peritoneal seeding (P = 0.012). In conclusion, the incidence of the BRAF V600E mutation was relatively low in this study. BRAF-mutated CRCs exhibited some clinicopathological features which were also frequently observed in MSI-H CRCs, such as a proximal location; mucinous, signet ring cell, and serrated components; and marked peritumoral lymphoid reactions.

Evaluation of Insulin Like Growth Facror-1 Genetic Polymorphism with Gastric Cancer Susceptibility and Clinicopathological Features

Farahani, Roya Kishani,Azimzadeh, Pedram,Rostami, Elham,Malekpour, Habib,Aghdae, Hamid Asadzadeh,Pourhoseingholi, Mohamad Amin,Mojarad, Ehsan Nazemalhosseini,Zali, Mohammad Reza Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.10

Gastric cancer (GC) is one of the most common malignancies in the world. It is the first cause of cancer deaths in both sexes In Iranian population. Circulating insulin-like growth factor-one (IGF-1) levels have been associated for gastric cancer. IGF-1 protein has central roles involved in the regulation of epithelial cell growth, proliferation, transformation, apoptosis and metastasis. Single nucleotide polymorphism in IGF-1 regulatory elements may lead to alter in IGF-1expression level and GC susceptibility. The aim of this study was to investigate the influence of IGF-1 gene polymorphism (rs5742612) on risk of GC and clinicopathological features for the first time in Iranian population. In total, 241 subjects including 100 patients with GC and 141 healthy controls were recruited in our study. Genotypes were analyzed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay with DNA from peripheral blood. The polymorphism was statistically analyzed to investigate the relationship with the risk of GC and clinicopathological properties. Logistic regression analysis revealed that there was no significant association between rs5742612 and the risk of GC. In addition, no significant association between genotypes and clinicopathological features was observed (p value>0.05). The frequencies of the CC, CT, and TT genotypes were 97%, 3%, and 0%, respectively, among the cases, and 97.9%, 2.1%, and 0%, respectively, among the controls. CC genotype was more frequent in cases and controls. The frequencies of C and T alleles were 98.9% and 1.1% in controls and 98.5% and 1.5% in patient respectively. Our results provide the first evidence that this variant is rare in Iranian population and it may not be a powerful genetic predisposing biomarker for prediction GC clinicopathological features in an Iranian population.

B형 간염 바이러스 감염과 연관된 사구체신염의 임상병리학적 특성과 신 조직내에서 HBs Ag 과 HBe Ag의 발현

정수진,김영주,윤혜경,정우영,김영훈,김수영,Jung Soo-Jin,Kim Young-Joo,Yoon Hye-Kyoung,Chung Woo-Yeong,Kim Young-Hoon,Kim Su-Yung 대한소아신장학회 1998 Childhood kidney diseases Vol.2 No.1

목적 : HBV 감염과 연관된 신병증의임상 및 검사소견과 병리학적 소견을 분석하여 그 특징을 규명하고, HBeAg과 HBsAg에 대한 면역조직화학염색을 신생검조직에서 시행하여 이들 항원의 사구체내에서의 면역학적 발현을 관찰하므로써 이 질환의 발생 기전에 대한 이해를 돕고자하였다. 방법 : 1990년 4월부터 1997년 2월까지 사구체신염으로 신조직 생검을 시행하여 부산백병원 해부병리과에 병리조직검사가 의뢰된 증례 중 혈청 HBsAg 양성인 28례를 대상으로 임상 및 검사소견을 조사하였고, 신조직의 광학현미경적, 면역형광학적, 전자현미경적 검사와 HBsAg과 HBeAg 항원에 대한 면역조직화학검사를 시행하였다. 결과 : 1. 연령 분포는 6세에서 73세였으며 소아 환자가 8명이었고 성인 환자는 20명 이었다. 평균 연령은 28세였고, 남녀 비는 3:1 이었다. 내원당시 67.9%에서 혈뇨, 75.0%에서 단백뇨를 보였고 57.2%에서 신증후군을 나타내었다. 전 예에서 혈청 HBsAg이 양성이었고, 혈청 HBeAg은 검사가 시행된 9례 중 6례 (66.6%)에서 양성이었다. 2. 간기능검사가 시행된 11례 중 7례 (63.6%)에서 AST와 ALT치가 증가되어 있었으며, 이중 간생검이 시행된 3례 모두 만성 활동성 간염으로 진단되었다. 3. 28례 중 막성 사구체 신염 (membranous glomerulonephritis: MGN)이 10례 (35.7%)로 가장 많았으며, 메산지움중식성 사구체신염 (mesangioproliferative glomerulonephritis: MesGN)이 8례 (28.6%), 막증식성 사구체 신염 (membranoproliferaive glomerulonephritis : MPGN)은 7례 (25.0%) 그리고 미세변화질환 (minimal change disease : MCD)가 3nl (10.7%)순이었다. 4. HBV와 연관된 MGN에서는 혈관간 세포의 증식 및 혈관간 기질의 확장이 자주 관찰되었으며, 사구체간질 내와 내피하 침윤도 동반되어 MPGN을 시사하는 소견이 함께 관찰되는 예가 많았고, HBV 항원 혈증에 동반된 MPGN에서는 내피하 침윤 뿐 아니라 기저막 내 및 상피세포하 침윤도 종종 관찰되었다. 5. 신조직의 면역조직화학검사상 HBsAg은 모두 음성이었으나 HBeAg은 28례 중 8례 (28.6%)에서 사구체 모세혈관벽을 따라 양성 반응을 나타내었고, 이 중 3례는 혈치 HBeAg 검사를 받았는데 모두 양성이었다. 결론 : HBV와 연관된 사구체신염은 다양한 병리학적 양상을 보여주며, 특히 MGN과 MPGN에서는 전자현미경소견상 두 질환의 특징이 중복되어 나타났고, 사구체내에서 HBeAg이 검출되어 HBV의 활동성 정도와 사구체신염의 발생이 연관성이 있을 것으로 사료되나 더 많은 연구보고가 있어야 하겠다. Purpose : Hepatitis B virus (HBV) infection has been involved in several forms of immune-related glomerulopathy but the pathogenic role of HBV infection is not clear. To evaluate the clinicopathological features of HBV-associated glomerulopathy, a clinicopathological analysis and immunohistochemical stain for HBs Ag and HBe Ag were done. Methods : Clinicopathological features of HBV-associated glomerulopathy were analyzed with renal biopsies in 28 HBsAg seropositive patients from April 1990 to February 1997 at Pusan Paik Hospital, and immunohistochemical evaluation for HBsAg and HBeAg was done in renal tissues. Light microscopic, immunofluorescent and electron microscopic examination and immunohistochemical staining for HBsAg (DAKO) and HBeAg (BIONIKE) of renal tissue were performed. Result ; 1. The age distribution was 6 to 73 years old, and eight were children and 20 were adults. Male : female ratio was 3:1. Nineteen (67.9%) and 21 (75.0%) of 28 cases showed hematuria and proteinuria, respectively at the time of biopsy. Sixteen (57.2%) of them had nephrotic syndrome. 2. Liver function test was performed in 11 patients and seven (63.6%) of them showed increased AST and ALT levels. Liver biopsy was done in three patients and revealed findings of chronic active hepatitis. 3. HBV-associated glomerulopathy was membranous glomerulonephritis (MGN) in 10 (35.7%), mesangiopathy in 8 (28.6%), membranoproliferative glomerulonephritis (MPGN) in 7 (25.0%) and minimal change disease in 3 (10.7%) out of 28 cases. 4. Ultrastructurally HBV-associated MGN showed conspicuous subepithelial deposits with intramembranous, mesangial and subendothelial deposits and proliferation of mesangial cells and matrix, which were suggestive of MPGN. In HBV-associated MPGN, intramembranous and subepithelial deposits were scattered. 5. Immunohistochemical staining revealed no expression for HBsAg, but positive reaction for HBeAg along capillary wall in 8 cases (28.6%), of which 3 cases were checked for serum HBeAg, all showed positivity. Conclusion : HBV-associated glomerulopathy showed a wide morphologic spectrum and overlapping ultrastructural features in MGN and MPGN, and the activity of hepatitis B virus may be related to the development of HBV-associated glomerulopathy but further studies are recommended to confirm this relationship.

Clinicopathologic Features and Molecular Subtypes of Breast Cancer in Young Women (Age ≤35)

Goksu, Sema Sezgin,Tastekin, Didem,Arslan, Deniz,Gunduz, Seyda,Tatli, Ali Murat,Unal, Dilek,Salim, Derya,Guler, Tunc,Coskun, Hasan Senol Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.16

Introduction: Breast cancer in young women is a relatively rare disease; however it tends to be more aggressive and is the leading cause of cancer death in this population. The aim of this study is to investigate the clinical and biological features of breast cancer arising in young Turkish breast cancer patients. Materials and Methods: Patients with breast cancer aged 35 or less (${\leq}35$ years) were selected for the study. In total 211 cases were included. Pathologic features; histologic subtypes, grade, lymphovascular invasion, axillary involvement, and stage were recorded for each. Results: The most common subtype was luminal B (36.5%), followed by luminal A (30.8%), triple negative (23.2%) and HER2+(9.5%) subtypes. Twelve percent of the patients had stage 4, 32.7% had stage 3, 46.4% had stage 2, and 6.2% had stage 1 disease at the time of diagnosis. Mean tumour diameter was 3.87 cm (range 0.3-13 cm). The axillary lymph nodes were positive in 74.4% of the patients, while lympho-vascular invasion was seen in 56.4%. Some 9.5% of patients had grade 1, 51.2% had grade 2, and 31.8% had grade 3 tumors. Conclusions: Young women with breast cancer in Turkey are more likely to present with luminal B subtype. Tumors in young women are more likely to present with advanced disease, to be high grade and and to have more lymphovascular invasion. Further research should focus on whether we need new treatment strategies for young patients with breast carcinoma.

Clinicopathological Features and Survival of Patients with Gastric Cancer with a Family History: a Large Analysis of 2,736 Patients with Gastric Cancer

정오,정미란,박영규,류성엽 대한위암학회 2017 Journal of gastric cancer Vol.17 No.2

The clinicopathological features and survival of the patients with gastric cancer with a FHX did not significantly differ from those of the patients without a FHX.

The impact of hutchinson's nail sign on T-stage in subungual melanoma

( Chihyeon Sohng ),( Jun Hong Park ),( Man Hoon Han ),( Yong Hyun Jang ),( Weon Ju Lee ),( Seok-jong Lee ),( Jun Young Kim ) 대한피부과학회 2018 대한피부과학회 학술발표대회집 Vol.70 No.2

Background: Hutchinson's nail sign (HS) is an important clinical clue to diagnose subungual melanoma (SUM). However, there are rare studies about other clinical importance of HS in SUM. Objectives: This study is to investigate the relationship between HS and clinicopathological features of SUM. Methods: A retrospective study was conducted with 61 SUM patients from 2006 to 2017. We reviewed medical records and photographs of the patients. Results: HS was seen in 46 (75.4%) of 61 SUM. The patients with HS showed ulceration more frequently (45.7%) and received sentinel lymph node biopsy (SLNB) more often (58.7%) than those without HS (13.3%, 26.7%). This tendency was also shown when HS exists on lateral nail folds (66.7% vs. 30.6% for ulceration, 83.3% vs. 42.9% for SLNB). The patients with HS on proximal nail fold or hyponychium showed nail changes more commonly (87.5%, 90.3%) than those without HS on those sites (62.1%, 60.0%). The patients with HS on 3 or 4 nail folds around the nail showed more frequent ulceration (66.7% vs. 30.6%), thicker Breslow thickness (2.52±3.03 mm vs. 1.46±2.80 mm), higher T-stage (≥2b, 66.7% vs. 30.6%) and higher stage (≥IIA, 66.7% vs. 30.6%) than those with HS on less than 3 nail folds around the nail. Conclusion: HS correlated with some clinicopathological features of SUM. Especially, when the HS exists on 3 or 4 nail folds around the nail, we should consider that T-stage of SUM might be higher than expected.

Clinicopathological Features and Survival of Patients with Gastric Cancer with a Family History: a Large Analysis of 2,736 Patients with Gastric Cancer

Jeong, Oh,Jung, Mi Ran,Park, Young Kyu,Ryu, Seong Yeob The Korean Gastric Cancer Association 2017 Journal of gastric cancer Vol.17 No.2

Purpose: Previous studies indicated conflicting results regarding the prognosis of gastric cancer with a family history (FHX). This study aimed to determine the clinicopathological features and survival of patients with gastric cancer with a FHX. Materials and Methods: We reviewed 2,736 patients with gastric cancer who underwent surgery between 2003 and 2009. The prognostic value of a FHX was determined in the multivariate model after adjusting for variables in the Asian and internationally validated prognostic models. Results: Of the patients, 413 (15.1%) had a FHX of gastric cancer. The patients with a FHX were younger (58.1 vs. 60.4 years; P<0.001) than the patients without a FHX. There were no significant differences in the histopathological characteristics between the 2 groups. A FHX was associated with a better overall survival (OS) rate only in the stage I group (5-year survival rate, 95% vs. 92%; P=0.006). However, the disease-specific survival (DSS) rate was not significantly different between the 2 groups in all stages. The multivariate model adjusted for the variables in the Asian and internationally validated prognostic models revealed that FHX has no significant prognostic value for OS and DSS. Conclusions: The clinicopathological features and survival of the patients with gastric cancer with a FHX did not significantly differ from those of the patients without a FHX.

P157 : Clinicopathological features and prognostic factors of cutaneous malignant melanoma for recent 7 Years (2007-2013)

( Kyou Chae Lee ),( Soo Yuhl Chae ),( Hyun Wuk Cha ),( Yong Hyun Jang ),( Weon Ju Lee ),( Do Won Kim ),( Seok Jong Lee ) 대한피부과학회 2014 대한피부과학회 학술발표대회집 Vol.66 No.2

Background: Studies for prognostic factor of malignant melanoma (MM) were well performed in the west due to relatively high incidence rate. However, there are little data of MM, available in Korea. Objectives: To investigate the current status of clinicopathological features and prognostic predictors of MM. Methods: One hundred sixty patients diagnosed with MM at our hospital from 2007 to 2013 were enrolled. We analyzed age, sex, tumor location, duration, thickness, ulceration, tumor-infiltrating lymphocytes (TIL), microscopic satellite (Ms), angiolymphatic invasion, mitosis, clinicopathologic subtype, and clinical stage by review of medical records and histopathological slides. Univariate analysis and multivariate analyses for survival were performed by means of the Cox proportional hazard model. Results: The ratio of female to male was 1.22 to 1 and the mean age was 60.6 years. Most of the tumors were located on the hands and feet. Acral lentiginous melanoma was the most common (69.6%), followed by nodular melanoma (12.8%), superficial spreading melanoma (12.8%), and lentigo maligna melanoma/lentigo maligna (4.7%). The poor prognosis was related to sex, Ms, mitosis, and stage by univariate analysis, whereas it was related to sex and Ms by multivariate analysis. Conclusion: Our study showed the clinicopathological features and prognostic factor of MM in Korea. But further study is needed because our study has limitation result from loss to follow-up in confirmed death.