홍화자 extract가 난소적출로 유발된 흰쥐의 골에 미치는 영향에 대한 형태계측학적 연구

박석우 ( Seok Woo Park ),박영회 ( Young Hoi Park ),금동호 ( Dong Ho Keum ) 한방재활의학과학회 2003 한방재활의학과학회지 Vol.13 No.1

Object : This study was carried out to investigate histomorphologic effect of Carthami Semen Extract On Osteoporosis In Ovariectomized Rats Methods : We used 15 female Sprague-Dawley rats. They were divided three groups, control and sham operated, Carthami Semen. We stained the specimens with Goldner,s modified Masson,s Trichrome. And then we measured bone histomorphometry using bioquant computer program of image-analysis system. The measurements included static parameter and dynamic parameter. We measured the thickness of osteoid and callus, and bone volume as static parameter, and mineral apposition rate as dynamic parameter. Results and Conclusions : 1. In the change of Ca, P there was no significant change between Carthami Semen and control group. 2. The Osteoid thickness and bone volume was increased in Carthami Semen group compared with control group. 3. The Cortical thickness and MAR was not significantly changed in Carthami Semen group compared with control group. From above result, it appears that Carthami Semen have effective on osteoporosis.

홍화자·녹용·자하거 약침이 골다공증에 미치는 영향

육태한,이창현,이학인,Yook, Tae-Han,Lee, Chang-Hyun,Lee, Hak-In 대한침구의학회 2001 대한침구의학회지 Vol.18 No.1

Objectives : This study was designed to evaluate on the effects of the Carthami semen Cervi pomtotrichum cornu Hominis placenta aquacupuncture on the osteoporosis in the rats Materials and Methods : In order to induce estrogen deficient osteoporosis, ovariectomy was done on rats. Then aquacupunctures were injected into the pai shu points corresponding to urinary bladder meridian of human body in experimental groups. And such indexes were measured as the change of body weight, bone mineral density, histological change of tibia, and levels of osteocalcin, bone alkaline phosphatase, calcium, phosphorus in serum. Results : 1. Significant changes of trabecular area % in epiphysis of tibia were shown between control group and experimental group II and between experimental group I and group II. 2. Significant changes of trabecular area % in diaphysis of tibia were shown between control and experimental groups. 3. Significant changes of serum osteocalcin were shown between control group and experimenta group I . 4. Significant changes of levels of serum bone alkaline phosphatase were shown between control group and experimental group I and II. Conclusions : The Carthami semen Cervi pomototrichum cornu Hominis placenta aquacupuncture is effective to estrogen deficient osteoporosis, ovariectomy was done on rats.

만성매일두통 환자에 대한 홍화 약침 치료 : 예비연구

김민경,박성욱,정우상,문상관,박정미,고창남,조기호,김영석,배형섭,Kim, Min-Kyung,Park, Seong-Uk,Jung, Woo-Sang,Moon, Sang-Kwan,Park, Jung-Mi,Ko, Chang-Nam,Cho, Ki-Ho,Kim, Young-Suk,Bae, Hyung-Sup 대한약침학회 2010 Journal of pharmacopuncture Vol.13 No.4

Objectives : This pilot study is designed to reveal the effect of Carthami-semen acupuncture treatment on chronic daily headache. Methods : 1. All the voluntary subjects were diagnosed as CDH. 2. Carthami-semen acupuncture is applied 2 times a week for 4 weeks. 3. The patients were assessed by Headache impairment test score(HIT), Short Form 36 (SF-36) Health Survey, and Headache Free Days(HFD) before and after 4 weeks treatment, and two weeks the treatment finished. Results : The result were as follows ; 1. HIT score decreased from $65.3{\pm}5.5$ to $53.1{\pm}9.4$, and furthermore declined to $49.5{\pm}8.0$ after 2 weeks the treatment finished. 2. HFD increased from 19.1% to 31.5% during the treatment time. Moreover it showed continuous effectiveness after 2 weeks the treatment finished. 3. SF-36 showed significant improvement on all of the QOL items. Conclusions : This pilot study suggests that Carthami-semen acupuncture treatment has an effect on the CDH patients.

홍화자(紅花子) 약침(藥針)의 병행치료가 요통(腰痛)치료에 미치는 효과

송희옥 ( Hee Ok Song ),김성수 ( Sung Soo Kim ),이종수 ( Jong Soo Lee ) 한방재활의학과학회 2003 한방재활의학과학회지 Vol.13 No.3

Abstract: Clinical Study on Effect of Carthami-Flos Herbal acupuncture theraphy on Low Back Pain Background and Purpose. Although Carthami-Flos Herbal acupuncture has been used for inflammatory musculoskeletal disorders such as osteorarthritis and rheumatoid arthritis of knee joint,but there are poor reports about uses for low back pain. So this study is designed to investigate the effects of Carthami-Flos Herbal acupuncture theraphy on low back pain. Subjects: 38 outpaients with low back pain were divided into two groups:20 samples and 18 controls. Methods: The control group took conventional acupuncture therapies daily while the sample group took the same treatments plus Carthami-Flos Herbal acupuncture as well. Outcome Measures: Visual Analogue Scale(VAS) score for pain (total 7 times), Oswestry Disability Index(ODI) for disability and range ofmovement(extention and flexion) were checked for assessments. Result: The sample group was significantly more effective in decreasing VAS score after 4th and 5th assessment than the control group(p<0.05). The duration of pain was not significantly. Patients with sciatica showed significantly better results than those without sciatica after first, second, and 4th assessment. ROM and ODI were not significant statistically. Conclusion: In this clinical study, Carthami-Flos Herbal acupuncture was effective in relieving low back pain, but not in improving activity of daily life and range of movement.

Single Intravenous-dose Toxicity of Water-soluble Carthami-flos Pharmacopuncture (WCF) in Rats

정다정,육태한,최유민,김석희,김종욱 대한약침학회 2014 Journal of pharmacopuncture Vol.17 No.3

Objectives: This study was performed to analyze the toxicity and to find the lethal dose of the test substance Water-soluble Carthami-flos pharmacopuncture (WCF) when used as a single intravenous-dose in 6-week-old, male and female Sprague-Dawley rats. Methods: The experiment was conducted at Biotoxtech according to Good Laboratory Practices. 20 female and 20 male Spague-Dawley rats were divided into 4 groups of 5 female and 5 male animals per group. The rats in the three experimental groups received single intravenous injections with 0.125-mL, 0.25-mL and 0.5-mL/animal doses of WCF, Groups 2, 3, and 4, respectively, and the control group, Group 1, received a single intravenous injection with a 0.5-mL dose of normal saline. Clinical signs were observed and body weight measurements were carried out for 14 days following the injections. At the end of the observation period, hematology, clinical chemistry, histopathological tests and necropsy were performed on the injected parts. Results: No deaths occurred in any of the groups. Also, no significant changes in body weight, hematological parameters or clinical chemistry test results between the control group and the experimental groups were observed. Visual inspection after necropsy showed no abnormalities. Histopathological tests on the injected parts showed no significant differences, except for Group 1 females; however, the result was spontaneous generation and had no toxicological meaning because it was not dose-dependent. Therefore, this study showed that WCF had no effect on the injected parts in terms of clinical signs, body weight, hematology, clinical chemistry, and necropsy. Conclusion: As a result of single intravenous-dose tests of the test substance WCF in 4 groups of rats, the lethal dose for both males and females exceeded 0.5 mL/animal. Therefore, WCF is a relatively safe pharmacopuncture that can be used for treatment, but further studies should be performed.

Single Intravenous-dose Toxicity of Water-soluble Carthami-flos Pharmacopuncture (WCF) in Rats

Jung, Da-Jung,Choi, Yoo-Min,Kim, Seok-Hee,Kim, Jong-Uk,Yook, Tae-Han KOREAN PHARMACOPUNCTURE INSTITUTE 2014 Journal of pharmacopuncture Vol.17 No.3

Objectives: This study was performed to analyze the toxicity and to find the lethal dose of the test substance Water-soluble Carthami-flos pharmacopuncture (WCF) when used as a single intravenous-dose in 6-week-old, male and female Sprague-Dawley rats. Methods: The experiment was conducted at Biotoxtech according to Good Laboratory Practices. 20 female and 20 male Spague-Dawley rats were divided into 4 groups of 5 female and 5 male animals per group. The rats in the three experimental groups received single intravenous injections with 0.125-mL, 0.25-mL and 0.5-mL/animal doses of WCF, Groups 2, 3, and 4, respectively, and the control group, Group 1, received a single intravenous injection with a 0.5-mL dose of normal saline. Clinical signs were observed and body weight measurements were carried out for 14 days following the injections. At the end of the observation period, hematology, clinical chemistry, histopathological tests and necropsy were performed on the injected parts. Results: No deaths occurred in any of the groups. Also, no significant changes in body weight, hematological parameters or clinical chemistry test results between the control group and the experimental groups were observed. Visual inspection after necropsy showed no abnormalities. Histopathological tests on the injected parts showed no significant differences, except for Group 1 females; however, the result was spontaneous generation and had no toxicological meaning because it was not dose-dependent. Therefore, this study showed that WCF had no effect on the injected parts in terms of clinical signs, body weight, hematology, clinical chemistry, and necropsy. Conclusion: As a result of single intravenous-dose tests of the test substance WCF in 4 groups of rats, the lethal dose for both males and females exceeded 0.5 mL/animal. Therefore, WCF is a relatively safe pharmacopuncture that can be used for treatment, but further studies should be performed.

홍화자(紅花子)와 조경종옥탕(調經種玉湯)이 임신 흰쥐에 미치는 영향

김대준 ( Dae Jun Kim ),서부일 ( Bu Il Seo ) 대한본초학회 2013 大韓本草學會誌 Vol.28 No.3

Objectives:The present study has been undertaken to investigate the effects of Carthami Semen and Jogyeongjongok-Tang on pregnant rats. Method:In this experiment, the pregnant rats were administered by water extracts of Carthami Semen and Jogyeongjongok-Tang. The levels of weights, ALT, AST, ALP, BUN, creatinine, progesterone, Na and K in serum and reproductive indices of the rats were measured after treatment. Results: The levels of body weight gains were not significantly changed in comparison with Control group in Carthami Semen group and Jogyeongjongok-Tang group. In the levels of reproductive indices of the rats, the number of corpora lutea, implantation and viable fetuses, post-implantation loss, fetal weight and placental wight were not significantly changed in comparison with Control group in Carthami Semen group and Jogyeongjongok-Tang group. But pre-implantation loss was significantly increased in comparison with Control group in Carthami Semen group. The levels of BUN, creatine, ALT, AST and ALP were not significantly changed in comparison with Control group in Carthami Semen group and Jogyeongjongok-Tang group. The level of progesterone was not significantly changed in comparison with Control group in Carthami Semen group and Jogyeongjongok-Tang group. The levels of Na and K were not significantly changed in comparison withControl group in Carthami Semen group and Jogyeongjongok-Tang group. Conclusion:Reviewing these experimetal results, it appeared that Carthami Semen and Jogyeongjongok-Tang had not toxicity on pregnant rats.

韓國産 紅花子와 中國産 紅花子가 鎭痛 , 消炎 및 關節炎에 미치는 效果

서부일,이은숙,박지하,김상찬,변부형,최호영 대한본초학회 2001 大韓本草學會誌 Vol.16 No.1

This experimental study was done to compare the efficacy of Korean and Chinese Carthami Semen on analgesic effect and remedy of inflammation and arthritis. The results were as follows; 1. In analgesic action on the writhing syndrome in mice induced by 0.7% acetic acid, Korean and Chinese Carthami Semen extract group showed significant decrease in comparison with control group. And in analgesic action on the writhing syndrome in mice induced by Acetylcholine HC1, Korean and chinese Carthami Semen extract group showed significant decrease in comparison with control group. 2. In Anti-inflammatory effects, Chinese Carthami Semen extract group showed significant decrease at 150 and 180 minutes after inducing paw-edema in comparison with control group. 3. In the method of adjuvant arthritis, orally administered for 21 days, Korean Carthami Semen extract group showed significant inhibition of the hind paw edema after 1 day and 21 days. Whereas Chinese Carthami Semen extract group showed some reduction, but the results didn's show significant decrease. 4. In the level of leucocytes count, Korean and Chinese Carthami Semen extract group showed significant decrease in comparison with control group. And in the level of total protein. CRP and glucose, Korean and Chinese Carthami Semen extract group didn't showed significant change in comparison with control group.

홍화자(紅花子) 약침료법(藥鍼療法) 가미한 한방치료로 호전된 마미증후군(馬尾症候群) 환자(患者) 1례(例)

서보명 ( Bo Myung Seo ),이윤경 ( Yoon Kyoung Lee ),김성웅 ( Sung Woong Kim ),이세연 ( Sea Youn Lee ),서정철 ( Jung Chul Seo ),임성철 ( Seong Chul Lim ),정태영 ( Tae Youn Jung ),안희덕 ( Hee Duk An ),한상원 ( Sang Won Han ) 대한경락경혈학회 2005 Korean Journal of Acupuncture Vol.22 No.1

갑작스러운 요통과 양측하지통, 하지의 근력 감소, 대소변 장애 및 운동, 감각장애로 양방병원에서 마미증후군으로 진단받고 추긍판 절제술과 감압술을 시행 후 임상적인 호전을 보이지 않은 환자 1례에 대해 紅花子 藥鍼療法을 중심으로 복합적인 한방치료를 시행한 결과 요통에 대한 VAS와 요통점수표, ODI 항목에서는 각 항목 모두에서 현저한 호전 양상을 나타내었다. 운동기능 및 보행에 있어서 입원 당시에 보행기에 의지하여 보행하였는데 치료 후에는 단독으로 보행이 가능하고 일상적인 가벼운 생활도 가능해지게 되었다. 배뇨기능에 있어서도 자발적인 배뇨가 가능할 정도로 완전회복 되었다. 하지만 배변의 상태는 입원 당시와 치료 후에는 변의가 느껴지는 것 이외에 자발적인 배변은 이루어지지 않았다. 감각 기능의 회복에 있어서도 온통각, 압촉각과 하지 냉감은 호전 양상을 보였으나 안장 감각과 괄약근의 기능은 거의 회복되지 않았다. 본 연구를 기초로 하여 향후 수술이나 양방적인 치료 후에 발생할 수 있는 후유증의 관리에 있어서 紅花子 藥鍼에 대한 더욱 심도있는 후속 연구가 이루어져야 할 것이다. Objectives: This study was to investigate the effect of the Carthami Semen Herbal acupuncture therapy on a Cauda equina syndrome patient who has a complex of low back pain, bilateral sciatica, saddle anesthesia and motor weakness in the lower extremity and paraplegia with bladder and bowel incontinence. Methods: Oriental Medical Therapy was performed on the Cauda equina syndrome patient from July 15th 2004 to July 29th 2004. The patient was treated with Carthami Semen Herbal acupuncture at BL22, BL23, BL25, BL28 and GV3 in combination with herbal medicine and conventional body acupuncture. We evaluated The Visual Analog Scale(VAS), Improvement index, The Oswestry Diability Index(ODI), gaiting, dyschezia, bladder incontinence, duration of urination and area of anesthesia, Digital Infrared Thermographic Imaging(DITI) before and after treatment. Results: 1. After treatment, VAS, Improvement index, ODI were improved each from 10 to 2, from 21 to 73, from 333 to 166. 2. After treatment, gaiting, bladder incontinence, duration of urination and area of anesthesia and DITI were improved well, but dyschezia was remained. Conclusions: From this case it is thought Carthami Semen herbal acupuncture therapy is very effective to Cauda Equina Syndrome and further study is needed for the confirmation of the effect of Carthami Semen Herbal acupuncture.

紅花子 藥鍼療法을 가미한 한방치료로 호전된 馬尾症候群 患者 1例

서보명,한상원,서정철,이윤경,김성웅,이세연,임성철,정태영,안희덕 경락경혈학회 2005 Korean Journal of Acupuncture Vol.22 No.1

Objectives:This study was to investigate the effect of the Carthami Semen Herbal acupuncture therapy on a Cauda equina syndrome patient who has a complex of low back pain, bilateral sciatica, saddle anesthesia and motor weakness in the lower extremity and paraplegia with bladder and bowel incontinence.Methods: Oriental Medical Therapy was performed on the Cauda equina syndrome patient from July 15th 2004 to July 29th 2004. The patient was treated with Carthami Semen Herbal acupuncture at BL22, BL23, BL25, BL28 and GV3 in combination with herbal medicine and conventional body acupuncture. We evaluated The Visual Analog Scale(VAS), Improvement index, The Oswestry Diability Index(ODI), gaiting, dyschezia, bladder incontinence, duration of urination and area of anesthesia, Digital Infrared Thermographic Imaging(DITI) before and after treatment.Results:1. After treatment, VAS, Improvement index, ODI were improved each from 10 to 2, from 21 to 73, from 333 to 166.2. After treatment, gaiting, bladder incontinence, duration of urination and area of anesthesia and DITI were improved well, but dyschezia was remained. Conclusions:From this case it is thought Carthami Semen herbal acupuncture therapy is very effective to Cauda Equina Syndrome and further study is needed for the confirmation of the effect of Carthami Semen Herbal acupuncture. 갑작스러운 요통과 양측하지통, 하지의 근력감소, 대소변 장애 및 운동, 감각장애로 양방병원에서 마미증후군으로 진단받고 추궁판 절제술과 감압술을 시행 후 임상적인 호전을 보이지 않은 환자 1례에 대해 紅花子 藥鍼療法을 중심으로 복합적인 한방치료를 시행한 결과 요통에 대한 VAS와 요통점수표, ODI 항목에서는 각 항목 모두에서 현저한 호전 양상을 나타내었다. 운동기능 및 보행에 있어서 입원 당시에 보행기에 의지하여 보행하였는데 치료 후에는 단독으로 보행이 가능하고 일상적인 가벼운 생활도 가능해지게 되었다. 배뇨기능에 있어서도 자발적인 배뇨가 가능할 정도로 완전회복 되었다. 하지만 배변의 상태는 입원 당시와 치료 후에는 변의가 느껴지는 것 이외에 자발적인 배변은 이루어지지 않았다. 감각 기능의 회복에 있어서도 온통각, 압촉각과 하지 냉감은 호전 양상을 보였으나 안장 감각과 괄약근의 기능은 거의 회복되지 않았다. 본 연구를 기초로 하여 향후 수술이나 양방적인 치료 후에 발생할 수 있는 후유증의 관리에 있어서 紅花子 藥鍼에 대한 더욱 심도있는 후속 연구가 이루어져야 할 것이다.