The effect of autogenous tooth bone graft material without organic matter and type I collagen treatment on bone regeneration

김민구,이정한,김규천,황대석,김철훈,김복주,김정한,김욱규 대한악안면성형재건외과학회 2021 Maxillofacial Plastic Reconstructive Surgery Vol.43 No.-

Objectives: The aim of this study is to examine the effect of particulate autogenous tooth graft removed withorganic matter and type I collagen addition on bone regeneration and to validate the possibility of useful allograftmaterial for jaw defects. Material and methods: Autogenous tooth bone maker (Korean Dental Solution® KOREA) made particulateautogenous tooth not including organic matter. We used to the developed tooth grafts for experiment. Celladhesion test with hemacytometer and energy dispersive X-ray spectroscopy (Supra40 VP®, Carl Zeiss, Germany)analysis about the particulate autogenous tooth and type I collagen were performed. Rabbits were divided intothree groups: bone graft with organic matter (OM) removing particulate autogenous tooth group, bone graft withOM removing particulate autogenous tooth and type I collagen group, and a control group. Bone grafting wasperformed in rabbit’s calvaria. The rabbits were sacrificed at different interval at 1, 2, 4, and 6 weeks after bonegrafting for the histopathologic observation and observed the effect of bone regeneration by SEM, H-E & Massonstains, osteocalcin IHC staining. Result: In vitro cytopathological study showed affinity for cells, cell attachment pattern, and cell proliferation in theorder of control group, OM-removed and collagen-treated group, OM-removed particulate autogenous toothgroup. The results of the degree of mineralization were opposite to those of the previous cell experimental results,and the OM-removed group, OM-removed group and collagen-treated group were relatively higher than thecontrol group. Histopathologic analysis showed that vascularization and neonatal bone formation were higher inparticulate autogenous tooth group with removing OM and with addition of collagen than control group andgroup of OM removed only. Immunohistochemical analysis showed that osteocalcin (OSC) expression was notobserved in the control group, but at 4 weeks groups, OSC expression was observed the OM removed and OMremoved-collagen-treated particulate autogenous tooth, and the degree of expression was somewhat stronger ingroup of the OM removed and collagen additionally treated particulate autogenous tooth.

자가치아 뼈 이식재의 유용성에 관한 임상적 연구

한민우,이정근,Han, Min Woo,Lee, Jeong Keun 대한악안면성형재건외과학회 2013 Maxillofacial Plastic Reconstructive Surgery Vol.35 No.4

Purpose: This study is to evaluate the efficacy of the autogenous tooth bone graft material, clinically and radiologically, as related to implant installation. Methods: In oral and maxillofacial surgery department of Ajou University Hospital, guided bone regeneration (GBR), implant placement combined with GBR, sinus graft, implant placement combined with sinus graft, and defect filling were performed in 46 patients, using autogenous tooth bone. Among these, 66 implants were inserted with autogenous tooth bone. Implant stability quotient (ISQ) was measured by Osstell Mentor (Integration Diagnostics, Goteborg, Sweden) on 39 implants on the operation date and 4 months later, and on 21 implants 9months on the average at the final setting of restoration. Twenty-eight implants with GBR and sinus graft (GBR group: n=14, sinus graft group: n=14) were evaluated radiologically to measure the resorption of grafted autogenous tooth bone after loading. Results: The average initial stabilization of the installed implants was 67 ISQ, and the average secondary stabilization at 4 months later was 76. The average bone loss of GBR group as measured 8.0 months after application of prosthesis loading was 0.29 mm and the average bone loss of the sinus graft group as measured 7.6 months after application of prosthesis loading was 0.66 mm, respectively. In the histological assessment, formation of the new bone and continuous trabecular bone pattern was identified around autogenous tooth bone. Conclusion: Based on these results, we concluded that autogenous tooth bone is an excellent bone graft material that can substitute the autogenous bone.

Clinical Reference Strategy for the Selection of Treatment Materials for Maxillofacial Bone Transplantation: A Systematic Review and Network Meta-Analysis

Zhang Shuxin,Zhang Xinyi,Li Yanling,Mao Xuran,Liu Rui,Qi Yanxin,Lee Eui-Seok,Jiang Heng Bo 한국조직공학과 재생의학회 2022 조직공학과 재생의학 Vol.19 No.3

Bone graft materials have mixed effects of bone repair in the field of oral maxillofacial surgery. The qualitative analyses performed by previous studies imply that autogenous odontogenic materials and autogenous bone have similar effects on bone repair in clinical jaw bone transplantation. This retrospective systematic assessment and network meta-analysis aimed to analyze the best effect of clinical application of autogenous odontogenic materials and autogenous, allogeneic, and xenogeneic bone grafts in bone defect repair. A systematic review was performed by searching the PubMed, Cochrane Library, and other journal databases using selected keywords and Medical Subject Headings search terms. 10 Papers (n = 466) that met the inclusion criteria were selected. The assessment of heterogeneity did not reveal any overall statistical difference or heterogeneity (P = 0.051 > 0.05), whereas the comparison between autogenous and allogeneic bone grafts revealed local heterogeneity (P = 0.071 < 0.1). Risk of bias revealed nine unclear studies and one high-risk study. The overall consistency was good (P = 0.065 > 0.05), and the local inconsistency test did not reveal any inconsistency. The publication bias was good. The confidence regarding the ranking of bone graft materials after GRADE classification was moderate. The effects on bone repair in the descending order were as follows: autogenous odontogenic materials, xenogeneic bone, autogenous bone, and allogeneic bone. This result indicates that the autogenous odontogenic materials displayed stronger effects on bone repair compared to other bone graft materials. Autogenous odontogenic materials have broad development prospects in oral maxillofacial surgery.

가족치아를 이용한 골이식술

이지영(Ji-Young Lee),김영균(Young-Kyun Kim),엄인웅(In-Woong Um),최준호(Joon-Ho Choi) 대한치과의사협회 2013 대한치과의사협회지 Vol.51 No.8

The use of autogenous tooth bone graft material has been commercialized since 2008. Autogenous tooth bone grafts always require that the tooth of the patient be extracted, and thus, the use of graft material are limited in many cases. For solution of limitation in quantity and concurrent use of autogenous tooth bone graft material, the grafting of familial teeth has been suggested. It has the following advantages: the teeth of family members are used as bone graft materials, the genetic composition is identical, and potential genetic and infectious risks can be minimized. Because the teeth of family members are used, a good tissue affinity is obtained, and thus, superior bone generation rates compared to those observed for allogenic or xenogenic bones can be anticipated. We used familial tooth bone-graft materials for alveolar ridge augmentation, socket preservation, and maxillary sinus graft in some cases. In most cases, the impacted third molars of their children were prepared as bone graft material and were used for surgery. In one case, the impacted third molar from the patient`s brother was used as bone graft material. We obtained satisfactory result and these cases are reported herein.

Autotransplantation of an ectopic impacted premolar with sinus lift and allogenic bone graft

Pang, N. S.,Choi, Y. K.,Kim, K. D.,Park, W. Blackwell Publishing Ltd 2011 International endodontic journal Vol.44 No.10

<P><B>Pang NS, Choi YK, Kim KD, Park W.</B> Autotransplantation of an ectopic impacted premolar with sinus lift and allogenic bone graft. <I>International Endodontic Journal</I>, <B>44</B>, 967–975, 2011.</P><P><B>Abstract</B></P><P><B>Aim </B> This article presents a case report of autogenous tooth transplantation to a site which had insufficient bone volume using a sinus lift and allogenic bone graft.</P><P><B>Summary </B> An ectopic, fully impacted premolar tooth was autotransplanted from its ectopic impaction site into its original site, where there was insufficient recipient vertical bone volume because of sinus expansion. The deciduous tooth was extracted from the recipient site, and the sinus membrane detached and elevated via the alveolar socket. Allogenic bone grafting was performed, and the impacted premolar was transplanted into the prepared site. To improve adaptation, the recipient site was prepared using a rapid prototype tooth model, a replica tooth which allowed complete socket preparation in advanced of the actual removal of the donor tooth. The donor tooth was fixed with sutures and maintained for 17 days to allow physiologic movement. Root canal treatment was initiated 24 days after autotransplantation, and an intra‐canal medicament was used for 4 months. Canal filling was completed 5 months after autotransplantation. There was no root resorption of the transplanted tooth, and the grafted bone was well preserved and had no signs of infection.</P><P><B>Key learning points </B> <P> When the recipient bone volume is insufficient, autotransplantation can be preceded by bony augmentation.</P><P> The preparation of the recipient tooth socket using a tooth replica from CBCT reduces the extra‐oral time of the actual tooth and promotes better periodontal ligament healing.</P><P> Careful evaluation of the pulp status of the donor tooth is important in advance of timely endodontic treatment.</P></P>

자가치아를 이용한 골이식재의 임상적 유용성: 일차 보고

이정훈,김수관,문성용,오지수,김영균,Lee, Jeong-Hoon,Kim, Su-Gwan,Moon, Sung-Young,Oh, Ji-Su,Kim, Young-Kyun 대한악안면성형재건외과학회 2011 Maxillofacial Plastic Reconstructive Surgery Vol.33 No.2

Purpose: The purpose of this study was to evaluate the effectiveness of a novel bone grafting material using an autogeneous tooth (AutoBT) and provide the basis for its clinical application. The AutoBT contains organic and inorganic mineral components and is prepared from autogenous grafting material, thus eliminating the risk of immune reactions that may lead to its rejection. AutoBT can be used as bone material as is has both osteoinduction and osteoconduction activities at guided bone regeneration for implant placement and maxillary sinus graft. Methods: In a total of 63 patients, guided bone regeneration surgery was performed at the time of implant placement, and tissue samples were harvested at the time of the second surgery with the patient's consent. Results: There were no complications in guided bone regeneration using autogeneous tooth. Conclusion: We concluded that AutoBT underwent gradual resorption and was replaced by new bone of excellent quality via osteoinduction and osteoconduction.

Dentin Matrix Block의 치조골 복원 능력에 관한 임상적 연구

김경욱,Kim, Kyung-Wook 대한악안면성형재건외과학회 2013 Maxillofacial Plastic Reconstructive Surgery Vol.35 No.1

In the oral and maxillofacial area, bone defects are created by various reasons and demand for bone grafts, while dental implant implantation has been increased consistently. To solve these problems, there has been development of autogenous tooth-bone graft material (AutoBT$^{(R)}$, Korea Tooth Bank Co., Korea), and we have collected ground reasons to substitute free autobone graft with this material in clinical use. This autogenous tooth-bone graft material is produced in powder type and block type. Block type is useful in esthetic reconstruction of the defect site and vertical and horizontal augmentation of alveolar bone because this type has high strength value, well maintained shape and is less absorbed. Therefore, the author of this study gained favorable result by grafting the block type autogenous tooth-bone graft material after dental implant implantation on the bone defects of the mandibular molar extraction site. Moreover, the author represents this case with literature review after confirming bone remodeling on the computed tomography image and by histological analysis.

Evaluation of the Healing Process of Autogenous Tooth Bone Graft Material Nine Months after Sinus Bone Graft: Micromorphometric and Histological Evaluation

Kim, Young-Kyun,Jun, Sang-Ho,Um, In-Woong,Kim, Sooyeon Korean Association of Maxillofacial Plastic and Re 2013 Maxillofacial Plastic Reconstructive Surgery Vol.35 No.5

Micromorphometric and histological examinations were conducted with a collected tissue specimen nine months after sinus bone graft using autogenous tooth bone graft material (AutoBT). As a result of micro-computed tomographic analysis, the total bone volume (graft material+new bone) was 76.45%, and the proportion of new bone was 45.4%. The bone mineral density and the average Hounsfield Unit of new bone were 0.26 and 1,164.69, respectively. The histological examination showed that AutoBT particles were united well with new bone. AutoBT was considered to have excellent bone healing ability after sinus graft and bone density that can resist repneumatization.

즉시 탈회 치아이식재를 사용한 치조골 재건술

이은영(Eun-Young Lee) 대한치과의사협회 2016 대한치과의사협회지 Vol.54 No.5

Ideal autogenous or allogenic bone graft materials should provide 1) stabilization of blood clot, 2) scaffolds for cellular proliferation and differentiation, 3) release of osteogenic growth factors, 4) appropriate resorption profile for remodeling of new bone. Teeth, especially dentin, mostly contain hydroxyapatite and type I collagen which are similar to bone, and could be valuable graft material. Clinically teeth are used as calcined or demineralized forms. Demineralized form of dentin can be more effective as a graft material. But a conventional decalcification method takes time and long treatment time may give negative effects to various osteogenic proteins in dentin. Author used a new clinical method to prepare autogenous teeth, which could be grafted into the removal defects immediately after extraction using vacuum ultrasonic system. The process could be finished within two hours regardless of the form (powder, chip or block). Teeth were processed to graft materials in block, chip, or powder types immediately after extraction. It took 120 minutes to prepare block types and 40 minutes to prepare powder. Clinical cases did not show any adverse response and the healing was favorable. Rapid preparation of autogenous teeth with the vacuum ultrasonic system could make the immediate one-day extraction and graft possible.

자가치아골이식재의 결정구조 분석: X선 회절 분석

김경욱(Gyung-Wook Kim),여인성(In-Sung Yeo),김수관(Su-Gwan Kim),엄인웅(In-Woong Um),김영균(Young-Kyun Kim ) 대한구강악안면외과학회 2011 대한구강악안면외과학회지 Vol.37 No.3

This study evaluated the mineral crystalline structure of an autogenous tooth bone graft material. The crystalline structures of the autogenous tooth bone graft material enamel (AutoBT E+), dentin (AutoBT D+), xenograft (BioOss), alloplastic material (MBCP), allograft (ICB) and autogenous mandibular cortical bone were compared using XRD. The XRD pattern of AutoBT dentin and ICB was similar to that of autogenous bone.