Retinol-Binding Protein에 의해 유발된 실험적 포도망막염의 형태학적 변화

손영호,고명규,최준규 한양대학교 의과대학 1992 한양의대 학술지 Vol.12 No.2

Interphotoreceptor retinol-binding protein has been shown to be a potent uveitopathogenic in animal following immunization with this protein. The Retinol-binding protein which derived from the choriocapillaris interacts with membrane receptors of the retinal pigment epithelium and transfer all-trans retinol to the retinal pigment epithelium. Interphotoreceptor retinol-binding protein secreted by the photoreceptor is thought to mediate the transport of retinoid between the photoreceptor cell and retinal pigment epithelim. This study was performed to dlucidate the pathological features of the experimental retinitis induced by injection of retinol-binding protein in rabbits. 300 ug of retinol-binding protein was injected into the vitreous cavity through the pars plana in 6 black rabbits. Retinol-binding protein induced the inflammation of the uvea and retina, which started at date 2or 3 in six rabbits. The loss of some photoreceptor outer segment in retina with the intensive infiltration of the inflammatory cells such as lymphocytes, plasma cells and polymorphonuclear leukocytes in the retina particularly of the outer portion. These studies show that the injection of the RBP into the vitreous cavity can induce the uveouetinitis in the experimental model. Further studies will be required to elucidate the role of the retinol-binding protein in the induction of the experimental uveoretinitis.

Mapping, Tissue Distribution and Polymorphism of Porcine Retinol Binding Protein Genes (RBP5 and RBP7)

Gong, W.H.,Tang, Z.L.,Han, J.L.,Yang, S.L.,Wang, H.,Li, Y.,Li, K. Asian Australasian Association of Animal Productio 2008 Animal Bioscience Vol.21 No.11

The retinoids (vitamin A and its derivatives) play a critical role in vision, growth, reproduction, cell differentiation and embryonic development. Using the IMpRH panel, porcine cellular retinol binding protein genes 5 and 7 (RBP5 and RBP7) were assigned to porcine chromosomes 5 and 6, respectively. The complete coding sequences (CDS) of the RBP5 and RBP7 genes were amplified using the reverse transcriptase polymerase chain reaction (RT-PCR) method, and the deduced amino acid sequences of both genes were compared to human corresponding proteins. The mRNA distributions of the two genes in adult Wuzhishan pig tissues (lung, skeletal muscle, spleen, heart, stomach, large intestine, lymph node, small intestine, liver, brain, kidney and fat) were examined. A total of nine single nucleotide polymorphisms (SNPs) were identified in two genes. Three of these SNPs were analyzed using the polymerase chain reaction-restriction-fragment length polymorphism (PCR-RFLP) method in Laiwu, Wuzhishan, Guizhou, Bama, Tongcheng, Yorkshire and Landrace pig breeds. Association analysis of genotypes of these SNP loci with economic traits was done in our experimental populations. Significant associations of different genotypes of $RBP5-A/G^{63}$, $RBP5-A/G^{517}$ and $RPB5-T/C^{intron1-90}$ loci with traits including maximum carcass length (LM), minimum carcass length (LN), marbling score (MS), back fat thickness at shoulder (SBF), meat color score (MCS) and hematocrit (HCT) were detected. These SNPs may be useful as genetic markers in genetic improvement for porcine production.

12주간의 복합운동이 비만여성의 Retinol-binding Protein 4, 체지방률 및 동맥경직도에 미치는 영향

김용규(Yong-Kyu Kim),이정윤(Jeoung-Yun Lee),이서형(Seo-Hyung Lee) 한국생활환경학회 2017 한국생활환경학회지 Vol.24 No.2

The purpose of this study was to evaluate the effects of combined exercise on Retinol-binding protein 4(RBP-4), arterial stiffness and %body fat in obese middle-aged women. In this study, 24 subjects were divided into 2 groups, exercise(n=12) and control group(n=12), participated in this study for 12 weeks. The untrained obese women in I city voluteered to participate in this study. RBP-4, arterial stiffness and %body fat were analyzed before and after 12-weeks exercise intervention.. All data were expressed as mean±SD by using SPSS package program(win 23.0). The results of this study were as follows : RBP-4(P<.01), %body fat(P<.01) were significantly decreased in the exercise group. Arterial stiffness of right hand, left hand, right foot and left foot(P<.01) were significantly increased in the exercise group. It was concluded that combined exercise program favorably affects the RBP-4, %body fat and arterial stiffness. Therefore, 12weeks combinded exercise may be beneficial to prevent and improve obesity and cardiovascular disease in obese subjects.

Retinol-binding protein-4 was associated with sensitization to inhalant allergens in the elderly population

( Byung-keun Kim ),( Woo-jung Song ),( Bomi Seo ),( Ju-young Kim ),( Sae-hoon Kim ),( Hak C. Jang ),( Ki-woong Kim ),( Yoon-seok Chang ) 대한내과학회 2021 The Korean Journal of Internal Medicine Vol.36 No.2

Background/Aims: Recent evidence suggests an association between allergic sensitization and metabolic markers. However, this association has rarely been examined in the elderly. Retinol-binding protein-4 (RBP-4) is a recently identified adipokine that acts on the muscle and liver affecting insulin sensitivity. We evaluated the association between metabolic parameters and allergic sensitization in the elderly. Methods: We analysed the database of the Korean Longitudinal Study on Health and Aging cohort study conducted during 2005 to 2006. Atopy was identified by inhalant allergen skin prick test. Metabolic conditions were assessed using anthropometric indices and serum biomarkers such as fasting glucose, lipid, adiponectin, and RBP-4. Results: Among the 854 elderly subjects, 17.2% had atopy. Plasma RBP-4 levels were significantly higher in the atopic elderly than nonatopic elderly (p = 0.003). When RBP-4 percentiles were categorized as under three groups, the prevalence of atopy and current rhinitis increased significantly with percentiles of RBP-4 levels (p = 0.019 and p = 0.007, respectively). Log RBP-4 was associated with atopy (odds ratio [OR], 4.10; p = 0.009) and current rhinitis (OR, 2.73; p = 0.014), but not with current asthma (OR, 1.17; p = 0.824). Higher RBP-4 level in atopic elderly was also observed in current rhinitis patients. Atopy, but not current rhinitis, showed significant relationships with log RBP-4 levels in multivariate analyses adjusted for other metabolic markers including body mass index. Conclusions: RBP-4 positively associated with atopy in the general elderly population irrespective of other metabolic markers.

Retinol Binding Protein-Albumin Domain III Fusion Protein Deactivates Hepatic Stellate Cells

박상은,오준서,Soyoung Choi,이민구,임채승 한국분자세포생물학회 2012 Molecules and cells Vol.34 No.6

Liver fibrosis is characterized by accumulation of extracellular matrix, and activated hepatic stellate cells (HSCs) are the primary source of the fibrotic neomatrix and considered as therapeutic target cells. We previously showed that albumin in pancreatic stellate cells (PSCs), the key cell type for pancreatic fibrogenesis, is directly involved in the formation of vitamin A-containing lipid droplets, inhibiting PSC activation. In this study, we evaluated the anti-fibrotic activity of both albumin and retinol binding protein-albu-min domain III fusion protein (R-III), designed for stellate cell-targeted delivery of albu-min III, in rat primary HSCs and investigated the underlying mechanism. Forced expression of albumin or R-III in HSCs after passage 2 (activated HSCs) induced lipid droplet formation and deactivated HSCs, whereas point mutations in high-affinity fatty acid binding sites of albumin domain III abolished their activities. Exogenous R-III, but not albumin, was successfully internalized into and deactivated HSC-P2. When HSCs at day 3 after plating (pre-activated HSCs) were cultured in the presence of purified R-III, spontaneous activation of HSCs was inhibited even after passage 2, suggestive of a potential for preventive effect. Furthermore, treatment of HSCs-P2 with R-III led to a significant reduction in both cytoplasmic levels of all-trans retinoic acid and the subsequent retinoic acid signaling. Therefore, our data suggest that albumin deactivates HSCs with reduced retinoic acid levels and that R-III may have therapeutic and preventive potentials on liver fibrosis.

흰쥐 간장내 Ito 세포의 Retinol-Binding Protein 분포에 대한 면역세포화학적연구

장병준,윤화중 최신의학사 1992 最新醫學 Vol.35 No.11

Breeding and Genetics : Characterization of the Prolactin Receptor 3(PRLR3) and Retinol-Binding Protein 4(RBP4) Genes in the Birth Weight and Early Growth of Berkshire Pigs

( Chang Hee Do ),( Dong Hee Lee ),( Byung Wook Cho ) 한국동물자원과학회(구 한국축산학회) 2011 한국축산학회지 Vol.53 No.3

To investigate the influences of candidate genes on the birth weight and the early stages of life, genotyping of the prolactin receptor 3(PRLR3) and retinol-binding protein 4(RBP4) genes was performed in 156 and 141 Berkshire pigs, respectively. The frequency of both PRLR3 alleles A and a was 0.50. The frequencies of the RBP4 alleles B and b were 0.42 and 0.58, respectively. Neither locus was in Hardy-Weinberg equilibrium. No significant associations of the PRLR3 alleles with birth or weaning weights and of the RBP4 alleles with birth weight were observed. The proportions of the phenotype variances due to the genotypes of PRLR3 in the feeder weights was 4.0% and those of RBP4 in the weaning and feeder weights were 11.9 and 3.3%, respectively(P<0.05). The dominance effect of PRLR3 and RBP4 on feeder weights was 2.40 and -1.86kg, respectively(P< 0.01). The additive and dominance effects of RBP4 on weaning weights were 0.332 and -0.682kg, respectively(P<0.01). Even if no significant epistasis of PRLR3 and RBP4 was detected, a considerable trend of consistent positive epistasis estimates of AA/BB and Aa/Bb was observed for all traits. The results of this study may have a considerable impact on early-stage growth by both loci, and a selection strategy should be designed separately for each marker in Berkshire pigs

Effect of 4-Nonylphenol on the Gene Expression of Retinol-Binding Protein in the Rockfish, Sebastes schlegeli

조형구,정지현,이재용,김명희,한창희 한국발생생물학회 2006 발생과 생식 Vol.10 No.3

레티놀 결합 단백질(retinol-binding protein, RBP)은 고등 척추동물에서 혈류를 통해 특이적으로 레티놀을 표적세포에 운반해 주는 중요한 역할을 한다. 우리나라의 연안에 서식하고 있으며 산업적으로 중요한 조피볼락(Sebastes schlegeli)을 대상으로 4-nonylphenol(NP)가 RBP mRNA 발현에 미치는 영향을 구명하기 위해 간으로부터 cDNA library를 제작하고 RBP 단편의 염기서열을 분석하였다. 분석된 RB Retinol-binding protein(RBP) plays an important role in the specific transport of retinol to target cells through the blood stream in higher vertebrates. In order to clarify the effects of 4-nonylphenol(4-NP) on RBP mRNA expression in the rockfish, Sebastes schlegeli which is common in coastal waters of Korea and commercially important species, the cDNA library was constructed from the liver, and a partial fragment of the RBP gene was cloned. The deduced amino acid sequence from the RBP mRNA showed a high homology to the amino acid sequence from Sparus aurata(80%), Oncorhynchus mykiss(72%) or Anguilla anguilla(78%). Effects of 4-NP on RBP and vitellogenin(VTG) mRNA expression level in rockfish were examined by the northern blot analysis. In female and male rockfish injected with 4-NP(10 mg/kg BW, lower dose), there was no changes in the levels of VTG mRNA expression in the liver. The RBP mRNA levels, however, decreased at 48 hours after the injection in male. In the rockfish injected with 4-NP(25 mg/kg BW, higher dose), the level of VTG mRNA expression increased after 24 hours, regardless of sex. The level of RBP mRNA expression decreased at 48 hours after the injection in both sexes. These data indicate that estrogenic mimics such as 4-NP exhibit a contrasting effect on RBP and VTG gene expression in rockfish.

한국인 성인에서 관상동맥질환과 혈중 Retinol-Binding Protein 4 농도와의 연관성

김지훈 ( Ji Hoon Kim ),이은정 ( Eun Jung Rhee ),최은숙 ( Eun Suk Choi ),원종철 ( Jong Chul Won ),박철영 ( Cheol Young Park ),이원영 ( Won Young Lee ),오기원 ( Ki Won Oh ),김병진 ( Byung Jin Kim ),성기철 ( Ki Chul Sung ),김범수 ( B 대한당뇨병학회 2009 Diabetes and Metabolism Journal Vol.33 No.2

배경: 혈중 RBP-4는 새로이 밝혀진 아디포카인으로 최근 인슐린저항성과 대사증후군과의 연관성이 밝혀져 있다. 저자들은 한국인 성인에서 혈중 RBP-4 농도와 관상동맥질환과의 연관성에 관해서 분석하였다. 방법: 순환기내과에 흉통을 주소로 내원하여 관상동맥 조영술을 시행 받은 235명의 대상자(평균 연령 58세)에서 enzyme-linked immunosorbent assay를 이용하여 혈중 RBP-4 농도를 측정하였다. 모든 환자들에서 관상동맥 조영술을 시행하였고, 관상동맥질환의 중증도는 정상군, 단일혈관질환군, 두혈관질환군, 세혈관질환군으로 분류하였다. 모든 환자에서 신체계측, 공복혈당, 지질 농도 등을 측정하였다. 대사증후군 여부는, 허리둘레 대신 체질량지수를 적용한 AHA/NHLBI 진단기준으로 분석하였다. 결과: 관상동맥 조영술상 정상군은 101명(43.0%), 단일혈관질환군은 82명(34.9%), 두혈관질환군은 31명(13.2%), 세혈관질환군은 21명(8.9%)이었다. 관상동맥 협착을 가진 대상자들에서 정상군보다 높은 평균 연령(60.5±10.0 years), 공복 혈당(123.3±45.0 mg/dL), 낮은 고밀도 지단백 콜레스테롤 농도(49.0±13.2 mg/dL)를 보였으나, 혈중 RBP-4 농도는 관상동맥질환군과 정상군 사이에 의미있는 차이를 보이지 않았다. 협착된 혈관 수에 따른 각 변수들의 평균 수치를 비교하였는데, 정상군에서 세혈관질환군으로 갈수록 평균 연령, 공복 혈당 농도가 증가하였으나, 협착된 혈관 수에 따라서 혈중 RBP-4 농도는 의미있는 차이를 보이지 않았다. 각 대사성 지표들 중 중성지방 농도만이 혈중 RBP-4와 의미있는 연관성을 보였다(r=0.177, P=0.007). 결론: 한국인 성인에서 관상동맥질환 여부에 따른 혈중 RBP-4 농도는 협착된 혈관의 수나 질환 여부에 따라 차이가 없어서 동맥경화증에 미치는 영향은 적은 것으로 생각되나 대규모의 연구를 통한 결론이 내려져야 할 것이다. Background: A recently discovered adipokine, retinol-binding protein-4 (RBP-4), is reportedly associated with insulin resistance and metabolic syndrome. This study was performed to analyze the relationship between serum RBP-4 levels and coronary artery disease (CAD) in Korean adults. Methods: In 235 subjects (mean age 58 years) in whom coronary artery angiograms were performed due to complaints of chest pain, serum RBP-4 levels were measured by enzyme-linked immunosorbent assay. Coronary artery angiograms were performed in all subjects and the severity of CAD was assessed by the number of stenotic vessels. The presence of metabolic syndrome was defined by AHA/NHLBI criteria with body mass index substituted for waist circumference. Results: Coronary angiogram showed that 101 subjects (43%) had normal coronary vessel, 82 subjects (34.9%) had 1-vessel disease, 31 subjects (13.2%) had 2-vessel disease and 21 subjects (8.9%) had 3-vessel disease. Subjects with coronary artery stenosis showed a higher mean age (60.5±10.0 years), fasting glucose (123.3 mg±45.0 mg/dL) and lower mean value for high-density lipoprotein cholesterol (HDL-C) level (49.0±13.2 mg/dL), although serum RBP-4 levels were not significantly different between those with and without CAD. Mean age and fasting glucose level increased significantly as the number of stenotic vessels increased, although serum RBP4 level showed no significant differences among the different groups. Among the metabolic parameters, only serum triglyceride levels showed a significant correlation with serum RBP-4 levels. Conclusion: There was no difference in mean serum RBP-4 levels between subjects with or without coronary artery disease in Korean adults. Further studies are warranted to draw a clear conclusion on the effect of RBP-4 on atherosclerosis. (Korean Diabetes J 33:105-112, 2009)

Original Articles : Enhanced A-FABP expression in visceral fat: potential contributor to the progression of NASH

( Min Yong Yoon ),( Jun Mo Sung ),( Chang Seok Song ),( Won Young Lee ),( Eun Jung Rhee ),( Jun Ho Shin ),( Chang Hak Yoo ),( Seoung Wan Chae ),( Ja Yeon Kim ),( Wook Jin ),( Yong Kyun Cho ) 대한간학회 2012 Clinical and Molecular Hepatology(대한간학회지) Vol.18 No.3

Background/Aims: Adipose tissue is an active endocrine organ that secretes various metabolically important substances including adipokines, which represent a link between insulin resistance and nonalcoholic steatohepatitis (NASH). The factors responsible for the progression from simple steatosis to steatohepatitis remain elusive, but adipokine imbalance may play a pivotal role. We evaluated the expressions of adipokines such as visfatin, adipocyte-fatty-acid-binding protein (A-FABP), and retinol-binding protein-4 (RBP-4) in serum and tissue. The aim was to discover whether these adipokines are potential predictors of NASH. Methods: Polymerase chain reaction, quantification of mRNA, and Western blots encoding A-FABP, RBP-4, and visfatin were used to study tissue samples from the liver, and visceral and subcutaneous adipose tissue. The tissue samples were from biopsy specimens obtained from patients with proven NASH who were undergoing laparoscopic cholecystectomy due to gallbladder polyps. Results: Patients were classified into two groups: NASH, n=10 and non-NASH, n=20 according to their nonalcoholic fatty liver disease Activity Score. Although serum A-FABP levels did not differ between the two groups, the expressions of A-FABP mRNA and protein in the visceral adipose tissue were significantly higher in NASH group than in non-NASH group (104.34 vs. 97.05, P<0.05, and 190.01 vs. 95.15, P<0.01, respectively). Furthermore, the A-FABP protein expression ratio between visceral adipose tissue and liver was higher in NASH group than in non-NASH group (4.38 vs. 1.64, P<0.05). Conclusions: NASH patients had higher levels of A-FABP expression in their visceral fat compared to non-NASH patients. This differential A-FABP expression may predispose patients to the progressive form of NASH. (Clin Mol Hepatol 2012;18:279-286)