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배양된 영양배엽세포에서 저산소 상태가 혈관내피 성장인자, 섬유아세포 성장인자에 미치는 영향
이영 ( Young Lee ),신종철 ( Jong Chul Shin ),양동은 ( Dong Eun Yang ),문희봉 ( Hee Bong Moon ),이귀세라 ( Gui Sera Lee ),김사진 ( Sa Jin Kim ),이종승 ( Jong Seung Lee ),김창이 ( Chang Ee Kim ),김수평 ( Soo Pyung Kim ) 대한산부인과학회 2003 Obstetrics & Gynecology Science Vol.46 No.3
목적 : 임신초기 인간의 태반에서 분리하여 배양한 영양배엽세포에서의 VEGF 및 bFGF유전자의 mRNA발현에 저산소상태가 미치는 영향을 연구하고자 시행하였다. 연구 방법 : 임신초기 (6-10주)에서 얻은 태반조직 5예를 이용하여 영양배엽세포를 분리한 후 정상산소상태 (5% CO2, 95% humid air in incubator) 및 저산소상태 (MERCK, 1% O2, 99% CO2)에서 각각 24시간, 48시간 및 72시간씩 배양하였다. 각각의 Objective : To investigate whether the hypoxic condition influences on the expression of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) mRNA in the cultured human trophoblast. Methods : Trophoblasts were isolated from
간 세포암에서 VEGF, TGF-β1, b-FGF 발현의 의의
김성용,남충현,주종우,채만규,백무준,이문수,김형철,안현철,김홍수,김창진,김창호 순천향의학연구소 2001 Journal of Soonchunhyang Medical Science Vol.7 No.1
Purpose: Angiogenesis is important for the proliferation and the metastasis of solid tumors. The growth of a solid tumor is widely recognized to depend on the process of neovascularrozation. Without angiogenesis, tumors cease to grow beyond even a few milimeters in diameter. It has been shown that tumor vascular density is an independent prognostic marker in several types of human tumors and is known to correlate with poor prognosis. To date, many angiogenic factors have been identified, such as transforming growth factor-α(TGF-α), transforming growth factor-β(TGF-β), fibroblast growth factor family(FGF), vascular endothelial growth factor(VEGF), platelet derived endothelial cell growth factor(PD-ECGF), tumor necrosis factor-α(TNF-α), and angiogenin. Hepatocellular carcinoma(HCC) is the second most common tumor in Korean males and is known as a typical hypervascular tumor with frequent portal vein invastion. The authors identified the expreesion of VEGF, TGF-β1, and b-FGF in HCC specimens and evaluated the relationship between these growth factors and the clinicopathologic characteristics of HCC. Method: We reviewed the medical records of 30 patients who were diagnosed as hepatocellular carinoma treated with hepatic resection between January 1994 and December 1998 in Soonchunhyang University Chunan Hospital. The selection of the cases was decided according to the condition of paraffin block fixation. The prognostic factors such as age, sex, tumor size, concentration of serum α-fetoprotein, presence of liver cirrhosis, presence of tumor emboli in portal vein, TMN stage, amount of transfusion during the operation, hepatitis B virus(HBV) infection, and Edmonson-Steiner(E-S) grade were investigated. Relationship between the prognostic factors and the immunopathologic expression of the TGF-β1, b-FGF, and VEGF was examined. Result: Thirty patients (24 males, 6 females) were included in the current study. The patient's mean age was 50.6 years and the age ranged from 36 to 65 years. The mean size of the tumor was found to be 5.2cm. All the patients were follewed up for 7 to 63 months. Child's classification A patients were 23(76.7%)cases, B patients were 7(23.3%)cases, and C was none. Immunohistochemical staining of HCC tumor mass in VEGF expression patients were 17(56.7%), b-FGF expression patients were 10(33.3%), and TGF-β1 expression patients were 10(33.3%). VEGF expression or more than one positive expression among the three factors correlated with tumor size and the stage of HCC but did not correlated with other clinicopathological characteristics. TGF-β1 and b-FGF did not correlate with any clinicopathological characteristics. Conclusion: The results suggest that the expression of VEGF or more than one positive expression among the three factors in HCC cells may be a significant prognostic factor of HCC.