肝炎에서 增殖된 α-smooth muscle actin의 表現 樣相

기근홍,조국형,설주문 朝鮮大學校 附設 醫學硏究所 1992 The Medical Journal of Chosun University Vol.17 No.2

α-smooth muscle actin is an actin isoform typical of smooth muscle cells, and could be a reliable marker for phenotypic modulation toword smooth muscle cells. Six major isoforms of actin have been discribed. The materials for our study consisted of 50 chronic active hepatitis (10 mild, 15 moderate, 15 severe, and 10 precirrhotic change) and 25 chronic persisitent hepatitis, that biopsied for diagnosis at Chosun University Hospital from January, 1989 to May, 1992. All cases were performed of hematoxylin-eosin stain, PAS stain, Masson-trichrome stain, reticulin stain, and immunohistochemical stain of α-smooth muscle actin, vimentin and desmin by ABC method using micro-probe system. Immunoreactivity was evaluated and compared with the reactivity of a-smooth muscle actin, vimentin and desmin. The results obtained were as follows 1. In the chronic persistent hepatitis, α-smooth muscle actin positive cells appeared in the vascular smooth muscle cells of perisinusoidal spaces and limiting plate area. 2. With the progression of grade in chronic active hepatitis, α-smooth muscle actin positive cells were recognized in the perisinusoidal spaces, fibrotic area, and area of regenerating nodule. In conclusion, α-smooth muscle actin is a good marker for the detection of fibroblasts, myofibroblasts and myofibroblast-like cells. So α-smooth muscle actin was helpful diagnostic aid in differentiation of chronic active hepatitis and chronic persistent hepatitis.

병기 ⅠB-ⅡB 자궁경부암에서의 CD44v4/5, CD44v6, α-Smooth Muscle Actin과 nm23의 발현

장희경,허만하,김동휘,박은동 대한병리학회 1997 Journal of Pathology and Translational Medicine Vol.31 No.6

손상 받은 각막 상피세포가 중배엽 줄기세포의 분화에 미치는 영향

신미선,홍현숙,손영숙,김재찬,Mi Sun Shin,M,S,Hyun Sook Hong,M,S,Young Sook Son,Ph,D,Jae Chan Kim,M,D 대한안과학회 2007 대한안과학회지 Vol.48 No.3

Purpose: To identify the effects of microenvironmental changes caused by human corneal epithelial damages to characteristics or differentiation of human mesenchymal stem cells (hMSCs). Methods: Artificial corneal damage was induced onto a cultured monolayer of human corneal epithelial cells. hMSCs were then co-cultured with damaged human corneal epithelial cells (dIHCE). Morphological changes in the co-cultured hMSCs were observed. To elucidate the differentiation of hMSCs into corneal keratocytes or epithelial cells, the expressions of α-smooth muscle actin, keratin-3/-12, and E-cadherin were confirmed by immunofluorescence. Results: hMSCs co-cultured with dIHCE showed enhanced adherence in the neighborhood of dIHCE and morphological change into dendritic shapes at 6 days post-seeding. Although the expression of α-smooth muscle actin, known as hMSCs marker, significantly decreased at the dIHCE-contacted site of hMSCs; there were no expressional changes on keratin-3/-12 and E-cadherin, the markers of corneal epithelial cells. Interestingly, positive expression of corneal epithelial marker keratin-3/-12 was observed in dIHCE co-cultured hMSCs. hMSCs co-cultured with normal human corneal epithelial cells (nIHCE) were unable to attach, and showed no change in the expression of α-smooth muscle actin. Conclusions: It is proposed that dIHCE causes a morphological change in hMSCs, and decreased expression of α-smooth muscle actin. These results suggest that dIHCE can affect a change in the characteristics and differentiation of hMSCs.

중간엽 줄기세포의 평활근 세포로의 분화에서 LPS에 의해 활성화된 대식세포의 역할

Mi Jeong Lee(이미정),Eun Kyoung Do(도은경),Jae Ho Kim(김재호) 한국생명과학회 2013 생명과학회지 Vol.23 No.1

인체 중간엽 줄기세포는 지방세포, 골세포, 연골세포, 근육세포 등 여러 형태의 세포로의 분화되는 것이 특징이다. 특히, 최근 연구 결과를 살펴 보면, 중간엽 줄기세포는 생체 내에서 조직 특이적인 세포 형태로 분화 된다. 본 연구에서는 염증 상태에 존재하는 중간엽 줄기 세포가 혈관 형성에 관여하는지 알아보고, 염증 상태에 존재하는 줄기세포의 역할을 규명하고자 본 연구를 진행하였다. 생체 내 염증 상태와 유사한 환경을 만들고자, 강력한 염증 유발 물질인 LPS를 대식세포에 처리하여 그 배양액을 중간엽 줄기세포에 처리하여 변화를 관찰하였다. LPS배양액을 처리한 중간엽 줄기세포는 평활근 세포로 분화가 촉진되는 것을 확인하였으며, LPS 배양액에 존재하는 분화 유도 물질이 PGF2α임을 확인하였다 이에 본 연구결과를 통해 염증 상태에서 존재하는 중간엽 줄기세포는 평활근 세포로의 분화가 촉진되는 것을 확인하였다. 본 연구는 염증성 미세환경 내에 존재하는 중간엽 줄기세포가 평활근 세포로 분화가 유도됨을 확인하였고, 혈관 형성을 촉진하는데 영향을 미칠 수 있음을 제시한다. Human adipose-derived mesenchymal stem cells (hMSCs) are highly useful for vascular regeneration of injured or inflamed tissue. Lipopolysaccharide (LPS) is a potent activator of macrophages and stimulates macrophages to release inflammatory cytokines. In the present study, we explored the role of LPS-activated macrophages in the differentiation of hMSCs to smooth muscle cells (SMCs). We demonstrated that conditioned medium from LPS-induced macrophages (LPS CM) stimulates differentiation of hMSCs to SMCs, as evidenced by increased expression of smooth muscle-specific markers, including alpha-smooth muscle actin (α-SMA), smooth muscle-myosin heavy chain, and calponin. LPS induced the secretion of PGF2α from macrophages, and PGF2α treatment stimulated expression levels of SMC-specific markers in hMSCs. Furthermore, small interfering RNA-mediated silencing of the PGF2α receptor inhibited LPS CM-stimulated α-SMA expression. These results suggest that LPS-activated macrophages promote differentiation of hMSCs to SMCs through a PGF2α-dependent mechanism.

Red ginseng extract protects against carbon tetrachloride-induced liver fibrosis

Sung Hwan Ki,Ji Hye Yang,Sae Kwang Ku,Sang Chan Kim,Young Woo Kim,Il Je Cho 고려인삼학회 2013 Journal of Ginseng Research Vol.37 No.1

Korean red ginseng, the processed root of Panax ginseng Meyer, has been frequently used for various therapeutic purposes in oriental medicine. The present study investigated the possible effect of Korean red ginseng extract (RGE) for the treatment of liver fibrosis in mice injected with carbon tetrachloride (CCl4) for 4 wk. Liver injuries were assessed by blood biochemistry and histopathology in mice treated with CCl4 alone or CCl4+ RGE (30, 100, and 300 mg/kg). Concomitant treatment with RGE and CCl4 (three times/wk for 4 wk) effectively inhibited liver fibrosis as evidenced by decreases in plasma alanine and aspartate aminotransferases, as well as by the percentages of degenerative regions, numbers of degenerative hepatocytes, and collagen accumulation in hepatic parenchyma. Treatment with CCl4 for 4 wk increased mRNA levels of transforming growth factor b1 and plasminogen activator inhibitor 1 in fibrogenic liver, whereas RGE (30, 100, and 300 mg/kg) significantly blocked the induction of fibrogenic genes by CCl4. Similarly, RGE also prevented transforming growth factor b1-mediated induction of fibrogenic genes in human hepatic stellate cell lines. More importantly, RGE markedly reduced the number of a-smooth muscle actin-positive cells in liver tissue. This study implies that RGE efficaciously protects against the liver fibrosis induced by chronic CCl4 treatment, and may therefore have potential to treat liver disease.

Podoplanin, α-Smooth Muscle Actin or S100A4 Expressing Cancer-Associated Fibroblasts Are Associated with Different Prognosis in Colorectal Cancers

최송이,윤순만,이상전,윤세진,성노현,이택규,김나영,이은정,채희복,박선미 대한의학회 2013 Journal of Korean medical science Vol.28 No.9

The interactions between the tumor microenvironment and tumor cells determine the behavior of the primary tumors. Whether cancer-associated fibroblasts (CAF) have a tumor progressive or a protective role likely depends on the type of tumor cells and the CAF subpopulation. In the present study, we analyzed the prognostic significance of CAF subpopulations in colorectal cancer (CRC). CAF phenotypes were analyzed in 302 CRC patients by using antibodies against podoplanin (PDPN), α-smooth muscle actin (α-SMA),and S100A4. The relationship between the CAF phenotypes and 11 clinicopathological parameters were evaluated and their prognostic significance was analyzed from the disease-free and overall survival times. We observed that at the tumor invasive front,PDPN CAFs were present in 40% of the cases, and S100A4 or α-SMA CAFs were detected in all the cases. PDPN/S100A4 and α-SMA/S100A4 dual-stained CAFs were observed in 10% and 40% of the cases, respectively. The PDPN+ CAFs were associated with 6 favorable clinicopathological parameters and prolonged disease-free survival time. The PDPN−/α-SMAhigh CAFs were associated with 6 aggressive clinicopathological parameters and tended to exhibit shorter disease-free survival time. On the other hand, the PDPN−/S100A4high CAFs were associated with 2 tumor progression parameters, but not with disease prognosis. The PDPN+ CAF phenotype is distinct from the α-SMA or S100A4 CAFs in that it is associated with less aggressive tumors and a favorable prognosis, whereas the PDPN−/α-SMAhigh or PDPN−/S100A4high CAFs are associated with tumor progression in CRC. These findings suggest that CAFs can be a useful prognostic biomarker or potential targets of anti-cancer therapy in CRC.

Primary leiomyoma of the ovaries in a dog

Hyeon-Wook Lee, Kyung-Ku Kang, Chang-Woo Min, Ah-Young Kim, Eun-Mi Lee, Eun-Joo Lee, Myeong-Mi Lee, Sang-Hyeob Kim, Soo-Eun Sung, Kyu-Shik Jeong 충북대학교 동물의학연구소 2014 Journal of Biomedical and Translational Research Vol.15 No.1

We would like to report a case of leiomyoma of the ovaries in a dog. Leiomyoma is commonly seen in the vagina in dogs. However, it is a very rare neoplasm in the ovaries. As there have only been a few reported cases, this report provides valuable information on veterinary medicine and pathology. Masses found in the ovaries need to be differentiated from other ovarian tumors. Therefore, we describe the gross, histopathological, and immunohistochemical features of a case of ovarian leiomyoma in a 10-year-old female Yorkshire Terrier dog. The mass on the right of the uterus was found accidentally by pelvic ultrasonography. Laparatomy revealed a large multi-nodulated ovarian mass. Grossly, cut surfaces of the mass showed multiple firm whitish nodules in the cortex and bloody loose connective tissue in the medulla. Histopathologically, the cortex of the mass was composed of spindle cells forming interlacing fascicles. The cells had elongated, blunt-ended nuclei and eosinophilic cytoplasm as detected by hematoxylin and eosin staining. Immunohistochemical stained sections were immunoreactive for α-smooth muscle actin and desmin but negative for vimentin and S-100. Therefore, differential diagnosis confirmed leiomyoma based on morphology and positive staining for α-smooth muscle actin and desmin.

FSP-1 expression in cancer cells is relevant to long-term oncological outcomes in nonmetastatic colorectal cancer

Sun Bin Im,Jae Min Cho,Han Byul Kim,신동훈,Myeong Sook Kwon,In Young Lee,손경모 대한종양외과학회 2022 Korean Journal of Clinical Oncology Vol.18 No.2

Purpose: Recent studies have revealed that the expression of cancer-associated fibroblast (CAF) activation biomarkers in cancer cells is associated with clinical outcomes in patients with certain types of malignant tumors. However, whether the expression of CAF activation biomarkers affects the prognosis of colorectal cancer (CRC) has not been fully elucidated. This study aimed to evaluate the association between the expression of CAF activation biomarkers in cancer cells with cancer invasion and long-term oncological outcomes in patients with CRC. Methods: Cancer specimens obtained from 135 patients with stage I–III CRC were examined using immunohistochemical staining to evaluate the expression of fibroblast specific protein-1 (FSP-1), fibroblast activation protein α (FAPα), α-smooth muscle actin (α-SMA), and vimentin in cancer cells. Results: FSP-1 expression in cancer cells was significantly associated with lymphatic invasion, perineural invasion, tumor (T) status, and lymph node (N) status. FAPα expression in cancer cells was significantly associated with lymphatic invasion. On univariate and multivariate analyses, FSP-1 and α-SMA expression in cancer cells were associated with a short 10-year overall survival (OS) and high 10-year systemic recurrence (SR), respectively. Tumor budding was associated with a short 10-year OS. However, FAPα and vimentin did not contribute to the prognosis in this study. Conclusion: In this study, we found that FSP-1 expression in cancer cells was related to cancer invasion. Additionally, FSP-1 and α-SMA expression in cancer cells was associated with 10-year OS and SR, respectively. Therefore, these markers may be used as predictors of long-term oncological outcomes in patients with CRC.

흰쥐에서 임신이 무릎관절 구축에 미치는 영향

김준성,강세윤 大韓再活醫學會 1999 Annals of Rehabilitation Medicine Vol.23 No.6

Primary leiomyoma of the ovaries in a dog

이현욱,강경구,민창우,김아영,이은미,이은주,이명미,김상협,성수은,정규식 충북대학교 동물의학연구소 2014 Journal of Biomedical and Translational Research Vol.15 No.1

We would like to report a case of leiomyoma of the ova- ries in a dog. Leiomyoma is commonly seen in the vagina in dogs. However, it is a very rare neoplasm in the ovaries. As there have only been a few reported cases, this report provides valuable information on veterinary medicine and pathology. Masses found in the ovaries need to be differen- tiated from other ovarian tumors. Therefore, we describe the gross, histopathological, and immunohistochemical features of a case of ovarian leiomyoma in a 10-year-old female Yorkshire Terrier dog. The mass on the right of the uterus was found accidentally by pelvic ultrasonography. Laparatomy revealed a large multi-nodulated ovarian mass. Grossly, cut surfaces of the mass showed multiple firm whit- ish nodules in the cortex and bloody loose connective tissue in the medulla. Histopathologically, the cortex of the mass was composed of spindle cells forming interlacing fascicles. The cells had elongated, blunt-ended nuclei and eosinophilic cytoplasm as detected by hematoxylin and eosin staining. Immunohistochemical stained sections were immunoreac- tive for α-smooth muscle actin and desmin but negative for vimentin and S-100. Therefore, differential diagnosis con- firmed leiomyoma based on morphology and positive stain- ing for α-smooth muscle actin and desmin.