원색도감(原色圖鑑) 발간(發刊)을 위한 한국산(韓國産) 자낭균류(子囊菌類)의 분류학적(分類學的) 연구(硏究)

이용보,이지열,Lee, Yong-Bo,Lee, Ji-Yul 한국균학회 1982 韓國菌學會誌 Vol.10 No.3

본연구(本硏究)는 원색(原色) 한국산(韓國産) 버섯도감발간사업(圖鑑發刊事業)을 위하여 1981년(年) 7월(月) 1일(日)부터 1982년(年) 6월(月) 30일(日)까지 1년간(間) 경기도(京畿道) 광육임업시험장(光陸林業試驗場)을 주(主)로 하고, 경기도(京畿道), 용문산(龍門山), 강원도(江原道) 치악산(雉岳山), 충청북도(忠淸北道) 용이산(俗離山), 경상남도(慶尙南道) 지이산(智異山), 전라남도(全羅南道) 무등산(無等山), 제주도(濟州道) 한라산(漢拏山) 등지(等地)에서 신선(新鮮)한 자낭균류(子囊菌類)를 채집(採集) 촬영(撮影)하여 원색(原色)슬라이드와 건조표본(乾燥標本)을 작성(作成)하고 분류학적(分類學的) 연구(硏究)를 완성(完成)하였다. 조사(調査)된 결과(結果)에 의하면 우리나라에서 밝혀진 총고등균류수(總高等菌類數)인 620여종중(餘種中) 흔히 볼 수 있는 균류(菌類)와 한국미기록(韓國未記錄) 자낭균류(子囊菌類) 101종(種)의 원색(原色) 슬라이드와 분류상(分類上)의 검색표(檢索表)를 완성(完成)하였다. 이들은 10목(目) 19과(科) 50속(屬) 101종(種)에 속(屬)하고, 또 이들의 세계적(世界的) 분포(分布)가 밝혀졌다. 그들 중 Peziza vesiculosa 큰 주발버섯, Rhizina inflata 땅해파리, Gelasinospora longispora, Sordaria fimicola, Cantharomyces exiguus, Dichomyces biformis, D. furcifer, D. homalotae, D. hybridus, D. vulgatus, Dioicomyces anthici, Enathromyces indicus, Laboulbenia borealis, L. brachconychi, L. cristata, L. exigua, L. fusciculata, K. filifera, L. flagellata, L. rougetii, L. tachys, L. vulgaris, L. yoshidai, Rickia ancylopi, R. papuana 그리고 R. peyerimhoffii의 26종(種)은 한국산(韓國産) 미기록종(未記錄種)이며, 그 보통명(普通名)과 기재(記載)를 하였다. 본론문(本論文)에서는 예정(豫定)대로 101종(種)의 자낭균류중(子囊菌類中) 원색도판(原色圖版) 1면(面)만을 게재(揭載)하였다. This study has been carried out for the publication of colored illustrations of Korean fungi. The fresh fungi of Ascomycetes were collected and photographed, for the most part, at Gwangneung, Kyonggi Province, Mt. Yongmun in Kyonggi Province, Mt. Sokri in Chungcheong Province, Mt. Jiyee in Kyeongsang Province, Mt. Mudeug in Jeonra Province, and Mt. Hanra in Jeuju Island from July 1, 1981 to June 31, 1982. These higher fungi were made into colored slides and dried specimens, then classified. According to the investigated result, colored slides and classification for the common and new to Korea 101 species were completed among 620 spp. which were known all of the fungi in Korea. They were included 10 oders, 19 families, 51 genera and 101 species. Their world distributions were investigated. Twenty six species of them, that is, Peziza vesiculosa, Rhizina inflata, Gelasinospora longispora, Sordaria fimicola, Cantharcmyces exiguus, Dichomyces biformis, D. furcifer, D. homalotae, D. hybridus, D. vulgatus, Dioicomyces anthici, Enathromyces indicus, Laboulbenia borealis, L. brachconychi, L. cristata, L. exigua, L. fusciculata, L. filifera, L. flagellata, L. rougetii, L. tachys, L. vulgaris, L. yoshidai, Rickia ancylopi, R. papuana and R. peyerimhoffii were found to be new in Korea. In addition their common names and descriptions prepared. According to study plan, this paper pressed only one page in colored plate among 101 species.

한국산 개암버섯속의 미기록종 보고

석순자 ( Soon Ja Seok ),권순우 ( Soon Woo Kwon ),박인철 ( In Cheol Park ),김양섭 ( Yang Sup Kim ),유관희 ( Kwan Hee Yoo ) 한국균학회 2012 韓國菌學會誌 Vol.40 No.4

본 연구는 한국산 주름버섯목의 종 다양성과 분포상을 조사하는 과정에서 경기도 용문산에서 한국미기록 긴대개 암버섯 "Hypholoma elongatum(Pers.) Ricken"을 채집하여 형태적 특성에 준하여 분류동정 하였다. 자실체의 형태적 특징에 대한 상세한 기술과 미세구조를 도해하고 한국명을 신칭하여 보고하고자 한다. 본 연구에 사용된 표본은 국립농업과 학원 식물균류표본보존센타(HCCN)에 보존되어 있다. ABSTRACT: Different mushroom species have collected from various areas and their taxonomic identification was carried out for species diversity. Among them, Hypholoma elongatum (Pers.) Ricken that was collected from Yongmoon mountain of Gyonggi in Korea was confirmed as new collection in Korea and registered here with descriptions and Korean common name. All collections cited here are deposited in the Herbarium Conservation Center of National Academy of Agricultural Sciences.

한국에서 채집된 가지 흰가루병균의 실체

조성은 ( Sung-eun Cho ),최인영 ( In-young Choi ),신현동 ( Hyeon-dong Shin ) 한국균학회 2017 韓國菌學會誌 Vol.45 No.2

한국에서 가지 흰가루병균으로 Erysiphe cichoracearum, Leveillula taurica, Sphaerotheca fusca 모두 3종이 기록되어 있다. E. cichoracearum은 1969년에 기록된 이후 한국에서 발생하는 가지 흰가루병균으로 여긴다. 1998년에는 L. taurica가 가지 뒷면흰가루병균으로 기록 되었으나, 이후에 추가적인 발생기록은 없었다. 2002년에는 S. fusca가 가지 흰가루병균으로 보고되었다. 필자들은 총 22점의 가지 흰가루병균 시료를 채집하여 현미경 관찰 및 염기서열 분석을 실시하였으며, 그 결과 모두 Podosphaera xanthii로 동정하였다. 따라서 한국에서 가지 흰가루병균은 P. xanthii로 표기하는 것이 옳으며, 시설재배에서 드물게 발견되는 L. taurica는 뒷면흰가루병균으로 구별하는 것이 맞다. 반면에, E. cichoracearum (= Golovinomyces cichoracearum)은 가지 흰가루병균으로 표본이 보존되지 않았으며 이후에 채집되지도 않았다. 더구나 가지에서 기록한 E. cichoracearum의 기재는 일반적인 형태적 변이의 폭을 크게 벗어난다. 따라서 E. cichoracearum 흰가루병균의 존재에 대한 과거의 기록은 오류로 생각 된다. 결국 P. xanthii가 한국의 가지에서 발병하는 흰가루병의 주요 병원균으로 판단되며, L. taurica는 드물게 발생되었던 것으로 판단된다. 이 총설에서는 가지 흰가루병균의 역사와 최신 분류체계에 대하여 자세히 기록하였다. Three species of powdery mildew (Erysiphales) on eggplant (Solanum melongena L.) have been listed in Korea, namely Erysiphe cichoracearum (now genus Golovinomyces), Leveillula taurica, and Sphaerotheca fusca (now genus Podosphaera; syn. Podosphaera xanthii). Since E. cichoracearum was recorded on eggplant for the first time in Korea in 1969, it has been regarded as a major powdery mildew agent on that plant. In 1998, the causal agent of powdery mildew on eggplant was recorded as L. taurica, then as S. fusca in 2002. During our extensive field surveys in Korea, we collected 22 samples of eggplant powdery mildews. Our microscopic observations and molecular sequence analyses showed that all of our samples belonged to the genus Podosphaera, in the absence of either E. cichoracearum or L. taurica, suggesting that P. xanthii is the dominant agent of powdery mildew disease on eggplants in Korea. As there have been no additional findings on L. taurica after the first report on the species, it seems to be a minor species that is rarely found in greenhouses. The presence of E. cichoracearum (syn. Golovinomyces cichoracearum s. lat.) on eggplants is questionable, as the morphological characteristics of E. cichoracearum in the original description of the Korean collection deviate from the morphological variations of this species. In addition, no herbarium material of E. cichoracearum remains. Consequently, it seems that P. xanthii is the main species of powdery mildew on eggplants, whereas L. taurica occurs rarely on eggplants, in Korea. This review provides the historical and recent taxonomy of eggplant powdery mildews in detail.

A new record of Trichocladium griseum in Korea: morphological and molecular characterization

( Setu Bazie Tagele ),( Thuong T. T. Nguyen ),( Sang Woo Kim ),( Mahesh Adhikari ),( Sun Kumar Gurung ),( Hyun Goo Lee ),( Byeong Heon Gwon ),( Han Jun Ju ),( San Kosol ),( Hyang Burm Lee ),( Youn Su 한국균학회 2019 韓國菌學會誌 Vol.47 No.2

A unrecorded species of Trichocladium, Trichocladium griseum, was isolated in 2017 during a survey of fungal diversity in Ulsan province, South Korea. This species was identified based on morphological characteristics and phylogenetic analysis of the internal transcribed spacer (ITS) rDNA and β-tubulin gene sequences. T. griseum has not yet been reported in South Korea. Thus, we report for the first time a new record of Trichocladium griseum in Korea, and we include the descriptions and morphological illustrations of this fungus.

First Report of Allantophomopsiella pseudotsugae Isolated from Soil in Korea

( Ally Hassan Wajihi ),( Seung-yeol Lee ),( Kallol Das ),( Ahn-heum Eom ),( Hee-young Jung ) 한국균학회 2019 韓國菌學會誌 Vol.47 No.1

A fungal isolate designated 17E029 was isolated from a soil sample in Jeju, Korea. The strain was similar to other Allantophomopsiella species in its morphological characteristics such as grey mycelia, conidiophore, and conidia sizes. The isolate produced aerial mycelia, which appeared grey on the reverse side of the media surfaces and turned black on the front side of the colonies. The conidiophores emanating from the hyphae were hyaline, grey, aseptate, branched, and 6.7~9.2 × 1.8~2.5 μm. Conidiogenous cells were ovoid to subcylindrical, discrete, guttulate, and hyaline. Conidia were hyaline, aseptate, smooth, guttulate, oval to subcylindrical, irregular in shape, and 6.0~7.8 × 3.0~3.4 μm. The strain was confirmed based on phylogenetic analysis of the closest related organism, A. pseudotsugae CBS 288.37, using the partial 28S, internal transcribed spacer rDNA regions, and partial RNA polymerase II second largest subunit locus (RPB 2) gene sequences along with its culture characteristics. Therefore, morphological observations and phylogenetic analysis revealed that strain 17E029 is similar to the previously identified A. pseudotsugae. Hence, this species was described as A. pseudotsugae strain 17E029, which is a new record in Korea.

도열병균에서 추출한 Cx 효소의 순화 및 특성에 관한 연구

김상호,김은수 한국균학회 1982 韓國菌學會誌 Vol.10 No.2

The (NH₄)₂ SO₄, (70%) treated crude enzymes from the culture filtrates of the C-7^(+1) strain of Pyricularia oryzae which was grown on 2% CMC (carboxymethyl cellulose) for 8 days at 28℃, were chromatographied on Sephadex G-150 and DEAE-Sephadex A-25 columns. From the chromatography, three fractions of CMCase(C_x) was examined using Na-CMC as substrate. The C_x enzyme activity was optimal at pH 6.0 and 40℃, stable up to 40℃. The values of Km and Vmax of the enzyme were 2.8 × 10 mM and 5.9m moles/hour, respectively. The molecular weight determined by Sephadex G-150 column chromatography was around 80,000. Approximately sevenfold purified C_x enzyme gave a single protein band on the polyacrylamide gel electrophoresis.

First Report of Leptosphaerulina saccharicola Isolated from Persimmon (Diospyros kaki ) Tree Bark in Korea

( Okouma Nguia Fulbert ),( Benjamin Yaw Ayim ),( Kallol Das ),( Yang-sook Lim ),( Seung-yeol Lee ),( Hee-young Jung ) 한국균학회 2019 韓國菌學會誌 Vol.47 No.1

A fungal strain, designated PTT-2, was isolated from the bark of the trunk of a persimmon (Diospyros kaki) tree in Cheongdo, Korea. The isolate showed morphological similarities with Leptosphaerulina saccharicola. Strain PTT-2 had more rapid growth on potato dextrose agar medium than on oatmeal agar, malt extract agar, and synthetic nutrient poor agar media, with colony sizes of 53.8 mm, 49.8 mm, 48.4 mm, and 28.1 mm after 7 days at 25℃ temperature, respectively. Strain PTT-2 produced ascospores, which had irregular wavy edges, oblong to ellipsoidal shape, hyaline appearance and 23.6 × 10 μm size. The black ascomata were developed on PDA medium, and asci were recorded. A BLAST search of the internal transcribed spacer (ITS) region, TEF1-α and RPB2 gene sequences revealed that strain PTT-2 showed more than 99% nucleotide similarity with a strain of Leptosphaerulina saccharicola previously reported from Thailand. A neighbor-joining phylogenetic tree was constructed by concatenating the above-mentioned sequences, and showed that strain PTT-2 clustered in the same clade with L. saccharicola. Based on these findings, this is the first record of Leptosphaerulina saccharicola occurring in Korea.

One Unrecorded Endophytic Fungi from Sub-alpine Conifer, Rhizosphaera pini

( Ju-kyeong Eo ),( Eunsu Park ) 한국균학회 2019 韓國菌學會誌 Vol.47 No.2

An endophytic fungus, Rhizosphaera pini strain NIE7426, was isolated from the sub-alpine coniferous tree Abies nephrolepis in Mt. Nochu of Gangwon Province. It was characterized by macroscopic and microscopic features, as well as the internal transcribed spacer (ITS) 1, 2 and 5.8S sequences. All morphological and molecular features support the first recognition of this taxon in Korea. In addition, this study adds A. nephrolepis as a host plant R. pini.

A new record of Scleroconidioma sphagnicola isolated from soil in Korea

( Benjamin Yaw Ayim ),( Gihwan Sung ),( In-kyu Kang ),( Seung-yeol Lee ),( Hee-young Jung ) 한국균학회 2019 韓國菌學會誌 Vol.47 No.2

A fungal isolate, designated KNU-JJ-1824, was isolated from a soil sample collected from a field on Jeju, Korea. Colonies of the isolates cultured on PDA, MEA and CMAD for 14 days grew to diameters of 49~51 mm, 60~63 mm, and 47~50 mm, respectively. The colonies of this fungal isolate were dark green in color on both sides, and had irregular margins on the PDA media. Mycelia submerged and exhibited clear lines from the center to the edge on MEA media. On CMAD media, they were dark brown to greenish-brown, with narrow white margins. The shape of the conidia was guttulate, fusiform or clavate, and 1.9~3.4 × 6.5~10.2 μm in size. A molecular phylogenetic tree was constructed using dataset from small subunit (SSU) rDNA and the internal transcribed spacer (ITS) regions, and the isolate was found to cluster with Scleroconidioma sphagnicola UAMH 9731. Based on the results of the phylogenetic tree analysis and the cultural and morphological characteristics, the isolate was identified as Scleroconidioma sphagnicola. We report S. sphagnicola for the first time in Korea.

Acrophialophora ellipsoidea, an Undescribed Species Isolated from Soil in Korea

( Benjamin Yaw Ayim ),( Young-tae Kim ),( Kallol Das ),( In-kyu Kang ),( Seung-yeol Lee ),( Hee-young Jung ) 한국균학회 2019 韓國菌學會誌 Vol.47 No.3

A designated fungal isolate, KNU-US-1802E was isolated from the soil in Uiseong, Korea. To identify characteristics of the isolate, it was cultured on PDA media for 6 days at 35°C. Colonies on PDA are flat, light gray, dense, with entire margins; reverse dark gray to black, with white margins. Aerial mycelia were smooth-walled, hyaline and 40~42 mm diameter after 6 days at 35°C. Conidia were hyaline, one-celled, ellipsoidal to fusiform, forming long chains with average length x width of 5.0±0.3 × 2.9±0.2 μm. Molecular analysis indicates that the internal transcribed spacer (ITS) region and partial beta-tubulin (tub2) gene sequence showed 100% and 99% similarities, respectively with Acrophialophora ellipsoidea CGMCC 3.15255 collected from China. Phylogenetic analysis by the neighbor-joining (NJ) method shows that the KNU-US-1802E was clustered with A. ellipsoidea CGMCC 3.15255 in a phylogenetic tree constructed using the concatenated sequences of ITS region and tub2 gene sequences with a high bootstrap value. Based on these findings, the isolate KNU-US-1802E was identified as Acrophialophora ellipsoidea, and this is the first report of this isolate in Korea.