競走馬에서의 藥物檢査

金尙塡 대한수의학회 1990 대한수의학회 학술대회발표집 Vol.1990 No.-

Salmonella pullorum lipopolysaccharide의 acridine organe에 의한 O-side chain 길이의 변화

김종배,김태우,이원용,양세환,Kim, Jong-bae,Kim, Tae-ue,Lee, Won-yong,Yang, Se-whan 대한수의학회 1993 大韓獸醫學會誌 Vol.33 No.1

The morphological hetergeneity of lipopolysaccharides(LPSs) and variation in the O-side chain lengths of LPSs of Salmonella pullorum, which was serially subcultured on the brain heart infusion agar containing $100{\mu}g/m{\ell}$ of acridine orange, was analyzed in Sephadex G-50 column chromatography and silver-stained polyacrylamide gels. The biochemical differences in LPS W and LPS A0150 were identified. Increases in the contents of O-polysaccharides of LPS A0150 than those of LPS W were reflected in the profiles of chromatography and silver-stained polyacrylamide gels. In summary, LPS molecules of S pullorum A0150 appeared to be enriched in the molecules with long O-polysaccharide chains than those of LPS W.

오리의 Pasteurella anatipestifer 감염증 발생

최정옥,김경년,Choi, Chung-ok,Kim, Gyoung-nyoun 대한수의학회 1993 大韓獸醫學會誌 Vol.33 No.1

This study was conducted to investigate the cause of a new duck disease occured in southern part of Korea. A meat type duck farm located in Kangjin, Chonnam Province had experienced outbreaks of septicemic disease at around 20 days of age in nearly every batch of ducklings from early spring to early summer in 1989. Main symptoms of the birds were eye and nasal discharge, depression, inappetence, diarrhea and nervous signs such as tremor and ataxia. Some birds died suddenly without any signs. Mortality reached from 20% to 80% in severe cases. The autopsy findings of the affected ducklings revealed consistantly severe airsacculitis, fibropericarditis, perihepatitis and occasionaly enteritis and distended ureter with urate deposit. A rod shaped gram-negative bacterium was isolated purely from brain and liver of the diseased ducks by culturing the specimens on blood agar for 48 hours in candle jar. The isolate neither produced hemolysis nor grew on MacConKey Agar. It formed colony relatively slowly being recognizable at least 36 hours after culturing, reaching colony size of about 1mm in diameter at 48 hours culture. The colony looked iridescent under oblique light and had muddy odor. The isolate did not ferment carbohydrates tested but produced gelatinase, hippuricase and oxidase which were considered as characteristics of P anatipestifer. The isolate induced similar signs and lesions when infected experimentally into ducks of 3 to 38 days age via intraperitoneum or intratrachea. However it did not produce any clinical signs wen inoculated via intranasal route. It produced only mild signs in chicken just injected with a very large dose. The bacteria did not produce any signas or lesions in mice. It was concluded through biochemical and physiological tests and animal inoculation tests that the new disease was caused by P anatipestifer.

Babesia gibsoni 항원접종과 Theileria sergenti를 비특이 항원으로 접종한 개의 면역효과에 관한 연구

윤창모,이주묵,채준석,권오덕,Yoon, Chang-mo,Lee, Joo-mook,Chae, Joon-seok,Kwon, Oh-deog 대한수의학회 1993 大韓獸醫學會誌 Vol.33 No.1

To examine the effects of vaccination against Babesia gibsoni(B gibsoni) infection in dogs, 15 normal mixed-breed dogs(5 months to 1 year old) were divided into 3 groups with 5 dogs in each group. One of them was selected as control group(group A) and other were selected as experimental groups(group B and C). The group B was vaccinated with antigen which were mixed 0.2% of formalin treated B gibsoni and sonicated one. The group C was inoculated Theileria sergenti as a non-specific antigen. The results obtained in this experiment were summarized as follows; 1. After first vaccination, antibody titers of group B and C were increased 5 times(1:200) than those of control group(1 : 40). The antibody titers of group C were increased more than that of group B after second vaccination. When challenged with the living protozoa(Babesia gibsoni), the antibody titers of C group were elevated higher than that of B group and maintained steadly. Those were not exceeded over 1 : 5,000 for 4 weeks in all 3 groups. 2. After challenge, the peak time of the parasitemia appeared nearly on the 15th day(12~18 days) in all groups. During this period, the rate of parasitized erythrocytes in control group was $55.0{\pm}5.4$‰. But that of group B and C were $41.3{\pm}38.8$‰ and $15.2{\pm}16.3$‰, respectively. 3. After challenge with B gibsoni, all of the values of PCV, Hb, RBC and total leukocytes counts were decreased in both of the experimental and the control. 4. In all groups, there were increased lymphocytes and monocytes after challenge with the protozoa.

제주마(濟州馬)의 위장관(胃腸管)에 기생(寄生)하는 Gasterophilus spp의 분류(分類) 및 분포조사(分布調査)

김승호,Kim, Seung-ho 대한수의학회 1993 大韓獸醫學會誌 Vol.33 No.1

This paper dealt with the situation and the hatch rate of bot's eggs on the equine hairs in Cheju horse with the species grouping of the bot flies of equine(genus Gasterophilus). The prevalence and infection dynamics of Gasterophilus spp. larvae was also evaluated in the equine alimentary canal submitted in this laboratory for the necropsy or from the abattoir. Samples including Gasterophilus spp. larvae, bot's flies and its eggs, which were collected from the alimentary canal and equine hairs, respectively, were studied and classified by morphology. The morphologic feature of the spines of Gasterophilus larvae were studied by scanning electron microscope. 1. Gasterophilus intestinalis larvae concentrated in the nonglandular portions of the stomach. The infection of second-, and third stage larvae were common in November, and from January to October, respectively. Gasterophilus nasalis larvae were commonly identified on the gastric pylorus and upper portion of duodenum. Second stage larvae were found from October to December, and 3rd stage larvae, from January to September. 2. The hatch rate of laid eggs of Gasterophilus intestinalis was 28.4%, and that of Gasterophilus nasalis was 79.5%. The hatch rate of Gasterophilus intestinalis eggs was highest(62.5%) in December. The hatch rate of laid eggs were higher in the region of scapula(64.0%) and limbs(62.5%) than on the maned hairs. The eggs of Gasterophilus nasalis were completely hatched by October. 3. Eight hundred five Cheju horses examined in this study were infected with the eggs of Gasterophilus spp. Gasterophilus intestinalis eggs on the body regions from sixty horses were recognized in phalangeal (14.4%), in abdominal(13.8%), metacarpal, brachial and cervical regions. Gasterophilus nasalis eggs were uncommon and recognized in submandibular regions(1.4%). 4. In conclusion, the infection of imago, larvae and eggs of both Gasterophilus intestinalis and Gasterophilus nasalis were indentified in cheju horse.

소 결핵균의 면역세포화학적 동정

김순복,서정향,문운경,Kim, Soon-bok,Sur, Jung-hyang,Moon, Oun-gyeong 대한수의학회 1993 大韓獸醫學會誌 Vol.33 No.1

The present study was intended to use the avidin-biotin-peroxidase-antiperoxidase complex (ABPAP) method for the identification of Mycobacterium bovis in the tissue sections of infected cattle. Antibodies and linksera for ABPAP procedure used in incubated order were rabbit anti-Mycobacterium polyvalent antibodies, goat anti-rabbit IgG, rabbit peroxidase-antiperoxidase complex, biotinyl-horse anti-rabbit IgG, and avidin-biotin-peroxidase complex. Where the bacterial antigen was localized by ABPAP, a dark brown deposit occurred in the cytoplasms of macrophages and Langerhans' giant cells of the granulomatous lesions. The method approved to be highly specific for the identification of the bacteria and allowed a precise localization of the bacterial antigen in infected cells.

토끼의 바이러스성 간염(肝炎)에 관한 연구(硏究) II. 실험적(實驗的) 오염(汚染) 토끼 비장(脾臟)의 전자현미경적(電子顯微鏡的) 관찰(觀察)

이차수,권영란,정종식,신태균,Lee, Cha-soo,Kwon, Young-ran,Jyeong, Jong-sik,Shin, Tae-kyun 대한수의학회 1993 大韓獸醫學會誌 Vol.33 No.1

An acute fatal infectious disease in rabbits has been outbroken in Korea since 1985. This disease has been characterized as an acute hepatitis caused by viruses. However, viral pathogenesis in rabbit viral hepatitis leading to sudden death remain unclear. This report dealt with the electron microscopic findings on the spleen of experimentally infected rabbits, because spleen is one of the affected organs which have high titer of virus by a haemagglutination test. A typical crystalline array of virus was not found in the splenic cells of infected rabbits with acute hepatitis. Virus-like particles were seen within the phagosome of macrophages of the spleen. Ultrastructural changes in the spleen were severe with the lapse of time after inoculation. From these results, virus-like particles in the spleen were supposed to be phagocytosed by macrophage during viremia, while active replication of virus occurred in the liver. It was concluded that sudden death in this viral disease was caused by hepatic coma and/or circulatory disturbance.

축우 부루셀라병의 ELISA 진단법에 관한 연구

임윤규,이두식,박전홍,양기천,김승호,김공식,현관종,김우택,이영순,Lim, Yoon-kyu,Lee, Doo-sick,Park, Jun-hong,Yang, Ki-chun,Kim, Seung-ho,Kim, Kong-sick,Hyun, Kwan-jong,Kim, Woo-tack,Lee, Yong-soon 대한수의학회 1993 大韓獸醫學會誌 Vol.33 No.1

Enzyme-linked Immuno sorbent Assay (ELISA) for the serological diagnosis of Brucella abortus was developed and compared with plate agglutination test. Cell wall antigen was extracted from Brucella abortus 1119-3 by sonication and with a sodium deoxychlate solution. Optimum protein concentration of coating antigen were $0.4{\mu}g/100{\mu}{\ell}$ protein on each microtiter plate well. Horse radish peroxidase (HRP) labeled protein-G was used as a tracer of reacted antibodies. ELISA confirmed the agreeable results of 40 cases out of 43 cases by plate aggulutination test. ELISA diagnosed positive cases(10 out of 12) and negative cases (1 out of 12) with dubious sera by plate agglutination test. From this results ELISA could be used for the early diagnostic tools of Brucellosis in cattle.

리포좀 피포성 buparvaquone의 Theileria sergenti 인공감염 송아지에 대한 치료효과

김두,Kim, Doo 대한수의학회 1993 大韓獸醫學會誌 Vol.33 No.1

This study was carried out to completelycure the experimental bovine theileriasis with small unilamella vesicle liposome incorporated buparvaquone which was effective both to schizonts in lymphocyte of lymph nodes and piroplasmic stage in erythrocytes. Small unilamella vesicle liposome incorporated buparvaquone was prepared by French pressure cell method using egg phosphatidylcholine. The diameter of the vesicles was ranged from 5 to 220 nm, but the most vesicles were ranged from 10 to 50 nm in diameter. The incorporation rate for buparvaquone was 100%. Parasitaemia of the 10 calves inoculated with $5{\times}10^8$ erythrocytes infected with Theileria sergenti were first detected from on day 16 to day 23 after inoculation. In calves treated with a dose rate 2.5 mg/kg BW of free buparvaquone, a gradual decrease in piroplasmic parasitaemia was observed following treatment to day 5. However parasitaemia levels returned to near pretreatment values after approximately 60 days. In calves treated with a dose rate 5.0mg/kg BW of free buparvaquone, parasitaemia were disappeared on day 3 after treatment, but there was a mild recrudescence of infection on day 28 after treatment. In calves treated intraavenously with a dose rate 2.5 mg/kg BW of buparvaquone incorporated in liposome, the calves were all cured on day 2 or day 3 after treatment. In calves treated subcutaneously and intraperiotoneally with a dose rate 2.5 mg/kg BW of buparvaquone incorporated in liposome, parasitaemia were disappeared on day 3 or day 4 after treatment, but there was a mild recrudescence of infection on day 40 or day 45 after treatment.

기니픽 심근의 전기생리학적 특성에 미치는 α¹-Adrenoceptor 자극효과

김진상,Kim, Jin-sang 대한수의학회 1993 大韓獸醫學會誌 Vol.33 No.2

The effects of ${\alpha}_1$-adrenergic stimulation on membrane potential, intracellular sodium activity $(a_N{^i{_a}})$, and contractility were investigated in the isolated papillary muscle of euthyroid, hyperthyroid, and hypothyroid guinea pigs. Cardiac alterations in the thyroid state have been shown to induce marked changes in action potential characteristics, the most pronounced shortening of action potential duration by hyperthyroidism and an increase in duration by hypothyroidism. $10^{-5}M$ Phenylephrine produced a decrease in $(a_N{^i{_a}})$ in euthyroid and hypothyroid preparations, but an increase in $(a_N{^i{_a}})$ in hyperthyroid ones. The major findings were that phenylephrine produced a stronger positive inotropic effect(PIE) without initial negative inotropic effect(NIE) in hyperthyroid preparations, while phenylephrine produced markedly NIE in hypothyroid ones. The alterations in membrane potential, $(a_N{^i{_a}})$, and contractility were abolished by $3{\times}10^{-5}M$ prazosin in hypothyroidism. In hypothyroid ventricular muscle, the decrease in $(a_N{^i{_a}})$ caused by phenylephrine were not abolished or reduced by $10^{-5}M$ strophanthidin, $10^{-5}M$ TTX, $3{\times}10^{-4}M$ lidocaine, or $100^{-5}M$ verapamil. These results indicate that the ${\alpha}_1$-adrenoceptor-mediated decrease in $(a_N{^i{_a}})$ is not associated with a stimulation of the $Na^+$-$K^+$ pump, inhibition of the $Na^+$ or $Ca^+$ channel in hypothyroid ventricular muscle. $10^{-5}M$ Phenylephrine decreased $(a_N{^i{_a}})$ but increased $(a_N{^i{_a}})$ in the presence of a PKC activator phorbol dibutyrate$(PDB_u)$. In conclusion, it is suggested that the following sequence of events in response to phenyleplhane occur in guinea pig ventricular muscle. First, changes in thyroid state may contribute to the ventacular electrophysiological propeties or ion transport system. Second, the adrenoceptor-mediated initial transient NIE may be associated with the decrease in $(a_N{^i{_a}})$ by PKC activation.