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Effects of naringin on fibrosis in a rats model with polycystic ovary syndrome (PCOS)
Zhewen Dong,Huan Lan,Wanying Li,Qi Qi Pang,Jia- Le Song,Yanyuan Zhou 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
To observe the effect of naringin intervention and its ameliorating effect on ovarian fibrosis in letrozole induced POCS rats. SD rats were divided into normal, PCOS and naringin group. The body weight(BW) was recorded every 3 days. ELISA kits were used to measure serum levels of FSH, LH, E2, and T in PCOS rats. The levels of CollagenI, CTGF, α-SMA were detected by Western Blotting assay. The BW of PCOS rats were significantly increased than that in control group (P<0.05). Naringin treatment reduced the BW in PCOS rats. Compared with normal group, the serum level of E2 in PCOS group was decreased, and the serum T, LH, FSH, LH/FSH ratio levels were increased. However, administrated with naringin increased the serum E2 levels, and also decreased the serum levels of T, LH, FSH, LH/ FSH levels in PCOS rats. The protein levels of CollagenI, CTGF and α-SMA were increased in varian of PCOS rats than that in normal group. Naringin treatment was able to decrease the protein levels of CollagenI, CTGF and α-SMA in varian of PCOS rats.The results showed that naringin intervention could improve the metabolic imbalance and the occurrence of fibrosis in PCOS rats.
Wanying Li,Yanmin Su,Zixian Wang,Zhewen Dong,Haokun Chen,Shumei Zhong,Qi Qi Pang,Jia-Le Song 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
To observe the effect of SA on DSS-induced intestinal fibrosis in mice. The mice were randomly divided into normal, DSS and SA treated DSS mice group. Changes in body weight(BW), blood stool and diarrhea were observed. The serum levels of IL-1β, IL-6, IL-17A, IL-18 and TNF-α were determined by ELISA kits. The protein levels of NLRP3, caspase-1, ASC, IL-1 β, α-SMA, CollagenI, Beclin1, LC3II/I and p62 were detected by Western Blotting assay. Compared with the normal group, SA significantly inhibited the loss of BW and DAI score in colitis mice (P<0.05). H&E and Masson staining assay suggested the SA reduced epithelial cell damage, crypt structure loss, inflammatory cell infiltration, and the fibrogenesis in colon of colitis mice. The serum levels of IL-1β, IL-6, IL-17A, IL-18 and TNF-α were decreased by SA(P<0.05). SA was able to reduce the protein levels of NLRP3, Caspase-1, ASC, IL-1β, α-SMA, Collagen-I, p62, and increased the expressions of Beclin1 and LC3II/I in colitis mice. The study demonstrated that SA can modulate the activity of NLRP3 inflammasome and autophagy pathway to improve DSS induced intaestinal fibrosis in mice.
Protective activities of probiotics in letrozole-induced polycystic ovarian syndrome (PCOS) rats
Xinyu Li,Chenyang Xie,Qiuping Huang,Eun Mi Hwang,Bin Jin,Zhewen Dong,Zhen Zeng,Wen He,Qi Qi Pang,Jia-Le Song 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
To investigate the effect of Bifidobacterium, C. butyricum and L. plantarum on the letrozole induced PCOS rats. All female SD rats were randomly divided into five groups, modeling PCOS rats, and respectively given intragastric administration of Bifidobacterium, C. butyricum, and L. plantarum solution (1×10<SUP>9</SUP> CFU/mL) for 30 days, and observed body weight (BW). The serum levels of testosterone (T), LH, E2, FSH, INS, TG, TC, LDL, HDL were determined by ELISA kits. The levels of TGF-β1, smad4, smad3, p-smad3, p-smad2, smad2 were detected by Western Blotting. Compared with normal group, the BW, T, LH, INS, TG, TC LDL-C in PCOS rats were significantly increased, and the levels of E2, FSH and HDL-C were significantly decreased. Administrated with different probiotics can decrease the BW, T, LH, INS, TG, TC,LDL-C, and the levels of E2, FSH and HDL-C increased in PCOS rats. All probiotics significantly decreased the expression of p-smad2, p-smad3, smad4, TGF-β1. Our results showed that Bifidobacterium, C. butyricum and L. plantarum were all effective in regulating hormone levels, improving IR and dyslipidemia, and improving ovarian fibrosis in PCOS rats.
Huan Lan,Zhewen Dong,Yaqi Wu,Chaojie Chong,Zhiqiang Liu,Wanying Li,Zixian Wang,Junyang Liu,Qi Qi Pang,Jia-Le Song,Yanyuan Zhou 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
In this study, we investigated the antifibrotic effects of Rha on letrozole-induced PCOS in rats. Rha treatment restored letrozole-induced alterations in the body weight (BW), relative weights of the ovaries, and relative weights of uterine and visceral adipose tissues. Histological observation showed that Rha reduced the number of atretic follicles, cystic follicles, and fibrosis of the ovaries in the PCOS rats. Administration of Rha reduced letrozole-induced metabolic dysfunction by decreasing the serum levels of TG and TC and increasing the levels of HDL-C in the PCOS rats. Rha treatment also modulated the serum levels of sex hormones, which decreased the LH, E2 and T and increased the FSH in PCOS rats. Finally, Rha treatment appeared to increase the activity of PPAR-γ, reduce the activation of the TGF-β1/Smad pathway, and reduce the collagen I, α-SMA and CTGF levels in the ovaries of the PCOS rats. Our results demonstrated that Rha significantly ameliorated metabolic disturbances and ovarian fibrosis in the letrozole-induced PCOS rats. Rha may be an effective compound in preventing ovarian fibrosis in the future.
Huan Lan,Zhewen Dong,Yaqi Wu,Chaojie Chong,Zhiqiang Liu,Wanying Li,Zixian Wang,Junyang Liu,Qi Qi Pang,Jia-Le Song,Yanyuan Zhou 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
This study investigated the antifibrotic effects of SA on ovarian fibrosis in letrozole-induced PCOS in rats. SA treatment restored letrozole-induced alterations in the body weight (BW), relative weights of the ovaries, and visceral adipose tissues. Histological observation showed that SA reduced the number of atretic follicles, cystic follicles, and fibrosis of the ovaries in PCOS rats. SA treatment also modulated the serum levels of sex hormones, which decreased the luteinizing hormone (LH), and testosterone (T) and increased the follicle-stimulating hormone (FSH) in PCOS rats. Administration of SA reduced letrozole-induced metabolic dysfunction by decreasing the serum levels of triglyceride (TG) and total cholesterol (TC) and increasing the levels of high-density lipoprotein cholesterol (HDL-C) in PCOS rats. Finally, SA treatment appeared to increase the activity of PPAR-γ, reduce the activation of the TGF-β1/Smad pathway, and reduce the collagen I, α-SMA and CTGF levels in the ovaries of the PCOS rats. Our results demonstrated that SA significantly ameliorated ovarian fibrosis and abolic disturbances in the letrozole-induced PCOS rats.
Zhiqiang Liu,Chaojie Chong,Zhewen Dong,Yaqi Wu,Wanying Li,Haokun Chen,Shumei Zhong,Junyang Liu,Qi Qi Pang,Jia-Le Song,Yanyuan Zhou 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
To investigate the effect of ACP on letrozole-induced PCOS rats. All rats were divided into normal groups, PCOS group, metformin group and ACP group. The body weight (BW) was recorded every three days, and the final body weight as well as ovarian weight were recorded. Serum levels of TC, TG, HDL-C, LDL-C and E2, T, FSH and LH were measured by ELISA assay, and the LH/FSH ratio was calculated. The results showed that ACP decreased the BW and the ratio of ovarian to BW. Compared with the normal group, the serum levels of T, LH, LH/FSH, TG, TC, LDL-C increased, and the levels of FSH, E2, HDL-C decreased in PCOS group. ACP effectively improves the serum levels of FSH, LH/FSH, TG, TC, LDL-C and other related factors. The results of H&E staining of ovarian tissues showed that the ACP group showed different degrees of improvement in the reduced number of corpus luteum, thinning of granulosa cell layer, follicular capsular dilatation and follicular atresia. ACP not only reduces BW and the ratio of ovarian to BW, but also it can elevate the level of FSH and reduce LH/FSH, TG, TC and HDL-C levels. Furthermore, ACP can protect ovarian tissue to some extent.
Zixian Wang,Wanying Li,Zhewen Dong,Chaojie Chong,Yaqi Wu,Shumei Zhong,Haokun Chen,Qi Qi Pang,Jia-Le Song 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
To observe the effect of cyanidin-3,5-O-diglucoside(C3G) on intestinal fibrosis in a model of dextran sulphate sodium (DSS)-induced mice with colitis. Colitis was induced in C57BL/6 mice by addition of DSS to drinking water. Clinical (body weight, colon length and colon weight) and histologic changes were evaluated. In addition, colon levels of interleukin-1β, IL-6, IL-18, IL-17A, tumor necrosis factor(TNF)-α were detected by enzyme-linked immunosorbent assay. Relative to the DSS group, the mice in the C3G treatment group increased in body weight, colon length, and the ratio of colon weight to length was significantly lower (P<0.05). The DAI index of mice in the intervention group was lower than that in the colitis model group (P<0.05). Histological observation also found that the intervention of C3G is able to reduce the infiltration of inflammatory cells in colon tissue induced by DSS. The results of Masson staining suggest that the intervention of C3G also reduces the occurrence of intestinal fibrosis in mice with colitis. Our results suggested that C3G is an activity to improve inflammation and intestinal fibrosis in DSS colitis mice.
Shumei Zhong,Wanying Li,Zixian Wang,Haokun Chen,Yaqi Wu,Chaojie Chong,Zhewen Dong,Jia-Le Song 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
To investigate the effects of AMP on letrozole-induced PCOS rats. All SD rats were randomly divided into normal, PCOS, metformin group (Met, 265 mg/kg) and AMP group (1 g/kg) and weighed every three days. After 21 days of continuous administration, oral glucose tolerance test was performed, and the blood glucose(BP) level was measured. After execution, calculate the relative weight of bilateral ovaries. Serum levels of FSH, LH, E2, T and FINS were detected by ELISA assay. Serum levels of TC, TG, HDL-C and LDL-C were detected by the kit and calculated the LH/FSH ratio and HOMA-IR level. Compared with the normal group, body weight and relative weight of ovaries, BP level and serum levels of T, LH, LH/FSH ratio, FINS, HOMA-IR, TC, TG, LDL-C, and E2, FSH, HDL-C in model group increased. Compared with the PCOS group, body weight in the Met group and AMP group was lower, BP level and serum levels of T, LH, FINS, HOMA-IR and LDL-C in the Met group and AMP group were significantly lower and the level of HDL-C was higher. Armillaria mellea polysaccharides can improve hormone disturbance, glucose metabolism and abnormal lipid level in letrozole-induced polycystic ovary syndrome model rats.
Hailiang Yang,Xiao Wang,Peng Ni,Zhewen Li,Huixia Liu 대한금속·재료학회 2022 METALS AND MATERIALS International Vol.28 No.8
In order to use numerical simulation to reveal the formability of magnesium–lithium alloys and other light alloys under highstrain rate, it is crucial to construct a constitutive model and a forming limit diagram (FLD) of LA103Z alloy that was formedby laser shock at high strain rate. In this study, three different uniaxial tensile experiments on the ultralight alloy materialLA103Z magnesium alloy were conducted, the mechanical behavior of the material with sensitivity to high strain rate wasstudied, and a Johnson–Cook (JC) constitutive model sensitive to high strain rates and suitable for laser shock forming wasproposed. At the same time, the constitutive parameters and failure parameters of the modified JC model of LA103Z magnesiumalloy were obtained through three different uniaxial tensile experiments. On the basis of the relationship betweentensile loading and stress–strain of LA103Z magnesium alloy, the experiments demonstrated the precision of the modified JCconstitutive model. A modified constitutive model was then developed by using the user material subroutine VUMAT, andthen it was applied to ABAQUS. Finally, the formability of the material was simulated, and the FLD of LA103Z magnesiumalloy material in laser shock forming was predicted. This study provides guidance on the actual production and applicationof LA103Z magnesium alloys.
Junyang Liu,Zixian Wang,Wanying Li,Zhewen Dong,Chaojie Chong,Yaqi Wu,Shumei Zhong,Haokun Chen,Zhiqiang Liu,Qi Qi Pang,Jia-Le Song 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
To explore the effect of C3G on intestinal mucosal injury in 5-FU induced BALB/c mice. Mice were randomly divided into normal, model and C3G groups. After the last administration, mice were anesthetized, and calculated the thymus and spleen indexes. The ileum was used to stain with H&E observation. Serum levels of IL-1β, IL-6, IL-10 and TNF-α were determined by ELISA kits. Compared with the normal group, the model group had diarrhea, body weight and thymus and spleen index decreased significantly, intestinal villus epithelial cells fell off, crypt structure was destroyed, and inflammatory cell infiltration was observed. The body weight, thymus and spleen indexes of the treatment group and the prevention group decreased less, and the levels of TNF-α, IL-1β and IL-6 were significantly decreased, and the level of IL-10 was significantly increased relative to the model group. The intestinal villi structure was relatively complete, and a small amount of crypt structure was destroyed, with a small amount of inflammatory cell infiltration, which reduced the mucosal damage. The administration of C3G could protect intestinal mucosa from 5-FU-induced injury.