Transcriptome sequencing reveals non-coding RNAs respond to porcine reproductive and respiratory syndrome virus and Haemophilus parasuis co-infection in Kele piglets

( Jing Zhang ),( Chunping Zhao ),( Min Yao ),( Jing Qi ),( Ya Tan ),( Kaizhi Shi ),( Jing Wang ),( Sixuan Zhou ),( Zhixin Li ) 한국축산학회 2024 한국축산학회지 Vol.66 No.4

Co-infection with porcine reproductive and respiratory syndrome virus (PRRSV) and Haemophilus parasuis (HPS) has severely restricted the healthy development of pig breeding. Exploring disease resistance of non-coding RNAs in pigs co-infected with PRRSV and HPS is therefore critical to complement and elucidate the molecular mechanisms of disease resistance in Kele piglets and to innovate the use of local pig germplasm resources in China. RNA-seq of lungs from Kele piglets with single-infection of PRRSV or HPS and co-infection of both pathogens was performed. Two hundred and twenty-five differentially expressed long non-coding RNAs (DElncRNAs) and 30 DEmicroRNAs (DEmiRNAs) were identified and characterized in the PRRSV and HPS co-infection (PRRSV-HPS) group. Compared with the single-infection groups, 146 unique DElncRNAs, 17 unique DEmiRNAs, and 206 target differentially expressed genes (DEGs) were identified in the PRRSV-HPS group. The expression patterns of 20 DEmiRNAs and DElncRNAs confirmed by real-time quantitative polymerase chain reaction (RT-qPCR) were consistent with those determined by high-throughput sequencing. In the PRRSV-HPS group, the target DEGs were enriched in eight immune Gene Ontology terms relating to two unique DEmiRNAs and 16 DElncRNAs, and the unique target DEGs participated the host immune response to pathogens infection by affecting 15 immune-related Kyoto Encyclopedia of Genes and Genomes enrichment pathways. Notably, competitive endogenous RNA (ceRNA) networks of different groups were constructed, and the ssc-miR-671-5p miRNA was validated as a potential regulatory factor to regulate DTX4 and AEBP1 genes to achieve innate antiviral effects and inhibit pulmonary fibrosis by dual-luciferase reporter assays. These results provided insight into further study on the molecular mechanisms of resistance to PRRSV and HPS co-infection in Kele piglets.

Sphingolipid composition and metabolism differ in three auchenorrhynchous pests of rice

Zhang Min-Jing,Shi Xiao-Xiao,Bai Yue-Liang,Zhou Wen-Wu,Zhu Zeng-Rong 한국응용곤충학회 2021 Journal of Asia-Pacific Entomology Vol.24 No.3

Sphingolipids (SPLs), a group of membrane and intracellular lipids, mediate numerous cellular processes. The composition and metabolism of sphingolipids varies according to species and the sphingolipid studies of insects, as compared to mammals, are not yet clear. In the current study, we subjected three auchenorrhynchous insects, including whitebacked planthopper (WBPH), Sogatella furcifera, small brown planthopper (SBPH), Laodelphax striatellus, and green rice leafhopper (GRLH), Nephotettix cincticeps, as representative rice pests for sphingolipid analysis. We performed sphingolipid species profiling using UPLC-Q-TOF-MS/MS and isolated sphingolipid metabolic genes from their transcriptomic data. C14-, C16-, C18- and C20-sphingoid base were detected in both planthoppers, but no C14- sphingoid base was found in GRLH. The planthoppers had more abundant sphingosine-1-phosphates (Sph-1-P) and ceramides than leafhopper. A total of 14, 13 and 16 sphingomyelin species were found in SBPH, WBPH and GRLH, respectively. The composition of sphingomyelin species varied in three insects. Coordinated with the abundance in sphingomyelin species, the leafhopper possessed more sphin gomyelinase (SMase) gene isoforms for metabolism of different sphingomyelins than planthoppers. The phylo genetic analysis showed the three tested insects all possessed one potential neutral-SMase homologue, whereas SBPH and GRLH both had another potential acid-SMase homologue. This study is a comprehensive sphingoli pidomic analysis, suggesting that sphingolipid profiles significantly differed among the three insects. By providing information of sphingolipid metabolic gene homologues of these three insects, our findings will contribute to the further sphingolipid studies of auchenorrhynchous insects and provide a research foundation for rice pest management.

HIIT 프로그램 적용이 비만 여중생의 신체구성과 체력에 미치는 효과

장징이(Zhang, Jing Yi),김창영(Kim, Chang Young),이민기(Lee, Min Ki),안민지(An, Min Ji) 학습자중심교과교육학회 2018 학습자중심교과교육연구 Vol.18 No.11

이 연구는 8주 동안의 HIIT(고강도 인터벌 트레이닝) 프로그램의 적용이 비만 여자 중학생의 신체구성과 체력에 미치는 효과를 분석하여, 학교 현장에서 청소년 비만을 예방하고 관리하는데 필요한 기초 자료를 제공하고자 한다. 연구 목적을 달성하기 위하여 충북 C시에 소재한 K중학교 2학년 여학생 24명을 임의로 선정한 후, 운동집단과 통제집단에 각각 12명씩 무선 배정하고 고강도 인터벌 트레이닝 전ㆍ후 신체구성과 체력을 측정하였다. 고강도 인터벌 트레이닝은 주3회 20분씩, 8주간 실시하였으며, t검증을 이용해 집단 내, 집단 간 차이를 분석하였으며, 연구 결과는 다음과 같다. 첫째, 실험 후 운동집단의 체중과 체지방률은 유의하게 감소하였고, 체지방 체중에는 차이가 나타나지 않았다. 둘째, 실험 후 운동집단의 체지방률이 통제집단에 비해 통계적으로 유의하게 감소하였다. 셋째, 실험 후 운동집단의 심폐지구력과 근지구력이 유의한 차이로 향상되었으며, 근력, 유연성, 순발력에서는 차이가 나타나지 않았다. 넷째, 운동집단의 심폐지구력이 통제집단에 비해 통계적으로 유의하게 향상되었다. The aim of this study was to investigate the influence of the exercise program that applied the 8 weeks of HIIT method on the body composition and physical fitness in obese middle school students. In order to accomplish the aim of this study, 24 2nd grade female students at K middle school located in Cheongju city, Chungbuk-do, were selected at random and the allocated 12 students each for the exercise group and control group and measured body composition and physical fitness before and after the HIIT. HIIT program is implemented for twenty minutes of three times each week for 8 weeks and the t-test was used to analyze the difference in the group and between the groups. Looking into the changes of body composition before and after the exercise group that participated regularly to the 8-week HIIT program, the weight and % body fat were noticeably reduced after the experiment and there was no difference in the fat free mass. Looking into the difference of exercise group and control group, the % bofy fat of the exercise group after the experiment was noticeably reduced statistically compared to the control group. Looking into the changes of physical fitness before and after the exercise group that participated regularly to the 8-week HIIT program, the cardiorespiratory endurance and muscular endurance of the exercise group after the experiment was noticeably improved and there was no difference with respect to the muscle strength, flexibility, and agility. Looking into the difference of exercise group and control group, the cardiorespiratory endurance of the exercise group after the experiment was noticeably improved statistically compared to the control group.

Association of CYP2E1 and NAT2 Polymorphisms with Lung Cancer Susceptibility among Mongolian and Han Populations in the Inner Mongolian Region

Zhang, Jing-Wen,Yu, Wan-Jia,Sheng, Xiao-Min,Chang, Fu-Hou,Bai, Tu-Ya,Lv, Xiao-Li,Wang, Guang,Liu, Su-Zhen Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.21

Purpose: To explore associations of CYP2E1 and NAT2 polymorphisms with lung cancer susceptibility among Mongolian and Han populations in the Inner Mongolian region. Materials and Methods: CYP2E1 and NAT2 polymorphisms were detected by PCR-RFLP in 930 lung cancer patients and 1000 controls. Results: (1) Disequilibrium of the distribution of NAT2 polymorphism was found in lung cancer patients among Han and Mongolian populations (p=0.031). (2) Lung cancer risk was higher in individuals with c1, D allele of CYP2E1 RsaI/PstI, DraI polymorphisms and slow acetylation of NAT2 (c1 compared with c2, OR=1.382, 95%CI: 1.178-1.587, p=0.003; D compared with C, OR=1.241, 95%CI: 1.053-1.419, P<0.001; slow acetylation compared with rapid acetylation, OR=1.359, 95%CI:1.042-1.768, p=0.056) (3) Compared with c2/c2 and rapid acetylation, c1/c1 together with slow acetylation synergetically increased risk of lung cancer 2.83 fold. (4) Smokers with CYP2E1 c1/c1, DD, and NAT2 slow acetylation have 2.365, 1.916, 1.841 fold lung cancer risk than others with c2/c2, CC and NAT2 rapid acetylation, respectively. (5) Han smokers with NAT2 slow acetylation have 1.974 fold lung cancer risk than others with rapid acetylation. Conclusions: Disequilibrium distribution of NAT2 polymorphism was found in lung cancer patients among Han and Mongolian populations. Besides, Han smokers with NAT2 slow acetylation may have higher lung cancer risk compared with rapid acetylation couterparts. CYP2E1 c1/c1, DD and NAT2 slow acetylation, especially combined with smoking, contributes to the development of lung cancer. CYP2E1 c1/c1 or DD genotype and NAT2 slow acetylation have strong synergistic action in increasing lung cancer risk.

Rhapontigenin from Rheum undulatum Protects Against Oxidative-Stress-Induced Cell Damage Through Antioxidant Activity

Zhang, Rui,Kang, Kyoung Ah,Piao, Mei Jing,Lee, Kyoung Hwa,Jang, Hye Suk,Park, Min Jeong,Kim, Bum Joon,Kim, Jin Sook,Kim, Young Sup,Ryu, Shi Yong,Hyun, Jin Won Taylor Francis 2007 Journal of toxicology and environmental health. Pa Vol.70 No.13

<P> The antioxidant properties of rhapontigenin and rhaponticin isolated from Rheum undulatum were investigated. Rhapontigenin was found to scavenge intracellular reactive oxygen species (ROS), the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, and hydrogen peroxide (H2O2). The radical scavenging effect of rhapontigenin was more effective than rhaponticin. Rhapontigenin protected against H2O2-induced membrane lipid peroxidation and cellular DNA damage, which are the main targets of oxidative stress-induced cellular damage. The radical scavenging activity of rhapontigenin protected Chinese hamster lung fibroblast (V79-4) cells exposed to H2O2 by inhibiting apoptosis. Rhapontigenin inhibited cell damage induced by serum starvation and was also found to increase the activity of catalase and its protein expression. Further, rhapontigenin increased phosphorylation of extracellular signal-regulated kinase (ERK) and inhibited the activity of activator protein 1 (AP-1), a redox-sensitive transcription factor. In summary, these results suggest that rhapontigenin protects V79-4 cells against oxidative damage by enhancing the cellular antioxidant activity and modulating cellular signal pathways.</P>

Segmentation of Handwritten Character

Min, Gao,Lin, Chen Xi,Jing, Zhang,Wei, Luo 대한전자공학회 1992 HICEC:Harbin International Conference on Electroni Vol.1 No.1

Recognition of handwritten character is an active field which has not been solved yet. In the paper, we discuss the segmentation of handwritten character. An algorithm of connected character segmentation based on the orientation of main axis of reference character and an algorithm of broken character segmentation based on the distance between the centres of two parts are put forward. These algorithm are used in an automatic table input system and has been got a good result.

XPG is Predictive Gene of Clinical Outcome in Advanced Non-small-cell Lung Cancer with Platinum Drug Therapy

Zhang, Tian,Sun, Jing,Lv, Min,Zhang, Lin,Wang, Xia,Ren, Ji-Chen,Wang, Bin Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.2

Polymorphisms in XPG are considered to contribute to the clinical outcome of patients receiving platinum drug chemotherapy. We aimed to investigate the role of five potential SNPs of XPG gene on the response to platinum-based chemotherapy in advanced Chinese NSCLC patients. A total of 451 patients with newly diagnosed and histopathologically confirmed primary NSCLC were consecutively collected. XPG rs2296147, rs4150261, rs17655, rs1047768 and rs2094258 were genotyped by the Taqman real-time polymerase chain reaction (PCR). In our study, we found patients carrying rs1057768 TT genotype had a significantly lower treatment response when compared with the CC genotype (OR=0.38, 95% CI=0.18-0.78). Patients carrying rs1047768 TT genotype showed a significantly short median PFS (11.2 months) and OS (13.6 months) than CC genotype, and the hazard ratios (HR) for PFS and OS were 2.06 (1.01-4.50) and 2.29 (1.21-2.49), respectively. Moreover, we found a significant decreased risk of death from NSCLC among patients carrying the rs2296147 TT genotype when compared with the CC genotype, the HR (95% CI) for OS being 0.50 (0.27-0.95). In conclusion, our study found that polymorphisms in rs1047768 C/T and rs2296147 C/T are associated with response to platinum-based chemotherapy in advanced NSCLC, and XPG polymorphisms could be predictive of prognosis.

Catalytic topological insulator Bi₂Se₃ nanoparticles for <i>in</i> <i>vivo</i> protection against ionizing radiation

Zhang, Xiao-Dong,Jing, Yaqi,Song, Shasha,Yang, Jiang,Wang, Jun-Ying,Xue, Xuhui,Min, Yuho,Park, Gyeongbae,Shen, Xiu,Sun, Yuan-Ming,Jeong, Unyong Elsevier 2017 Nanomedicine Vol.13 No.5

<P><B>Abstract</B></P> <P>Bi<SUB>2</SUB>Se<SUB>3</SUB> nanoparticles (NPs) have attracted wide interests in biological and medical applications. Layer-like Bi<SUB>2</SUB>Se<SUB>3</SUB> with high active surface area is promising for free radical scavenging. Here, we extended the medical applications of Bi<SUB>2</SUB>Se<SUB>3</SUB> NPs further to <I>in vivo</I> protection against ionizing radiation based on their superior antioxidant activities and electrocatalytic properties. It was found that Bi<SUB>2</SUB>Se<SUB>3</SUB> NPs can significantly increase the surviving fraction of mice after exposure of high-energy radiation of gamma ray. Additionally, the Bi<SUB>2</SUB>Se<SUB>3</SUB> NPs can help to recover radiation-lowered red blood cell counts, white blood cell counts and platelet levels. Further investigations revealed that Bi<SUB>2</SUB>Se<SUB>3</SUB> NPs behaved as functional free radical scavengers and significantly decreased the level of methylenedioxyamphetamine. <I>In vivo</I> toxicity studies showed that Bi<SUB>2</SUB>Se<SUB>3</SUB> NPs did not cause significant side effects in panels of blood chemistry, clinical biochemistry and pathology.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Bi<SUB>2</SUB>Se<SUB>3</SUB> NPs can significantly increase the surviving fraction of mice up to 70% after Gamma radiation. </LI> <LI> Bi<SUB>2</SUB>Se<SUB>3</SUB> NPs can help to recover radiation-lowered red blood cell counts, white blood cell counts and platelet levels. </LI> <LI> Bi<SUB>2</SUB>Se<SUB>3</SUB> NPs behaved as functional free radical scavengers and significantly decreased the level of methylenedioxyamphetamine. </LI> </UL> </P> <P><B>Graphical Abstract</B></P> <P>Layer-like Bi<SUB>2</SUB>Se<SUB>3</SUB> with high active surface area can protect mice against ionizing radiation based on their superior antioxidant activities and electrocatalytic properties. Bi<SUB>2</SUB>Se<SUB>3</SUB> NPs can significantly increase the surviving fraction of mice up to 70% after exposure of high-energy radiation of gamma ray. Bi<SUB>2</SUB>Se<SUB>3</SUB> NPs behaved as functional free radical scavengers and significantly decreased the level of methylenedioxyamphetamine. <I>In vivo</I> toxicity studies showed that Bi<SUB>2</SUB>Se<SUB>3</SUB> NPs did not cause significant side effects in panels of blood chemistry, clinical biochemistry and pathology.</P> <P>[DISPLAY OMISSION]</P>

Attenuation of Aortic Injury by Ursolic Acid through RAGE-Nox-NFκB Pathway in Streptozocin-induced Diabetic Rats

Min Xiang,Jianmei Wang,Yaqin Zhang,Jing Ling,Xiaoyue Xu 대한약학회 2012 Archives of Pharmacal Research Vol.35 No.5

Vascular complications are the leading causes of morbidity and mortality in diabetes mellitus (DM). The RAGE (receptor for advanced glycation end products)-NADPH oxidase-NF-κB signal transduction pathway plays an important role in the development of oxidative stressrelated vascular complications in DM. Ursolic acid (UA), a pentacyclic triterpenoid derived from plants, has been reported to have multiple pharmacological effects, including a potent antioxidant activity. This study aimed to investigate both the effect of UA on aortic injury in streptozotocin (STZ)-induced diabetic rats and the drug’s mechanism of action. STZ-induced diabetic animals were randomized in one of the following 4 groups: no treatment (diabetic model group), aminoguanidine (AG, 100 mg/kg), high-dose UA (50 mg/kg), and low-dose UA (25 mg/kg). A non-diabetic control group was followed concurrently. After 8 weeks, the diabetic model rats exhibited: severe aortic arch injury, histologically elevated serum glucose, fructosamine, and glycosylated hemoglobin; and accumulation of advanced glycation end products (AGEs) in the arota. In addition, the levels of RAGE protein, transcription factor NF-κB p65, and the p22phox subunit of NADPH oxidase were increased, as were the serum levels of malondialdehyde and tumor necrosis factor-alpha (TNF-α; p < 0.01 vs control), suggesting that the mechanisms of oxidative stress contributed to vascular injury in the diabetic model group. In contrast, rats treated with UA (50 mg/kg) had a markedly less vascular injury and significantly improved biochemical parameters. Oxidative balance was also normalized in the UAtreated rats, and a marked reduction in the levels of RAGE and p22phox paralleled the reduced activation of NF-κB p65 and TNF-α (p < 0.01 and p < 0.05, respectively, vs diabetic model). These findings suggest that UA may suppress oxidative stress, thus blunting activation of the RAGE-NADPH oxidase-NF-κB signal transduction pathway, to ameliorate vascular injury in the STZ-induced DM rats.

The New Species of the Genus Clubiona from China(Araneae: Clubionidae)

Zhang Yong Jing,Yin Chang Min 외1명 한국거미연구소 1996 한국거미 Vol.12 No.1