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Detection of Puumala and Hantaan Virus among Bats in Korea by Nested RT - PCR
Yun Tai Lee,Bo Kyoung Yun,Jeong Joong Yoon 대한바이러스학회 1998 Journal of Bacteriology and Virology Vol.28 No.2
Hantavirus is a genus of the Bunyaviridae family consisting following serotype groups: Hantaan, Seoul, Puumala, Prospect Hill, Thailand, Belgrade, Thotta palayam, Sin Nombre. Most of Hantavirus group have been associated with many clinically similar disease known collectively as hemorrhagic fever with renal syndrome (HFRS). Hantaan virus is the prototype of the genus hantavirus, originally isolated from Apodemus agrarius. Bat was found as a natural host for Hantaan virus in Lee's lab for the first time. Then, Hantaan-like virus was isolated Hantaan-like virus from bat. To identify hantaviruses that are present in Korea among bats, bats were collected from Jeong-Sun, Won-Joo, Chung-Ju and Hwa-Cheon area, RNA was isolated from lung and serum. RT-PCR was performed with a universal primer from M segment. Nested RT- PCR was carried out to differentiate Hantaan, Seoul and Puumala virus using serotype specific primers. As we expected, Hantaan viruses were detected in bats and Seoul virus was not detected. Interestingly, Puumala viruses were also detected in bats from Won-Ju, but not in other areas. Puumala virus is originally isolated from Clethrinomys glareolus, and cause light HFRS. Recently, Paradoxomis webbiana, a wild bird turn out to be a reservoir for Puumala virus in Korea. These data indicate that bat is a new natural reservoir of Puumala virus.
Detection of Puumala and Hantaan Viruses among Bats in Korea by Nested RT-PCR
Lee, Yun-Tai,Yun, Bo-Kyoung,Yoon, Jeong-Joong The Korean Society of Virology 1998 Journal of Bacteriology and Virology Vol.28 No.2
Hantavirus is a genus of the Bunyaviridae family consisting following serotype groups: Hantaan, Seoul, Puumala, Prospect Hill, Thailand, Belgrade, Thotta palayam, Sin Nombre. Most of Hantavirus group have been associated with many clinically similar disease known collectively as hemorrhagic fever with renal syndrome (HFRS). Hantaan virus is the prototype of the genus hantavirus, originally isolated from Apodemus agrarius. Bat was found as a natural host for Hantaan virus in Lee's lab for the first time. Then, Hantaan-like virus was isolated Hantaan-like virus from bat. To identify hantaviruses that are present in Korea among bats, bats were collected from Jeong-Sun, Won-Joo, Chung-Ju and Hwa-Cheon area, RNA was isolated from lung and serum. RT-PCR was performed with a universal primer from M segment. Nested RT-PCR was carried out to differentiate Hantaan, Seoul and Puumala virus using serotype specific primers. As we expected, Hantaan viruses were detected in bats and Seoul virus was not detected. Interestingly, Puumala viruses were also detected in bats from Won-Ju, but not in other areas. Puumala virus is originally isolated from Clethrinomys glareolus, and cause light HFRS. Recently, Paradoxomis webbiana, a wild bird turn out to be a reservoir for Puumala virus in Korea. These data indicate that bat is a new natural reservoir of Puumala virus.
강력범죄 피해자의 외상 후 스트레스 증상 및예측요인에 대한 탐색적 연구
김태경(Kim, TaeKyoung),윤성우(Yun, SungWoo),이영은(Lee, YoungEun),이새롬(Lee, Saerom) 한국피해자학회 2018 被害者學硏究 Vol.26 No.1
The aim of this study was to investigate the posttraumatic stress symptoms of violent crime and predictors to the symptoms. For this, we analyzed data of 473 victims registered to the integrated supporting center for violent crime victims who agreed to participate in the study. The results showed that more than 90% of victims experience significant PTSD symptoms, 30% of them were in extreme mental confusion that required medication or hospitalization. There was a significant difference in the severity of sequela according to gender, time since visiting to the center, pre-event adaptation level, and event type. Although the victim experienced more avoidance and hypervigilance symptoms than the family, the psychological distress experienced by the family was not less than that of the victim. Psychological sequela of the events were found to be more severe with more experiences of childhood trauma, lack of ego resilience, low level of perceived social support, strong dissociation tendency, and avoiding coping styles. A result of the stepwise regression analysis, avoidant coping style explained 10.6% of the total variance. Limitations and implications of this study were discussed.
억제대 적용지침 개발을 통한 불필요한 억제대 적용율 감소활동
곽경선,김성은,배은경,이은숙,이은혜,최윤정,이윤경 한국의료QA학회 2009 한국의료질향상학회지 Vol.15 No.2
문제: 억제대 적용에 대한구체적 적용지침 없이 의례적으로 억제대를사용함으로 인해 환자들의 신체적 또는 정신적 안전을 위협할 수 있다. 목적: 완전 진정상태 이거나 근력이 약하여 발관능력이 없는 환자에 대한 예방적 적용, 바빠서 환자를볼수 없 는경우, 간호사본인의 판단이 없이 타인의 요구에 따르는등의 불필요한 억제대 적용이 증가하는 원인을 파악하여 중환자실의 특수성에 맞는 억제대 적용및 제거 지침을 마련하고자 한다. 의료기관: 인천광역시에 소재한 의과대학 소속병원의 집중치료실 개선효과: 억제대 적용에 대한중환자실 간호사에게 미치는 영향을 보면 태도 정도 p=0.09(p<0.1), 올바른 수행 능력은 p= 0.005 (p <0.0히로통계적으로유의한것으로 나타났다. 지식 정도 p=0.172(p<0.05) 통계적으로 유의 하지는 않지만 모든항목이 개선 활동전 에 비해 2,3차 개선 활동후에 향상한 것으로 나타났다. 개선활동전에 8.1%였던 간호 기록, 0.7%였던 간호중재, 0%였던 의사처방율이 3차 개선 활동 후 3가지 모두 100% 달성되었다. 이는 억제대 적용율 갑소 및 부적절한 억제대 적용율은 유의하게 감소하였다. 교훈: 중환자실 간호사의 억제대 적용에 대한태도, 지식, 올바른 간호 수행 능력은 지속적으로 재평가 되어지고, 교육내용은좀더 나은 간호제공을 위하여 개발 되어져야 할 것이다. 억제대 적용 기준과 제거 기준을 Check List 하여 객관성의 유지를 위한 개선 활동은계속적으로 Feedback 되어야 할 것이다.
절수에 의한 mongolian gerbil의 체중 변화에 관한 연구
이경열,송치원,김무강,조성환,류시윤,김상근,박배근 충남대학교 수의과대학 동물의과학연구소 1996 動物醫科學硏究誌 Vol.4 No.-
Recently, the mongolian gerbils have been used in neurology and the study of water metabolism because of their easy onset of epileptic seizure and typical resistance to water deprivation. The sebaceous glands which are under the control of the gonadal hrmones are differently developed from the other laboratory animals. However the physiological data on the changes of body weight following water deprivation in mongolian gerbils is not available until now. In order to investigate the effect of the water deprivation on the body weight, the animals were deprived of water for 15 days. The body weights were measured daily by the torsion balance. The mean, the standard deviation, the standard error and the coefficient of variance of the body weights were calculated. The correlation was examined. The potential regression equation was y = 61.5458 - 2.0533 * x + 0.0487 * x^2. The regression coefficient was 0.993. The results obtained were as follows : 1. The body weights of the mongolian gerbils were continuously decrased from the 1st day to the 15th day after the water deprivation. 2. The mean body weight on the water deprivation was 63.0±7.00g and that at the 15th day was 41.4 ± 5.42g. 3. The coefficients of the variance showed the highest (13.09) at the 15th day and the lowest (10.10) at the 10th day. the coefficients of the variance kept decreasing until the 10th day and thereafter gradually increased until the 15th day. 4. The changing rate of the body weight kept continuously decreasing from the 1st day to the 15th day showing the daily loss of 1.0 - 3.3%. 5.The deviations (M - QBW) between the measured values (m) and the theorical values (QBW) using the regression equation showed the highest (1.494) at the 1st day and the lowest (0.053) at the 13th day. 6. The sum of the devitions was 0.179 and that of the deviation square was 1.15. The sum of the deviation rates between the mesaured body weights and the theorical ones was 0.484. 7. The daily body weight loss showed the highest at the 1st day by 2.053g and the lowest at the 15th day by 0.591g. 8. The specific body weight changing rates which were the rate of the body weight changing velocity to the changed body weight were decreased from 0.033% at the 1st day to 0.014% at the 15th day.
완전통합교육의 실현 근거와 과제에 대한 특수교사와 일반교사의 인식조사
이윤수,김자경 조선대학교 학생지도연구소 2003 生活指導硏究 Vol.22 No.1
The Full Inclusive Education, reflecting the world-wide trends of special education, can be defined as "the education of all disabled students with non-disabled ones of same age, throughout the support system on the basis of individual's needs at nearby schools, regardless of the types and levels of disabilities." Thus, the education is the very one that disabled students should be taught along with all people with an equal status, say, no discriminations. To secure the full-inclusive education of effectiveness and substantiality, understanding and interests of teachers related to disabled students are needed the most, acting as the major factor, along with administrative & financial supports and favorable societal milieu. Against the backdrops, this study is intended to divulge the differences of conscious levels between two groups, special education teachers and general education teachers, regarding actualization basis and task for full-inclusive education. The subject of this study were total 315 teachers(129 special education teachers and 186 general education teachers) in Gwangju and Jellanamdo province. The results are as following: First, the perception level in actualization basis for full-inclusive education between two groups was high. Second, special teachers, in general, had more positive perception than general teachers, concerning full-inclusive education. This outcome agrees with the previous study result that full-inclusive education first started in special education area, thereby revealing its trust differences between the two groups. However, the perception level of general teachers in elementary schools, with regard to actualization basis for full-inclusive education, was significantly low. This results are in stark contrast with the ones of earlier studies. Thus, it is surmised that general elementary school teachers are fully informed of the difficulties in teaching disabled students in inclusive milieu but general teachers in middle and high schools just perceive its importance as being legitimate As a result, further studies on that issue are required. Third, with regard to perception on actualization task for full-inclusive education, special teachers showed higher response than general teachers. Also, special teachers marked higher level of perception on educational courses & methods, and consciousness than general teachers, but no significant difference on educational environment and teachers' speciality fields between the two groups. But, special teachers showed a low perception on the question that more diversified system, is needed to provide general teachers with license for special teachers, indication that the introduction of the institution can undermine the establishments of special teachers. To sum up, not merely special teachers but also general teachers all agree with legitimacy of full-inclusive education in many respects. Nevertheless, it also shows that there are many tasks to deal with in various parts on the way to full-inclusive education.
신규개발된 제초제 metamifop 현탁농축액제에 대한 급성독성평가
이동연,이상훈,백경진,김태원,정두순,임종환,김명석,박병권,윤효인 충남대학교 수의과대학 동물의과학연구소 2004 動物醫科學硏究誌 Vol.12 No.-
Metamifop, (R)-2-[4-[(6-chloro-2-benzoxazolyloxyphenoxy]-N-(2-fluorophenyl)-N-methyl propanamide, was newly developed by Dongbu Hannong Chemicals in Korea. In the present study we conducted acute oral toxicity and acute dermal toxicity test. The values of LD_(50) of metamifop was found to be over 5,000 mg/kg for ICR mice. On the other hand, metamifop showed no acute dermal toxicity to SD rats.