프리미돈의 한국인에서의 생체이용율시험

권광일,윤민혁,윤희열,박희찬,권준택,심희옥,김동출 충남대학교 약학대학 의약품개발연구소 2004 藥學論文集 Vol.19 No.-

A reversed phase HPLC method was developed and validated for the determination of primidone in human plasma. Propylthiouracil was used as an internal standard. Calibration curves were linear in the concentration range of 10-120 ng/㎖. The coefficient of variation of the intra- and inter-day precision were below 15%. The coefficient of variation of the accuracy were below 15% in the concentration range investigated. A bioavailability study was performed using the validated HPLC method. Twenty four healthy human male volunteers were orally administered 20 mg of primidone. The pharmacokinetic parameters were calculated using WinNonlin. The mean values of AUC_(LAST) was 85.0±17.7 ㎍ hr/㎖, C_(max) was 4.22±1.04 ㎍/㎖, T_(max) was 1.85±1.13 hr, t_(1/2) was 17.7±2.4 hr. The pharmacokinetic parameters and the HPLC method can be used for the desigm of bioequivalence study of primidone.

C-means 알고리즘을 이용한 마이크로 엔드밀의 상태 감시

권동희,정연식,강익수,김전하,김정석 한국공작기계학회 2005 한국공작기계학회 춘계학술대회논문집 Vol.2005 No.-

Recently, the advanced industries using micro parts are rapidly growing. Micro end milling is one of the prominent technology that has wide spectrum of application field ranging from macro to micro parts. Also, the method of micro-grooving using micro end milling is used widely owing to many merit, but has problems of precision and quality of products due to tool wear and tool fracture. This study deals with condition monitoring using acoustic emission(AE) signal in the micro-grooving. First, the feature extraction of AE signal directly related to machining process is executed. Then, the distinctive micro end mill state according to the each tool condition is classified by using the fuzzy C-means algorithm, which is one of the methods to recognize data patterns. These result is effective monitoring method of micro end mill state by the AE sensing techniques which can be expected to be applicable to micro machining processes in the future.

數種의 腐熟劑 添加가 볏짚의 堆肥化에 미치는 影響

尹奉基,金炳浩,金喜權,朴仁珍 목포대학교 자연자원개발연구소 2000 자연자원연구 Vol.3 No.-

This study was conducted to find out effect of microorganisms added for decomposition of organic matter when manufacturing compost. The microorganisms used were EM, LACTO, SEMIP, BAIMUD circulated in market and 068065 developed in Institute of Agricultural Sciences. Effect of microorganisms inoulation on rice straw was summarized as follows: The number of bacteria, actinomycetes and fungi was 28.7∼76.7(×106), 0∼72.0(×106) and 0∼198.7(×105)cfu/g respectively. The number of bacteria, actinomycetes and fungi between microorganisms was no different. The maximum temperature during composting periods was reached 69∼76℃- at 3 50 5 days after heaping and kept over 65℃ for 7 to 10 days. The number of bacteria, actinomycetes and fungi in compost manufactured with rice straw was 42.8∼206.0(×106), 11.3∼54.7(×106), and 9.8∼42.5(×105)cfu/g respectively. The tendency was similar to control. Degree of decomposition of plot added microorganisms except 068065 and BAIMUD was similar to control at 50 days after heaping. The contents of inorganic elements were 1.26∼1.35% T-N, 1.14∼1.38% P2O5, 0.72∼0.97% K2O, 1.01∼1.74 CaO and 0.63∼1.44% MgO. Contents of T-N, P2O5 , CaO, MgO in compost were increased, but content of K2O was decreased, It was no different between treatments.

Bacillus cereus에 있어서 Phospholipase C활량에 관한 몇가지 계면활성제의 영향

권오용,윤희주,한수자 충남대학교 자연과학연구소 1987 忠南科學硏究誌 Vol.14 No.2

This study demonstrates the effects of four detergents and three on the activity change of phospholipase C which is prepared from the cultural broth of Bacillus cereus. This assay of phospholipase C activity was carried out with phosphatidylcholine micelles which were mixed with the detergents and sterols, with increase a molar ratio of these compounds to phosphatidylcholine. Phospholipase C was highly activated at 0.5 molar ratio of Tween 80-mixed micelles. In the case of Triton X-100, it was activated at higher molar ratio indicated 1.0. Sodium dodecyl sulfate was more effective than that of the other detergents at lower molar ratio. However sodium deoxycholate-mixed micelles increased the activity more 7 times than that of the control group. This maximum activation was occurred at 2.5 molar ratio and the turbidity was gradually decreased. Cholesterol and ergosterol increased the activity until molar ratio reached up to 0.9. But the activity was higher at 0.3 molar ratio for stigmasterol. Consequently, it was indicated that phospholipase C was activated with the supplement of detergents until the mixed micelles made a number of small and spherical shape.

紫雲英裁培沓에서 窒素와 石灰의 施用이 紫雲英의 腐熟과 벼 生育에 미치는 影響

윤봉기,김희권,최형국,박인진 한국국제농업개발학회 2004 韓國國際農業開發學會誌 Vol.16 No.2

녹비작물로서 자운영을 재배한 논에 수포를 재배할 때 시용한 자운영의 부숙촉진방법을 구명하기 위하여 자운영의 개화 성기와 결실기에 자각 예취하여 시용하고 질소 0, 30, 60, 질소 30+석회 1000, 석회 1000㎏/㏊의 5가지 부숙촉진 처리로 2000년부터 2년동안 포장시험한 결과는 다음과 같다. 1. 자운영의 개화성기 생육은 초장 44.5㎝, 생초수량 23.88Mg/㏊였으며 결실기에는 초장43.6㎝, 생초수량 19.53Mg ㏊^(-1)이였다. 무기성분 함량은 자운영 개화성기에 T-N 2.6%. T-C 78.6%, P₂O_(5) 0.57%, K₂O 2.73%였으며 결실기에는 T-N 2.3%, T-C 82.8%, P₂O_(5) 0.53%. K₂O 2.15%였다. 2. 개화성기에 시용한 자운영의 부숙정도는 경운후 10일에 82.5~86.8%로 급증하여 이후 완 만한 경향을 보였으며 결실기의 부숙정도는 경운후 10일에 62.2~67.1%로 개화성기보다 낮았다. 부숙처리에 따라서는 개화성기에 예취하여 시용하고 부숙처리한 시험구중 석회 1,000㎏/㏊처리에서 최고의 부숙정도를 보였으며 결실기 처리구에서는 질소 30+석회 1,000㎏/㏊ 처리구에서 높은 부숙도를 의였다. 3. 벼의 생육은 출수기의 초장은 102.0~l12.4㎝, 수확기의 간장은 76.6~84.2㎝, 수장 17.9~20.0㎝로 개화성기의 부숙 처리에서 초장. 간장, 수장이 큰 편이었다. 벼의 수량은 4.89~5.33Mg/㏊로 자운영 개화성기의 부숙촉진 무처리(5.01 Mg/㏊)에 비하여 석회 1,000㎏ ㏊처리에서 4% 증수되었고 결실기의 부숙촉진 처리에서는 무처리 (5.25Mg/㏊)에 비하여 질소 30+석회 1,000㎏ ㏊^(-1)처리에서 1%증수되었으나 기타 처리에서는 1~2%감수되었다. 4. 시험후 토양의 화학성은 pH 5.4~6.0. OM 12.5~13.5g/㎏, CEC 12.4~14.5c㏖/㎏, Ava. P₂O_(5) 38~56㎎/㎏으로 자운영 부숙촉진 처리에저 Ava. P₂O_(5), Ava. SiO₂, CEC, 치환성 K의 함량이 많은 편이었다. The experiment was conducted to find out quick decomposition method of applied milk vetch at rice paddy soil. Decomposition methods were 5 treatments of N fertilizer 0, 30, 60, N 30+Lime 1000 and Lime 1000 ㎏/㏊ at paddy field applied flowering and bearing stage of milk vetch. It was summerized as follows; At flowering stage of milk vetch, the content of T-N, T-C, P₂O_(5), K₂O in milk vetch plant was 2.6, 78.6, 0.57, 2.73% respectively. plant height was 44.5 ㎝. green yield was 23.88 Mg/㏊. At bearing stage of milk vetch, the content of T-N, T-C, P₂O_(5), K₂O in milk vetch was 2.3, 82.8, 0.53, 2.15% respectively. Plant height was 43.6 ㎝. Green yield was 19.53 Mg/㏊. Decomposition rate of milk vetch at 10 days after application at the flowering stage was 82.5~86.8% and that of bearing stage was 62.2~67.1%. When milk vetch was cut at flowering stage, decomposition rate of milk vetch treated lime 1000 ㎏/㏊ was more than any other treatment. When milk vetch was cut at bearing stage, decomposition rate of milk vetch treated N 30+lime 1000 ㎏/㏊ was highest. Plant height of rice was 102.0~112.4 ㎝ at heading stage and stem height was 76.6~84.2 ㎝, panicle length 17.9~20.0 ㎝ at harvesting stage. Plant height, stem height, panicle length of decomposition treatment plot was trend to longer when milk vetch was cut at flowering stage. When milk vetch was cut at flowering stage, rice yield of lime 1000 ㎏/ ㏊ applied plot increased 4% more than that of control (5.01 Mg/ha). When milk vetch was cut at bearing stage, N30+lime 1000 ㎏/㏊ applied plot was increased 1% than that of control (5.25 Mg/ha). But the rice yield other plot decreased 1~2% than that of control. Chemical properties of soil after experiment were pH 5.4~6.0, OM 12.5~13.5 g/㎏, CEC 12.4~14.5 c㏖/㎏. The content of Av. P₂O_(5), Av. SiO₂, CEC, Exch. K in soil applied milk vetch was more than that of control.

남부 중간지 자운영재배답에서 벼재배시 논 유형에 따른 질소시비량 구명

윤봉기,김병호,김희권,이인,최형국,박인진 한국국제농업개발학회 2003 韓國國際農業開發學會誌 Vol.15 No.1

중간지의 보통답과 사질답에서 녹비작물인 자운영을 재배한 논에 벼를 재배할 때 답 유형에 따른 질소비료 시용량을 구명하고자 전남 구례의 보통답과 사질답에서 관행, 자운영+질소 70%, 자운영+질소 50%, 자운영+질소 30%, 자운영+무질소의 5처리로 2000년부터 2년동안 시험한 결과는 다음과 같다. 1. 자운영의 개화성기 생육은 초장 44.1∼44.2㎝, 생초중 20.1∼22.5Mg ㏊^-1이였으며 무기성분 함량은 T-N 2.46∼2.68%, P_2O_5 0.56∼0.60%, K_2O 2.05∼2.18%였다. 사질답에 비하여 보통답에서 자운영의 생초중이 많았다. 2. 토양중 NH_4-N함량의 경시적 변화는 이앙 후 7일에 21.5∼48.3㎎ ㎏^-1으로 가장 높았으며 이앙후 70일에 7.0∼9.7㎎ ㎏^-1으로 점차 감소하였는데 처리에 따라서는 자운영+질소 70%, 자운영+질소 50%시용구에서 높았다. 3. 벼 수확기의 간장 76∼85㎝, 수장 17.9∼19.2㎝로 사질답이 보통답에 비하여 간장, 수장이 컸다. 4. 시험후 토양의 화학성은 pH 5.4∼5.8, OM 21.5∼24.4g ㎏^-1, CEC 9.2∼10.9cmol ㎏^-1, Ava. P_2O_5 141∼360㎎ ㎏^-1으로 자운영처리구에서 OM, CEC함량이 증가하였다. 5. 벼의 수량은 관행(보통답 5.03Mg ㏊^-1, 사질답 5.18Mg ㏊^-1)에 비하여 보통답의 자운영+질소 70%, 50%시용구에서 각각 5, 4%증수되었고, 사질답에서는 자운영+질소 70%, 50%시용구에서 각각 7, 4%증수되었다. 6. 남부 중간지에서 자운영을 재배하여 생초 20Mg ㏊^-1를 녹비로 투입하면 벼를 재배시 질소 시용 적정량은 보통답, 사질답 모두 관행 시비량의 50%이였다. This study was conducted to find out fertilizer recommendation rates for rice at a normal and sandy paddy field after planting milk vetch(MV) in Guryae which lies to southern medium altitude region from 100m to 250m above sea level from 2000 to 2001. The amount of N fertilizer were 110(normal paddy) and 130(sandy paddy) ㎏ ㏊^-1 at control and 0, 30, 50, 70% of the control. At flowering stage of milk vetch, the content of T-N, P_2O_5, K_2O in plant were 2.46∼2.68%, 0.56∼0.60%, 2.05∼2.18%, respectively. Plant height were 44.1∼44.2㎝. Fresh weight were 20.1∼22.5Mg ㏊^-1. Fresh weight of milk vetch cultivated in normal paddy field was more than that in sandy paddy field. The content of NH_4-N in soil was 21.5∼48.3㎎ ㎏^-1 at 7 days after planting and 7.0∼9.7㎎ ㎏^-1 at 70 days after planting. Content of NH_4-N in soil was increased at the treatments of milk vetch + N 70, 50% of control. Culm length was 76∼85㎝, panicle length was 17.9∼19.1㎝ at harvesting stage. Culm length and panicle length at sandy paddy field were longer than those of normal paddy field. Chemical properties of soil after experiment were pH 5.4∼5.8, OM 15.6∼21.5g ㎏^-1, CEC 9.2∼10.9cmol ㎏^-1, Ava. P_2O_5 141∼360㎎㎏^-1, respectively. The content of OM, CEC in soil applied milk vetch at normal paddy field was higher than those of the control, and the content of CEC in soil applied milk vetch at sandy paddy field was higher than that of the control. When milk vetch was cut at flowing stage, rice yield of milk vetch + N 70, 50% of control plot were increased 5, 4% more than that of control(5.03Mg ㏊^-1) at normal paddy field. Rice yield vetch+N 70, 50 of control were increased 7, 4% more than that of control(5.18Mg ㏊^-1) at sandy paddy field. Nitrogen application rate of normal and sandy paddy field was 50% of conventional fertilization rate when 20Mg ㏊^-1 milk vetch cultivated paddy field was inputed for rice culture in the southern medium altitude.

동화 관련 활동이 유아의 창의성 증진에 미치는 효과

권창길 ; 이기영 ; 고윤희 ; 최성열 김천대학교 2005 김천대학교 논문집 Vol.26 No.-

This study is to find out about the effect of children's stories and related activities on the creativity of children by conducting a research on 90 5-year-olds attending Kindergraten B in Gumi City and Kindergarten F ing Gimcheon City.

잔류응력 완화 중 나노접촉 형상의 천이거동 모델링을 통한 DLC 박막의 잔류응력 평가

李潤熙,池源宰,鄭增鉉,權東一 대한금속재료학회 2002 대한금속·재료학회지 Vol.40 No.7

Assessment of residual stress is an important point to prevent the failures of thin film devices. Nanoindentation technique was proposed as a stress-measuring method of thin film in a previous research. However, the stress-analyzing model used at the previous research had the problems of a partial deformation-irrelative stress interaction concept and no consideration of the change in the contact area during continuous stress relaxation. Therefore, we modified the model by analyzing a new residual stress-induced normal load based on deformation-dependent shear stress component and by calculating the continuous relaxation procedure as an integration method. The modified model was applied to evaluate the residual stresses in two diamond-like carbon films. The evaluated residual stresses from the newly modified model were more consistent with the results from the conventional curvature method than those from the earlier model.

우리 나라 보건관리대행서비스 평가 연구

하은희,조수헌,김선민,주영수,한상환,하미나,권호장,홍윤철,김창엽 大韓産業醫學會 1998 대한직업환경의학회지 Vol.10 No.1

Group occupational health service programme started in 1990 is one of the measures to cope with limited human and financial resources in occupational health. The programme has expanded rapidly to include 52 institutions, private as well as public, all over the country. In spite of its potential impact on health of employees and practice of occupational health in small and medium sized industries, comprehensive evaluation in terms of quality has not been tried. This study has aims to develop the criteria to assess the quality of newly developed group occupational health service programme, and to investigate the quality of institutions, and finally to develop policies for the quality improvement. 1) Criteria development : By defining occupational health services, in particular for small and medium sized industries, as one of the primary health care, followings are included as core elements of qualitative occupational health programme; accessibility, continuity, comprehensiveness, technical quality, intersectoral collaboration, emphasis on preventive services, community participation, and adequacy. Again each element is divided into five major components of national health system infrastructure developed by the World Health Organization; development of health resources, organized arrangement of resources, delivery of health care, economic support, and management. In turn, each component is categorized into three aspects of quality assessment, structure, process and outcome. Expert panel selected several criteria for each category to evaluate the programme. Criteria were modified according to each group of interviewees, to produce two sets of questionnaire, one for chief operating officer and another for nurses in the institutions, and the chief operating officer and workers in the workplace. 2) Subject : Of all 52 institutions, 25 voluntarily participated in the survey. At individual institution, chief operating officer and practicing nurses were interviewed in depth. After intensive education for interviewees, every interviewees, every interview was performed with standardized guideline and questionnaire. The quality of the 'Group occupational health service programme' was found to be lower than expected. Especially in continuity, comprehensiveness, technical quality, community participation and adequacy, lower quality in structural aspect was commonly identified throughout all the institutions,. Quality in terms of accessibility and continuity highly varied among institutions., To improve quality of the programme, more comprehensive and systematic programme such as accreditation has to be introduced. In addition, human resources, governmental fund and information systems for individual workers are to be developed. As a long range plan, integration of occupational health services into the national health systems and pooling of financial resources and planned allocation should be considered.

나노 압입곡선에 미치는 잔류응력 영향의 미세역학적 분석

李潤熙,權東一 대한금속재료학회 2002 대한금속·재료학회지 Vol.40 No.12

A new model is presented for measuring equi-biaxial stress on thin-film using nanoindentation. The model was focused on the analysis of a depth-shift in the nanoindentation curve by stress effect. A load-controlled relaxation of the shifted indentation depth was chosen to avoid the variation of the contact area occurred in a depth-controlled relaxation. Nanoindentation experiments on (100) W single crystal at artificial equi-biaxial stress states verified that the proposed load-controlled relaxation model is capable of analyzing the residual stress within the standard deviation of ±35.9 MPa.