The CCR4-NOT Complex Is Implicated in the Viability of Aneuploid Yeasts

Tange, Yoshie,Kurabayashi, Atsushi,Goto, Bunshiro,Hoe, Kwang-Lae,Kim, Dong-Uk,Park, Han-Oh,Hayles, Jacqueline,Chikashige, Yuji,Tsutumi, Chihiro,Hiraoka, Yasushi,Yamao, Fumiaki,Nurse, Paul,Niwa, Osami Public Library of Science 2012 PLoS genetics Vol.8 No.6

<P>To identify the genes required to sustain aneuploid viability, we screened a deletion library of non-essential genes in the fission yeast <I>Schizosaccharomyces pombe</I>, in which most types of aneuploidy are eventually lethal to the cell. Aneuploids remain viable for a period of time and can form colonies by reducing the extent of the aneuploidy. We hypothesized that a reduction in colony formation efficiency could be used to screen for gene deletions that compromise aneuploid viability. Deletion mutants were used to measure the effects on the viability of spores derived from triploid meiosis and from a chromosome instability mutant. We found that the CCR4-NOT complex, an evolutionarily conserved general regulator of mRNA turnover, and other related factors, including poly(A)-specific nuclease for mRNA decay, are involved in aneuploid viability. Defective mutations in CCR4-NOT complex components in the distantly related yeast <I>Saccharomyces cerevisiae</I> also affected the viability of spores produced from triploid cells, suggesting that this complex has a conserved role in aneuploids. In addition, our findings suggest that the genes required for homologous recombination repair are important for aneuploid viability.</P><P><B>Author Summary</B></P> <P>Aneuploidy is a major cause of abortive development and is implicated in tumorigenesis in humans. Recent studies revealed that the increased need for protein degradation might account for the detrimental effects of aneuploidy on a cell. Here, we investigated the genetic systems responsible for aneuploid viability. Using a collection of gene deletions in fission yeast, we isolated mutants that affect aneuploid viability. We found that an evolutionarily conserved transcription regulator, the CCR4-NOT complex, and its related factors are required for aneuploid viability, suggesting that regulation of mRNA turnover is required to tolerate aneuploidy. In addition, homologous recombination repair is important for aneuploid viability.</P>

Dynamic performance of 6.5 kV SiC MOSFET body diodes and anti‑parallel Schottky barrier diodes

Yujie Du,Xinling Tang,Xiaoguang Wei,Shuai Sun,Fei Yang,Zhibin Zhao 전력전자학회 2023 JOURNAL OF POWER ELECTRONICS Vol.23 No.6

For a silicon carbide (SiC) metal–oxide–semiconductor fi eld-eff ect transistor (MOSFET), both the body diode of the MOSFET and an anti-parallel diode can function as a freewheeling diode that carries reverse current. Selecting a suitable freewheeling method is particularly important to fully exploit the performance of high-voltage SiC MOSFET devices. In this study, based on the 6.5 kV SiC MOSFET and the Schottky barrier diode (SBD) developed by our research group, the dynamic characteristics of the MOSFET body diode, the SBD, and the parallel connection of the two are investigated. In addition, the influence of capacitive current on the dynamic characteristics is analyzed by establishing an equivalent circuit model. Test results show that when compared with the body diode, the SBD has an extremely low reverse recovery current, a lower capacitive current, and better reverse recovery temperature stability. Thus, the SBD is regarded as the most suitable freewheeling diode for the 6.5 kV SiC MOSFET. Finally, a package solution for the 6.5 kV SiC MOSFET and SBD is proposed.

Piperine as a Potential Nutraceutical Agentfor Managing Diabetes and Its Complications: A Literature Review

Yujie Wang,Shan Xu,Lidan Tang,Jinhong Gong,Dan Su,Hao Yang 한국식품영양과학회 2023 Journal of medicinal food Vol.26 No.10

The global prevalence of diabetes and its related complications has increased drastically and is currently aworldwide health challenge. There is still an urgent need for safe and effective natural products and supplements as alternativeand/or adjunctive therapeutic interventions. Nowadays, people pay more and more attention to the nutritional and medicinalvalue of food ingredients. As one of the most widely employed spices in cooking, pepper also has novel medicinal valuesattributed to its main component, piperine (Pip). Pip is an amide alkaloid with pleiotropic properties such as antiinflammatory,antioxidant, anti-cancer, and other related activities. Recently, Pip has received increasing scientific attentiondue to its antidiabetic and related complication properties. However, the values of existing studies are limited due to beingscattered and unsystematic. The present study reviewed the therapeutic potential and possible mechanisms of Pip in diabetesand related complications, with the aim of providing promising candidates for the development of novel and effectivealternative and/or adjunctive nutraceutical agents for the management of diabetes.

Tensile Behaviors of Laser-Discrete-Quenched Substrate/Nickel Coating of Electroplated Grinding Wheel

Cong Mao,Yujie Zhong,Yongle Hu,Weidong Tang,Z. M. Bi,Zhaohong Lei,Changchun Jiang,Aimin Tang 한국정밀공학회 2022 International Journal of Precision Engineering and Vol.9 No.5

Electroplated grinding wheel has significant advantages, such as longer service life, higher removal rate, reduction of preparation work, reapplication of the wheel bub. Then it is an efficient, green and eco-friendly abrasive tool. In order to reveal the mechanism that the service life of the electroplated grinding wheel is increased by pre-quenched substrate, the tensile behavior and interfacial stresses of the laser-discrete-quenched substrate/nickel coating were studied through tensile experiment and simulation. The results show that random initial cracks appear in the nickel coating over the unquenched zone before the tensile experiment. There is an inter-diffusion region at the interface of steel substrate and nickel coating; in comparison with the unquenched zone, the size of the inter-diffusion region in the laser-quenched zone was decreased significantly (e.g., 1.2 μm in comparison with 2.6 μm). In tensile testing, the coating over the pre-quenched specimen remains bonded with the substrate firmly even the fracture occurs. In contrast, the damages in the forms of blistering, stripping and exfoliating occurred in the coating over the unquenched specimen. When a fracture occurs, there is a significant difference of the ultimate strains of quenched and unquenched zones; the ultimate strain of quenched zone is 0.0714, while that of the unquenched zone is 0.1667. At the bonding interface, the pre-quenched specimen ensured the overall shear stress and the maximum normal stress, which are lower than those of unquenched specimen. The initial cracks in pre-quenched specimen propagate during stretching, the effect of the absorbed strain energy reduces strain mismatch between the substrate and coating at the interface, and this improves the bonding strength at the interface of the laser-discrete-quenched specimen significantly.

Highly efficient and selective extraction of minor bioactive natural products using pure ionic liquids: Application to prenylated flavonoids in licorice

Shuai Ji,Yujie Wang,Shikai Gao,Xian Shao,Wei Cui,Yan Du,Mengzhe Guo,Daoquan Tang 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.80 No.-

Lots of minor constituents in herbal medicines have shown great potential to devolve into leadcompounds, and their extraction is attracting more and more attention in pharmaceutical industry. Thisstudy provided an example that showed efficient and selective extraction of minor bioactive naturalproducts (prenylatedflavonoids with anti-cancer activities) from an herbal medicine (licorice) using pureionic liquids as green solvents. After investigation of a variety of hydrophobic ionic liquids withultrasonic-assisted extraction, we found that [C8MIM]BF4 (1-octyl-3-methylimidazolium tetrafluor-oborate) was the most effective one for extraction offive representative compounds (isoangustone A,glycycoumarin, licoisoflavanone, licoricidin and glabridin), and solvent to solid ratio, extraction time,extraction temperature and soaking time were then optimized through single-factor experiments andresponse surface methodology. As a result, the established method had significantly higher extractionselectivity than conventional organic solvents, which could be attributed to significant physical change oflicorice microstructures after extraction, as well as strong interactions (especially hydrogen bondinteractions) between [C8MIM]BF4 and prenylatedflavonoids by mechanism study. In addition, 78.92% ofthe extracted prenylatedflavonoids could be readily recovered from [C8MIM]BF4 by using reversed-phasesolid phase extraction, and the recyclability of [C8MIM]BF4 was also confirmed.

A Learning-based Power Control Scheme for Edge-based eHealth IoT Systems

( Haoru Su ),( Xiaoming Yuan ),( Yujie Tang ),( Rui Tian ),( Enchang Sun ),( Hairong Yan ) 한국인터넷정보학회 2021 KSII Transactions on Internet and Information Syst Vol.15 No.12

The Internet of Things (IoT) eHealth systems composed by Wireless Body Area Network (WBAN) has emerged recently. Sensor nodes are placed around or in the human body to collect physiological data. WBAN has many different applications, for instance health monitoring. Since the limitation of the size of the battery, besides speed, reliability, and accuracy; design of WBAN protocols should consider the energy efficiency and time delay. To solve these problems, this paper adopt the end-edge-cloud orchestrated network architecture and propose a transmission based on reinforcement algorithm. The priority of sensing data is classified according to certain application. System utility function is modeled according to the channel factors, the energy utility, and successful transmission conditions. The optimization problem is mapped to Q-learning model. Following this online power control protocol, the energy level of both the senor to coordinator, and coordinator to edge server can be modified according to the current channel condition. The network performance is evaluated by simulation. The results show that the proposed power control protocol has higher system energy efficiency, delivery ratio, and throughput.

Comparative study of O₂ adsorption kinetics on p- and n-Si(001) surfaces at room temperature

Ryo TAGA,Kiwamu NISHIMOTO,Jiayi TANG,Zhou YU,Shuichi OGAWA,Yuji TAKAWA 한국진공학회 2016 한국진공학회 학술발표회초록집 Vol.2016 No.8

Enhanced corrosion resistance by polypyrrole and Ti3C2Tx-acrylic epoxy double-layer coating for 304SS bipolar plates of PEMFC

Haifeng Bian,Guoli Zhang,Qingxi Zhai,Yanyan Du,Yujie Ma,Beibei Yang,Shaochun Tang,Duan Bin,Xiangkang Meng,Hongbin Lu 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.122 No.-

Stainless steel (SS) is a promising material for designing bipolar plates (BPs), but their further applicationis limited by serious corrosion problems in the acidic environment. Ti3C2Tx is expected to be used for SSBPs coatings, while galvanic corrosion will occur after the damage of Ti3C2Tx coating. Herein, a PPY/Ti3C2Tx-AE double-layer coating (DC) is well-designed and prepared on 304SS BP, which is composedof an inner electropolymerized PPY layer and an outer Ti3C2Tx-acrylic epoxy layer. When tested in0.2 M HCl solution, the corrosion potential and corrosion current density of the DC are 38 mV and0.00927 lA cm2 respectively, which are superior to those of the PPY coating and the Ti3C2Tx coating. Moreover, the DC presents the best long-term stability among the three coatings. The excellent corrosionresistance is attributed to the barrier and anodic protection effects as well as the solution of galvanic corrosion. The new coating system provides a new insight into the design of DC coatings of SS BPs.

Research on Magnetic Field of Multistage Counter Roll Magnetorheological Fluid Transmission Device

Xiangfan Wu,Yangyang Guo,Zuzhi Tian,Fangwei Xie,Yujie Tang 한국자기학회 2022 Journal of Magnetics Vol.27 No.2

Aiming to solve the problem of magnetorheological transmission heat dissipation, a novel magnetorheological fluid transmission device is designed, and the torque of the device is analyzed. Based on electromagnetic theory, the magnetic circuit of the device is designed. The finite element method is used to simulate the magnetic field of the measurement device. Results show that the working magnetic induction can reach 0.5 T when the current is 1.6 A, which can meet design requirements. The magnetic induction intensity in the working space increases with the increase of the excitation current and permeability of the magnetic conductive material; decreases with the increase of the size of the working space; and increases with the increase of the size of the magnetic ring. The magnetic induction intensity in the working area is DT4C, 20 steel, and 45 steel from strong to weak. The experimental results are consistent with the simulation.

RhoGDI2 induced malignant phenotypes of pancreatic cancer cells via regulating Snail expression

Yi Bin,Hu You,Zhu Dongming,Yao Jun,Zhou Jian,Zhang Yi,He Zhilong,Zhang Lifeng,Zhang Zixiang,Yang Jian,Tang Yuchen,Huang Yujie,Li Dechun,Liu Qiuhua 한국유전학회 2022 Genes & Genomics Vol.44 No.5

Background: Rho GDP dissociation inhibitor 2 (RhoGDI2) has been shown to contribute to the aggressive phenotypes of human cancers, such as tumor metastasis and chemoresistance. Objective: This study aimed to assess the effects of RhoGDI2 on tumor progression and chemoresistance in pancreatic cancer cells. Methods: The expression of RhoGDI2 in pancreatic cancer cells was detected by Western blot analysis. Gain-of-function and loss-of-function approaches were done to examine the malignant phenotypes of the RhoGDI2-expressing or RhoGDI2-depleting cells. The correlation between RhoGDI2 and Snail was also analyzed. Results: Differential expression of RhoGDI2 protein in pancreatic cancer cell lines was identified. Gain-of-function and loss-of-function experiments showed that RhoGDI2 induced the malignant phenotypes of pancreatic cancer cells, including proliferation, migration, invasion, and gemcitabine (GEM) chemoresistance. The upregulation of RhoGDI2 stimulated the expression of Snail, resulting in the altered expression of epithelial marker E-cadherin and mesenchymal marker Vimentin, which were characteristics of the tumorigenic activity of epithelial-mesenchymal transition. The expression of RhoGDI2 and Snail was upregulated in clinical tumor samples, and higher expression of RhoGDI2 or Snail was significantly associated with poor patient survival in pancreatic ductal adenocarcinoma (PDAC). Conclusion: The findings indicated that RhoGDI2 promoted GEM resistance and tumor progression in pancreatic cancer and that RhoGDI2 might be a potential therapeutic target in patients with PDAC.