Helicobacter pylori와 대장균의 Shuttle Vector 개발

조명제,이우곤,이상룡,김경희,안영숙,김성희,김현주,류복덕,최여정,윤영혜,백승철,전영석,이광호 경상대학교 유전공학연구소 1997 遺傳工學硏究所報 Vol.16 No.-

In this study, a vehicle vector using cryptic plasmids was constructed for gene transfer in Helicobacter pylori. pHP51(3.9 kb) and pHP489(1.2 kb) were selected for constructing vectors from cryptic plasmid of H. pylori isolates in Korea. The HindⅢ-digestedDNA fragment(1.2kb) of pHP489 and 1.6kb DNA fragment of pHP51 were ligated with a kanamycin resistance gene(aph3'-Ⅲ) from C. jejuni to produce the recombinant plasmids pHP489K and pHP51K, respectively. Transformation frequency of pHP51K by electroporation was low. But pHP489K could be effectively transformed into various H. pylori strains. In order to design an intermdiate vehicle vector for gene transfer into H. pylori, pBlueHP489K was prepared by recloning pHP489K DNA into pBluescript and pTZ19R vector. This vector permitted the DNA fragment containing pHP489 sequence, aph3'-Ⅲ, and cloned DNA to be cut and self-ligated in the SacⅠ site after cloning. ureA and ureB gene were inserted into pBlueHP489K, resulting in pBlueHP489K/AB. The DNA fragment containing pHP489, kanamycin resistance gene(aph3'-Ⅲ), and urease structural gene was cut away from pBlueHP489K/AB and self-ligated to generate pBlueHP489K/AB. pBlueHP489K/AB made urease-negative H. pylori strains restore their urease activity. By this experiment, pBlueHP489K was confirmed to be the vehicle system for transferring H. pylori genes.

대장직장종양에 있어서 Hand-Assisted Laparoscopic Surgery (HALS)의 초기 경험

조호영,최선근,구지회,허윤석,이건영,김세중,조영업,안승익,홍기천,신석환,김경래,우제홍 대한내시경복강경외과학회 2003 대한내시경복강경외과학회지 Vol.6 No.-

MgCl₂溶液內에서 STS 304 HP Stainless Steel 熔接部의 破壞擧動에 對한 硏究

金永奭,丁太權,朴昌彦,洪錫柱,梁仁榮,金基玉,朴煥奎,李茂錫,曺圭宰,鄭在康 조선대학교 생산기술연구소 1983 生産技術硏究 Vol.1983 No.-

This paper is based on an experiment analysing the stress corrosion cracking behaviour of welding specimens compared to that of non-welding specimens of STS 304 HP stainless steel in corrosion solution. As a corrosion condition, stainless steel has been known to be the most fragile in a solution of 42% MgCl₂heated to the boiling point, therefore the experiment was carried out in this condition. The experiment was carried out with argon gas TIG welding specimens and non-welding ones made of stainless steel plates, thickness 2mm, by tensile force. At this time, the tensile force was taken to be the value of the yield strength of the tensile test specimens divided by the safety factor of 3.0 to 3.5, 4.0, 4.5, and 5.0. In this paper, the relations between Time of Initial Crack, Time of Failure, Time of Final Fracture and Crack Propagation Length were investigated and then plotted. Furthermore, the relations between Stress Intensity Factor K proposed by Irwin and the Time of Initial Crack, the Time of Failure, the Time of Final Fracture were calculated and plotted. At the last, all the relations plotted were expressed to normal equations by computer, and the equations were used to analyse the data obtained in the experiment. The conclusions obtained in this study are as follows: 1. Welding materials, compared with non-welding ones, as the Time of Initial Crack, the Time of Failure, and the Time of Final Fracture were generally very fast, appeared to be a phenomenon of unstable brittleness fracture. 2. Non-welding materials, compared with welding ones, as the safety factor was larger or the working stress was smaller, appeared to be a phenomenon that the longer had had greater durability. Therefor, welding materials must be considered enough to be used in heate affected and stress corrosion atmosphere. 3. The normal equations of the Time of Initial Crack and the variation rate to the same Stress Intensity Factor K are as follows: (A) Non-welding T =259702.4-5451.2 K+28.386 K² T = -5451.2+28.386 K (B) Welding T= -332.693+28.043 K-0.211 K² T' =28.043-0.211 K here, T; Time of Initial Crack, T'; Variation Rate of Time of Initial Crack to Stress Intensity Factor K, K ; Stress Intensity Factor.

Original Articles : Clinical significance of occult hepatitis B virus infection in chronic hepatitis C patients

( Jae Young Jang ),( Soung Won Jeong ),( Sung Ran Cheon1 ),( Sae Hwan Lee1 ),( Sang Gyune Kim ),( Young Koog Cheon ),( Young Seok Kim1 ),( Young Deok Cho1 ),( Hong Soo Kim ),( So Young Jin ),( Yun Soo 대한간학회 2011 Clinical and Molecular Hepatology(대한간학회지) Vol.17 No.3

Background/Aims: We investigated the frequency of occult hepatitis B virus (HBV) infection in anti-hepatitis C virus (HCV)-positive individuals and the effects of occult HBV infection on the severity of liver disease. Methods: Seventy-one hepatitis B virus surface-antigen (HBsAg)-negative patients were divided according to their HBV serological status into groups A (anti-HBc positive, anti-HBs negative; n=18), B (anti-HBc positive, anti-HBs positive; n=34), and C (anti-HBc negative, anti-HBs positive/negative; n=19), and by anti-HCV positivity (anti-HCV positive; n=32 vs. anti-HCV negative; n=39). Liver biopsy samples were taken, and HBV DNA was quantified by real-time PCR. Results: Intrahepatic HBV DNA was detected in 32.4% (23/71) of the entire cohort, and HBV DNA levels were invariably low in the different groups. Occult HBV infection was detected more frequently in the anti-HBc-positive patients. Intrahepatic HBV DNA was detected in 28.1% (9/32) of the anti-HCV-positive and 35.9% (14/39) of the anti-HCV-negative subjects. The HCV genotype did not affect the detection rate of intrahepatic HBV DNA. In anti-HCV-positive cases, occult HBV infection did not affect liver disease severity. Conclusions: Low levels of intrahepatic HBV DNA were detected frequently in both HBsAg-negative and anti-HCV-positive cases. However, the frequency of occult HBV infection was not affected by the presence of hepatitis C, and occult HBV infection did not have a significant effect on the disease severity of hepatitis C. (Korean J Hepatol 2011;17:206-212)

브로콜리 추출물의 향장활성 효과

김대용ㆍ조석철ㆍ권혁선ㆍ김미경(Dae Young KimㆍSeok chul ChoㆍHyuk sun KwonㆍMee Kyung Kim) 한국인체미용예술학회 2016 한국인체미용예술학회지 Vol.17 No.1

In this study, we investigated the anti-oxidative effects, antimicrobial, and anti-inflammatory activities of the extracts from broccoli to evaluate its use as a functional ingredient in cosmetics. The extracts are WE (water extract at RT), MWE (mineral water extract at RT), EE (70% ethanol extract at RT), and USEE (heat process for 12 hours at 60℃ after ultrasonification for six hours with 70% ethanol). The measurements of the total polyphenol content from broccoli extracts were highest in EE at 425.24±13.9 mg/g dry. The result of electron donating ability of EE was the highest at 10,000 ㎍/㎖ as 97.81% and increased in a concentration-dependent manner. The ferric reducing antioxidant powder (FRAP) of EE was the highest at 10,000 ㎍/㎖ as 1235.6 μM. In an antimicrobial activity test, the EE showed significant antimicrobial activity against S. epidermidis, S. aureus, E. coli, P. acnes, and P. ovale. Furthermore, broccoli extract exhibited no cytotoxicity in RAW 264.7 cells. Also anti- inflammatory activity by NO assay showed LPS-induced NO was significantly inhibited following treatment with EE of 1,000 ㎍/㎖. Therefore, the broccoli ethanol extract can be used as a resource of natural cosmetic material for functional cosmetics.

간세포암과 감별이 어려웠던 악성중피종 치험 1예

김선문,허원석,채경훈,강윤세,정재훈,김연수,박기오,문희석,이엄석,김석현,성재규,이병석,이헌영,신경숙,조준식,송인상,강대영 충남대학교 의학연구소 2003 충남의대잡지 Vol.30 No.2

Malignant peritoneal mesothelioma is a rare neoplasm that arises from the mesothelium of a serosal cavity and is a rapidly fatal disease with a median survival of 4 to 12 months for untreated cases. Recently, we experienced a case with malignant peritoneal mesothelioma who was suspected hepatocelluar carcioma by abdominal CT scan and was confirmed by biopsy including immunohistochemical stain(calretinin) after surgery. We performed tumor excisions and wedge resection of the liver(segment Ⅷ)and inserted Tencoff catheter in abdominal cavity at 25th day of post-operation. We treated with intraperitoneal paclitaxel(25mg/m^(2)/day for 5 days) six courses monthly. She was well tolerable and is still living without any evidence of recurrence for 14th month of post-operation.

Factors associated with the risk of colorectal neoplasia in young adults under age 40

Ilsoo Kim,Han Hee Lee,Young Jae Ko,Ho Eun Chang,Dae Young Cheung,Bo-In Lee,Young-Seok Cho,Jin Il Kim,Myung-Gyu Choi 대한내과학회 2022 The Korean Journal of Internal Medicine Vol.37 No.5

Background/Aims: Recent epidemiologic studies have shown a continued increase in colorectal cancer incidence among younger adults. Little is known about the factors that contribute to the development of young-onset colorectal neoplasia (CRN). Methods: A cross-sectional analysis was performed for individuals younger than 40 years who underwent colonoscopy in Seoul St. Mary’s Hospital and its affiliated health screening center. High-risk CRN was defined as adenoma or sessile serrated lesion ≥ 10 mm, with three or more adenomas, villous histology, high grade dysplasia, or carcinoma. Results: Of these 13,621 included participants, 2,023 (14.9%) had one and more CRN. Young patients with CRN tended to be elderly, male, obese, smoker, having a habit of drinking, and having comorbidities such as hypertension, dyslipidemia, diabetes, and chronic kidney disease. In a multivariate analysis adjusted for age, sex, obesity, smoking status, and alcohol intake, old age (odds ratio [OR], 1.086; 95% confidence interval [CI], 1.054 to 1.119), male sex (OR, 1.748; 95% CI, 1.247 to 2.451), obesity (OR, 1.439; 95% CI, 1.133 to 1.828), and smoking (OR, 1.654; 95% CI, 1.287 to 2.127) were independent risk factors for overall CRN. Obesity and smoking as two modifiable factors increased the risk for high-risk CRN even more than for overall CRN (OR, 1.734; 95% CI, 1.168 to 2.575 and OR, 1.797; 95% CI, 1.172 to 2.753, respectively). Conclusions: Obesity and smoking were modifiable risk factors for CRN in young adults. They increased the risk for highrisk CRN even more than for overall CRN. A colonoscopy might be beneficial for young individuals with these factors.

평균기울기법에 의한 Al 2024-T3합금의 X선 반가폭과 피로수명비의 관계

조석수,박승용,김득진,장득렬,주원식 東亞大學校 大學院 1998 大學院論文集 Vol.23 No.-

In order to predict the fatigue life fraction for Al 2024-T3, in-plane bending fatigue test and X-ray diffraction techniques was carried out. The relation between fatigue damage on the specimen surface and half-value breadth of X-ray diffracton lines was investigated in the process of cyclic loading. This paper proposed the estimation of fatigue life using AGM(Average Gradient Method) by the Gradient on the basis of B/Bo and log N/Nf.

LQ서보제어기를 이용한 천정크레인 시스템의 위치 제어

조영호,이한석,신승권,안두수 成均館大學校 科學技術硏究所 1998 論文集 Vol.49 No.1

An overhead crane is commonly used in factories and dockyards. An overhead crane operation is manually accomplished by skilled operators. Usually, the skill of the operators is relied on to bring the load to its destination exactly and have it stationary at the end of the journey. Recently, the concept of automation is widely introduced in shipping and unloading operation using the overhead crane for the enhanced producity. In this paper, we reduced overhead cranes scale and objection for experimental purpose in lab. The controller for an overhead crane was designed using LQ-servo technicques and simulated by MATLAB. The performance of the controller was verified through the experiment results and the simulation walls by MATLAB.

브랜드 개성이 브랜드 애착을 통해 구매의도에 미치는 영향에 관한 연구 : 화장품 브랜드 SK-II, ESTEE LAUDER, LANCOME을 중심으로

조영식;김준석 경희대학교 산업관계연구소 2011 産硏論叢 Vol.36 No.-

본 연구는 수입 화장품 브랜드가 가지는 브랜드 개성이 소비자와의 관계에 있어서 어떻게 그 브랜드 애착을 형성을 하는지와 브랜드 애착을 통해서 구매의도에 미치는 영향에 대해 실증적으로 조사하는데 그 목적이 있다. 연구를 위하여 각 화장품 브랜드 SK-II, ESTEE LAUDER, LANCOME 에 대해 총 270명을 대상으로 설문 조사를 진행하였다. 다중회귀 모형 분석에 의한 실증 분석 결과 첫째, SK-II와 ESTEE LAUDER의 브랜드 개성이 브랜드 애착에 대한 태도는 정(+)의 영향을 미치는 것으로 나타났다. LANCOME 은 유의미한 효과에만 미치는 것으로 나타났다. 둘째, 브랜드 애착이 구매의도에 대한 태도에는 SK-II, ESTEE LAUDER, LANCOME 모두 정(+)의 영향을 미치는 것으로 나타났다. 이러한 연구 결과는 브랜드 개성이 브랜드 애착관계를 통해서 구매의도에 영향을 줄 수 있다는 것에 의의를 찾을 수 있으며 향후 브랜드 개성이 기업 자산 가치의 성장에 중요한 영향을 주는 요소가 된다는 점과 브랜드 애착을 위해 브랜드 커뮤니케이션의 역시 고려되어야 할 중요한 요소임을 시사한다. The purpose of this research is empirically to investigate for How imported cosmetic brand personality establishes brand attachment with consumers and how the brand attachment influences toward purchase intention. In order to accomplish the purpose, the data was collected by total 270 peoples for SK-II, ESTEE LAUDER, LANCOME in cosmetic brand. Firstly, In terms of the attitude of brand personality toward brand attachment, SK-II and ESTEE LAUDER has shown positive result via multiple regression analysis. but LANCOME only determines to meaningful effect, as affect. Secondly, the brand personality toward the purchase intention, SK-II, ESTEE LAUDER and LANCOME all influenced to the effect of positive. Results of this study show that the impact of purchase intention through the strong linkage between brand personality and brand attachment. In addition, brand personality would be the critical factor or growth engine of the value of the company's assets therefore we need to closely monitor brand communication in order to manage brand attachment.