서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1998)

김재홍,김준호,반재용,이정우,황성주,정준규,정성태,강진문,조흔정,홍창의,정혜신,이한승,김이선,이봉길,이종호,선영우,한기덕,윤성필,이성훈,안종성,박석범,문승현,조항래,김형섭,류지호,황재영,박준홍,손상욱 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.1

In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria gonorrhoeae(PPNG), we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by menas of the chromogenic cephalosporin method. In 1998, 93 strians of N. genorrhoeae were isolated, among which 60(64.5%) were PPNG. The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 64.5% in 1998.

정면에서 평가한 한국인 여성 입술의 심미성과 전방 분절 골절단술 후 입술의 평가

이범석,강윤구,윤태호,국윤아 대한치과교정학회 2007 대한치과교정학회지 Vol.37 No.5

이 연구는 한국인 여자 유명 모델과 일반인의 정면입술의 차이점을 비교하여 아름다운 입술의 특징을 규명하며 양악 전방 분절골절단술을 시행한 환자에서 정면입술의 변화를 측정하고 그 수술 결과를 한국인 모델과 비교하여 차이점을 알아보고자 하였다. 한국인 유명 모델 30명, 일반인 26명과 양악 전방 분절골절단술을 받은 환자의 10명을 대상으로 정면입술 부위의 12개의 선 길이, 홍순의 5개의 각도와 둘레 및 면적을 측정하였고, 통계분석은 unpaired & paired t-test를 이용하였다. 한국인 유명 모델 군과 일반인 군의 비교에서 하안면 얼굴 폭경, 하안면 수직길이, 상홍순의 높이, 하순의 길이, 상홍순의 구각부 각도와 central bow의 각도는 의미 있는 차이로 일반인 군보다 유명 모델 군에서 작았다. 그러나, 입술의 폭, 하홍순의 높이, Cupid's bow tip간 거리와 하홍순의 구각부 각도는 유명 모델 군에서 컸고 입술의 둘레와 전체 면적에서도 일반인 군보다 컸다. 양악 전방 분절골절단술을 받은 환자의 정면입술에서는 하안면 얼굴 폭경, 상홍순의 높이, Cupid's bow tip에서 구각부까지의 길이와 상홍순의 면적이 통계적으로 의미 있는 변화를 보였고 한국인 유명 모델과의 비교에서 비슷한 수치를 나타내었지만 상순의 길이는 반대로 증가하였다. 연구의 결과는 교정치료 혹은 양악 전방 분절골절단술을 계획할 때 정면 입술 심미성을 평가하는 데 도움이 될 것으로 생각된다. Objective: The aim of this study was to determine firstly the characteristics of esthetic lips in Korean females and secondly to measure the changes of the lips before and after anterior segmental osteotomy in bimaxillary protruded patients. Methods: Samples consisted of 30 models and 26 nonmodels, and 10 patients who had received anterior segmental osteotomy. Twelve linear measurements, 5 angular measurements, and the lip perimeter and area were measured. These results were compared for each group using unpaired and paired t-tests. Results: Full face width, nose to chin, upper vermilion height and angle, lateral heights at the point of the tips of Cupid's bow, central bow angle, and the lower lip to chin lengths were significantly greater in nonmodels than in models. However, overall lip width, lower vermilion height and angle, tip-to-tip of Cupid's bow lip perimeter, and lower vermilion area were greater in models than in nonmodels. Comparison of before and after anterior segmental osteotomy revealed that the values for the upper vermilion and lateral heights, the angle to Cupid's bow tip lengths, and upper vermilion area of post-operative patients had become similar to those of Korean female models. Our findings demonstrated that Korean female models have a fuller lower vermilion & thinner upper vermilion compared with nonmodel controls, as determined by the vermilion heights, angles, and areas. Conclusions: It will be helpful for clinicians to use these measurements as guidelines for improving patients' facial esthetics.

다량재해에 있어 미토콘드리아 DNA의 다형성을 이용한 개인식별 : 삼풍백화점 사건에 대하여

이숭덕,신창호,김기범,최영태,이윤성,이정빈 內務部 國立科學搜査硏究所 1996 國立 科學 搜査 硏究所 年報 Vol.28 No.-

대량재해에 있어 미토콘드리아 DNA의 다형성을 이용한 개인식별

이숭덕,김기범,이윤성,최영태,신창호,이정빈 大韓法醫學會 1996 대한법의학회지 Vol.20 No.1

The mitochondrial DNA(mtDNA) is a small extranuclear DNA molecule that has been sequenced in man. the mtDNA has distinct characteristics over nuclear DNA. First, the evolutionary rate of nucleotide substitution appears to be larger compared with the nuclear DNA. Second, mtDNA is maternally inherited, so the determination of the various haplotype is unequivocal and no recombination has to be involved. Third, there is a 1.1 kb long control region, which shows severe polymorphism. These characteristics have been applied for several evolutionary study. Furthermore, they exist in cytoplasm with numerous copy number and their size, 16,569 bp is small relative to nuclear DNA, so they are more resistant to degradation or can be types in samples with only cytoplasm such as hair shaft without hair root. These presented the possibility of mtDNA as a tool in individual identification, especially when the nuclear DNA be unavailable. Actually, polymorphism in control region has been applied to individual identification for the skeletal remain found in Vietnam war. So we decided to apply the polymorphism of mtDNA in control region for the individual identification in case of mass disaster-"Downfall of Sampoong Department". Human remains from total 27 different individuals and 178 control persons were submitted for the individual identification. As the mtDNA comparison could be done through maternal lineage, 12 individual from 9 families from the control group were ruled out, because these families were consisted of father or were seeking for their father. Sequencing for the mtDNA control region was done using ABI automatic sequencer and DyeDeoxy Terminator Cycle Sequencing Kit. For convenience, sequencing reaction was done for two separate control region, region I from 16016-16401 in Anderson sequence, and regionⅡ from 048-388. First, sequences in regionⅡ of all samples and control persons were compared, and 20 samples were ruled out with no identical sequences in control group. Remaining 7 samples showed identical sequences with some of the control persons in three groups, and these identical pairs underwent mtDNA region I study. After region I study all samples except two dropped out in blood relationship. For the confirmation, STR and VNTR study such as MCT118, apoB, YNZ22, vWF, MBP, D21S11, TC-11 were done for remaining the two samples. The result denied the blood relationship between these two samples and any of the control group.

월남전 당시 살포된 고엽제에 의해 발생한 것으로 생각되는 피부 질환

이증훈,김윤동,서기범,박장규 충남대학교 의학연구소 2001 충남의대잡지 Vol.28 No.2

Although many dermatologists need adequate information about Agent Orange-related skin lesions, there have been few researches about them. We observed various skin manisfestations from 266 Korean Vietnam veterans who visited Department of Dermatology, Chungnam National University Hospital, inculding seborrheic dermatitis(46%), xerotic eczema(45%), chloracne(30%), generalized pruritus (10%), dermographism (5%), chronic urticaria (4%), photosensitive dermatitis (3%), etc. Among them chloracne is well known as a disease which results from exposure to Agent Orange which was used for a defoliating agent during Vietnam war. It is a refractory acneiform eruption due to halogenated polyaromatic compounds(eg. dioxin, dibenzofurane, polychlorinated biphenyls, etc.). 2,3,7,8-tetrachlorodibenzo-p-dioxin, a kind of dioxin isomer, contained in the Agent Orange which was used for a defoliating agent during Vietnam war. It is characterized by recalcitrant comedones and cysts predominantly in the malar and postauricular area. We concluded that the various skin lesions in Vietnam veterans resulted from probably the chemicals in defoliant and further researches would be required to find out subjective guidelines for the evaluation of the skin lesions.

콩에서 발생하는 강낭콩일반모자이크바이러스(BCMV)와 땅콩위축바이러스(PSV)의 저항성 평가

이영훈, 윤영남, 강범규, 김현영, 최만수, 구성철, 김현태, 윤홍태, 이수헌, 백인열 忠北大學校 農業科學硏究所 2014 農業科學硏究 Vol.30 No.2

It has been reported several viral diseases in soybean. The occurrence of Bean common mosaic virus and Peanut stunt virus are able to induce severe problem because the main breeding target for viral disease was focused on Soybean mosaic virus. BCMV is a spcies of the genus Potyvirus of the family Potyviridae. BCMV reduces yield by as much as 80% and also adversely affects seed quality in common bean. BCMV has a worldwide distribution because of its high rates 35% of transmission via seeds produced by plants systemically infected prior to bloom. PSV is a species of the genus Cucumovirus of the family Bromoviridae. PSV has been reported to be economically damaging on beans worldwide. Although the virus is reportedly seed transmissible at very low rates, infected plants rarely produce pods with normal viable seeds. The most satisfactory management method of viral diseases such as BCMV and PSV is the use of resistant cultivars and healthy seed. For the overcome of genetic resistance by new virus such as BCMV and PSV, virus diseases monitoring and researches need to study deeply and continually. In this study, we conducted the resistance screen on BCMV and PSV for selection of resistance cultivars. We inoculated on 128 cultivars with PSV and BCMV respectively. These cultivars were classified through pathogenic response such as resistance, necrosis and susceptibility. In addition, 99 and 66 resistant cultivars were confirmed against PSV and BCMV respectively.

제2형 당뇨병 환자에서 QUICKI법을 이용하여 측정한 인슐린 저항성과 비만도와의 관계

성윤경,최웅환,이창범,박용수 대한비만학회 2003 The Korean journal of obesity Vol.12 No.3

연구배경: 인슐린 저항성은 제2형 당뇨병의 중요한 병인으로서 비만과 밀접한 관계가 있으며, 이와 연관된 고지혈증. 고혈압의 발생은 결과적으로 혈관 합병증의 빈도를 높이는 것으로 알려져 있다. 본 연구에서는 한국인 제2형 당뇨병 환자를 대상으로 하여 새로운 인슐린 저항성 평가법인 QUICKI법을 이용하여 인슐린 저항성을 측정하고, 이와 비만도 및 체지방 분포, 지질 등의 대사성 인자들과의 상관 관계를 평가하고자 하였다. 방법: 연구 대상은 2000년 5월부터 2002년 10월까지 한양대학병원 내분비대사내과에서 당뇨병으로 치료 받은 환자 중 인슐린과 경구혈당 강하차를 투여하지 않은 103명을 차상으로 하였으며, 인슐린 저항성은 공복시 측정한 인슬린치와 포도당치를 이용하여 계산하는 QUICKI법을 이용하였다. 비만도는 입원시 키와 몸무게를 통하여 체질량지수를 측정하였으며, 체지방의 정량화와 분포를 알아보기 위하여QDR4500의 DEXA system (Hlologic, USA)을 이용하여 지방량과 건체중. 총 체지방률, 복부 지방률 등을 측정하였다. 측정 결과는 평균, 표준편차, 백분율 등으로 제시하였고 남녀간평균값의 비교 분석은 independent t-test를 시행하였다. 인슐린 저항성과 각 변수들과의 상관 관차는Pearson 상관 차수를 통해 비교하였고, 단계별 다중회귀분석을 통하여 인슐린 저항성에 가장 영향을 미치는 인자를 평가하였다. 이상의 모든 통계 분석은 SPSS (version 11.0)를 사용하였으며, 통계학적 유의 수준은 p값을 0.05 이하로 하였다. 결과: (1) 평균 연령은 55.7±12.8세, HbA_(IC)는 9.9±2.7%로 측정되었고, 체질량지수는 23.8±3.7kg/㎡', QUICKI값은 0.38±0.07로 계산되었다. (2) 남녀간에 체질량지수. EHA_(IC), 연령 등에서는 유의한 차이가 없었던 반면 QUICKI값. 총 체지방량 및 복부 지방량. 총 체지방률과 복부 지방률, 그리고 각각의 지질치에 있어서 유의한 차이가 있었다. (3) QUICKl법으로 측정된 인슐린 저항성은 HDL 콜레스테롤과 양호한 양의 상관 관계(r=0.456, p<0.01)를 보인 반면, 체질량지수(r=0.407, p<0.001), 총 체지방량(r=0.458, p<0.001), 복부 지방량((r=0.475, p<0.001), 총 체지방률(r=0.438, p<.0.01), 복부 지방률(r=0.457, p<0.01) 그리고 건체중과 대비한 총 체지방량(r=0.392, p<0.01)과 복부 지방량(r=0.423, p<0.01)과도 양호한 음의 상관 관계를 보였으며, 총 콜레스테롤(r=0.281, p<0.01), TG (r=0.333, p<0.01), LBL 콜레스테롤(r=0.326, p<0.01)과도 음의 상관 관계를 보였다. 그러나 수축기 및 이완기 혈압, HbA_(IC)와는 유의한 상판 관차를 보이지 않았다. 한편, 이러한 대사성 인자들과 QUICKI값과의 상관 관계의 정도는 HOMA_(IR)값과의 상관 관계에 비해 약간 높은 것으로 나타났다 (4) 단계별 다중회귀분석을 통하여 인슐린 저항성에 가장 영향을 미치는 인자를 분석한 결과 복부 지방률(R²=0.209, p<0.001), HDL 콜레스테롤(R²=0.135, p<0.001), 총 콜레스테롤 (R²=0.056, p<0.01) 등의 기여율이 가장 높은 것으로 나타났다. 결론: 한국인 제2형 당뇨병 환자의 인슐린 저항성을 QUICKI법을 이용하여 측정하였으며, 단순한 체질량지수나 전체 및 복부의 절대적인 지방량 보다는 복부를 구성하는 체성분중 지방이 차지하는 비율인 복부 지방률과 혈중HDL 콜레스테롤 및 총 콜레스테롤 수치가 인슐린 저항성을 가장 잘 설명하는 것으로 나타났다 따라서 이러한 인자들에 대한 개선 노력은 인슐린 저항성의 개선 및 제2형 당뇨병 발현의 억제에 기여할 것으로 판단된다. Background : Insulin resistance plays an important role in the pathophysiology of diabetes and is associated with obesity and cardiovascular disease. The aim of this study was to investigate the relationships between insulin resistance generated by quantitative insulin sensitivity check index(QUICKI) and regional adiposity, body mass index (BMI), and serum lipid profile in Korean type 2 diabetic patients. Method: Study subjects included fat mass and lean body mass by dual energy x-ray absorptiometry(DEXA) system. Insulin resistance was estimated by the QUICKI. Result :50 were women and 53 were men, with a mean (±SD) age of 54.2 years(±12.8). The mean(±SD) score of QUICKI was 0.38(±0.07). Insulin resistance was positively correlated with total body fat percent, abdominal fat percent, BMI, total cholesterol, LDL-cholesterol, and triglyceride, and negatively correlated with the HDL-cholesterol. In multivariate model, the predicting variables on insulin resistance were abdominal fat percent, HDL cholesterol, and total cholesterol. Conclusion: In this study, we observed that the abdominal fat percent, HDL cholesterol and total cholesterol were meaningful variables correlated with insulin resistance among variables. Therefore, to improve insulin sensitivity in Korean type 2 diabetic patients, we should reduce the abdominal fat percent and total cholesterol and increase the level of HDL-cholesterol.

진동에 의한 혈관 내 폐 보조장치의 산소전달 특성

김기범,권대규,이삼철,김성종,정인수,오인혁,김기주,변윤섭,정경락 한국화학공학회 2004 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.42 No.2

본 연구는 급성호흡부전환자를 치료하기 위하여 사용되고 있는 혈관 내 폐 보조장치의 기체전달을 향상시키기 위하여 중공사막에 진동기법을 사용하여 기체전달을 향상시키고자 시도하였다. 그리고 혈관 내 폐 보조장치를 정맥에 삽입하기 전, 혈관 내 폐 보조장치의 설계조건을 실험적 모델을 통하여 기체전달을 예측할 수 있는 예측식을 만들고자 하였다. 실험결과, 본 연구에서는 진동기법이 기체전달을 향상시키는데 효과적임을 확인할 수 있었다. 뿐만 아니라, 충진율과 가징 주파수의 함수관계를 이용하여 기체전달을 예측 할 수 있었다. 실험에 의하여 얻어진 결과는 예측식에 의하여 얻어진 결과와 유사한 결과를 얻었다. 그러므로 본 연구에서는 충진율과 가진 주파수의 함수를 이용하여 VIVLAD의 기체전달을 예측할 수 있었다. In this paper, we tried to improve gas exchange of the vibrating intravascular lung assist device(VIVLAD) using vibrating method in the hollow fiber membrane, for the patients suffering from acute respiratory distress syndrome(ARDS). And we tried to formularize prediction equations to make a prediction about gas transfer for designing intravenous artificial lung assist device, and designed modules under various conditions were studied through an experimental modeling before inserted the artificial lung assist device into as venous. As a result, we are convinced that vibration method is very useful for the has transfer increasing. Also, we can estimate the gas transfer as a function of the packing density and excited frequencies. The gas transfer obtained from the experiment was similar to that from the prediction equation, confirming the usefulness prediction equation. Therefore, we can estimate the gas transfer of the VIVLAD as a function of the packing density and excited frequencies.

랫드의 화상모델에서 전복껍질과 참기름의 치료효과

남상윤,이범준 한국수의공중보건학회 2003 예방수의학회지 Vol.27 No.2

This study was performed to investigate effect of a mixture of ear shell ash and sesame oil on bum in a Sprague-Dawley rat model. Sixty male rats were assigned to 3 experimental groups including negative and positive control groups, and a test material group. The test compound was applied to formally once a day f3r 7 days. Distilled water was applied as a relative positive control. After dermal applications f3r 3, 5, and 7 days, blood hematology and skin histopathological examination were performed. Inorganic compounds in ear shell ash was mainlycalcium oxide(92%). The body weights of positive control rats were significantly low at day 3, 5, and 7 as compared with negative control and test material groups. There was no significant difference in body weight between negative control and test material groups throughout the experimental period. WBC counts in both positive control and test material groups were higher than those in negative control group, indicating progress of inflammatory reaction. In differential leucocyte count, panulocytes including neutrophils and eosinophils were higher in positive control and test material groups than negative control group. Hemoglobin and hematocrit values were lower in homing model rats, probably resulting from hemorrhage in dermis. Skin histopathology showed severe full thickness coagulative necrosis in epidermis and dermis, hemorrhage and infiltration of leucocytes in dermis in both positive control and test material groups throughout the experimental period. These results indicate that the test materials, ear shell ash plus sesame oil, moderately ameliorate homing probably concerning to process of skin injury repair including inflammation and fibrosis

조갑-슬개골 증후군 1예

김범준,윤호준,원종현,이종희,권오상 대한피부과학회 2006 大韓皮膚科學會誌 Vol.44 No.4