서울의 Penicillinase Producing Neisseria Gonorrhoeae 발생빈도(1996)

김재홍,황동규,전재홍,김윤석,김중환,김용준,이창균,임동진,김현수,조창근,김경문,박상훈,전우형,김희성,이호정,차명수,김갑형,김형석,김석우,황지환,박병순,권오상,이민수,송기훈,성소영,이인섭,부태성 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.2

Background : In recent years, gonorrhea has been panedemic and remains one of the most commom STDs in the world, especially in developing countries. Objective & Methods: For the detection of a more effective therapeutic regimen and assessing the prevalence of PPNG, we have been trying to study the patients who have visited the VD Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results: In 1996, 139 strains of N. gonorrhoeae were isolated, among which 53(39.0%) were PPNG. Conclusion: Our results suggests that after a peak of 74.3% in 1993, the prevalence of PPNG in Seoul is gradually declining.

신경섬유종증 환자에게서 발생한 특발성 혈소판 감소증 1예

권세훈,김형호,강지인,장재혁,원경준,장재현,김상용,배학연 朝鮮大學校 附設 醫學硏究所 2006 The Medical Journal of Chosun University Vol.31 No.3

Idiopathic thrombocytopenic purpura is a disease characterized by thrombocytopenia in peripheral blood with positive anti-platelet antibody titer, no splenomegaly, the presence of megakarocyte. The causes of idiopathic thrombocytopenic purpura were usually related to autoimmune disease, infection and drugs. A 46-years-old man was admitted for evaluation of abdominal mass. He has many cafe-au-lait spots and nodules in almost skin for 30 years. Several years ago, he palpated mass in Lt. inguinal area. hIe has known the abdominal mass after checking abdomen CT in local clinic and visited our hospital for evaluation of abdominal mass. At admission, laboratory tests showed hemoglobin 12.5 g/dL, hematocrit 38.4 %, WBC 5,460 /㎣ (neutrophil 64.5%, lymphocyte 21.7%, monocyte 4.1%, eosinophil 5.4%), reticulocyte 0.7%, Platelet 52,000 /㎣, total protein 6.51 g/dL, albumin 3.80 g/dL, alkaline pohsphatase 161 IU/L, AST 110.7 IU/L, ALT 64.9 IU/L, total bilirubin 0.44 ㎎/dL, BUN 18.4 ㎎/dL, creatinine 0.79 ㎎/dL. Peripheral blood smear showed normocytic normochromic anemia, thrombocytopenia. Bone marrow aspiration smear showed normoplastic myeloid and erythroid precursor. The bone marrow biopsy section shows normocellular marrow (35%) for his age and mildly increasing myeloid cell lineage. We have experienced a case of idiopathic thrombocytopenic purpura in a patient being in neurofibromatosis and therefore reporting it with documentary records. 저자들은 신경섬유종증 환자에서 특별한 원인을 발견할 수 없었음에도 특발성 혈소판 감소증이 발생한 1예를 경험하였기에 문헌고찰과 함께 보고하는 바이다.

Film중감지와 Film의 활용에 따른 화질특성의 고찰

권용재,이상모,김순희,김도형,최태균 한국건강관리협회 2000 건협 Report Vol.3 No.-

호중구감소증 환자에서의 기관-기관지 아스페르길루스증 1예

권재철,송명준,김기조,오유경,김성용,박선희,최수미,이동건,이석,신완식,김춘추 대한감염학회 2004 감염과 화학요법 Vol.36 No.1

저자들은 급성 림프구성 백혈병 환자에서 항암치료 후 호중구감소증이 지속되는 상태에서 침습성 아스페르길루스증의 드문 형태인 치명적인 아스페르길루스 기관기관지염을 경험하고 문헌고찰과 함께 증례를 보고하는 바이다. Tracheobronchial aspergillosis is an uncommon clinical form of invasive aspergillosis, particularly found in patients with AIDS and in lung transplant recipients than in other immunocompromised patients. The rapidity of the disease progression can result in fatal airway obstruction in a short period of time that the patient may need emergency tracheostomy without knowing the cause of the obstruction. We describe a case of fatal tracheobronchial aspergillosis which developed in a 43-year-old female patient with acute lymphoblastic leukemia. Dyspnea and stridor developed on the 20th day after 2nd consolidation chemotherapy, which was during the prolonged neutropenic period. Airway narrowing was observed on the computed tomograph scan of neck and emergency tracheostomy was performed. Infiltration with aspergillus hyphae was found in the tracheal ring and bronchial mucosa. Despite the use of amphotericin B, the patient died of bleeding and airway obstruction.

국내 살모넬라 분리주에서 spvR 유전자 분포와 단일 뉴클레오티드 다형 연구

권혁준,박경윤,박주연,박용호,김선중,유한상 대한감염학회 2004 감염과 화학요법 Vol.36 No.6

목적 : 지금까지 병원성 살모넬라를 확인하기 위해 분자량이 큰 플라스미드의 존재 유무를 확인하여 왔으나 살모넬라 혈청형 및 검출 방법에 따라 양성율에 큰 차이를 보이고 있고, 일부 병원성 살모넬라 분리주에서 병원성 플라스미드가 관찰되지 않아 spv 유전자들의 유무에 대한 아른 확인방법이 필요하다. 본 실험은 국내 동물 유래 주요 살모넬라 혈청형에서 spvR 유전자의 분포를 알아보고, 병원성 살모넬라 분자진단을 위한 분자표지로써의 가치를 평가하기 위해 실행하였다. 재료 및 방법 : 국내 가축 유래 S. lyphimurium (ST, 26주), S. enteritidis (SE, 10주), S. pullorum (SP, 40주), S. gallinarum (SG, 53주)에 대해 GenBank에 등록된 ST, SE, SP의 spvR 유전자를 비교하여 ST와 SE 간에 차이를 보이는 single nucleotide polymorphism (SNP, 625번 뉴클레오티드)을 포함하도록 시발자를 합성하여 집락-PCR을 수행하였다. 증폭 산물(194bp)은 자동 염기서열장치를 이용하여 염기서열을 결정하였고, 제한효소인 MseI을 사용하여 PCR-RFLP를 실시하였다. 결과 : 집락-중합효소연쇄반응 결과 SP, SG, SE의 모든 (100%) 분리주에서 특이 증폭산물이 검출되었으나 ST의 경우 19주(73%)에서만 증폭되었다. 특히 병원성 관련 플라스미드가 관찰되지 않았던 SP 4주에서 특이적인 증폭산물이 검출되었다. 염기서열 분석결과 SE, SG, SP는 625번 뉴클레오티드에 아데닌을, ST는 구아닌을 가지고 있어 PCR-RFLP를 이용하여 쉽게 구분할 수 있었다. 결 론 : spvR에 대한 집락-중합효소연쇄 반응법은 병원성 플라스미드 검출법보다 SE, SP, SG 병원성 주를 신속하게 검출하는데 유용하고, 625번 뉴클레오티드의 SNP는 ST와 SE, SG, SP를 구분하는 분자표지로 사용될 수 있을 것으로 기대되며, ST에 비해 SE는 진화적으로 SG와 SP에 가까운 것으로 생각되었다. Background : The Salmonella virulence plasmid (spv) genes (spvR, A, B, C and D) on the large virulence plasmids of pathogenic Salmonella serotypes can replace the virulence of the whole plasmid. Recently, virulence plasmid-negative pathogenic Salmonella isolates were isolated. However, positive rates of spv genes among Korean Salmonella serotypes have been obscure. spv genes are conserved in compared to other virulence genes but there are single nucleotide polymorphisms (SNPs) conserved in only certain serotype. Such SNPs are useful for differentiation and understanding evolution of certain serotypes. Materials and Methods : Salmonella serotypes isolated from live stocks [Salmonella typhimurium (ST, 26), S. enteritidis (SE, 10), S. gallinarum (SG, 40) and S. pullorum (SP, 53)] were used for colony-PCR. A primer set covering single nucleotide polymorphism (SNP) at 625th nucleotide of spvR was designed. The nucleotide sequences of amplicons were determined by cyclic sequencing method and RFLP was performed by using MseI. Results : All isolates of SE, SG and SP, including four plasmid-negative isolates, showed specific amplicons but not all of ST (19/26, 73%) were positive to spvR. Based on the nucleotide sequence of 625th nucleotide and PCR-RFLP, SE, SG and SP [A(625)] and ST [G(625)] could be differentiated. Conclusion : spvR can be used as a molecular marker to detect virulent SE, SG, SP and the SNP may be useful for differentiation of SE, SG, SP and ST. According to the SNP study SE may be evolutionarily closer to SG and SP than ST.

葡萄(Campbell Early)의 結實에 미치는 GA 및 覆袋의 影響

權相洙,具禹書,朴正璂,權五昌,李容門 東亞大學校 1983 東亞論叢 Vol.20 No.3

葡萄 Campbell Early 의 無核果 生成 및 成熟促進을 위하여 GA₃ 濃度別 그리고 몇 가지 覆袋處理를 한 實驗結果는 다음과 같다. 1. 果房重과 果粒數는 滿開 8日前 處理가 適期로서 果房重은 無處理에 비해 76.4%, 果粒數는 80.6%였다. 2. 1果房當 無核粒率(92%)과 100粒中 種子數(12個)는 滿開 18日前, Gibberellin 處理+cellophane紙 覆袋區가 가장 良好하였다. 3. 果粒數와 種子數(r=0.826) 또한 種子數와 果重間에는 정의 相關關係(r=0.841)를 나타내었다. 4. 糖度는 無處理區보다 cellophane紙 覆袋區가 9.34% 로서 높았다. 含水量과 灰分은 無處理와 新聞紙 覆袋區가 높았고 cellophane 紙 覆袋區가 낮았다. 5. vitamin C 含量은 Gibberellin 處理時期가 빠르고 無核率이 높을수록 낮은 傾向이엇다. 處理間에 있어서는 無處理와 parchment紙 袋覆區 많았고 cellopnane紙 袋覆區가 含量이 적었다. 6. Gibberellin 處理時期를 앞당기고 cellophan紙 袋覆를 하면 無核率이 높은 反面 果粒數 果重이 減少되고, Gibberellin 處理時期를 늦추고 新聞紙 覆袋를 하면 果粒數 果重은 增加하지만 無核率이 낮아졌다. The results obtained by the Gibberellin and bagging treatment on the Campbellearly grape are as follows: 1. The suitable period of treatment for cluster weight and number of berry are to be before 8 days of full bloom. The cluster weight was 76.4% and number of berries were 80.6% compared with control. 2. The ratio seedless berries of 92% per cluster, and number of 12 seedss in 100 beries were showed the best on before 18 full bloom at the bagging plots of Gibberellin treatment+cellophane paper. 3. Number of berry and number of seed (r=0.826) also were showed mutual relation of plus between the number of seed and fruit weight. 4. Soluble solid was showed higher of 9.3% at the cellophane paper bagging plot and 9.34% at the parchment paper bagging plot than the control. Juice content and ash were showed high at the conthol and newspaper bagging plot, and low at the cellophane paper bagging plot. 5. The content of vitamin C was showed low tendency at the higher of the seedless ratio with the quick Gibberellin treatment period. At the betweentreatment, the control and parchment paper bagging plot were showed much, and showed less content at cellophane paper bagging plot. 6. In advance the period of Gibberellin treatment and had cellophane paper bagging then the ratio of seedless would be high, but on the contrary, the number of berry and fruit weight decreased, and had delay the period of Gibberellin treatment and had the newspaper bagging, the number of berry and fruit weight would be increased but the ratio of seedless was low.

방사선 조사선량에 따른 백서 악하선의 caspase-3 발현양상

권기정,최용석,황의환,이상래,고광준 대한구강악안면방사선학회 2006 Imaging Science in Dentistry Vol.37 No.1

Purpose : To investigate the caspase-3 expression in the acinar and ductal cells of rat submandibular glands after the irradiation of various doses. Materials and Methods: The male Sprague-Dawley rats weighing approximately 250 gm were used for this study. The experimental group was irradiated with a single absorbed dose of 2, 5, 10, and 15 Gy on the head and neck region. The rats were sacrificed on the 1st, 3rd, 7th, 14th, 21st, and 28th day after irradiation. The specimens including the submandibular gland were sectioned and observed using histopathological and immunohistochemical methods. Results : The local destruction of the acinar and ductal cells and the karyopyknotic nuclei of the acinar cells were observed in the 2 Gy and 5 Gy irradiation groups later than in the 10 Gy and 15 Gy irradiation groups. And the expression of caspase-3 was prominent only in the ductal cells in the 2 Gy and 5 Gy irradiation groups. Conclusion : This experiment suggests that radiation-induced apoptosis in the ductal cells of rat submandibular glands was induced by a low dose radiation associated with the activation of caspase-3 and radiation-induced necrosis was induced by a high dose radiation. (Korean J Oral Maxillofac Radiol 2006; 36 : 7-15)

유전체 공진기를 이용한 개인휴대 통신용 대역통과 필터의 설계 및 구현

권정열,이헌용,홍성용,윤중락 明知大學校 産業技術硏究所 1996 産業技術硏究所論文集 Vol.15 No.-

In this paper, the band pass filter for personal communication services was constructed and designed using λ/4 TEM mode dielectric resonators with a (Zr0.65,Sn0.35)ATi1.04O4.04 ceramic of dielectric constant εr=37.8, Qxfo=48.600 temperature coefficient of resonant frequency rf=7ppm/˚C, and Unloaded-Q (Qo) of λ/4 TEM mode dielectric resonator with inner hole size 0.9㎜ and external size 3㎜ using silver electrode is 354.5 at 1950MHz. For the band pass filter design and construction, the design theory and simulation results of band pass filter using J-inverter theory have been studied. The parameters which are evaluated by design theory are practically applied to the filter construction and the simulation results are in agreement with the measured results after fine tunnings.

말초혈액에서 Tg mRNA에 대한 역전사 중합효소 연쇄 반응법의 갑상선 재발암의 분자생물학적 진단

권성일,박기룡,김현영,신채희,임영찬,최영식,박요한,이강대,장희경,이재화,염하용 대한내분비학회 2002 Endocrinology and metabolism Vol.17 No.4

연구배경: 갑상선암은 다른 조직에 발생한 암에 비해 비교적 천천히 자라므로 대부분 예후가 양호하지만, 일부에서는 주위 조직으로 침윤하거나 혹은 원격 전이로 인하여 치명적인 결과를 초래할 수 있다. 갑상선전절제술 및 131^I 제거술 후 경과 관찰시 갑상선암의 재발과 전이의 진단에 있어 131^I 스캔과 혈청 Tg의 측정이 현재 임상에서 가장 많이 이용되고 있으나 이 방법에는 여러 가지의 결점이 있다. 최근 Tg mRNA에 대한 RT-PCR법을 이용한 여러 연구결과는 131^I 스캔과 혈청 Tg 측정의 결점을 보완할 수 있는 좋은 보조적인 진단법으로 이용할 수 있을 가능성을 제시하였다. 이에 말초혈액에서 측정한 Tg mRNA에 대한 RT-PCR법이 갑상선 절제술 및 방사성요드 치료 후 갑상선암의 재발 및 전이 유무의 진단에 유용한가를 알아보고자 이 연구 시행하였다. 방법: 분화된 갑상선암으로 진단된 후 갑상선전절제술을 시행받고 방사성요드 치료를 받은 환자 중 현재까지 한차례에 이상 추적 방사성요드 전신 스캔을 시행하고 추적관찰이 가능했던 유두선암 35예, 여포선암 5예를 대상으로 연구를 시행하였다. 대상군은 131^I 스캔 소견상 음성인 군(Group Ⅰ), 잔여조직이 있는 군(Group Ⅱ), 국소전이가 있는 군(Group Ⅲ), 및 원격전이 군(Group Ⅳ)으로 구분하였다. 정상 대조군은 갑상선질환이 없는 10예의 건강인으로 하였다. 대상환자의 말초혈액을 이용한 Tg mRNA에 대해 특이적인 primer를 이용하여 RT-PCR 및 nested RT-PCR을 시행하였다. 결과: 본 연구 결과는 다음과 같다. 1) 131^I 스캔 소견상 음성인 군 21예 중 1예에서 Tg가 양성소견을 보였다. Anti Tg Ab가 양성인 4예 모두 Tg가 음성을 보였다. 잔여조직이 있거나 국소전이 및 원격전이를 보인 군 19예 중 Tg가 양성인 경우는 4예였으나, Tg mRNA는 전예에서 양성이었다. 2) 131^I 스캔에서 국소 및 원격전이 소견을 보인 8예 중 4예에서 Tg가 음성으로 131^I 스캔과 혈청 Tg 사이에 불일치 소견을 보였다. 3) 말초혈액에서 특이적인 primer를 이용하여 RT-PCR 및 nested RT-PCR을 시행한 결과 대상군 40예 및 정상 대조군 10예 모두에서 Tg mRNA가 양성을 보였다. 결론: 본 연구에서 갑상선 절제술 및 방사성요드 치료 후 갑상선암의 재발 및 전이 유무를 평가함에 있어 역전사 중합효소 연쇄 반응법을 이용한 Tg mRNA 측정의 의의는 재평가되어야 한다고 생각된다. Background: Differentiated thyroid cancer is the most common endocrine malignancy. Despite advances in the treatment of thyroid cancer, disease recurrence and metastasis may occur in as many as 20% of patients, and so continues to pose major problems in its clinical management. Serum thyroglobulin (Tg) measurements, by immunoassay, are used to detect residual or recurrent thyroid cancer following thyriod ablation. However, the usefulness of immunoassay is limited by both the requirement for thyroid hormone withdrawal, to attain optimal test sensitivity, and interference by the antithyroglobulin antibody (Anti-Tg Ab). Recent studies have reported the clinical usefulness of reverse transcription-polymerase chain reaction (RT-PCR) detection of Tg mRNA in the peripheral blood of patients with differentiated thyroid carcinomas. We performed this study to evaluated the usefulness RT-PCR of Tg mRNA in peripheral blood of patients with thyroid carcinoma following a total thyroidectomy and radioiodine ablation therapy. Methods: Forty cases that underwent a total thyroidectomy and radioiodine ablation therapy were included in this study. Of the 40 patients, 35 were papillary carcinomas and 5 were follicular carcinomas. Ten normal control subjects were also studied. Tg mRNA was extracted. Then RT-PCR and nested RT-PCR, were run with specific Tg primers. Concurrently, DNA sequencing of the isolates was carried out to prove the isolates were identical to the nucleotide sequence of the Tg. Results: The Tg was detected in 4 of 19 patients, with either a residual thyroid bed, or metastasis, on a 131^I whole body scan and in 1 of 21 patients with a negative radioiodine scan. Surprisingly, the Tg mRNA was detected in all the patients and normal controls. Conclusion: From our results we can not recommend Tg mRNA, detected by RT-PCR in peripheral blood, as a tumor marker superior to that of the Tg serum level. We consider an intensive re-evaluation of the method is required before considering its clinical applications (J Kor Soc Endocrinol 17:501∼513, 2002).

Higenammine이 심근의 활동전압 및 수축에 미치는 영향

권평중,조성일,엄대용,이상돈 중앙대학교 의과대학 의과학연구소 1981 中央醫大誌 Vol.6 No.4

In order to investigate the action mechanism of higenamine on cardiac muscle cells, the isometric contraction and transmembrane action potential in rabbit papillary muscle preparations taken out from tight ventricles were observed. The isometric contraction and action potential were recorded in the control Tyrode solution and higenamine-Tyrode solution in which the preparations were kept beating (1 Hz.) for about 30 minutes to get stable data. The resting membrane potential, amplitude of action potential amplitude of overshoot potential, time of repolarization to 60%, 90% of the amplitude of action potential and the first derivatives of potential recorded transmembrane action potential in control Tyrode solution and higenamine (2.5×10^-7gm/ml) Tyrode solution in which showed about 130%-150% of positive inotropic effect compared with those controls. The following results were obtained. 1. Higenamine showed no effects on the time-to-peak tension. 2. Higenamine showed almost no effects on resting membrane potential, amplitude of action potential and overshoot potential. 3. Higenamine significantly shortened to repolarize 60% or 90% of the action potential from 218ms and 264ms in the controls to 200ms and 246ms respectively. (p<0.005) Therefore. the maximum values of the first derivatives of potential variations (- Vmax) in the plateau phase an d repolarization phase were also significantly increased from 16.3V/sec in the control groups to 17.3V/sec in the higenamine-treated groups. The above results which showed positive inotropic effect and simultaneously shortening of action potential duration by higenamine suggest possible involvement of the increase of slow inward current.