형광검출 역상 액체크로마토그래피에 의한 Histamine의 정량

유희춘,김형룡,김상현,김대기,이영미,김형민,안년형,신태용 우석대학교 의약품개발연구소 1996 藥學硏究誌 Vol.1 No.-

Histamine을 정확하고 신속하게 정량하기 위해 9.Fiuorenylmethyl chloroformate를 형광유도체화제로 하여 역상 HPLC법으로 정량하였다. 히스타민을 형광유도체화할 때 반응액의 pH, 반응시간, 형광유도체화제의 농도 등 최적 반응 조건을 검토하였다. 이 방법으로 히스타민을 분석한 결과 0.1~0.5ug/ml의 농도범위에서 상관계수가 0.922인 양호한 직선성을 나타내었으며 검출한계는 0.01??/ml였다. A rapid and simple method for the determination of histamine by reverse-phase high performance liquid chromatography with fluorescence detection was established. 9-Fluorenylmethyl chloroformate(FMOC) was used as fluorescent derivative reagent. The optimum conditions for the derivatiation such as pH, reaction time and he concentration of FMOC were investigated. Linearity of calibration curve was obtained between 0.1ug/ml/and 0.5ug/ml(r=0.922) and the limit of detection was 0.01ug/ml.

키토산과 견사 단백질 복합체의 특성

김희숙,원용돈,류병호 한국식품영양학회 1996 韓國食品營養學會誌 Vol.9 No.4

기능성 다이어트 식품을 개발을 목적으로, 키토산과 견 피브로인의 복합체를 만들고 성질을 조사하였다. 키토산은 대동새우로부터 추출하였고, 견사 피브로인 섬유는 누에로부터 추출하였다. 견사의 특성을 알아보기 위하여 용해도를 조사한 결과, 용해도는 8M LiBr이상의 농도와 40℃ 이상에서 가장 높았다. 견사 피브로인의 아미노산 조성은 천연견사 및 용해시킨 피브로인과 같았다. 키토산, 견사 피브로인 복합체의 아미노산 조성은 glycine, alanine, serine, tyrosine, threonine 및 glutamic acid 순서를 많았다. 또한 IR 스펙트럼 결과, 키토산·견사 피브로인 복합체는 배합비율에 따른 스텍트럼상의 차이는 거의 없었다. In order to develop the functional diet food, characteristics of chitosan and silk fibroin composite was designed. Chitosan extracted from a prawn and silk fibroin was prepared from silkworm. The silk fibroin was dissolved rapidly in the 8M LiBr at a temperatrue of more than 40℃. Amino acid composition of fibroin composite revealed the same pattern that of native silk fibroin and regenerated silk fibroin. Predominant amino acid of chitosan-fibroin composite contained glycine, alanine, serine, tyrosine, threonine, and glutamic acid in order. According to the basis on the infrared spectrum, chitosan-silk fibroin composite is not distinguished differents composite ratio of chitosan and silk fibroin.

유입 바베시아증 1예

우준희,조용균,김은옥,채종일,김양수,양성연,유지소,이성순 대한감염학회 1997 감염 Vol.29 No.1

최근 외국으로의 여행이 빈번해지면서 유입 감염되는 경우가 많아지고 있는 추세이며 임상 증세가 말라리아와 유사하여 다소 진단이 어려울 수가 있어 한번쯤 바베시아증을 생각하는 것이 바람직하겠다. 저자들은 아프리카에 다녀온 뒤 나타난 바베시아증을 경험하였기에 문헌고찰과 함께 보고하는 바이다. Babesiosis is a tick-borne, malaria-like illness caused by Babesia species that infect erythrocytes of mammals incidentally. The family Babesiidae is characterized by consisting of nonpigmented intraerythrocytic parasites that reproduce within erythocytes by asynchronous, asexual budding into two or four daughter cells (tetrad). We experienced a case of human babesiosis presenting fever and chills. The patient was a 49-year old man, who had been in Africa (Ethiopia, Uganda). Three weeks before admission intermittent spiking fever had developed, which had been accompanied by severe chills. The peripheral blood smear (Giemsa-stain) revealed characteristic forms of a intracellular quadraple parasite compatible with Babesia. The patient was improved significantly by the treatment with quinine and clindamycin for a week.

저자계측정을 위한 종형 Hall 소자의 교류구동 원리

송명희,김정헌,박용우,류지구 경북대학교 센서기술연구소 1998 센서技術學術大會論文集 Vol.9 No.1

In this paper, the vertical Hall device which is sensitive to the magnetic fields parallel to the surface of the chip, is fabricated and characterized. The Hall voltage was ±0.2[mV] in the range of ±100[Gauss]. The current-related Magnetic sensitivity(S_(H)) was about 55[V/AT]. With an increased I_(sup), the offset voltage was also increased. Alternating Current(AC) driving system was used to process the amplification of Hall voltage and the reduction of offset and noise by the AC driving of the vertical Hall device. When the AC driving system was operated, the output of the Hall voltage was ±74.0 [mV] in the low magnetic field ranges of ±100 [Gauss], The magnetic fields resolution was 0.74[mV/Gauss].

구내신연장치를 이용한 치조골증대술 후 임프란트 매식 : 치험 5예 REPORT OF 5 CASES

이용욱,박충열,송종운,박홍주,김영운,오희균,유선열 대한악안면성형재건외과학회 2002 Maxillofacial Plastic Reconstructive Surgery Vol.24 No.2

Successful alveolar ridge augmentation by distraction osteogenesis method has been reported previously. There are several types of devices for alveolar ridge augmentation. In this paper, a new intraoral distraction device was used on the severe atrophic mandible in order to distract an alveolar ridge vertically in 5 patients. Mean amount of distracted alveolar ridge was 7.4㎜ (5∼9.5㎜). Dental implants were placed in the distracted alveolar bone and good osseointegration was obtained in all patients. Mean follow up period is 2years 6months (7months∼4years 9months). No complications, such as infection or resorption was observed in all patients. Alveolar ridge augmentation by gradual distraction seems to be a safe, simple and reliable procedure.

傳統家屋의 收藏空間에 關한 硏究 : 江原道文化財 指定家屋을 中心으로

김희준,유승용 강원대학교 조형예술연구소 2001 조형예술논문집 Vol.3 No.1

Traditional houses for the paper have been researched through storage space, an element of a traditional houses, in a different way of the studies which have been mainly researched into mail space of traditional houses. Species, number, size and site of each storage space have been analysed according to the life environment. The number of house for the research is 47, and all the houses have storage space and are the houses registered as cultural properties. The changes of storage space have been also examined, dividing before and after 1800.

절편모충(Trichomonas vaginalis)에 대한 단일클론항체의 생산과 그 특성

임미혜,류재숙,안명희,최영길,민득영 한양대학교 의과대학 1999 한양의대 학술지 Vol.19 No.1

Trichomonas vaginalis, mucosal protozoan parasite of the urogenital tract, is responsible for the most common clinically recognized sexually transmitted disease. Surface antigen of parasitic protozoa play the important roles in disease pathogenesis by serving as attachment factors, direct virulence factors, or mediating evasion of the host immune response through direct suppressoin or antigenic variation. In this study seven monoclonal antibodies against plasma membrane of T. vaginalis HY-1 were prepared and were investigated the effector function of them. Seven monoclonal antibodies were produced and their isotypes were Ig G1 (Tv1, Tv7), Ig G2a (Tv4), Ig M(Tv2, Tv3, Tv5, Tv6). All of the seven monoclonal antibodies recognized the determinants located on the surface of live organism by IFAT. Although these surface reactive seven monoclonal antibodies agglutinated T. vaginalis trophozoites, especially MAb Tv1 and Tv4 could agglutinate eight isolates of T. vaginalis. In immunoblot assay, MAb Tv1 & Tv4 detected single peptide with molecular weight of 60 kDa, and Tv7 detected 80 kDa. On the other hand Tv2 and Tv6 detected two (40 kDa & 120 kDa) or more peptides (62 kDa, 64 kDa & 120 kDa), and Tv3 and Tv5 did not bind any pepetide. From these results, MAb Tv1 reacted with 60 kDa, major surface membrane protein of T. vaginalis and agglutinated trichomonads.

외과적 보조의 급속상악확장술 : 치험 5예 REPORT OF 5 CASES

박충열,이용욱,송종운,김영운,박홍주,오희균,유선열,이기현,황현식 대한악안면성형재건외과학회 2002 Maxillofacial Plastic Reconstructive Surgery Vol.24 No.1

Five adults (3 cases of bilateral posterior crossbite, 2 cases of unilateral posterior crossbite) with intermaxillary discrepancy of over 5㎜ due to maxillary transverse deficiency were treated by surgically assisted rapid maxillary expansion (SARME). Bilateral maxillary horizontal osteotomies, osteotomies of pterygomaxillary junction and anterior lateral nasal wall, ans anterior palatal osteotomy were performed in 4 cases, and unilateral osteotomies in 1case, followed by palatal expansion with tooth-borne orthopedic applicance. No significant complication was observed. The mean palatal expansion was 7.2㎜ (5.8∼10.0㎜) in the maxillary first molar region. During the mean follow-up period of 4 years (5 months∼8 years 7 months), no recurrence of crossbite was observed in all cases. The SARME seemed to be a simple, stable, and reliable procedure for achieving a permanent increase of over 5㎜ in skeletal maxillary transverse width of adults.

동물과 사람유래 Vancomycin 내성 장구균의 항균제 감수성 비교

조윤상,이희수,김종만,류판동,박용호,유한상,이문한 한국수의공중보건학회 2003 예방수의학회지 Vol.27 No.1

It has been recently reported the possibility in the bansfer of antimicrobial resistance to other animals and humans. In particular, vancomycin resistant enterococci (VRE), which have been known as a principal antimicrobial resistant bacteria in humans, have been increased as a pathogen of nosocomial infections. And then animal VRE were suspected as an origin of human VRE. In this study, we isolated Enterococcus spp. from animals, identified by bio- chemical tests, examined for antimicrobial susceptibility, and then compared the antimicrobial susceptibility of VRE among each other as well as human VRE. Enterococcus iecium (29%) was predominant in Enterococcus species (n=122) isolated from animal feces in this study. E. hirae, E. iecalis, E. casseliflam and E. gallinarum were also isolated as rates of 24%, 21%, 16% and 7%, respectively. The resistance of enterococci to penicillin and tetracycline were 66% and 78%, respectively, and the susceptibility of them to chloramphenicol was 66%. Antimicrobial susceptibility test has shown that 91% of VRE from humans (n=11) was susceptible to chloramphenicol and all resistant to penicillin, rifampin and streptomycin. Seventy-five percentage of VRE from chickens (n = 12) was susceptible to rifampin and resistances of them to penicillin, tetracycline and sbeptomycin were 75%, 83%, and l00%, respectively. Therefore, we confirmed the difference of antimicrobial susceptibility from animals and humans, and the antimicrobial susceptibility test could be one of the simple and useful methods for the epidemiological survey of VRE.

Osteotome Sinus Floor Elevation Procedure를 이용한 임프란트 매식

송종운,조용기,박홍주,김영운,오희균,유선열 대한악안면성형재건외과학회 2002 Maxillofacial Plastic Reconstructive Surgery Vol.24 No.2

Maxillary sinus has an anatomic limitation to bone support available for dental implant. When there is less than 10㎜ of bone remaining between the upper alveolar ridge crest and the maxillary sinus floor, bone augmentation should be considered. In 1994, Summers reported two sinus floor elevation techniques of limmediate implant insertion, using osteotomes, for patients who have at least 5㎜ of bone remaining between the alveolar ridge crest and the maxillary sinus floor. One technique is osteotome sinus floor elevation (OSFE) procedure in which uses osteotomes to elevate the sinus floor. The other is bone-added OSFE (BAOSFE) procedure in which bone graft is added into osteotomy site. Both procedures conserve all of the remaining bone and selectively displace it upward, thereby raising sinus floor. This study was aimed to evaluate the long-term success rate of implants which had been placed by osteotome sinus floor elevation procedure. Between February 1996 and June 1998, 10 implants were placed using OSFE or BAOSFE procedures in 6 patients who had at least 6㎜ of bone remaining between the alveolar ridge crest and the sinus floor. OSFE procedures were performed in patiens, 2∼4㎜ sinus floor elevation was needed, whereas BAOSFE procedures were done in patines, more than 4㎜ sinus floor elevation was needed. During the mean follow-up period of 4 years 4 months (3 years 6 months ∼ 5 years 9 months), no implant was failed and all patients showed good functional results. OSFE and BAOSFE procedures seemed technically easier and less invasive compared to conventional sinus lift procedure. And both procedure have good success rate.