Two New Diphenylethylenes from Arundina graminifolia and Their Cytotoxicity

Yin-Ke Li,Bin Zhou,Yan-Qing Ye,Gang Du,De-Yun Niu,Chun-Yang Meng,Xue-Mei Gao,Qiu-Fen Hu 대한화학회 2013 Bulletin of the Korean Chemical Society Vol.34 No.11

Two new diphenylethylenes, gramniphenols H and I (1 and 2), together with six known diphenylethylenes (3- 8), were isolated from Arundina graminifolia. The structures of 1-8 were elucidated by spectroscopic methods including extensive 1D- and 2D-NMR techniques. Compounds 1 and 2 were evaluated for their cytotoxicity against five human tumor cell lines. Compound 1 showed cytotoxicity against PC3 cells with IC50 value of 3.5 μM. Compound 2 showed cytotoxicity against NB4 and PC3 cells with IC50 values of 3.6 and 3.8 μM, respectively.

Recombinant-attenuated Salmonella Pullorum strain expressing the hemagglutinin-neuraminidase protein of Newcastle disease virus (NDV) protects chickens against NDV and Salmonella Pullorum challenge

Ke Ding,Ke Shang,Zu-Hua Yu,Chuan Yu,Yan-Yan Jia,Lei He,Cheng-Shui Liao,Jing Li,Chun-Jie Zhang,Yin-Ju Li,Ting-Cai Wu,Xiang-chao Cheng 대한수의학회 2018 Journal of Veterinary Science Vol.19 No.2

Newcastle disease virus (NDV) and Salmonella Pullorum have significant damaging effects on the poultry industry, but no previous vaccinecan protect poultry effectively. In this study, a recombinant-attenuated S. Pullorum strain secreting the NDV hemagglutinin-neuraminidase(HN) protein, C79-13ΔcrpΔasd (pYA-HN), was constructed by using the suicide plasmid pREasd-mediated bacteria homologousrecombination method to form a new bivalent vaccine candidate against Newcastle disease (ND) and S. Pullorum disease (PD). The effectof this vaccine candidate was compared with those of the NDV LaSota and C79-13ΔcrpΔasd (pYA) strains. The serum hemagglutinationinhibition antibody titers, serum immunoglobulin G (IgG) antibodies, secretory IgA, and stimulation index in lymphocyte proliferation wereincreased significantly more (p < 0.01) in chickens inoculated with C79-13ΔcrpΔasd (pYA-HN) than with C79-13ΔcrpΔasd (pYA) but werenot significantly increased compared with the chickens immunized with the LaSota live vaccine (p > 0.05). Moreover, the novel strain provides60% and 80% protective efficacy against the NDV virulent strain F48E9 and the S. Pullorum virulent strain C79-13. In summary, in this study,a recombinant-attenuated S. Pullorum strain secreting NDV HN protein was constructed. The generation of the S. Pullorum C79-13ΔcrpΔasd(pYA-HN) strain provides a foundation for the development of an effective living-vector double vaccine against ND and PD.

Two New Diphenylethylenes from Arundina graminifolia and Their Cytotoxicity

Li, Yin-Ke,Zhou, Bin,Ye, Yan-Qing,Du, Gang,Niu, De-Yun,Meng, Chun-Yang,Gao, Xue-Mei,Hu, Qiu-Fen Korean Chemical Society 2013 Bulletin of the Korean Chemical Society Vol.34 No.11

Two new diphenylethylenes, gramniphenols H and I (1 and 2), together with six known diphenylethylenes (3-8), were isolated from Arundina graminifolia. The structures of 1-8 were elucidated by spectroscopic methods including extensive 1D- and 2D-NMR techniques. Compounds 1 and 2 were evaluated for their cytotoxicity against five human tumor cell lines. Compound 1 showed cytotoxicity against PC3 cells with $IC_{50}$ value of 3.5 ${\mu}M$. Compound 2 showed cytotoxicity against NB4 and PC3 cells with $IC_{50}$ values of 3.6 and 3.8 ${\mu}M$, respectively.

Self-Organization of Amphiphilic Diblock Rod-Coil Molecule into Supramolecular Honeycomb and Cylindrical Aggregates and Its Application as Suzuki Coupling Reaction

Ke-li Zhong,Chunchang Yang,Bingzhu Yin,Long Yi Jin,Zhegang Huang,이은지 한국고분자학회 2010 Macromolecular Research Vol.18 No.3

An aromatic amphiphilic molecule consisting of three biphenyl groups and one styrene unit linked together with ether bonds as a rigid rod segment, and poly(ethylene oxide) with 17 repeating units as a coil segment was synthesized and its self-assembly behavior in the bulk state and aqueous solution was examined by DSC, X-ray scattering, DLS and TEM. This molecule based on a rod building block self-assembles into lamellar or hexagonal perforated layer (HPL) structures in the crystalline phase or liquid crystalline mesophase. In an aqueous medium,the molecule self-assembles into cylindrical aggregates and can be used as a supramolecular reactor for the Suzuki coupling reaction.

Rheological analysis of titanium dioxide nano-whisker based electrorheological fluids

Ke Zhang,Chun Yan Gao,Hyoung Jin Choi,Jianbo Yin,Xiaopeng Zhao 한국공업화학회 2020 Journal of Industrial and Engineering Chemistry Vol.83 No.-

Understanding yield stress of electrorheological (ER)fluids becomes important for not only developmentof advanced ER materials but also their engineering applications. This study reanalyzed dynamic yieldstresses of both titanium dioxide nano-whisker- and titanium dioxide nano-particle-based ERsuspensions under an input electricalfield, based on the universal yield stress function proposed. The experimental data was plotted well to a single line by the scaled universal yield stress function via adeduced critical electricalfield value which divides electricfield dependent yield stress region with aslope of 1.5 from that with 2.0 of polarization mechanism.

An Ordered Network Polymer of Bicontinuous Cubic Structure Resulting from Photo-Polymerization of a Coil-Rod-Coil Molecule Self-Assembly

Ke Li Zhong,Tie Chen,Bing Zhu Yin,Long Yi Jin 한국고분자학회 2009 Macromolecular Research Vol.17 No.4

Fatigue behavior of concrete beams reinforced with HRBF500 steel bars

Ke Li,Xin-Ling Wang,Shuang-Yin Cao,Qing-Ping Chen 국제구조공학회 2015 Structural Engineering and Mechanics, An Int'l Jou Vol.53 No.2

The purpose of this study was to investigate experimentally the fatigue performance of reinforced concrete (RC) beams with hot-rolled ribbed fine-grained steel bars of yielding strength 500MPa (HRBF500). Three rectangular and three T-section RC beams with HRBF500 bars were constructed and tested under static and constant-amplitude cyclic loading. Prior to the application of repeated loading, all beams were initially cracked under static loading. The major test variables were the steel ratio, crosssectionalshape and stress range. The stress evolution of HRBF500 bars, the information about crack growth and the deflection developments of test beams were presented and analyzed. Rapid increases in deflections and tension steel stress occured in the early stages of fatigue loading, and were followed by a relatively stable period. Test results indicate that, the concrete beams reinforced with appropriate amount of HRBF500 bars can survive 2.5 million cycles of constant-amplitude cyclic loading with no apparent signs of damage, on condition that the initial extreme tensile stress in HRBF500 steel bars was controlled less than 150 MPa. It was also found that, the initial extreme tension steel stress, stress range, and steel ratio were the main factors that affected the fatigue properties of RC beams with HRBF500 bars, whose effects on fatigueproperties were fully discussed in this paper, while the cross-sectional shape had no significant influence in fatigue properties. The results provide important guidance for the fatigue design of concrete beams reinforced with HRBF500 steel bars.

Design and torque calculation of permanent magnet eddy current coupling with sinusoidal torque output

Ke Chen,Hongyang Shi,Yajun Guo,Lei Yin,Ziwei Bao,Hongmei Zheng 전력전자학회 2023 JOURNAL OF POWER ELECTRONICS Vol.23 No.4

Due to its unique characteristic of non-contact torque transmission between its permanent magnetic rotor and conductorrotor, permanent magnet eddy current coupling (PMECC) is preferable in transmitting torque from the outside to the insideof a sealed container, which can effectively avoid the tricky problem of dynamic seals. To mix non-uniform liquid solutionsor gas mixtures in sealed containers more effectively, a sinusoidal torque is also required. Thus, permanent magnet eddy current coupling with a slotted conductor is proposed in this paper, which can adaptively output sinusoidal torque under a real-time load when the input rotational speed is constant. Based on the equivalent magnetic circuit method, an analytical model for calculating torque is proposed. When establishing the equivalent magnetic circuit model of the PMECC, the radial magnetic flux leakage circuit is added to the equivalent magnetic circuit diagram, and the cross-sectional shape of the eddy current region is equivalent to an octagon, which simplifies the torque calculation process. The analytical model is verified by finite element method (FEM) simulation and experiments. According to a comparison, the peak and lowest values of output torque obtained by the experiments, the analytical model, and the FEM simulation are consistent. The output torque waveform obtained by the analytical model and FEM simulation are also consistent.

Synthesis and self-assembly of rod-coil molecules with n-shaped rod building block

Zhong, Ke-Li,Huang, Zhegang,Man, Zhijin,Jin, Long Yi,Yin, Bingzhu,Lee, Myongsoo Wiley Subscription Services, Inc., A Wiley Company 2010 Journal of polymer science Part A, Polymer chemist Vol.48 No.6

<P>The rod-coil molecules with n-shaped rod building block, consisting of an anthracene unit and two biphenyl groups linked together with acetylenyl bonds at the 1,8-position of anthracene as a rigid rod segment, and the alkyl or alkyloxy chains with various length (i.e., methoxy- (1), octyl- (2), hexadecyl- (3)) at the 10-position of anthracene and poly(ethylene oxide) with the number of repeating units of 7 connected with biphenyl as coil segments were synthesized. The molecular structures were characterized by <SUP>1</SUP>H NMR and MALDI-TOF mass spectroscopy. The self-assembling behavior of new type of molecules 1–3 was investigated by means of DSC, POM, and SAXS at the bulk state. These molecules with a n-shaped rod building block segment self-assemble into supramolecular structures through the combination of π–π stacking of rigid rod building blocks and microphase separation of the rod and coil blocks. SAXS studies reveal that molecules 1 and 2 show hexagonal columnar and rectangular columnar structures in the liquid crystalline phase, respectively; meanwhile, molecules 1–3 self-organize into lamellar structures in the crystalline state. In addition, self-assembling studies of molecules 1–3 by DLS and TEM indicated that these molecules self-assemble into elongated nanofibers in aqueous medium. © 2010 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 48: 1415–1422, 2010</P> <B>Graphic Abstract</B> <P>Rod-coil molecules with n-shaped rod building block were successfully synthesized. In the melt state, molecule with a methoxy group at 10 position of anthracene self-assembles into hexagonal columnar structure, Whereas molecule with an octanyl group at 10 position of anthracene self-organizes into rectangular columnar structure. Further increasing the length of alkyl chain suppresses a liquid crystalline phase and exhibits only a lamellar structure in the solid state. In aqueous medium, these molecules can self-assemble into elongated nanofiber structure with a radial aggregation of aromatic segments. <img src='wiley_img/0887624X-2010-48-6-POLA23909-gra001.gif' alt='wiley_img/0887624X-2010-48-6-POLA23909-gra001'> </P>

Fatigue behavior of concrete beams reinforced with HRBF500 steel bars

Li, Ke,Wang, Xin-Ling,Cao, Shuang-Yin,Chen, Qing-Ping Techno-Press 2015 Structural Engineering and Mechanics, An Int'l Jou Vol.53 No.2

The purpose of this study was to investigate experimentally the fatigue performance of reinforced concrete (RC) beams with hot-rolled ribbed fine-grained steel bars of yielding strength 500MPa (HRBF500). Three rectangular and three T-section RC beams with HRBF500 bars were constructed and tested under static and constant-amplitude cyclic loading. Prior to the application of repeated loading, all beams were initially cracked under static loading. The major test variables were the steel ratio, cross-sectional shape and stress range. The stress evolution of HRBF500 bars, the information about crack growth and the deflection developments of test beams were presented and analyzed. Rapid increases in deflections and tension steel stress occured in the early stages of fatigue loading, and were followed by a relatively stable period. Test results indicate that, the concrete beams reinforced with appropriate amount of HRBF500 bars can survive 2.5 million cycles of constant-amplitude cyclic loading with no apparent signs of damage, on condition that the initial extreme tensile stress in HRBF500 steel bars was controlled less than 150 MPa. It was also found that, the initial extreme tension steel stress, stress range, and steel ratio were the main factors that affected the fatigue properties of RC beams with HRBF500 bars, whose effects on fatigue properties were fully discussed in this paper, while the cross-sectional shape had no significant influence in fatigue properties. The results provide important guidance for the fatigue design of concrete beams reinforced with HRBF500 steel bars.