MiR-218-5p Suppresses the Killing Effect of Natural Killer Cell to Lung Adenocarcinoma by Targeting SHMT1

Quanjun Yang,Jingjing Li,Yili Hu,Xiaofei Tang,Lili Yu,Lihua Dong,Diandian Chen 연세대학교의과대학 2019 Yonsei medical journal Vol.60 No.6

Purpose: Lung adenocarcinoma (LA) is one of the major types of lung cancer. MicroRNAs (miRNAs) play an essential role in regulatingresponses of natural killer (NK) cells to cancer malignancy. However, the mechanism of miR-218-5p involved in the killingeffect of NK cells to LA cells remains poorly understood. Materials and Methods: The expression of miR-218-5p was examined by quantitative real-time polymerase chain reaction (qRTPCR). Serine hydroxymethyl transferase 1 (SHMT1) level was detected by qRT-PCR or western blots. Cytokines production ofinterferon-γ (IFN-γ) and tumor necrosis factor-α (TNF-α) were detected by ELISA. The killing effect of NK cells to LA cells was investigatedusing lactate dehydrogenase cytotoxicity assay kit. The interaction of miR-218-5p and SHMT1 was probed by luciferaseactivity assay. Xenograft model was established to investigate the killing effect of NK cells in vivo. Results: miR-218-5p was enhanced and SHMT1 was inhibited in NK cells of LA patients, whereas stimulation of interleukin-2(IL-2) reversed their abundances. Addition of miR-218-5p reduced IL-2-induced cytokines expression and cytotoxicity in NK-92against LA cells. Moreover, SHMT1 was negatively regulated by miR-218-5p and attenuated miR-218-5p-mediated effect on cytotoxicity,IFN-γ and TNF-α secretion in IL-2-activated NK cells. In addition, miR-218-5p exhaustion inhibited tumor growth bypromoting killing effect of NK cells. Conclusion: miR-218-5p suppresses the killing effect of NK cells to LA cells by targeting SHMT1, providing a potential target forLA treatment by ameliorating NK cells function.

Oncogenic E3 ubiquitin ligase NEDD4 binds to KLF8 and regulates the microRNA-132/NRF2 axis in bladder cancer

Mao Minghuan,Yang Liang,Hu Jingyao,Liu Bing,Zhang Xiling,Liu Yili,Wang Ping,Li Hangyu 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

The neuronally expressed developmentally downregulated 4 (NEDD4) gene encodes a ubiquitin ligase that targets the epithelial sodium channel for degradation and has been implicated in tumor growth in various cancers. Hence, in this study, we intended to characterize the functional relevance of the NEDD4-mediated Kruppel-like factor 8/microRNA-132/nuclear factor E2-related factor 2 (KLF8/miR-132/NRF2) axis in the development of bladder cancer. NEDD4 and KLF8 were overexpressed in bladder cancer tissues and were associated with poorer patient survival rates. In bladder cancer cells, NEDD4 intensified the stability and transcriptional activity of KLF8 through ubiquitination to augment cell viability and migratory ability. Our investigations revealed that NEDD4 promotes the binding of KLF8 to the miR-132 promoter region and inhibits the expression of miR-132. KLF8 inhibited the expression of miR-132 to augment the viability and migratory ability of bladder cancer cells. Furthermore, miR-132 downregulated the expression of NRF2 to restrict the viability and migratory ability of bladder cancer cells. In addition, in vivo findings verified that NEDD4 regulates the KLF8/miR-132/NRF2 axis by accelerating tumor growth and lung metastasis. In conclusion, this study highlights NEDD4 as a potential therapeutic target against tumor recurrence and metastasis in bladder cancer.

Surface Display of the HPV L1 Capsid Protein by the Autotransporter Shigella IcsA

Dan Xu,Xiaofeng Yang,Depu Wang,Jun Yu,Yili Wang 한국미생물학회 2014 The journal of microbiology Vol.52 No.1

Autotransporters have become attractive tools for surface expression of foreign proteins in Gram-negative bacteria. In this study, the Shigella autotransporter IcsA, has been exploited to express the human papillomavirus (HPV) type 16 L1 capsid protein in Shigella sonnei and Escherichia coli. The L1 gene was fused in-frame to replace the coding sequence of the IcsA passenger domain that is responsible for actin-based motility. The resultant hybrid protein could be detected by an anti-L1 antibody on the surface of S. sonnei and E. coli. In E. coli, the protein was expressed on the entire surface of the bacterium. In contrast, the protein was detected mainly at one pole of the Shigella bacterium. However, the protein became evenly distributed on the surface of the Shigella bacterium when the icsP gene was removed. Our study demonstrated the possibility of exploiting autotransporters for surface expression of large, heterologous viral proteins,which may be a useful strategy for vaccine development.

Numerical investigation of the mixing process in a Twin Cam Mixer: Influence of triangular cam height-base ratio and eccentricity

Yu He,Xiwen Li,Jiecai Long,Baojun Shen,Zhibin Sun,Yili Yang,Xiaobin Zhan 한국화학공학회 2021 Korean Journal of Chemical Engineering Vol.38 No.3

The twin cam mixer (TCM), as a general-purpose mixer, shares many attributes in common with 3D industrial mixers, like the internal mixer. We investigated the mixing process in a 2D TCM with two identical isosceles triangular cams rotating at 0.5 rpm. A 2D numerical model coupled with the species transport model was employed to study the influence of cam height-base ratio and eccentricity qualitatively and quantitatively, and both were found to have a significant effect on the mixing behavior of the mixer. Furthermore, a dimensionless parameter, named the modified pressurization coefficient, is put forward to quantify the geometry of the mixer. The logarithmic relationship between the modified pressurization coefficient and the mixing quality was discovered and expected to provide new ideas for establishing the relationship between the geometric parameters of a mixer and its mixing performance.

Computational fluid dynamic analysis of mass transfer and hydrodynamics in a planetary centrifugal bioreactor

Baojun Shen,Xiaobin Zhan,Yu He,Zhibin Sun,Jiecai Long,Yili Yang,Xiwen Li 한국화학공학회 2021 Korean Journal of Chemical Engineering Vol.38 No.7

Planetary centrifugal bioreactors are promising candidates for cell culture platforms since there is no pollution caused by stirring blades. In this work, the fluid structure in a planetary centrifugal bioreactor was investigated using the computational fluid dynamics (CFD) method. The effects of operating conditions on the oxygen transfer rate (OTR), mixing efficiency and shear environment of the bioreactor were studied with the revolution speed (N) ranging from 60 to 160 rpm and the rotation-to-revolution speed ratio (i) from 2 to 1. The results show that the volumetric mass transfer coefficient (kLa), turbulence intensity, volumetric power consumption, and shear stress increase along with the increase of the revolution and rotation speeds. Furthermore, the rotation in the opposite direction to the revolution is beneficial to the performance of the bioreactor. The planetary centrifugal bioreactor has a higher kLa of 50- 200/h and a lower average shear stress of 0.01-0.05 Pa in comparison with conventional stirred tank bioreactors, which makes it suitable for biological culture of oxygen-consuming cells and shear-sensitive cells.

Effect of Physical Activity on the Association Between Dietary Fiber and Constipation: Evidence From the National Health and Nutrition Examination Survey 2005-2010

( Yi Li ),( Wei-dong Tong ),( Yang Qian ) 대한소화기기능성질환·운동학회(구 대한소화관운동학회) 2021 Journal of Neurogastroenterology and Motility (JNM Vol.27 No.1

Background/Aims The effect of physical activity on the relationship between dietary fiber intake and constipation has not been comprehensively studied. This study aims to explore the impact of physical activity. Methods Data were obtained from 3 cycles of the National Health and Nutrition Examination Survey (NHANES) 2005-2010 and included a total of 13 941 participants aged ≥ 20 years. Multiple logistic regression analysis was used to investigate the independent association between dietary fiber and constipation. Interaction analysis was also performed to analyze the relationship between dietary fiber and constipation in different physical activity groups. Results Among non-active participants, dietary fiber intake did not associate with stool consistency (OR, 1.02; 95% CI, 0.98-1.05; P = 0.407). For physically active participants, 1-gram unit increase in dietary fiber intake reduced the risk of stool consistency by 3% (OR, 0.97; 95% CI, 0.94-0.99; P = 0.020). Moreover, the relationship between dietary fiber intake and stool consistency was significantly different for groups with different levels of physical activity (P interaction = 0.044). However, dietary fiber intake was not related to stool frequency among non-active participants (OR, 0.99; 95% CI, 0.94-1.05; P = 0.767) nor physically active participants (OR, 1.01; 95% CI, 0.97-1.04; P = 0.751). Conclusions Increasing dietary fiber intake was associated with stool consistency-related constipation among physically active participants, but not among non-active participants. However, increasing dietary fiber intake is not significantly associated with stool frequency in different physical activity groups. (J Neurogastroenterol Motil 2021;27:97-107)

Numerical Simulation of the Aerodynamic Influence of Aircrafts During Aerial Refueling with Engine Jet

Yi Li,Yang Zhang,Junqiang Bai 한국항공우주학회 2020 International Journal of Aeronautical and Space Sc Vol.21 No.1

Aerial refueling technology has been widely applied in various fields and it is one of the hotspots but difficulties for the aeronautical technologies. DLR-F6 WBNP model is used as a tanker and a fighter model is used as a receiver. The flow field of Probe–Drogue refueling and Flying Boom refueling is numerically simulated using the Reynolds-averaged Navier–Stokes equations, and the effects of the jet flow and the aerodynamic characteristics of the receiver are taken into consideration. The results indicate that the effect of downwash of the tanker reduces the lift coefficient and decreases the pitching moment coefficient of the receiver. The jet flow of tanker increases the dynamic pressure while decreases the local angle of attack, which increases the pressure difference between the upper and lower surfaces of receiver. Compared with the results without jet, the jet flow can increase the lift and the drag of the receiver and reduces the pitching moment, and even cause the change of rolling moment direction. Therefore, engine jet is an important factor when simulating aerial refueling.