항산화제 섭취가 탈진적 운동 시 장딴지근의 GPx 및 GR의 활성에 미치는 영향

정성태,김연수,이왕록,박익렬,강현주 서울대학교 체육연구소 2001 서울大學校 體育硏究所論集 Vol.22 No.1

The purpose of this present study was to investigate the effect of maximal exercise and a long-term antioxidants supplementation on antioxidant enzyme(GPx and GR) activity of gastrocnemius muscle in rats. Twenty four male Sprague-Dawley rats(4 weeks old) were used as subjects. The subjects were divided into 4 groups such as, CON(n=6 in control group), NAE(n=6 in non antioxidants exercise group), AC(n=6 in antioxidants control group) or AE(n=6 in antioxidants exercise group). Non-antioxidants group(CON & NAE) fed general food(Samyang, Inc., Korea) for 16 weeks. Antioxidants group(AC & AE) fed the general food and the mixed antioxidants which were consist of vitamin E(200 lu/kg/day), vitamin C(50 mg/rat/day), vitamin B6(250 ug/kg/day), β carotene(300 mg/kg/day) and selenomethionine(0.1 mg/mg/day), The weight of each subject group was weighed 2-3 times per week. After the diet experiment for 16 weeks, exercise groups(NAE & AE) completed acute treadmill running(speed increased gradually to 25 m/min, 15% uphill grade, ∼50±10 min) until exhaustion. Immediately after exhaustive treadmill exercise, all the subjects were sacrificed for the isolation of gastrocnemius. Then, GPx(Glutathione Peroxidase) and GR(Glutathione Reductase) of the gastrocnemlus muscle were assayed. The protein concentration in the muscle and heart was determined by the Lowry method. After finishing the analysis of antioxidant activity, statistical analysis was carried out by two-way ANOVA and independant t-test. The results of this study can be summarized as follows: Maximal exercise increased the GPx and GR activity of gastrocnemius muscle, Further, a long-term antioxidants supplementation more significantly increased the GPx and GR activity of gastrocnemius muscle than those of non-antioxidants group. In conclusion, the present results clearly show that the exhaustive maximal exercise gave rise to increase oxidative stress which led to oxidative damage so that the antioxidant enzyme activity of gastrocnemius muscle were increased. Further, it has been found that the long-term antioxidants supplementation could decrease oxidative stress in the tissues so that the antioxidant enzyme activity of skeletal muscles was more increased.

기초군사훈련이 체력과 신체구성에 미치는 영향

정영수,우재홍,박익렬,박성태,김연수 서울대학교 체육연구소 2000 서울大學校 體育硏究所論集 Vol.21 No.2

The purpose of this study was to investigate the effects of basic military training on the physical fitness and body composition of midshipmen. The test on the twenty subjects were done twice at the beginning and end of their 5-week's basic training with same variables and order in both tests in Naval Academy, Jin-Hae. The source variables of physical fitness in the test were muscle strength, muscle endurance, muscle power, agility, balance, flexibility and so on. The source variable of body composition were body weight, fat-free mass, percentage of body fat and so on. The sum of abdomen, triceps, and suprailium skinfold thickness were measured in order to assess alterations in fat-free mass and percentage of body fat. Paired sample t-test was used for data analysis and the significance level was set at the 0.05. The results of this study were as follow: 1.muscle endurance, balance, flexibility, endurance time(p〈0.01) and muscle power(p〈0.05) were increased after training programme. 2.muscle strength and agility showed tendency to increase. 3.Body weight and free-fat mass were increased but % body fat were decreased after training programme(p〈0.01). It might be concluded from the test that basic military training in Korean Naval Academy is effective for the increase physical fitness, cardiopulmonary function, fat-free mass and for the reduce of body fat percentage.

만성정신질환자의 계속입원치료 심사에 관한 연구

정인원,박환규,정연복,김수일,원구연,김교형,신철진 大韓神經精神醫學會 1999 신경정신의학 Vol.38 No.6

연구목적: 정신보건법에 근거하여 매월 정기적으로 개최된 충청북도 정신보건심판위원회를 통하여 정신질환자의 계속입원치료 청구 심사의 현황과 문제점을 파악하고 개선사항을 지적함으로써 정신질환자의 효율적인 관리를 유도하고 인권남용의 여지를 차단하고자 한다. 방 법: 충청북도 내의 정신의료기관에서 계속입원치료 심사청구서 및 진단서, 보호의무자의 동의서 등을 제출한 정신질환자들을 대상으로 계속입원치료 청구에 대한 승인 여부를 매달 정기적으로 심사하였다. 1년 6개월여동안 심사한 자료를 정리하여 진단 및 보호의무자, 의료보장별 특성과 불승인률 등을 분석하였다. 결 과: 충청북도 심판위원회에 계속입원치료를 청구한 정신질환자는 21차례의 위원회 동안 총 7,981명이었으며, 진단별로 정신분열병은 80.9%, 알콜중독은 8.1%, 치매 등 기타는 11.0%이었다. 또한 보호의무자는 시장 또는 군수가 29.8%, 부모가 26.9%, 형제가 26.1%, 부부가 6.3%, 자녀가 5.9%, 기타가 5.0%로 나타났으며, 의료보장은 의료보호가 73.0%, 의료보험이 27.0%이었다. 이들 중에서 196명이 계속입원치료를 불승인받아서 전체 불승인률은 2.46%이었으며 정신분열병과 알콜중독 환자의 진단별 불승인률은 각각 0.73%와 17.6%이었다. 결 론: 정신과 병상의 대부분이 장기적으로 입원하는 만성 정신질환자들로 구성되어 있어서 이들에 대한 별도의 대책이 시급하다. 또한 계속입원치료가 불승인되어 퇴원한 환자들의 재활 및 사회적응을 위한 사회적 지지 체계가 부족하며, 특히 환자의 인권보호를 위한 구체적인 기준 등이 필요하다. Objectives: This study was to develop effective managements and to avoid the abuse of human rights in mentally-ill patients. The Mental Health Judgement Board of Chungchongbuk-do province has been held monthly since August, 1997 according to the Mental Health Act. In this study, the procedures and the problems of judging continuing hospitalization of chronic mentally-ill patients were reviewed. Methods: The mentally-ill patients who submitted the request for continuing hospitalization with the certificate of charged doctor and the agreement of caregiver were reviewed by the Mental Health Judgement Board of Chungchongbuk-do province. The analysis of the diagnoses, caregivers, medical care systems, and rejection rate for the patients were done. Results: Total mumber of mentally-ill patients who requested for continuing hospitalization were 7,981 from twenty-one monthly meetings. The diagnostic distributions were 80.9% for schizophrenic patients, 8.1% for alcoholics and 11.0% for others including dementia. As for the caretakers, the rate of majors was 29.8%, parents 26.9%, sibling 26.1%, spouse 6.3%, offspring 5.9%, and others 5.0%. And 73.0% of the patients were on Medicaid and 27.0% were insured. The patients who got the rejection for continuing hospitalization were 196 at the rate of 2.46%. And the rejection rates of schIzophrenic and alcoholic patients were 0.73% and 17.6%, respectively. Conclusions: New policies for decreasing longterm hospitalization of chronic mentally-ill patients are required. And the social support systems for psychiatric rehabilitation and readjustment are presently insufficient for the already discharged mentally-ill. Moreover, the practical guidelines for human rights of patients remains to be suggested.

인체 자궁암 세포에서 천연 성분이 P-당단백질의 활성에 미치는 영향

정수연,고은정,김나형,성민경,장정옥,이화정 이화여자대학교 약학연구소 2005 藥學硏究論文集 Vol.- No.15

Multidrug resistance (MDR) of cancer cells is, at least in part, associated with the overexpression of P-gly-coprotein (P-gp). Many studies hve demonstrated that natural compounds obtained from fruits, vegetables, teas and medicinal plants may modulate P-gp activity. The objective of the present investigation was to examine the effect of seven natural compounds on the P-gp activity in human uterine sarcoma cell line, MES-SA/DX5. Daunomycin uptake was significantly increased by biochanin A and silymarin (p<0.0001) whereas it was reduced by morin (p<0.01). The efflux of daunomycin from the cells was significantly inhibited by biochanin A, morin, cephalotaxine, berberine (p<0.05) and silymarin (p<0.0001). Biochanin A, berberine and silymarin significantly decreased IC_(50) value of daunomycin (p<0.05) while morin increased it (p<0.05). These results suggest that some natural compounds such as biochanin A and silymarin may inhibit P-gp funciton and can by developed as MDR reversing agents to improve the efficacy of chemotherapeutic drugs when administered concomitantly.

진화 알고리듬을 위한 객체지향 모델링과 클래스 라이브러리 구현

정호연,이수연,곽재승,김용주,박기태,현철주 한국경영과학회 2000 經營 科學 Vol.17 No.2

In evolutionary algorithm, there exist various models for the evolution of the population with respect to schemes and strategies for reproduction, in the application of the algorithm to a specific problem, one model suitable to the problem is to be properly chosen and a program expert or a software is needed to help implement and test a designed algorithm. In this study, object oriented modeling and the class library for simple evolutionary algorithms(SEA) with one population is developed. The library proposed here can be used as a generalized tool for solving problems in a wide range of domains.

美國急進主義運動의 擡頭와 衰退要因硏究

鄭然植,金慶麟,陳壽美,金東根 慶北大學校 1986 論文集 Vol.42 No.-

The purpose of this study is to explore the unfolding of the radical movement in the United States during the turn of this century. It particularly concerns about the factors determining the uprising of the radicalism at the end of the 19th century and its fall with the World War Ⅰ. The rise of American radical movement can be traced back to the industrializing era after the Civil War. It started as a reaction to the evils resulted from the rapid industrialization. Initially several movements broke out sporadically, however, until the radical ideology from Europe was flowed in. Then, the radical movement was accelerated. During this period, the leading figures who particularly influenced the development of the movement were: De Leon from the Socialist Labor Party; Gompers from the American Federation of Labor; Debs from the Socialist Party; and Haywood from the Industrial Workers of the World, etc. The movement in this era was unfolded the following historical phases: from 1870s to 1880s, the preparation stage of organizing the movement; 1890s, early action stage characterized by the Populist Movement, Pullman Strike, etc.; 1900s, the flourishing phase led by the Socialist Party and IWW; the declining phase was followed since the end of 1910s; after 1920s, American pragmatism again prevailed over radicalism. Factors attributed to the rise of the radical movement are: depravation of entrepreneurship; recurrent economic crises; deterioration of labor conditions; the influence of Marxism; and insensitive attitudes on the part of the capitalists and government. This paper also suggests the factors determining the decline of the movement: the rise of the American patriotism around the World War Ⅰ era; government's strong counteractions; anti-socialism prevailed by the negative impact of the Soviet Revolution; collapse of the movement organizations; economic recovery after the War; and the government's implementations of reforming policies. At conclusion, radicalism in the United States has played the preventive role against the risk of irreversible corruption of the Establishment and its rigidity.

일정축력 및 반복 횡하중을 받는 철골철근콘크리트 기둥의 내력과 변형성능

정수영,연길환,임경택,윤석천,한병찬 忠南大學校 産業技術硏究所 1995 산업기술연구논문집 Vol.10 No.2

An experimental investigation was conducted to investigate the strength and deformation capacity of Wide Flange Steel Encased Reinforced Concrete(SRC) Columns subjected to constant axial compression and cyclic lateral loading conditions. Six one-third scale specimens were tested, each consisting of a structural steel size encased in reinforced concrete. The parameters studied in the test program included the effects of B/t ratios of encased steel(=13, 18, 23), ratios of axial force(=1/3, 1/6). As a result, capacity prediction based on superimposing the moment-axial-load interaction surfaces of the steel shape and reinforced concrete produced good results; the average of the experimental-to-predicted capacity ratio being equal to 0.99∼1.20. The observed hysteretic behavior of the test specimens indicated that adequated ductility can be achieved and the concrete core must be inhibited to buckling of wide flange.

비균질성을 고려한 김포매립장의 수리전도도 추정

장연수,정상용 동국대학교 산업기술연구원 1995 산업기술논문집 Vol.6 No.-

본 논문에서는 지구통계학적 방법을 이용하여 오염물 이동에 큰 영향을 미치는 김포 쓰레기 매립지의 수리전도도의 비균질성에 대해서 분석하였다. 수리전도도는 가압 실내투수 실험 자료와 현장 투수실험 자료를 이용하였고 지구 통계학적 방법으로는 일반 크리깅과 조건부 시뮬레이션 방법을 이용하였다. 그 결과 실내수리전도도의 범위는 1.2×10 exp (-7) - 9.6×10 exp (-7)cm/sec, 1.1×10 exp (-8) - 7.17×10 exp (-6)cm/sec, 그리고 현장 수리전도도는 4.27×10 exp (-9) - 2.02×10 exp (-6)cm/sec, 3.34×10 exp (-9) - 1.43×10 exp (-5)cm/sec가 일반 크리깅과 조건부 시뮬레이션 방법에서 각각 구해졌다. 대체로 현장 수리전도도는 실내 수리전도도 자료에 비하여 10에서 100배 정도 컸으며 일반 크리깅 해석보다 조건부 시뮬레이션에 의한 수리전도도의 비균질성이 크게 나타났다. In this paper, the heterogeneity of hydraulic conductivity of Kimpo Waste Landfill which has large influence on the migration of contaminant transport is analized using Geostatistical methods. The hydraulic conductivity data are obtained from laboratory pressurized permeability test and in-situ slug test. The Geostatistical methods used in this analysis are Ordinary Kriging and Conditional simulation. The results show that the range of laboratory hydraulic conductivity data was 1.2×10 exp (-7) - 9.6×10 exp (-7)cm/sec obtained from Kriging analysis and 1.1×10 exp (-8) - 7.17×10 exp (-6)cm/sec from Conditional simulation. The in-situ hydraulic conductivity data have the range of 4.27×10 exp (-9) - 2.02×10 exp (-6)cm/sec from Kriging analysis and 3.34×10 exp (-9) - 1.43×10 exp (-5)cm/sec from Conditional simulation. It can be recognized that in-situ hydraulic conductivity data have magnitude 10 to 100 times higher than the laboratory hydraulic conductivity data and the heterogeneities of hydraulic conductivity obtained from Conditional simulation are greater than those obtained from Ordinary Kriging analysis.

백서 해마에서 카이닌산에 의한 조기유전자의 발현과 p42, p44 MAPK 및 EIK-1 인산화의 발달 단계에 따른 변화

정희연,김수진,김종흔,정선주,박주배,김용식,조수철 大韓神經精神醫學會 1999 신경정신의학 Vol.38 No.4

연구목적 : 어린 백서에게 카이닌산(kainic acid, KA)을 주사하여 발작을 일으킨 후, 해마에서 조기유전자 -c-fos, junB, 및 TIS1의 발현 유도 양상을 발달 단계별로 조사하여 전기경련충격(electroconvulsive shock, ECS)에서의 결과와 비교함으로써 백서 뇌 신호전달계의 성숙과정을 파악하고자 하였다. 그리고 KA 주사후 p42, p44 mitogen activated protein kinase(MAPK)의 인산화 및 그에 의해 활성화되는 것으로 알려진 전사인자 Elk-1의 인산화를 관찰하여, KA 주사후 MAPK 신호절달계를 통한 c-fos의 발현로 알려진 전사인자 Elk-1의 인산화를 관찰하여, KA 주사후 MAPK 신호전달계를 통한 c-fos의 발현 경로를 발달 단계별로 밝히고자 하였다. 방 법 : 생후 7, 14 21일 된 수컷 백서에서 KA를 복강내 주사한 후, 백서 해마에서 조기유전자의 발현 양상은 northern blot analysis로, p42, p44 MAPK와 Elk-1의 인산화는 immunoblotting으로 관찰하였다. 결 론 : 생후 7일된 백서의 해마에서는 ECS와는 달리, KA에 의한 세 가지 조기유전자의 발현이 관찰되지 않았다. 그러나 생후 14일부터 이들 조기유전자의 뚜렷한 발현을 관찰할 수 있었고 생후 21일에는 성숙한 백서와 같은 수준의 발현 양상을 볼 수 있었다. 세 가지 유전자 모두 백서의 연령과 KA 주사 후 시간에 따른 발현 유도 양상은 비슷하였다. p42, p44 MAPK는 생후 7일부터 상당한 수준의 기저치 인산화가 관찰되었으나, KA에 의한 인산화 증가는 생후 14일부터 관찰되었다. Elk-1의 인상화 역시 생후 7일부터 높은 수준으로 관찰되었으나 KA 주사 후 시간에 따른 Elk-1 인산화의 변화는 관찰할 수 없었다. 결 론 : ECS와 KA가 조기유전자 발현 양상에 차이를 보이는 것은 이들 유전자의 발현과 관련되어 활성화되는 신호전달경로의 차이 때문으로 생각된다. 백서 해마에서 KA에 의한 MAPK 활성화에 관여하는 신호 전달 기구는 연령이 높아짐에 따라 점차 성숙하고, MAPK의 활성화로 전달된 신호는 Elk-1 이외의 다른 경로를 통해 c-fos 발현을 조절하는 것으로 추측된다. Objectives : In order to investigate the maturational process of intracellular signal transduction system in rat brain, we studied the induction of the immediate early genes(IEGs)-c-fos, iunB, and TIS1 in each developmental stage after kainic acid(KA)-induced seizure in young rat hippocampus and then compared these with the results after electroconvulsive shock(ECS) And to elucidate the induction mechanism of c-fos via mitogen-activated protein kinase(MAPK) by KA in each developmental stage, we investigated the phosphorylation of p42, p44 MAPK and Elk-1 after KA treatment in young rat hippocampus. Methods : We examined the induction patterns of IEGs by northern blot analysis, and the phosphorylation of p42, p44 MAPK and Elk-1 by immunoblotting in rat hippocampus at post-natal day 7, 14, and 21(P7, P14 & P21), respectively after intraperitoneal injection of KA. Results : Unlike ECS, KA did not induce c-fos, junB, and TIS1 in P7 hippocampus. But these genes were apparently induced at P14 and to an adult level at P21. These three IEGs showed similar temporal patterns of induction in each developmental stage. Although the basal level of phosphorylated 42p, 44p MAPK was considerable in P7 rat hippocampus, the increase or phosphorylation after KA treatment was observed at P14. While the phosphorylation of Elk-1 was detected with high basal level in P7 rat, the amount of phosphorylated Elk-1 was not changed after KA treatment. Conclusion : Our results suggest that the differences in IEGs induction patterns between KA and ECS may be due to the differences in the activated signal transduction pathways. And our results also implicate that the signal transduction system involved in MAPK phosphorylation after KA treatment mature with aging and c-fos induction via MAPK activation may be regulated through some pathways other than Elk-1 in rat hippocampus.

주의력결핍과잉행동장애와 Alpha-1C-Adrenergic 수용체 유전자와의 연관성에 대한 연구

조수철,김재원,김붕년,황준원,박미라,김순애,조대연,유희정,정운선,손정우,박태원 大韓神經精神醫學會 2008 신경정신의학 Vol.47 No.1

Objectives : Neurobiological and pharmacological research has suggested that the dysregulation of the central noradrenergic systems might be involved in the pathophysiology of ADHD. The aim of this study was to examine the association of the alpha-1C-adrenergic receptor gene (ADRA1C) with ADHD in Korean subjects. Methods : In a case-control study, we assessed 186 DSM-IV ADHD probands and 150 normal controls. One hundred and eight trios were studied in a family-based association analysis. Psychiatric diagnoses were derived through structured diagnostic interviews. For the clinical evaluation of the ADHD subjects, the Child Behavior Checklist (CBCL), the ADHD Rating Scale-IV (ARS) and the Junior Temperament and Character Inventory (JTCI) were administered. A computerized continuous performance test (CPT) was used to measure the inattention and impulsivity of the ADHD children. Results : There were no significant differences in the genotype or allele frequencies of the ADRA1C PstI polymorphism between the ADHD and control group (p>0.05). The transmission disequilibrium test (TDT) analysis observed no evidence for biased transmission of any of the alleles of the PstI polymorphism. There were no significant differences in the CPT or JTCI profiles between those ADHD subjects with the CC genotype and those with the other (CT+TT) genotypes at the PstI polymorphism. Conclusion : The results of this study do not support the ADRA1C as a major genetic susceptibility factor in ADHD.