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Capsicum chinense, 재래종 및 저항성 고추 유전자원에서 역병 저항성 계통 선발
김정훈,여승호,김동우,배수연,한정혜,황희숙,김병수 慶北大學校農業科學技術硏究所 2002 慶北大農學誌 Vol.20 No.-
2000년도에 검정에서 역병에 살아남은 개체들로부터 채종하여 육성한 Capsicum chinense 31계통에 대하여 역병 저항성을 검정한 결과 고도의 저항성을 나타내는 것은 발견되지 않았다. 2001년도의 검정에서 역병에 살아남은 개체로부터 채종한 재래종 26계통에 대하여 다시 역병 저항성 검정을 실시한 결과 KC180, KC230, KC195, KC194에서 다수의 개체가 살아남아 저항성을 나타내었다. 그러나 KC180과 KC230은 각각 AC2258과 CM334와 혼종된 것으로 관찰되었다. KC195와 KC194는 재래종의 형질을 유지하고 있는 것으로 관찰되었다. CM334의 보존 증식과정에 자연교잡이 일어난 것으로 보여 이의 순도향상을 위하여 채종년도별로 시료를 꺼내어 역병저항성 검정을 실시한 결과 가장 오래된 1992년도 채종종자에서부터 약간의 이형주가 관찰도기 시작하여 1995년부터 2001년도까지 시간이 경과함에 따라 많이 변형되어 있는 것을 알 수 있었다. 1992년도 종자에서 이형주를 제거하고 원형의 개체로부터 자식종자를 대량으로 채종하였다. 함께 공시한 AC2258은 순수한 것으로 확인되었다. 1995년도 채종 CM334 종자에서는 비록 혼종은 되었으나 측지발생이 적은 개체들이 발견되어 이들을 개체 선발하여 역병에 저항성이며 측지발생이 적은 계통으로 육성하고 있다. A total of 31 c. chinense lines selected from 2000 screening were tested again for resistance to P. capsici but resistance was not found in the lines. A total of 26 selections from Korean land races tested 2001 spring were tested again for resistance to P. capsici. KC180, KC230, KC195 and KC194 showed moderate resistance to P. capsici. However, it was apparent on the basis of hortivultural characteristics that KC180 and KC230 had been naturally crossed with AC2258 and CM334, respectively. CM334 and AC2248 seed lots that were increased in different years were taken out and tested for resistance to improve their genetic purity because the resistant genetic resources have been showing some off-types in the population. Off-types began to be found in 1992 seed lots and the proportion and degree of the offs was increasing with time up to 2001. Plants true to the type in 1992 seed lots were selected and their inbred seeds were mass produced in a net cage in the greenhouse. AC2258 included in the experiment number of lateral branching at cotyledonary axil, although they were off from the original in the experiment together was uniform. In 1995 seed lost of CM334, plants with resistance to P. capsici and low or no number of lateral branching at cotyledonary axil, although they were off from the original CM334, were found and selection was applied to breed lines fixed in the characters.
Aging process is accompanied by increase of transglutaminase C
Park, Jeong Soo,Kwak, Sahng June,Choi, Jee Young,Yeo, Eui Ju,Kim, Kyung Ok,Seong, Sang Cheol,Hwang, Yong Chul,Park, Sang Chul,Park, Yong Hoon,Han, Jeong A 한림대학교 한림과학원 부설 환경ㆍ생명과학연구소 1998 국제학술회의 Vol.1998 No.-
Crosslinking has been suggested as one of the mechanisms involved in the aging process. Among the various random or enzyme-mediated crosslinking reactions, transglutaminase (TGase)-catalyzed crosslinking activity has been proposed for its possible involvement in cell proliferation, differentiation, carcinogenesis, programmed death and aging. Moreover, recent findings of TGase C as a putative signal transducer and cell cycle regulator has renewed interest in the study of TGase C in relation to aging phenomena. The ubiquitous presence of TGase C compared to the organ-specific localization of other types of TGases has attracted special attention as a cellular aging device. In the present study, for in vitro studies we have analyzed the comparative pattern of TGase C in young and old human red blood cells, separated by density defferentiation, and in early and late-passage of hydrogen peroxide-treated human primary fibroblasts. For in vivo study, we monitored the age-dependent changes of TGase C in the liver and brain tissues of 4, 12, 18, and 24-month-old Sprague-Dawley rats. We obtained evidence that both the activity and protein levels of TGase C were high in old RBC and late-passage of hydrogen peroxide-treated vibroblasts. Similar findings were seen in liver and brain tissue such as age-dependent increases in TGase activity and protein level in an organ-specific pattern. These data suggest that TGase C might play an active role in the cellular process with age.
생화학,분자생물학 : 한국 토양 환경유래의 N-acyl amino acid synthase 유전자에 의한 대장균 내 항생제 N-Lauroyl tyrosine 생산
여윤수 ( Yun Soo Yeo ),임융호 ( Yoon Ho Lim ),김정봉 ( Jeong Bong Kim ),양정모 ( Jung Mo Yang ),이창묵 ( Chang Muk Lee ),김수진 ( Soo Jin Kim ),박민선 ( Min Seon Park ),구본성 ( Bon Sung Koo ),윤상홍 ( Sang Hong Yoon ) 한국응용생명화학회(구 한국농화학회) 2007 Applied Biological Chemistry (Appl Biol Chem) Vol.50 No.4
마이크로캡슐을 활용한 콘크리트 구조물의 균열관리 시스템
김여정(Kim, Yeo-Jeong),유도규(You, Do-Gyu),정수은(Jeong, Soo-Eun),한우성(Han, Woo-Seong),함승민(Ham, Seung-Min),박기봉(Park, Ki-Bong) 대한건축학회 2021 대한건축학회 학술발표대회 논문집 Vol.41 No.2
When microcracks on the concrete surface are affected by deterioration factors, they promote cracking, corrosion of internal rebar, and deterioration of durability, which affects durability. Therefore, maintenance of the structure is important, but it is difficult to find micro-cracks with the naked eye at present. Therefore, this study aims to develop a system capable of identifying cracks in structures by mixing microcapsules containing a fluorescent crack sensor in a polymer matrix and applying them on the surface to disperse the fluorescent crack sensor in the microcapsule when cracks occur.