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Yeji Kang,Namhee Kim,Yunhwan Lee,Xiangxue An,Yoon-Sok Chung,Yoo Kyoung Park 한국임상영양학회 2023 Clinical Nutrition Research Vol.12 No.3
Early prevention of sarcopenia can be an important strategy for muscle maintenance, but most studies target subjects at slightly pre-sarcopenic state. Our previous paper describes the effect of protein supplements rich in leucine and vitamin D on muscle condition, and in this paper, we performed a sub-analysis to evaluate who benefitted the most in terms of improvement in muscle health. A 12-week randomized clinical trial of 120 healthy adults (aged 50 to 80) assigned to an intervention group (n = 60) or control group (n = 60) were analyzed. Subjects in the intervention group received, twice per day, a protein supplement containing (per serving) 800 IU of vitamin D, 20 g of protein (3 g of total leucine), 300 mg of calcium, 1.1 g of fat, and 2.5 g of carbohydrate. The subjects were classified into ‘insufficient’ and ‘sufficient’ groups at 25-hydroxyvitamin D (25[OH]D) value of 30 ng/mL. The skeletal muscle mass index normalized to the square of the skeletal muscle mass (SMM) height (kg/ m2 ) increased significantly in the ‘insufficient group’ difference value of change between weeks 0 and 12 (Δ1.07 ± 2.20; p = 0.037). The SMM normalized by body weight (kg/kg, %) was higher, but not significantly, in the insufficient group (Δ0.38 ± 0.69; p = 0.050). For people with insufficient (serum 25[OH]D), supplemental intake of protein and vitamin D, calcium, and leucine and adequate energy intake increases muscle mass in middle-aged and older adults and would be likely to exert a beneficial effect on muscle health.
Gas bubble-assisted extraction method for efficient recovery of paclitaxel from Taxus chinensis
Kang Yeji,강다연,김진현 한국생물공학회 2025 Biotechnology and Bioprocess Engineering Vol.30 No.2
In this study, a gas bubble-assisted extraction method was developed for the efficient recovery of paclitaxel from the biomass of Taxus chinensis. The bubble size in the extraction solution was measured by varying the gas flow rate and air sparger pore size. Furthermore, the gas bubble-assisted extraction characteristics, kinetics, and mechanism according to contact time and bubble size were investigated. The average bubble size was 2.4–6.6 mm at gas flow rates of 1.5–5.5 L/min and air sparger pore sizes of 10–43 μm, and the paclitaxel extraction efficiency was significantly increased as the bubble size increased. In particular, most of the paclitaxel (~ 97%) could be recovered from the biomass in just one extraction at bubble sizes of 6.4 mm or larger. According to the intraparticle diffusion model, as the bubble size increased, the washing and diffusion steps occurred almost simultaneously, and the extraction rate constant, effective diffusion coefficient, and mass transfer coefficient increased. In addition, the external resistance for mass transfer was negligible since it had a high Biot number (> 20), and the solvent and solute were efficiently mixed.
Kang Yeji,Jin‑Hyun Kim 한국생물공학회 2025 Biotechnology and Bioprocess Engineering Vol.30 No.3
In this study, an ultrasonic desorption process was developed for the efficient recovery of paclitaxel from Taxus chinensis culture supernatants using a commercial adsorbent, Diaion HP-20. In addition, the reusability of the adsorbent was investigated. The optimal ultrasonic power, operating time, adsorbent/solvent ratio, temperature, solvent type, and concentration for ultrasonic desorption were 180 W, 20 min, 1:30 w/v, 25 °C, methanol, and 100%, respectively. Furthermore, all of the paclitaxel could be recovered after three desorption cycles. The high bubble collapse intensity of acoustic cavitation played a more decisive role than the high bubble density of hydrodynamic cavitation. Diaion HP-20 maintained a high adsorption efficiency of over 95% even after five regenerations, and the stability of the adsorbent was confirmed through electron microscopy, scanning electron microscopy, X-ray diffraction, and Fourier-transform infrared spectroscopy analyses.
노인 당뇨병 인지자의 연령에 따른 삶의 질 수준 분석: 국민건강영양조사를 이용하여
강예지(Yeji Kang),박경(Kyong Park) 한국영양학회 2020 Journal of Nutrition and Health Vol.53 No.2
본 연구는 국민건강영양조사 2008–2016년 자료를 이용하였고, 그중 자신이 당뇨병을 앓고 있다는 것을 인지하고 있는 65세 이상 노인들을 대상으로 분석을 실시하였다. 총 2,353명을 대상으로 세부 연령 그룹별 정신건강 특성, 식이 섭취수준 및 HRQoL 수준을 비교하였다. 노인 당뇨병 인지자들을 65–69세, 70–74세, ≥ 75세로 분류하여 EQ-5D 개별 항목과 index 수준을 비교 분석한 결과 5가지 항목 중, 연령이 높을수록 운동능력, 자기관리, 일상활동, 통증/불편항목의 수준이 낮은 결과를 보였고, EQ-5D index 또한 가장 낮은 것으로 나타났다. 노인 당뇨병 환자의 경우, 질환을 통해 직접적인 영향을 받는 것과 노화로 인해 나타나는 여러 기능장애들이 함께 동반되는 경우가 많기 때문에 이를 반영한 개별적인 치료 및 관리가 필요한 실정이다. 이에 따라 노인 당뇨병 환자들의 건강관리에서 HRQoL 측정의 중요성은 강조되며, 지속적인 추적 조사를 통해 노인 당뇨병 환자들의 건강에 영향을 주는 특정 요인에 대한 체계적인 관리 및 파악이 가능할 것으로 생각된다. 또한 이를 고려하여 국내 당뇨병 환자의 HRQoL 수준을 향상시키는 것은 개인의 건강관리뿐만 아니라 국가의 보건복지정책에 이바지할 수 있을 것으로 사료된다. Purpose: Although the importance of life satisfaction in patients with chronic diseases has been emphasized, limited studies have been performed on the health-related quality of life (HRQoL) among elderly patients with chronic diseases. This study examined the HRQoL among Korean patients with diabetes aged 65 years or older. Methods: This study used data from the Korea National Health and Nutrition Examination Survey 2008–2016. The analysis included data of 2,353 elderly patients with diabetes aged 65 years or older who were aware that they had type 2 diabetes. Each participant was categorized into one of three age groups (65–69 years, 70–74 years, and ≥ 75 years). The EuroQol-5 Dimensions (EQ-5D) scale was computed to estimate the HRQoL. Multivariate linear regression analysis was used to compare the demographic characteristics, lifestyle characteristics, nutrient intake, and EQ-5D scores between the age groups. Results: The intake levels of most nutrients were similar in the three age groups, but the total energy intake levels were significantly lower, and the sodium intake levels were significantly higher in the older age groups (p < 0.001). In addition, all items of the EQ-5D index, except for anxiety/depression, tended to decrease with age. In particular, the oldest age group (≥ 75 years) showed significantly lower levels on most items and the EQ-5D index than those in the other age groups (p < 0.001). Conclusion: Elderly patients with type 2 diabetes may have low levels of life satisfaction, which worsens with increasing age. Therefore, it is important to develop and manage personalized guidelines and medical systems so that elderly patients have a higher quality of life for the remainder of their lives. The results of this study are expected to be used as basic data for the systematic management of diabetes in elderly Korean patients.