동일한 시료에 대한 국내 기관간의 STR 분석결과 비교 : STR 유전자좌 분석법의 표준화 설정을 위하여

박종태,신경진,양윤석,우광만,이숭덕,이승환,이정빈,정연보,조승희,한길로,한면수,홍승범 大韓法醫學會 2001 대한법의학회지 Vol.25 No.1

This paper described a collaborative exercise intended to see what kinds of short tandem repeat (STR) loci are used in different DNA typing laboratories in Korea and to compare their results for the demonstration whether uniformity of DNA profiling results from different laboratory could be achieved in Korea Laboratories were asked to test five tissue DNAs using methods routinely used in each laboratory and to report the results to the coordinating laboratory. The exercise demonstrated that each laboratory was using different STR loci for the typing with different STR numbers,2 VNTRS,36 STRs and amelogenin in total, and the direct comparison of the results from all the laboratory for the 18 loci could not be done as only one laboratory submitted typing results. Among 21 loci for which several laboratories submitted typing results, results for 14 loci were the same and results for the other 7 loci were different depending on the participating laboratory. D1S80, F13A01, D16S539, D21S11, D18S51, D3S1744 were the loci with different typing results. Even in the cases where commercial kits were used, the results were not the same depending on the machines used, that is the capillary electrophoresis or the gel based electrophoresis. The reason for the different results, points about the standardization of the methods arid the profiling data were described.

메디컬시험기관에서 ISO 15189 도입의 필요성과 시행의 효용성

양만길 ( Man Gil Yang ),이원호 ( Won Ho Lee ),전진현 ( Jin Hyun Jun ) 대한임상검사과학회 2016 대한임상검사과학회지(KJCLS) Vol.48 No.2

다양한 분야에 대한 품질표준들 중에서 메디컬시험기관에 대한 품질 및 적격성에 대한 요구사항인 ISO 15189는 ISO 9001; 품질경영시스템-요구사항과 ISO/IEC 17025; 시험기관 및 교정기관의 능력에 관한 일반요구사항인 두 표준을 바탕으로 메디컬시험기관에 요구되는 사항들을 충족하도록 제정되어 있다. 이 ISO 15189에서는 메디컬시험기관 검사에 관여하는 실무자의 자격 및 지속적인역량, 시험기관 규모, 장비, 시약 및 소모품, 분석전 및 분석인자, 품질보증을 위한 고려사항 및 분석 후 인자 등이 기술되어 있다. 인정과정에서는 업무와 관련된 범주에 전문성을 갖춘 전문가 및 임상 평가사들은 다음의 사항을 포함하여 시험데이터 산출에 영향을 미치는 시험기관에서의 모든 요인에 대한 철저한 평가를 수행한다. 메디컬시험기관의 품질경영시스템은 임상의 또는 환자의 니즈 및 요구사항을 충족시켜야 한다. 품질경영시스템을 기획할 때는 요구사항을 준수하고 품질목표를 충족시켜야 하며 변경하고자 하는 경우에는 품질경영시스템의 통합성을 유지시켜야 한다. 품질경영시스템의 통합성은 목표 달성을 위한 세부 요소들의 상호작용 즉, Plan-Do-Check-Act (P-D-C-A) cycle이 서로 유기적으로 작용하여 지속적인 개선(improvement)이 이루어짐을 의미한다. 메디컬시험기관 ISO 15189 인정제도는 법률 또는 국제기준에 적합한 인정기구가 전문적인 자격을 갖춘 평가사로 하여금 메디컬시험기관의 품질 시스템과 기술능력을 평가하도록 하여 메디컬시험분야에 대한 시험능력이 있다는 것을 공식적으로 인정하는 제도로 우리나라에서는 한국인정기구(KOLAS)가 담당하고 있다. 정부의 2010년 무렵의 KS P ISO 15189 도입 계획은 다소 지연되어, 2016년 4월 1일 기준으로 한국인정기구(KOLAS)를 통해 인정 받은 메디컬시험기관은 공공기관 2곳(한국원자력의학원 생물학적선량 평가실, 서울대학교병원 핵의학과)과 민간기관이 3곳(삼성서울병원 핵의학과, 서울아산병원 핵의학과, 서울아산병원 병리과)뿐이지만, ISO 15189 도입의 궁극적인 목적인 메디컬 서비스의 질적 수준의 향상과 국제적인 경쟁력 강화에 대한 사회적인 요구를 반영하기 위해, 각 기관에서는 적극적인 자세로 도입에 노력해야 할 것으로 생각된다. The requirements for medical laboratories ISO 15189 is examined in organization and a quality management system, stressing the importance of evidence, document control, and control of records and clinical material. Medical services are provided from the areas of resource management, and pre-examination, examination and post-examination processes. The main goal of ISO 15189 accreditation is to improve the quality of laboratory services provided for patients and clinical users not only through compliance with consensually developed and harmonized requirements but also by adopting the philosophy of continual improvement using the Plan-Do-Check-Act cycle. Laboratory quality should be evaluated and improved in all steps of the testing process as the state-of-the art indicates that the pre- and post-analytical phases are more vulnerable to errors than the intra-analytical phase. The Korea Laboratory Accreditation Scheme (KOLAS), a national accreditation body, provides medical laboratory accreditations for appropriate approaches to evaluating the competence of a medical laboratory in providing effective services to its customers and clinical users. Adoption of ISO 15189 in 2010s as a government policy has been delayed, and only 5 laboratories have been accredited to date in Korea. The medical laboratories should seek the adoption of ISO 15189 with a positive attitude for quality improvement and strengthening of international competitiveness.

한국의 결핵환자에서 분리한 결핵균 염색체 DNA의 제한 효소 절단양상 다형성에 관한 연구

양만길(Man-Gil Yang),이연태(Yun-Tai Lee),정규선(Kyu-Sun Jung),김의종(Eui Chong Kim) 大韓微生物學會 1995 大韓微生物學會誌 Vol.30 No.6

Hepatitis B Prophylaxis after Liver Transplantation in Korea: Analysis of the KOTRY Database

Gil-Chun Park,Shin Hwang,김명수,Dong-Hwan Jung,Gi Won Song,Kwang-Woong Lee,Jong Man Kim,Jae Geun Lee,류제호,Dong Lak Choi,Hee-Jung Wang,Bong Wan Kim,Dong-Sik Kim,Yang Won Nah,Young Kyoung You,Koo Jeong Kang 대한의학회 2020 Journal of Korean medical science Vol.35 No.6

Background: Prophylaxis for hepatitis B virus (HBV) recurrence is essential after liver transplantation (LT) in HBV-associated recipients. We conducted real-world analysis of HBV prophylaxis after LT in the Korean population. Methods: Korean Organ Transplantation Registry (KOTRY) database and additionally collected data (n = 326) were analyzed with special reference to types of HBV prophylaxis. Results: The study cohort comprised 267 cases of living-donor LT and 59 cases of deceased-donor LT. Hepatocellular carcinoma (HCC) was diagnosed in 232 (71.2%) of these subjects. Antiviral agents were used in 255 patients (78.2%) prior to LT. HBV DNA was undetectable in 69 cases (21.2%) and detectable over wide concentrations in the other 257 patients (78.8%) prior to LT. Polymerase chain reaction analysis of the store blood samples detected HBV DNA in all patients, with 159 patients (48.9%) showing concentrations > 100 IU/mL. Post-transplant HBV regimens during the first year included combination therapy in 196 (60.1%), hepatitis B immunoglobulin (HBIG) monotherapy in 121 (37.1%), and antiviral monotherapy in 9 (2.8%). In the second post-transplant year, these regimens had changed to combination therapy in 187 (57.4%), HBIG monotherapy in 112 (34.4%), and antiviral monotherapy in 27 (8.3%). Trough antibody to hepatitis B surface antigen titers > 500 IU/mL and >1,000 IU/mL were observed in 61.7% and 25.2%, respectively. The mean simulative half-life of HBIG was 21.6 ± 4.3 days with a median 17.7 days. Up to 2-year follow-up period, HCC recurrence and HBV recurrence developed in 18 (5.5%) and 6 (1.8%), respectively. HCC recurrence developed in 3 of 6 patients with HBV recurrence. Conclusion: Combination therapy is the mainstay of HBV prophylaxis protocols in a majority of Korean LT centers, but HBIG was often administered excessively. Individualized optimization of HBIG treatments using SHL is necessary to adjust the HBIG infusion interval.

객체지향 모듈에서의 단위 테스팅 방법

한길표(Gil-pyo Man),백청호(Cheung-ho Baek),양해술(Hae-sool Yang) 한국정보과학회 1993 한국정보과학회 학술발표논문집 Vol.20 No.1

최근, 문제 도메인으로 구성된 객체를 고려한 객체지향이라는 시스템 분해(decomposition)에 대한 새로운 연구에 관심이 집중되고 있다. 그러나 현재 이 객체지향 패러다임이 소프트웨어 개발에 자주 사용되고 있음에도 불구하고, 객체지향 테스팅에 관한 연구는 거의 미비한 상태에 있다. 종래의 절차형 언어 단위 테스팅으로는 객체지향 모듈을 정확히 테스팅할 수 없기 때문에, 본 연구에서는 객체지향 모듈의 테스팅 시기, 기법, 대상 그리고 테스터가 갖추어야 할 기본 지식에 대하여 간단히 살펴보고, 클래스의 메세지 전송과 계승 및 재사용성이 단위 테스팅에 미치는 영향에 대하여 살펴보기로 한다.

논문 : 전남지역 숲길의 모노테르펜류 농도와 공기질 특성

오길영 ( Gil Young Oh ),서윤규 ( Yun Gyu Seo ),박귀환 ( Gui Hwan Park ),김익산 ( Ik San Kim ),배주순 ( Ju Soon Bae ),박혜영 ( Hye Young Park ),양수인 ( Soo In Yang ),전준민 ( Jun Min Jeon ),정만호 ( Man Ho Jeong ),서윤섭 ( Yun Seo 한국임학회 2012 한국산림과학회지 Vol.101 No.2

본 연구는 숲속 환경대기에서 모노테르펜류(a-Pinene, camphene, sabinene, b-pinene, myrcene, terpinolene, limonene, 1,8-cineol, linalool, camphor)의 농도분포, 숲속 대기의 청정도 및 공기음이온 분포를 파악하기 위해 전남도내 5개 유명 숲을 대상으로 3 계절(봄, 여름, 가을) 동안 수행하였다. 4 시간 평균농도 기준으로 대나무가 우점하고 있는 죽녹원지점에서 b-pinene+myrcene이 958pptv, 우드랜드와 순천만에서 a-pinene(524pptv)과, limonene(445pptv) 이 각각 높은 농도로 나타났다. 계절별로는 우드랜드와 선암사는 봄, 죽녹원과 순천만은 여름에 가장 최고치를 보였다. 대기환경기준물질 농도는 환경정책기본법의 환경기준보다 매우 낮은 농도로 나타났다. 공기음이온은 지점별로 공기 1 mL당 최고 400~3,000개로 조사되었다. 모노테르펜과 공기음이온사이에 유의한 상관은 나타나지 않았다. Three-season field study was conducted to investigate the distribution of monoterpene (10) and air anion in ambient air at five forests. a-Pinene, camphene, sabinene, b-pinene, myrcene, terpinolene, limonene, 1,8-cineol, linalool, and camphor were quantified in the samples collected. The highest 4-hour mean concentration of total monoterpene were b-pinene+myrcene as 958pptv at Juknokwon dominated by bamboo and a-pinene (524pptv), limonene (445pptv) at Wood Land and Suncheon Bay, respectively. The seasonal means were observed to peak during spring with their maximum at Wood Land and Seonam Temple and during summer at Juknokwon and Suncheon Bay. The 24 hour concentration mean of atmosphere standards were found with much lower values than those by law. The highest concentrations of air anion were measured 400~3,000 ea·mL-1 through the sites. There is no significant correlation between the concentration of monoterpene and air anion.

Impact of Recipient Age on Mortality after Liver Transplantation: A Population-Based Cohort Study

( Eunmi Gil ),( Jong Man Kim ),( Jae-won Joh ),( Gee Young Suh ),( Dong Hyun Sinn ),( Kyeongman Jeon ),( Jeong Hoon Yang ),( Jinkyeong Park ) 대한간학회 2017 춘·추계 학술대회 (KASL) Vol.2017 No.1

Aims: Mortality after liver transplantation (LT) depends on many factors. Our aim was to assess short-term outcomes after first LT in a cohort of adult recipients form the national wide database in Korea and identify predictors of in-hospital mortality that could avoid futile LT. Methods: We analyzed data from Health Insurance Review and Assessment Service (HIRA) which is a government-affiliated organization that reviews claim accuracy and assess the quality for the National Health Insurance (NHI) between Aug, 2009 and July, 2014. The patients who had procedural codes of the Korea NHI concerning LT (Q8040-Q8050, Q8140-Q8150) were categorized as patients who received LT. We analyzed the effect of several risk factors on survival by multivariable logistic models. Results: Total 5375 patients had first liver transplantation in this period (1433 received from deceased donor (DD) and 3942 received form living donor (LD)). Mean age was 52.3±8.9 years old and 72.8% (n = 3913) was male. Most common underlying cause of LT was viral hepatitis (46.9 %) due to hepatitis B or C virus, with or without malignant neoplasm of liver. Total 346 patients (6.4%) were died after liver transplantation before hospital discharge. DDLT recipients’ mortality was 14.0% (n = 200) and LDLT recipients’ mortality was 3.7% (n = 146) (p < 0.001). Multiple logistic analysis identified four variables associated with patient survival: recipient age older than 65 years old, recipient on mechanical ventilation more than 3 days, recipients on perioperative hemodialysis, and vasopressor support more than 2 days. Conclusions: Our data shows recipient age, mechanical ventilation, dialysis, and shock had an ability to predict short term outcome. Our results can be used to identify futile cases in which expected outcomes is too poor to justify transplantation.

현미식초가 국화삽수의 발근과 분식국화의 생장에 미치는 영향

양동만(Dong Man Yang),조순주(Soon Ju Jo),박길환(Gil Hwan Park),이정필(Jeong Pil Lee),안규빈(Gyu Bin Ahn),정순주(Soon Ju Chung) 한국원예학회 2002 한국원예학회 학술발표요지 Vol.2002 No.6

Label-Free DNA Chip Using Polydiacetylene Supramolecules

Doo Ho Yang,Eun Jin Kim,Gil Sun Lee,Dong June Ahn,Jong-Man Kim 한국고분자학회 2006 한국고분자학회 학술대회 연구논문 초록집 Vol.2006 No.10

Dip-slide(URICULT)법에 관한 고찰

고영진,양만길,정재원,박정오,장기봉 대한임상병리사협회 1985 대한임상병리사회지 Vol.17 No.1

The bacterial culture was performed as described by Naylor and Guttmann(1967): immediately after taking the specimen the urine was poured on a dip-slide coated with CLED(cystine-Lactose-electrolyte-Deficient) on one side and with MacConkey agar on the other(Uricult). The dip-slides were kept in an incubator overnight and read the following day. We performed a comparative study of standard loop and dip-slide methods. The results obtained from 659 cases of urines were as follows; 1) Standard loop methods. 18hrs at 37℃-Negative 87.1%, doubtful 10.9% and positive 2.0%. 2) Dip-slide URICULT, 18hrs at 15∼20℃-Negative 88.5%, doubtful 9.5% and positive 2.0%. 3) Dip-slide URICULT, 18hrs at l5∼20hrs℃ and 24hrs at 37℃-Negative 88.5%, doubtful 9.5% and positive 2.0%.