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( Feng Yao Wu ),( Feng Ming Zou ),( Jun Qiang Jia ),( Sheng Peng Wang ),( Guo Zheng Zhang ),( Xi Jie Guo ),( Zhong Zheng Gui ) 한국잠사학회 2011 International Journal of Industrial Entomology Vol.23 No.1
Cathepsins are well-characterized proteases that are ubiquitously expressed in lysosomes. Previous work revealed that Bombyx mori cathepsins B and D are expressed in the fat body and undergo decomposition during larval-pupal metamorphosis. Quantitative RTPCR was performed to detect cathepsin gene expression at the transcription level when challenged by B. mori nuclear polyhedrosis virus (BmNPV), temperature and hormones (20-hydroxyecdysone (20E) and juvenile hormone analogue (JHA)). mRNAs encoding cathepsins B and D were significantly enhanced after the larvae were infected with BmNPV, and the peak of the induction appeared at 1 day before spinning. This attenuated the inducing effect on cathepsin expression caused by infection. Temperature shock induced cathepsin expression at the later stage of the 5th instar, and transcription levels varied with development stage and temperature. Cathepsin B and D mRNA expression in the fat body were significantly induced by JHA at the day before spinning, and with 20E, the expression reached a peak at the last day of the 5th instar. Cathepsin B and D mRNA expression exhibited detectable changes post-treatment, without significant differences between or among the hormone concentrations.
The Influence of Challenge on Cathepsin B and D Expression Patterns in the Silkworm Bombyx mori L.
Wu, Feng-Yao,Zou, Feng-Ming,Jia, Jun-Qiang,Wang, Sheng-Peng,Zhang, Guo-Zheng,Guo, Xi-Jie,Gui, Zhong-Zheng Korean Society of Sericultural Science 2011 International Journal of Industrial Entomology Vol.23 No.1
Cathepsins are well-characterized proteases that are ubiquitously expressed in lysosomes. Previous work revealed that $Bombyx$ $mori$ cathepsins B and D are expressed in the fat body and undergo decomposition during larval-pupal metamorphosis. Quantitative RT-PCR was performed to detect cathepsin gene expression at the transcription level when challenged by $B.$ $mori$ nuclear polyhedrosis virus (BmNPV), temperature and hormones (20-hydroxyecdysone (20E) and juvenile hormone analogue (JHA)). mRNAs encoding cathepsins B and D were significantly enhanced after the larvae were infected with BmNPV, and the peak of the induction appeared at 1 day before spinning. This attenuated the inducing effect on cathepsin expression caused by infection. Temperature shock induced cathepsin expression at the later stage of the $5^{th}$ instar, and transcription levels varied with development stage and temperature. Cathepsin B and D mRNA expression in the fat body were significantly induced by JHA at the day before spinning, and with 20E, the expression reached a peak at the last day of the $5^{th}$ instar. Cathepsin B and D mRNA expression exhibited detectable changes post-treatment, without significant differences between or among the hormone concentrations.
Wu, Yao,Wu, Guorong,Wang, Li,Munsell, Brent C.,Wang, Qian,Lin, Weili,Feng, Qianjin,Chen, Wufan,Shen, Dinggang Wiley (John WileySons) 2015 Medical physics Vol.42 No.7
<P>To investigate anatomical differences across individual subjects, or longitudinal changes in early brain development, it is important to perform accurate image registration. However, due to fast brain development and dynamic tissue appearance changes, it is very difficult to align infant brain images acquired from birth to 1-yr-old.</P>
Zhang, Peng,Jin, Yue-Fei,Zhang, Qiao,Wu, Yi-Ming,Wu, Wei-Dong,Yao, Wu,Wu, Yong-Jun,Li, Zhi-Tao,Zhao, Yong,Liu, Yu,Feng, Fei-Fei Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.12
Background: We sought to evaluate the role of tumor associated macrophages (TAMs) on the promotion of coal tar pitch extract (CTPE)-induced tumorigenesis of human bronchial epithelial cells (BEAS-2B) and tumor metastasis in nude mice, and related mechanisms. Materials and Methods: BEAS-2B cells were first treated with 2.4 mg/mL CTPE for 72 hours. After removal of CTPE, the cells were continuously cultured and passaged using trypsin-EDTA. THP-1 cells were used as macrophage-like cells. BEAS-2B cells under different conditions (n=6/group) were injected into the back necks of nude mice, and alterations of tumor xenograft growth, indicative of tumorigenicity, and tumor metastasis were determined. Pathological changes (tumor nests and microvascular lesions) of HE-stained tumor tissues were also evaluated. The expression of AP-1(c-Jun) in xenografts and metastatic tumors was determined using immunohistochemistry. Results: Tumor size and weight in nude mice transplanted with the mixture of CTPE-induced passage 30 BEAS-2B and THP-1 cells (2:1) were increased compared to those from the CTPE-treated BEAS-2B cells at passage 30 alone at different observation time points. Tumor metastasis to lymph nodes and liver was only detected after transplantation of a mixture the two kinds of cells. The numbers of tumor nests and microvascular lesions, and the expression levels of AP-1 (c-Jun) in tumors from the mixture of two kinds of cells were increased apparently in contrast to those in tumor from the CTPE-treated BEAS-2B cells of passage 30 alone. In addition, there was positive correlation between AP-1 (c-Jun) expression level and the number of microvascular lesions, or between AP-1 (c-Jun) expression level and tumor metastasis in these two groups. Conclusions: TAMs not only facilitate tumorigenesis transformation of CTPE-induced BEAS-2B cells, but also promote tumor growth, angiogenesis and metastasis in nude mice in vivo, which may be mediated by AP-1.
Wu Huiying,Wu Guiying,Yao Ping,Zhou Yan,Zhang Feng,Zuo Baoqi 한국고분자학회 2018 폴리머 Vol.42 No.5
In this work, silk fibroin (SF) filament with fibrils regenerated by dissolving in CaCl₂-formic acid (FA) was prepared by wet-spun method at room temperature. Different from traditional dissolved methods, SF solutions obtained by dissolving in CaCl₂-FA preserved fibrils, which have been recognized as the key to the high performance of native silk. The morphology of SF filament was analyzed, very dense filaments with smooth surface and circular, nanofibrils could be observed in longitudinal and cross-sections of filaments. Moreover, the breaking stress of samples was gradually increased with the increase of draw-down ratios. After 3 times drawing, the breaking stress and elongation at break of filament were 276.4±22.6 MPa and 40.8±3.1%, respectively. At the same time, the secondary structure of SF filament was typical β-sheet. In addition, SF filaments showed excellent degradation property, the mass lost of SF filament declined 42% after incubating in protease XIV solution. Above all, the human mesenchymal stem cells (hMSCs) adhered very well on the surface of the filaments, which demonstrated the good biocompatibility of SF filaments, was suitable for application in tissue engineering.
Enhancing Effects of NaHSO<sub>3</sub> on Corrosion of T91 Steel
Wu, Tangqing,Tan, Yao,Wang, Jun,Xu, Song,Liu, Lanlan,Feng, Chao,Yin, Fucheng The Korean Electrochemical Society 2020 Journal of electrochemical science and technology Vol.11 No.4
In the paper, corrosion behavior of T91 steel in different concentrations of NaHSO<sub>3</sub> solution was studied in combination with scanning electron microscope (SEM) and electrochemical measurements. The results showed that the steel exhibited active anodic dissolution characteristics in the solution, and NaHSO<sub>3</sub> concentration affected both cathodic and anodic behaviors. The steel surface was covered by intact corrosion products in the solutions, but the compactness and mechanical properties of the corrosion products degraded with the increase of NaHSO<sub>3</sub> concentration. In low-concentration NaHSO<sub>3</sub> solution the steel tended to undergo uniform corrosion with slight corrosion pits, but its corrosion mode gradually transited to localized corrosion as the NaHSO<sub>3</sub> concentration increased. The mechanical property degradation of the corrosion products caused by sulfur compounds and the pH decrease of the solution are the important factors to accelerating its corrosion process.
Influence of Spatial Distribution of Pores on NMR Transverse Relaxation Time in Pebbly Sandstone
Feng Wu,Yanping Xi,Qicheng Fan,Cong Yao,Linlin Cong,Fengsheng Zhang,Yan Kuang 한국자기학회 2019 Journal of Magnetics Vol.24 No.4
The relationship between transverse relaxation time (T₂) and pore size distribution is the basis of NMR applications for rocks. However, the equations for T₂ are not accurate enough in rocks with complicated pore structures. Taking pebbly sandstone from the northwestern Junggar Basin in China as an example, the aim of this study is to discover the spatial distribution of pores and its influence on T₂. Porosity, permeability, micro-images and T₂ distributions were acquired from rock samples, and pore structure parameters were obtained from binarized thin section images. The results show that as the grain size increases, the proportion of dissolution pores increases and the spatial distribution of pores changes from a random to a clustered pattern. The relaxation of a hydrogen atom takes longer and T₂ is higher in dissolution pores compared with those in intergranular pores. New equations for T₂ that consider the spatial distribution of pores are proposed.
A helix slope deviation compensation model for milling small modulus copper electrode gears
Zhipeng Feng,Zhao-Yao Shi,Shoujin Lin,Aijun Tong,Peng Wang,Guoqing Wu 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.7
When a milling tool is used to mill small modulus copper electrode gears (SMCEG), the deformation is inevitable, thus leading to the helix slope deviation (HSD) of copper electrode gears (CEG). To compensate the influence of milling tool deflection (MTD) on the HSD, a universal HSD compensation model was established for different sizes of milling tools. First, using the principle of orthogonal experiment, a linear regression model of milling parameters and milling tool radius is established. Secondly, the average value of the HSD obtained by the gear measuring instrument is converted into the maximum of MTD according to the principle of helix line formation. The maximum deflections of the milling tools are used as the response value of the regression analysis to fit the maximum deflection curve of the milling tool. Through the reverse adjustment of the rotation angle of the rotating motor, the HSD compensation is realized. Finally, the model was verified by the milling tests. The results showed that the helix deviation reached the requirements of level 4 to 5 in GB/T 38192-2019, and proved the correctness of the established MTD model. The model is significant for milling SMCEG.
Scaling up the in-hospital hepatitis C virus care cascade in Taiwan
( Chung-feng Huang ),( Pey-fang Wu ),( Ming-lun Yeh ),( Ching-i Huang ),( Po-cheng Liang ),( Cheng-ting Hsu ),( Po-yao Hsu ),( Hung-yin Liu ),( Ying-chou Huang ),( Zu-yau Lin ),( Shinn-cherng Chen ),( 대한간학회 2021 Clinical and Molecular Hepatology(대한간학회지) Vol.27 No.1
Background/Aims: Obstacles exist in facilitating hepatitis C virus (HCV) care cascade. To increase timely and accurate diagnosis, disease awareness and accessibility, in-hospital HCV reflex testing followed by automatic appointments and a late call-back strategy (R.N.A. model) was applied. We aimed to compare the HCV treatment rate of patients treated with this strategy compared to those without. Methods: One hundred and twenty-five anti-HCV seropositive patients who adopted the R.N.A. model in 2020 and another 1,396 controls treated in 2019 were enrolled to compare the gaps in accurate HCV RNA diagnosis to final treatment allocation. Results: The HCV RNA testing rate was significantly higher in patients who received reflex testing than in those without reflex testing (100% vs. 84.8%, P<0.001). When patients were stratified according to the referring outpatient department, a significant improvement in the HCV RNA testing rate was particularly noted in patients from non-hepatology departments (100% vs. 23.3%, P<0.001). The treatment rate in HCV RNA seropositive patients was 83% (83/100) after the adoption of the R.N.A. model, among whom 96.1% and 73.9% of patients were from the hepatology and non-hepatology departments, respectively. Compared to subjects without R.N.A. model application, a significant improvement in the treatment rate was observed for patients from non-hepatology departments (73.9% vs. 27.8%, P=0.001). The application of the R.N.A. model significantly increased the in-hospital HCV treatment uptake from 6.4% to 73.9% for patients from non-hepatology departments (P<0.001). Conclusions: The care cascade increased the treatment uptake and set up a model for enhancing in-hospital HCV elimination. (Clin Mol Hepatol 2021;27:136-143)