Aptamer‑linked immobilized sorbent assay for detecting GMO marker, phosphinothricin acetyltransferase (PAT)

Woo‑Ri Shin,Mun‑Jong Lee,SEKHONSIMRANJEET SINGH,Ji Hun Kim,Sun Chang Kim,Byung‑Kwan Cho,Ji‑Young Ahn,Yang‑Hoon Kim 대한독성 유전단백체 학회 2020 Molecular & cellular toxicology Vol.16 No.3

Background Development of genetically modified crops has rapidly increased in last few years. The most widely grown GM crops express genes that confer herbicide tolerance and insect resistance. Detection system of GM crops is important for safety evaluation before its consumption. Objective The purpose of this research is to detect GM crops, especially PAT, in food-samples. Results The bar gene (PAT protein, herbicide resistant) was cloned in pGEX-4T-1 and expressed by E. coli. The high-affinity PAT-specific single-stranded DNA (ssDNA) aptamers were obtained from a random DNA library. MOE docking study was performed to identify the potential binding region of the selected aptamers on PAT. Aptamer-linked immobilized sorbent assay (ALISA) method was used to detect PAT. Conclusion We screened aptamer against PAT for developing an efficient detection method. The selected PAT specific aptamers, HRPA-05 and HRPA-07, showed the distinct target binding behaviors, and detected PAT protein by aptamer-linked immobilized sorbent assay method with high efficiency and selectivity.

전남 진도 지역어의 단모음 실현 양상

하영우 ( Ha Young-woo ),신우봉 ( Shin Woo-bong ) 고려대학교 민족문화연구원 2018 민족문화연구 Vol.79 No.-

The aim of this study was to examine the acoustic characteristics of the monophthongs in the Jeonnam-Jindo dialect. For this purpose, the experiment was conducted and data was gathered from Jindo dialect speakers in their 10s and 5,60s. The data were analyzed on the basis of the subjects’ generation, gender. The result of production experiment was that Jindo dialect speakers have same vowel system between generations: 7 vowel system (/i, E, a, □, i, u, o/). The older generation of Jindo speakers mostly pronounce /ㅚ/ and /ㅟ/ as monophthong irrespective of sex and the young generation of Jindo speakers entirely pronounce /ㅚ/ and /ㅟ/ as monophthong. Also, we found that /Щ/, /u/ moved toward front and /o/ moved toward high in the vowel space from the Jindo speakers in their older to those in their young. And /Λ/ showed a shift in the vowel space from the back to the open vowel. The results revealed a chain-like shift in the high and mid back vowels. It is assumable that the degree of standardization appearing in the place of articulation on vowel is quite high in the Jindo dialect. Previous studies discussed the Jindo vowel system on the basis of the observations made by the researchers. The current study, on the other hand, is significant in that it investigated sound changes in vowel production throughout the vowel system and confirmed the articulation place of vowel using phonetic methods.

야뇨증 환아들의 심리사회적 특성에 대한 다기관 연구 : 행동 및 정서 문제를 중심으로

조수철,김재원,신민섭,황준원,한상원,박관현,이상돈,김경도,김건석,서홍진,이유식,정재용,김영균,문두건,남궁미경,한창희,조원열,김영식,배기수,이종국,정우영,신의진 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.6

Objectives : The aim of this study was to examine the behavioral and emotional problems associated with nocturnal enuresis in Korean children. Methods : Three hundred eighteen children with nocturnal enuresis, together with their parents, completed the Child Behavior Checklist (CBCL), Disruptive Behavior Disorder Scale according to DSM-IV (DBDS), Children's Depression Inventory (CDI), State-Trait Anxiety Inventory for Children (STAIC), and Piers-Harris Children's Self-Concept Scale (PHCSC). Ninety-three normal students were selected as the control group. Results : Compared to the normal control group, the mean scores with regard to the withdrawn, social problems, attention problems, delinquent behavior, aggressive behavior, externalizing problems and total problems profiles were significantly higher in the nocturnal enuresis group according to the CBCL results. The nocturnal enuresis group also scored significantly higher in the ADHD and ODD profiles of the DBDS. The nocturnal enuresis group was more depressed and anxious than the control group according to the results of the CDI and STAI. The mean score of the PHCSC was significantly lower in the nocturnal enuresis group when compared to the normal control group. Conclusion : The results of this study suggest that children with nocturnal enuresis in Korea have clinically relevant behavioral and emotional problems. The findings support the link between nocturnal enuresis and psychopathology in Korean children.

한의진단명과 진단요건의 표준화 연구 II (표준화 실례) : 2차년도 연구결과 중간 보고

양기상,최선미,최승훈,안규석,박경모,박종현,김성우,신승호,정우열,전병훈,고현,김정범,신상우,김성훈,김동희,권영규,엄현섭,장혜옥 한국한의학연구원 1996 한국한의학연구원논문집 Vol.2 No.1

The diagnostic requirements were suggested and explained regarding the systems of differentiation of symptoms and signs in the second year study of standardization and unification of the terms and conditions used for diagnosis in oriental medicine. The systems were as follows; - differential diagnosis according to condition of body fluid, differentiation of syndromes according to the state of qi and blood, differential diagnosis according to relative excessiveness or deficiency of yin and yang(氣血陰陽津液辨證) - differentiation of diseases according to pathological changes of the viscera and their interrelation - analyzing and differentiating of febrile diseases in accordance with the theory of the six channels(傷寒辨證) The individual diagnosis pattern was arranged by the diagnostic requirements in the following order : another name(異名), notion of diagnosis pattern, index of differentiation of symptoms and signs(辨證指標), the main point of diagnosis, analysis of diagnosis pattern(證候分析), discrimination of diagnosis pattern(證候鑑別), a way of curing a diseases(治法), prescription(處方), herb in common use(常用藥物), diseases appearing the diagnosis pattern(常見疾病), documents(文獻調査). This study was carried out on the basis of the Chinese documents and references.

콩첨가 증편의 미생물 변화와 품질 특성

우경자,신광숙,한영숙 동아시아식생활학회 1998 동아시아식생활학회지 Vol.8 No.2

The effect of soybean addition to the preparation of Jeung-Pyun(Korean fermented rice cake) and the fermentational characteristics of Jeung-Pyun dough were determined. Jeung-Pyun dough composed of rice 100g, soybean 20g, water 40g, salt 0.8g, sugar 15g and Tak-ju 30g was fermented for up to 20 hours. The numbers of total microbes, yeasts and lactic acid bacteria in the dough during fermentation increased rapidly during 5 hours of fermentation, but Leuconostoc mesenteroides during 7 hours of fermentation. Generally, there were many more microbes in the Jeung-Pyun dough with soybeans than in the Jeung-Pyun dough with rice. The volume of 7 hour fermented Jeung-Pyun was most massive especially in the soybean-added Jeung-Pyun. In 1% dextran-added rice Jeung-Pyun, the volume of steamed cake was increased more than that of rice Jeung-Pyun, but it was not as bulky as in the soybean-added cake. In the sensory evaluation of the test cake products, the 7 hour fermented cake was preferred by most. As for instrumental characteristics, the 7 hour fermented Jeung-Pyun showed the lowest values for hardness and elasticity, brittleness and chewiness. The rice Jeung-Pyun showed higher values for hardness and brittleness than in soybean-added Jeung-Pyun. When the Jeung-Pyuns were stored at 20℃, the hardness, brittleness and cohesiveness of rice Jeung-Pyun were respectively increased but not in the soybean-added Jeung-Pyun. However, the hardness of rice Jeung-Pyun kept at 4℃ increased rapidly after 5 days of storage. The cohesiveness, elasticity, brittleness, chewiness, gumminess of both rice Jeung-Pyun and soybean-added Jeung-Pyun were decreased with storage time.

총담관결찰이 담세관에 미치는 영향에 관한 투과 및 주사전자현미경적 연구

최경우,신영철 대한해부학회 1994 Anatomy & Cell Biology Vol.27 No.4

타목시펜이 간세포암 세포주의 성장과 Transforming Growth Factor-β 1의 발현에 미치는 영향

신정우,정영화,박무인,김정아,최민희,이윤정,류수형,박능화,이한주,이영상,서동진,유은실 대한간학회 2003 Clinical and Molecular Hepatology(대한간학회지) Vol.9 No.1

목적: 타목시펜을 진행성 간세포암 환자에게 투여한 결과 일부에서 효과가 있었다는 보고가 있으나 타목시펜이 간세포암의 성장을 억제하는 기전은 밝혀져 있지 않다. TGF-β1은 간세포암의 성장과 분화에 관여하는 중요한 성장인자로 알려져 있으며 최근 타목시펜이 TGF-β1의 분비조절에도 관여한다는 보고들이 있었다. 따라서 본 연구에서는 타목시펜이 간세포암의 성장과 TGF-β1의 발현에 미치는 영향을 알아보고 궁극적으로 타목시펜이 간세포암 환자에게 항암제로 사용될 수 있는지를 알아보고자 하였다. 대상과 방법: 인간 간세포암 세포주인 Hep 3B세포를 에스트로겐이 없는 RPMI 1640과 dextran coated charcoal로 처리한 5% fetal bovine serum을 혼합한 배지에서 3일 배양 후 2×10^4 세포/well로 분주하여 2일간 배양한 후 0.1 μM, 0.5 μM, 1 μM, 5 μM, 10 μM 농도의 타목시펜으로 처치하고 6일간 배양하였다. 매일 세포를 수집하여 trypan blue로 염색한 후 생존 세포수를 산출하였으며 상층액내 TGF-β1 농도는 ELISA법으로 측정하였다. 결과: 비교적 저 농도인 0.1 μM 타목시펜 처치군은 배양 6일째 생존 세포수가 대조군에 비해 의미 있게 증가하였다(2.59×10^6 vs 1.97×10^6, p<0.05). 생존 세포수는 타목시펜농도가 증가할수록 감소하여 10 μM 처치군은 대조군에 비해 의미있는 감소를 보였다(1.4×10^6 vs 1.97×10^6., p<0.05). 상층액내의 TGF-β1dml 분비량은 타목시펜 처치 농도와 상관없이 전 군에서 대조군에 비해 의미있게 감소하였으며 타목시펜 농도에 따른 각 군간에 차이는 없었다. 결론: 타목시펜은 실험실내에서 처치 농도에 따라 간세포암 세포주의 성장에 미치는 영향이 다양했으나, 처치 농도와 관계없이 일정한 정도로 TGF-β1의 발현을 억제하였다. 타목시펜의 이러한 효과는 생체에서 TGF-β1이 과발현된 간세포암의 성장과 진행을 억제할 것으로 생각된다. 또한 타목시펜은 TGF-β1의 발현을 조절하는 기전 이외의 다른 기전을 통해서는 간세포암의 성장을 억제하는 것으로 사료된다. Background/Aims: Tamoxifen has been tried in patients with hepatocellular carcinoma (HCC), however, its inhibitory mechanism remains unknown. In this study, we evaluated the effects of tamoxifen on HCC cell growth and the expression of transforming growth factor-β1 (TGF-β1) which had been known as an important cytokine in growth of HCC. Methods: Hep 3B cells were cultivated in estrogen free media with 0.1 μM, 0.5 μM, 1 μM, 5 μM, and 10μM of tamoxifen for 6 days. Viable cells were counted daily and the TGF-β1 concentrations in supernatant were measured by ELISA method. Results: The number of viable HCC cells increased rather significantly after the treatment of tamoxifen of lower concentration (0.1 μM) compared with that of the control (2.57×10^7 us. 1.97×10^7; p<0.05). As the concentration of treated tamoxifen was higher, the number of viable HCC cells became gradually less, resulting in the significant decrease of it at the highest concentration (10 μM) compared with that of the control (1.40×10^7 us. 1.97×10^7; p<0.05). TGF-β1 concentration in supernatant of tamoxifen-treated samples was significantly decreased compared with those of controls, regardless of the amount of treated tamoxifen. Conclusions: These results suggest that tamoxifen may suppress TGF-β1 expression to an extent, although it has different effects on the proliferation of HCC cells, at the various concentrations of this agent in vitro. Such effects of tamoxifen on TGF-β expression may inhibit the growth and progression of HCCs over-expressing TGF-β1 in vivo.

卓球選手의 競技內容에 關한 分析硏究

申甲浩,洪榮佑 明知大學校 自然科學硏究所 1984 자연과학논문집 Vol.2 No.-

In this study I could get a few results as follows from making out the couses of gains and loses of games acconding to batting tachnique selected from several semifinal games of players porticipotel in the Second Seoul International table tennis game, and from comporing and analyzing also the ralation to gaining and losing -points depending in the effctiv e methods and any other technique in controlling the game management. 1. We can easily find and so I could attain the average contents, s follows, of each player's game of gaining and losing points and ball dropping paints according to the technique of batting balls. A)Mr. Park's stroks number amaunted to 34.91 in all of his games, the forehand's 23.95 strokes were more often than backhand by 13 strokes, and forehand strokes gained 12.98, lose 10.97 and backhand strokes gained 4.32, and losed 6.04 The most major couse of gaining points was "cutting" balls(6.66)and that of losing points was "Smashing'(4.16). B)Eric Boggan stroked the balls 41.5 times in tatal which contain 16.5 strokes of backhand and 25 strokes forehand, and he losed 16.50 points in back strokes and gained 15 points, and his forehand smashing strokes made him enable to gain most of points of them by 4 times,.on the other hand, losed by short of back strokes of 4 times. C)Mr. Waldner stroke balls 41.5 time in all forehand strokes 28.5 time was more than backhand strokes and his main coerse of gaining points was 15.5 time of forestrok and was more than 6.5 times of backstrokes and alsed points by forestrokes in 13 times, while backstrokes make him lose points in 6.5 times and he could have same points gained and losed in using the backhand strokes, while forehand strokes gained more points 15.5 to 13 points We could find him get more points by the same forhand Driving. D)The total stroke number of Maehara Amounted to 34.58 times. Forehandstrokes were 29.28 times . and backhandstrokes only 5.3 time And he losed more points in forehandstrokes (gained 13.97 points losed 15.31 points)And the fact, that he mainly gained by using the backhandstrokes by gaing 3.31 points to 1.99 losing points, may be colled peticular phenomenon. His 'Forehand spin euabled him get more points by getting 7.66 times and he losed points 8.66 times by using Forehand spin method. E)Miss Shin strokes balls 41 times in all and she mostly used Forehandstroke 33 times and there were no deffernces in gaining and losing points in 16.5 time whether she used Fore or Backhandstrokes, But she mainly get points by Forehadn Smashing of 7 times and losed points by Forehand spining. F)Mis Lee's stroke number were Cmporably loss than other players and amounted to 32.28 time in all her Forehandstroke came up to 28.63 times, but losed more points by it than gainded by 11.32 to 17.3 points and also in Backgandstroke gained 0.99 time and losed 3.66 time, Her main skill was Forehand spin dn it mde her more points in 8 times, and lose times by skill. G) Wada's tatal strokes amounted to 36.2 time and Backhand strokes number were of 22.6 time which shows more strokes than 13.6 time of Forehandstrokes, and it can be side the only phenomenon of Miss Wada, she could get paints more often by Backhandstroke than by Forehandstroke(Forehand 9 to Backhand 12)she also losed more points by Backhand( fore 4.60 to Back 10.6). Her favorite skills, back long-cut (5times)and Both -cut (5 times) enabled him equally gain most o points and mostly she losed points by Bachand long-cut,(5.8).

결정화 공정을 위한 L-ornithine aspartate의 용해도에 관한 연구

신동명,김용욱,김우식 慶熙大學校 1998 論文集 Vol.27 No.-

Study on the solubility of L-ornithine aspartate with temperature, alcohol, additive was invescated. Generally, the solubilities of amio acids are effected by orniganic solvent, so the solubility of L-ornithine aspartate was represented to reduce by adding alcohol. The solubility of L-ornithine aspartate with temperature incressed at methanol volume ratio 40%, but was slightly effected at methanol volume ratio 60% and 80%. The effect of aspartic acid was not signifigant, too.

Ornthine-aspartate의 용해도 및 Drowning-out 결정화 연구

신동명,김우식,김용욱 경희대학교 산학협력기술연구원 1997 산학협력기술연구논문집 Vol.3 No.-

오르니틴-아스파테이트의 용해도와 drowning-out결정화에 관한 연구를 수행하였다. 오르니틴-아스파테이트 용액에 알코올을 첨가하면 오르니틴-아스파테이트의 용해도가 극적으로 감소하며, 결과적으로 오르니틴-아스파테이트의 침전이 일어난다. 오르니틴-아스파테이트 침전공정에서 오르니틴-아스파테이트의 회수율과 결정크기는 주입속도, 교반속도 및 주입방식에 크게 영향을 받았다. 또한, 물과 알코올의 혼합액에서 오르니틴-아스파테이트의 용해도는 알코올 분율이 증가함에 따라 감소하는 것으로 나타났다. 알코올-물 혼합용액에서 오르니틴-아스파테이트의 용해도는 Cohn식과 Wilson식들에 의해 성공적으로 예측될 수 있다. Study on solubility and drowning-out crystallization of ornithine-aspartate was carried out. Addition of alcohol to ornithine-aspartate solution reduces dramatically the solubility of ornithine-aspartate and results in the precipitation of ornithine-aspartate. In the process of ornithine-aspartate precipitation the recovery and crystal size of the ornithine-aspartate were significantly affected by the feeding rate , agitation rate and feeding mode. In water and alcohol mixture, the solubility of ornithirne-aspartate was also presented to be reduced as increasing alcohol fraction. The solubility of ornithine-aspartate in solution of alcohol-water mixture can be successfully predicted by Cohn and Wilson equations.