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Comparison of stomach microRNA transcriptomes of Tibetan and Yorkshire pigs by deep sequencing
Wen‑kui Sun,Yanyue Li,Chi Cheng,Yi‑hui Chen,Kai Zeng,Xiaohui Chen,Yiren Gu,Rui Liu,Xuebin Lv,Rong Gao 한국유전학회 2018 Genes & Genomics Vol.40 No.9
MiRNAs regulate the expression of target genes in diverse cellular processes and hence play important roles in different physiological processes, yet little is known about the stomach microRNAome (miRNAome) of the Tibetan pig. The objective of this experiment was to investigate differentially expressed stomach miRNAs participating in digestion. Firstly, we isolated total RNA by Trizol reagent from three Tibetan and three Yorkshire purebred pigs stomach samples at 90-day-old. Secondly, a comprehensive analysis of Tibetan and Yorkshire pig stomach miRNAomes was performed by small RNA sequencing in the Illumina HiSeq 2000 system. Finally, SYBR Green Real-time RT-PCR was performed to validate the differentially expressed miRNAs. We identified 318 unique miRNAs, 260 were co-expressed in both libraries, 17 and 31 miRNAs were specifically expressed in Tibetan and Yorkshire pigs respectively. Fifty six differentially expressed miRNAs were identified by the identifying differentially expressed genes 6 (IDEG6). Kyoto encyclopedia of genes and genomes analysis revealed that some of the differentially expressed miRNAs were associated with protein and fat digestion. Two differentially expressed miRNAs (miR-214-3p and ssc-un39) participating in the digestion of lipid were identified. Additionally, qRT-PCR results suggested that a higher expression of miR-214-3p in the Tibetan pig stomach could lead to relatively lower expression of calcium-dependent phospholipase A2, which is an enzyme important for the digestion of glycerol phospholipid. This study has delineated the different stomach miRNAs expression patterns of Tibetan and Yorkshire pigs, which would help explain the regulatory mechanisms of miRNAs in digestion of Tibetan pigs, and contribute to utilize a the unique digestion merits of Tibetan pig in future porcine hybridization breeding.
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Prevention of Natural Flowering in Pineapple (Ananas comosus) by Shading and Urea Application
Meng-Tzu Lin,Alfred Ming Chen1,Tzong-Shyan Lin,Ching-San Kuan,Ching-Lung Lee,Wen-Ju Yang 한국원예학회 2015 Horticulture, Environment, and Biotechnology Vol.56 No.1
The year-round production of pineapple (Ananas comosus var. comosus) is often interrupted by natural flowering during winter in Taiwan. A stable and promising technique for preventing natural flowering is required. In this study, we have tested the effect of shading and urea application on the flowering of pineapple. Shading 90% of sun light(s) before mid-November delayed the natural flowering of pineapple and the delay was affected by the plant age. For pineapples planted less than 11 month before mid-November, applying 1% urea plus shading treatment (SN) completely inhibited natural flowering. All the flowering-inhibited plants could be forced to flower by applying calcium carbide. Long-term shading might result in a decreased number of fruitlets within a fruit. However, 6 weeks of recovery before forcing flowering was sufficient for plants to produce fruits with quality equal to that of the control plants. In winter, pineapple plants that were prone to flowering tended to accumulate more leaf starch and increase their C/N ratio. The SN treatment increased the leaf nitrogen concentration and thus effectively maintained a low leaf C/N ratio. Furthermore, flowering-inhibited plants exhibited a constantly low level of leaf starch during the winter and their flowering forced by calcium carbide did not accompany with leaf starch accumulation.
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Chen, Wen-Kwei,Liu, Ingrid Y,Chang, Ya-Ting,Chen, Yong-Cyuan,Chen, Chih-Cheng,Yen, Chen-Tung,Shin, Hee-Sup,Chen, Chien-Chang The Society 2010 The Journal of neuroscience Vol.30 No.31
<P>Treatments for chronic musculoskeletal pain, such as lower back pain, fibromyalgia, and myofascial pain syndrome, remain inadequate because of our poor understanding of the mechanisms that underlie these conditions. Although T-type Ca(2+) channels (T-channels) have been implicated in peripheral and central pain sensory pathways, their role in chronic musculoskeletal pain is still unclear. Here, we show that acid-induced chronic mechanical hyperalgesia develops in Ca(v)3.1-deficient and wild-type but not in Ca(v)3.2-deficient male and female mice. We also show that T-channels are required for the initiation, but not maintenance, of acid-induced chronic muscle pain. Blocking T-channels using ethosuximide prevented chronic mechanical hyperalgesia in wild-type mice when administered intraperitoneally or intracerebroventricularly, but not intramuscularly or intrathecally. Furthermore, we found an acid-induced, Ca(v)3.2 T-channel-dependent activation of ERK (extracellular signal-regulated kinase) in the anterior nucleus of paraventricular thalamus (PVA), and prevention of the ERK activation abolished the chronic mechanical hyperalgesia. Our findings suggest that Ca(v)3.2 T-channel-dependent activation of ERK in PVA is required for the development of acid-induced chronic mechanical hyperalgesia.</P>
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Ci-Wen Luo(Ci-Wen Luo),Yu-Hsiang Kuan(Yu-Hsiang Kuan),Wen-Ying Chen(Wen-Ying Chen),Chun-Jung Chen(Chun-Jung Chen),Frank Cheau-Feng Lin(Frank Cheau-Feng Lin ),Stella Chin-Shaw Tsai(Stella Chin-Shaw Tsa 한국역학회 2023 Epidemiology and Health Vol.45 No.-
OBJECTIVES: This cohort study investigated the correlation between Parkinson’s disease (PD) risk and chronic obstructive pulmonary disease (COPD) risk under particulate matter with an aerodynamic diameter ≤2.5 μm (PM2.5) exposure. METHODS: Data from the National Health Research Institutes of Taiwan were used in this study. The Environmental Protection Administration of Taiwan established an air quality monitoring network for monitoring Taiwan’s general air quality. COPD was indicated by at least 3 outpatient records and 1 hospitalization for COPD. After the implementation of age, sex, and endpoint matching at a 1:4 ratio, 137 patients and 548 patients were included in the case group and control group, respectively. Based on the 2005 World Health Organization (WHO) standards, monthly air particle concentration data were classified into the following 4 groups in analyses of exposure–response relationships: normal level, and 1.0, 1.5, and 2.0 times the WHO level ([concentration ≥2]×25 μg/m3×number of exposure months). RESULTS: A multivariate logistic regression revealed that the 1.0 and 1.5 WHO level groups did not significantly differ from the normal level group, but the 2.0 WHO level did (odds ratio, 4.091; 95% confidence interval, 1.180 to 14.188; p=0.038). CONCLUSIONS: Elevated PM2.5 concentrations were significantly correlated with an increased risk of PD among patients with COPD. Furthermore, exposure to high PM2.5 levels can further increase the risk of PD.
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Wei-Che Lin,Wen-Chieh Chen,Pei-Wen Wang,Yi-Chia Chan,Yen-Hsiang Chang,Harn-Shen Chen,Szu-Tah Chen,Wei-Chih Chen,Kai-Lun Cheng,Shun-Yu Chi,Pi-Ling Chiang,Chen-Kai Chou,Feng-Fu Chou,Shun-Chen Huang,Feng 대한초음파의학회 2023 ULTRASONOGRAPHY Vol.42 No.3
Radiofrequency ablation (RFA) is a minimally invasive management strategy that has been widely applied for benign and recurrent malignant thyroid lesions as an alternative to surgery in Taiwan. Members of academic societies for specialists in interventional radiology, endocrinology, and endocrine surgery collaborated to develop the first consensus regarding thyroid RFA in Taiwan. The modified Delphi method was used to reach a consensus. Based on a comprehensive review of recent and valuable literature and expert opinions, the recommendations included indications, pre-procedural evaluations, procedural techniques, post-procedural monitoring, efficacy, and safety, providing a comprehensive review of the application of RFA. The consensus effectively consolidates advice regarding thyroid RFA in clinical practice for local experts.
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Wen-Hua Chen,Chao-Yu Li,Tsui-Ying Chang 한국응용곤충학회 2016 Journal of Asia-Pacific Entomology Vol.19 No.1
The litchi spider mite Oligonychus litchii Lo & Ho is one of the major pests of fruit crops in Taiwan. This study investigated the life history of the litchi spider mite on wax apple (Syzygium samarangense Merr. & Perry) at five constant temperatures (16, 20, 25, 30, and 32 °C) under 70 ± 5% RH and a 12L:12D photoperiod. Ninetyfive percent of the eggs hatched at 20, 25, and 30 °C, respectively. The entire developmental period was 40.3 days at 16 °C and only 9.3 days at 32 °C. The low developmental threshold temperature for development fromegg to adult was 10.1 °C, and the effective accumulated temperature was 197.5 degree–days (DD). Unmated female mites had the longest life span although the oviposition period shortened as temperature increased. Mated female mites produced significantly more eggs than unmated females. At most, a mated female could produce an average of 3.5 eggs per day at 25 °C and a maximum of 60.3 eggs over the entire oviposition period at 20 °C. Unmated females exclusively produce male offspring, indicating that litchi spider mites are arrhenotokous. At 25 °C, the intrinsic rate of increase (r) was 0.1610 day−1, the net reproductive rate (R0) was 19.58 offspring, the finite rate of increase (λ) was 1.1747 day−1, and the mean generation time (T) was 18.50 days. The results obtained in this study provide useful information to aid in the development of phenological models for pest monitoring programs of O. litchii in Taiwan.
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Chen, Wen-Jie,He, De-Shen,Tang, Rui-Xue,Ren, Fang-Hui,Chen, Gang Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.2
Ki-67 has been widely used as an indicator of cell proliferation in gliomas. However, the role of Ki-67 as a prognostic marker is still undefined. Thus, we conducted a meta-analysis of the published literatures in order to clarify the impact of Ki-67 on survival in glioma cases. Eligible studies were identified in PubMed, EMBASE, ISI Web of Science, Cochrane Central Register of Controlled Trials, Science Direct and Wiley Online Library with the last search updated on August 31, 2014. The clinical characteristics, overall survival (OS) and progression-free survival (PFS) together with Ki-67 expression at different time points were extracted. A total of 51 studies, covering 4,307 patients, were included in the current meta-analysis. The results showed that overexpression of Ki-67 can predict poor OS (HR=1.66, 95%CI: 1.53-1.80; Z=11.87; p=0.000) and poor PFS (HR=1.67, 95%CI: 1.47-1.91; Z=7.67; p=0.000) in gliomas. Moreover, subgroup analyses also indicated that high level of Ki-67 expression was related to poor OS and PFS in glioma patients regardless of region, pathology type, cut-off value and statistical method. In conclusion, the current meta-analysis revealed that Ki-67 expression might be a predicative factor for poor prognosis of glioma patients, emphasizing its importance as a predictor.
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Chen, Zhi-Qing,Zhou, You,Huang, Jun-Wen,Chen, Feng,Zheng, Jing,Li, Hao-Liang,Li, Tao,Li, Lang The Korean Society of Pharmacology 2021 The Korean Journal of Physiology & Pharmacology Vol.25 No.2
Coronary microembolization (CME) is associated with cardiomyocyte apoptosis and cardiac dysfunction. Puerarin confers protection against multiple cardiovascular diseases, but its effects and specific mechanisms on CME are not fully known. Hence, our study investigated whether puerarin pretreatment could alleviate cardiomyocyte apoptosis and improve cardiac function following CME. The molecular mechanism associated was also explored. A total of 48 Sprague-Dawley rats were randomly divided into CME, CME + Puerarin (CME + Pue), sham, and sham + Puerarin (sham + Pue) groups (with 12 rats per group). A CME model was established in CME and CME + Pue groups by injecting 42 ㎛ microspheres into the left ventricle of rats. Rats in the CME + Pue and sham + Pue groups were intraperitoneally injected with puerarin at 120 mg/kg daily for 7 days before operation. Cardiac function, myocardial histopathology, and cardiomyocyte apoptosis index were determined via cardiac ultrasound, hematoxylin-eosin (H&E) and hematoxylin-basic fuchsin-picric acid (HBFP) stainings, and TdT-mediated dUTP nick-end labeling (TUNEL) staining, respectively. Western blotting was used to measure protein expression related to the phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt)/glycogen synthase kinase-3β (GSK-3β) pathway. We found that, puerarin significantly ameliorated cardiac dysfunction after CME, attenuated myocardial infarct size, and reduced myocardial apoptotic index. Besides, puerarin inhibited cardiomyocyte apoptosis, as revealed by decreased Bax and cleaved caspase-3, and up-regulated Bcl-2 and PI3K/Akt/GSK-3β pathway related proteins. Collectively, puerarin can inhibit cardiomyocyte apoptosis and thus attenuate myocardial injury caused by CME. Mechanistically, these effects may be achieved through activation of the PI3K/Akt/GSK-3β pathway.
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Wen-Ming Chen,Yi Min Xie,Gabriele Imbalzano,Jianhu Shen,Shanqing Xu,이성재,Peter Vee Sin Lee 한국정밀공학회 2016 International Journal of Precision Engineering and Vol.17 No.6
The development of porous metals to alleviate the effects of stress shielding in bone will help improve the function of metallic biomaterials in orthopaedic applications. A critical step in advancing this technology is to design metallic structures with low rigidity that is comparable with bone tissue, but with good mechanical strength. In this study, porous titanium (Ti) structures with periodic cell topologies were designed to achieve tunable mechanical properties. The versatility of the design scheme was demonstrated by examining lattice designs with different stiffness properties achieved by using the Selective Laser Melting (SLM) technology. The fabricated porous Ti exhibited a low modulus of 1.05 GPa but a high compressive strength of 55 MPa. Large deformation analysis using digital image correlation (DIC) technique indicated uniform strain patterns at micro-trusses, suggesting the overall high quality of the structure with absence of local flaws. A functionally-graded stiffness design was further investigated by varying the diameters of micro-trusses within the structure. A stiffness graded material may be favourable for anatomical site that has strong depthdependent variations, such as in trabecular bone microstructures.
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