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Suteerapongpun, Piyoros,Wattanachai, Tanapan,Janhom, Apirum,Tripuwabhrut, Polbhat,Jotikasthira, Dhirawat Korean Academy of Oral and Maxillofacial Radiology 2018 Imaging Science in Dentistry Vol.48 No.1
Purpose: To perform a comparative analysis of the palatal bone thickness in Thai patients exhibiting class I malocclusion according to whether they exhibited a normal or open vertical skeletal configuration using cone-beam computed tomography (CBCT). Materials and Methods: Thirty CBCT images of Thai orthodontic patients (15-30 years of age) exhibiting class I malocclusion with a normal or open vertical skeletal configuration were selected. Palatal bone thickness was measured in a 3.0-mm grid pattern on both the right and left sides. The palatal bone thickness of the normal-bite and open-bite groups was compared using the independent t-test. The level of significance was established at P<.05. Results: The palatal bone thickness in the normal-bite group ranged from $2.2{\pm}1.0mm$ to $12.6{\pm}4.1mm$. The palatal bone thickness in the open-bite group ranged from $1.9{\pm}1.1mm$ to $13.2{\pm}2.3mm$. The palatal bone thickness was lower at almost all sites in patients with open bite than in those with normal bite. Significant differences were found at almost all anteroposterior sites along the 3 most medial sections (3.0, 6.0, and 9.0 mm lateral to the midsagittal plane)(P<.05). Conclusion: Class I malocclusion with open vertical skeletal configuration may affect palatal bone thickness, so the placement of temporary anchorage devices or miniscrew implants in the palatal area in such patients should be performed with caution.
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Biological Control of Phytophthora palmivora Causing Root Rot of Pomelo Using Chaetomium spp.
Hung, Phung Manh,Wattanachai, Pongnak,Kasem, Soytong,Poaim, Supatta The Korean Society of Mycology 2015 Mycobiology Vol.43 No.1
Phytophthora diseases have become a major impediment in the citrus production in Thailand. In this study, an isolate of Phytophthora denominated as PHY02 was proven to be causal pathogen of root rot of Pomelo (Citrus maxima) in Thailand. The isolate PHY02 was morphologically characterized and identified as Phytophthora palmivora based on molecular analysis of an internal transcribed spacer rDNA sequence. This work also presents in vitro evaluations of the capacities of Chaetomium spp. to control the P. palmivora PHY02. As antagonists, Chaetomium globosum CG05, Chaetomium cupreum CC3003, Chaetomium lucknowense CL01 inhibited 50~61% mycelial growth, degraded mycelia and reduced 92~99% sporangial production of P. palmivora PHY02 in bi-culture test after 30 days. Fungal metabolites from Chaetomium spp. were tested against PHY02. Results showed that, methanol extract of C. globosum CG05 expressed strongest inhibitory effects on mycelial growth and sporangium formation of P. palmivora PHY02 with effective dose ED50 values of $26.5{\mu}g/mL$ and $2.3{\mu}g/mL$, respectively. It is interesting that C. lucknowense is reported for the first time as an effective antagonist against a species of Phytophthora.
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( Phung Manh Hung ),( Pongnak Wattanachai ),( Soytong Kasem ),( Supattra Poeaim ) 한국균학회 2015 Mycobiology Vol.43 No.3
Thailand is one of the largest citrus producers in Southeast Asia. Pathogenic infection by Phytophthora, however, has become one of major impediments to production. This study identified a pathogenic oomycete isolated from rotted roots of pomelo (Citrus maxima) in Thailand as Phytophthora nicotianae by the internal transcribed spacer ribosomal DNA sequence analysis. Then, we examined the in vitro and in vivo effects of Chaetomium globosum, Chaetomium lucknowense, Chaetomium cupreum and their crude extracts as biological control agents in controlling this P. nicotianae strain. Represent as antagonists in biculture test, the tested Chaetomium species inhibited mycelial growth by 50~56% and parasitized the hyphae, resulting in degradation of P. nicotianae mycelia after 30 days. The crude extracts of these Chaetomium species exhibited antifungal activities against mycelial growth of P. nicotianae, with effective doses of 2.6~101.4 μg/mL. Under greenhouse conditions, application of spores and methanol extracts of these Chaetomium species to pomelo seedlings inoculated with P. nicotianae reduced root rot by 66~71% and increased plant weight by 72~85% compared to that in the control. The method of application of antagonistic spores to control the disease was simple and economical, and it may thus be applicable for large-scale, highly effective biological control of this pathogen.
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Suteerapongpun, Piyadanai,Sirabanchongkran, Supassara,Wattanachai, Tanapan,Sriwilas, Patiyut,Jotikasthira, Dhirawat Korean Academy of Oral and Maxillofacial Radiology 2017 Imaging Science in Dentistry Vol.47 No.4
Purpose: The aim of this study was to compare the root surface areas of the maxillary permanent teeth in Thai patients exhibiting anterior normal overbite and in those exhibiting anterior open bite, using cone-beam computed tomography (CBCT). Materials and Methods: CBCT images of maxillary permanent teeth from 15 patients with anterior normal overbite and 18 patients with anterior open bite were selected. Three-dimensional tooth models were constructed using Mimics Research version 17.0. The cementoenamel junction was marked manually. The root surface area was calculated automatically by 3-Matic Research version 9.0. The root surface areas of each tooth type from both types of bite were compared using the independent t-test (P<.05). The intraclass correlation coefficient was used to assess intraobserver reliability. Results: The mean root surface areas of the maxillary central and lateral incisors in individuals with anterior open bite were significantly less than those in those with normal bite. The mean root surface area of the maxillary second premolar in individuals with anterior open bite was significantly greater than in those with normal bite. Conclusion: Anterior open-bite malocclusion might affect the root surface area, so orthodontic force magnitudes should be carefully determined.
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Biological Control of Phytophthora palmivora Causing Root Rot of Pomelo Using Chaetomium spp
( Phung Manh Hung ),( Pongnak Wattanachai ),( Soytong Kasem ),( Supatta Poaim ) 한국균학회 2015 韓國菌學會誌 Vol.43 No.1
Phytophthora diseases have become a major impediment in the citrus production in Thailand. In this study, an isolate of Phytophthora denominated as PHY02 was proven to be causal pathogen of root rot of Pomelo (Citrus maxima) in Thailand. The isolate PHY02 was morphologically characterized and identified as Phytophthora palmivora based on molecular analysis of an internal transcribed spacer rDNA sequence. This work also presents in vitro evaluations of the capacities of Chaetomium spp. to control the P. palmivora PHY02. As antagonists, Chaetomium globosum CG05, Chaetomium cupreum CC3003, Chaetomium lucknowense CL01 inhibited 50~61% mycelial growth, degraded mycelia and reduced 92~99% sporangial production of P. palmivora PHY02 in bi-culture test after 30 days. Fungal metabolites from Chaetomium spp. were tested against PHY02. Results showed that, methanol extract of C. globosum CG05 expressed strongest inhibitory effects on mycelial growth and sporangium formation of P. palmivora PHY02 with effective dose ED50 values of 26.5 μg/mL and 2.3 μg/mL, respectively. It is interesting that C. lucknowense is reported for the first time as an effective antagonist against a species of Phytophthora.
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Tasanapanont, Jintana,Apisariyakul, Janya,Wattanachai, Tanapan,Sriwilas, Patiyut,Midtbo, Marit,Jotikasthira, Dhirawat Korean Academy of Oral and Maxillofacial Radiology 2017 Imaging Science in Dentistry Vol.47 No.2
Purpose: The aim of this study was to compare the use of 3-dimensional (3D) laser scanning and cone-beam computed tomography (CBCT) as methods of root surface measurement. Materials and Methods: Thirty teeth (15 maxillary first premolars and 15 mandibular first premolars) from 8 patients who required extractions for orthodontic treatment were selected. Before extraction, pre-treatment CBCT images of all the patients were recorded. First, a CBCT image was imported into simulation software (Mimics version 15.01; Materialise, Leuven, Belgium) and the root surface area of each tooth was calculated using 3-Matic (version 7.01, Materialise, Leuven, Belgium). After extraction, all the teeth were scanned and the root surface area of each extracted tooth was calculated. The root surface areas calculated using these 2 measurement methods were analyzed using the paired t-test (P<.05). Correlations between the 2 methods were determined by calculating the Pearson correlation coefficient. The intraclass correlation coefficient(ICC) was used to assess intraobserver reliability. Results: The root surface area measurements ($230.11{\pm}41.97mm^2$) obtained using CBCT were slightly greater than those ($229.31{\pm}42.46mm^2$) obtained using 3D laser scanning, but not significantly (P=.425). A high Pearson correlation coefficient was found between the CBCT and the 3D laser scanner measurements. The intraobserver ICC was 1.000 for 3D laser scanning and 0.990 for CBCT. Conclusion: This study presents a novel CBCT approach for measuring the root surface area; this technique can be used for estimating the root surface area of non-extracted teeth.
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Piyoros Suteerapongpun,Tanapan Wattanachai,Apirum Janhom,Polbhat Tripuwabhrut,Dhirawat Jotikasthira 대한영상치의학회 2018 Imaging Science in Dentistry Vol.48 No.1
Purpose: To perform a comparative analysis of the palatal bone thickness in Thai patients exhibiting class I malocclusion according to whether they exhibited a normal or open vertical skeletal configuration using cone-beam computed tomography (CBCT). Materials and Methods: Thirty CBCT images of Thai orthodontic patients (15-30 years of age) exhibiting class I malocclusion with a normal or open vertical skeletal configuration were selected. Palatal bone thickness was measured in a 3.0-mm grid pattern on both the right and left sides. The palatal bone thickness of the normal-bite and open-bite groups was compared using the independent t-test. The level of significance was established at P<.05. Results: The palatal bone thickness in the normal-bite group ranged from 2.2±1.0 mm to 12.6±4.1 mm. The palatal bone thickness in the open-bite group ranged from 1.9±1.1 mm to 13.2±2.3 mm. The palatal bone thickness was lower at almost all sites in patients with open bite than in those with normal bite. Significant differences were found at almost all anteroposterior sites along the 3 most medial sections (3.0, 6.0, and 9.0 mm lateral to the midsagittal plane) (P<.05). Conclusion: Class I malocclusion with open vertical skeletal configuration may affect palatal bone thickness, so the placement of temporary anchorage devices or miniscrew implants in the palatal area in such patients should be performed with caution.
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Optimal Port Placement could Improve the Ergonomic Design of Laparoscope Manipulating Robot
Kovit Khampitak,Suchat Wattanachai,Panisara Kunkitti,Naruepon Kumpa,Sirivit Techajedchadarungsri,Papada Samsong,Tueanjit Khampitak,Kanok Seejorn 제어로봇시스템학회 2010 제어로봇시스템학회 국제학술대회 논문집 Vol.2010 No.10
Laparoscope manipulating robot is useful for maintaining a stable view during a laparoscopic operation. However, a large apparatus can interfere with surgical space, set-up time and repositioning. Furthermore, it consumes a lot of initial and maintenance cost. Ten consecutive robotic assisted laparoscopic pelvic surgeries were performed in order to study the maximum and optimal motion-angle in difference port placements. The maximum horizontal angle(X) for right pelvic view (Mx Rt X) was 30 degree and the maximum horizontal angle(X) for left pelvic view (Mx Lt X) was -25 degree. The median of maximum vertical angle (Y) for upward motion (Mx Up Y) was 44 degree (range 32-55 degree) and the median of maximum vertical angle (Y) for downward motion (Mx Dn Y) was 24 degree. The median optimal angle in operation of right uterine horn was 14 degree (range 3-30 degree) in horizontal plane (Opt Rt X) and 28 degree (range 24-43 degree) in vertical plane (Opt Rt Y). The median optimal angle in operation of left uterine horn was -10 degree (range -4- -25 degree) in horizontal plane (Opt Lt X) and 29 degree (range 24-51 degree) in vertical plane (Opt Lt Y). The median optimal depth (Z) of endoscope position in right uterine horn operation (Opt In Rt Z) was 9.5 cm (range 7.5 -12.5 cm) and left(Opt In Lt Z) was 9.5 cm (range 6-12.5 cm). L, U, +1U ports were selected by an experience gynecologic laparoscopist as the suitable port mimicked to human pelvic surgery. Then we concluded that the endoscope camera needed to move from -30 degree to +30 degree in the left to right direction (X) and from 0 degree to +60 degree in the up/down (Y) direction and, 15 cm for in/out motion (Z) in order to reach the maximum angle for full pelvic operation. From this results, we hope to design an ergonomic robot with less interferes with the surgeon.
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Piyadanai Suteerapongpun,Supassara Sirabanchongkran,Tanapan Wattanachai,Patiyut Sriwilas,Dhirawat Jotikasthira 대한영상치의학회 2017 Imaging Science in Dentistry Vol.47 No.4
Purpose: The aim of this study was to compare the root surface areas of the maxillary permanent teeth in Thai patients exhibiting anterior normal overbite and in those exhibiting anterior open bite, using cone-beam computed tomography (CBCT). Materials and Methods: CBCT images of maxillary permanent teeth from 15 patients with anterior normal overbite and 18 patients with anterior open bite were selected. Three-dimensional tooth models were constructed using Mimics Research version 17.0. The cementoenamel junction was marked manually. The root surface area was calculated automatically by 3-Matic Research version 9.0. The root surface areas of each tooth type from both types of bite were compared using the independent t-test (P<.05). The intraclass correlation coefficient was used to assess intraobserver reliability. Results: The mean root surface areas of the maxillary central and lateral incisors in individuals with anterior open bite were significantly less than those in those with normal bite. The mean root surface area of the maxillary second premolar in individuals with anterior open bite was significantly greater than in those with normal bite. Conclusion: Anterior open-bite malocclusion might affect the root surface area, so orthodontic force magnitudes should be carefully determined.
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