분만 전·후 사료급여 형태가 젖소의 생산성에 미치는 영향

기광석,김현섭,이왕식,이현준,김상범,정하연,은정식,김용국 충남대학교 농업과학연구소 2007 농업과학연구 Vol.34 No.2

This experiment was carried out to protect drops of feed intake should be plural prepartum and postpartum to reduce metabolic diseases after calving to know how feeding systems, which is divided as a low quility, a high quility and total mixed rations(TMR), affects on postpartum productivity of Holistein cows. Three diets (low or high quality forage separately fed with concentrate and TMR containing high quality roughage) were fed to 21 cows from 3 weeks prepartum to 8 weeks postpartum to examine their effects on the productivity of cows. DM intakes was noticed significantly higher with TMR (17.11kg/day) than low- quality (13.48 kg/day) and high-quality forage (13.10kg/day). TDN and CP intakes were also higher with TMR compared to other experimental diets. Mean daily milk yield was non-significant among the cows fed different diets. Blood non-esterified fatty acids(NEFA) content was higher in cows fed low-quality or high-quality forage separately with concentrate compared with those fed TMR. The results concluded that TMR feeding to transitional cows is better than feeding the low or high quality forage separately for their health and productivity. 본 시험은 분만 전 3주부터 분만 후 3주까지 전환기라고 지칭되는 시기의 젖소들에 대한 대사적 변화를 최소화하기 위한 방법으로 사료 섭취량 저하를 방지하고 분만 후 대사성 질병의 발생을 줄이기 위한 목적으로 수행되어졌다. 대사성 질병에 관여하는 주요 요인으로는 건물섭취량, 혈중 glucose 함량, NEFA 함량, 반추위 환경, 면역 등인데, 분만 전·후 Holstein 젖소에 사료급여 형태를 조·농분리(저질 조사료와 양질 조사료 급여구) 및 TMR로 다르게 하였을 때 사료섭취량 변화와 혈중 NEFA 및 glucose 함량 변화를 측정하고 분만 후 젖소의 생산성에 미치는 영향을 구명하기 위하여 수행하였다. 처리별 사료건물 섭취량은 저질 조사료 조·농 분리, 양질조사료 조·농 분리, TMR 급여구에서 각각 13.48, 13.10, 17.11kg으로 TMR 급여구에서 유의성 있게 높았으며(P〈0.05), TDN 섭취량은 처리구별 각각 7.35, 7.19, 5.66kg으로 처리간에 차이가 없었다(P〉0.05). 신체충실지수(BCS)는 저질조사료구에서는 3.03이였으며, 양질 조사료구와 TMR 급여구에서는 각각 3.19, 3.16이었다. 분만후 1주부터 5주까지 측정한 평균 산유량은 저질조사료 조·농 분리, 양질 조사료 조·농 분리, TMR 급여구에서 각각 34.1, 35.1, 35.6kg 였으며, 분만 후 4∼5주에 최고 비유기에 달했다. 처리별 평균 유지율은 저질 조사료 조·농 분리, 양질 조사료 조·농 분리, TMR 급여구에서 각각 3.74, 3.68, 3.95%였다. 혈청내 NEFA 함량은 저질 조사료 조·농 분리, 양질 조사료 조·농 분리, TMR 급여구에서 각각 744.4, 381.8,266.7μEq/L로 처리간에 큰 차이를 보여 양질 조사료 급여와 TMR 급여시 혈중 NEFA 함량이 저질 조사료 급여에 이해 유의성 있게 낮았다(P〈0.05). 혈청내 glucose함량은 저질조사료 및 양질 조사료 조·농 분리, TMR 급여구에서 각각 39.3, 56.3, 62.4mg/dl로 양질 조사료 급여와 TMR 급여시 혈중 glucose 함량이 저질 조사료급여에 비해 유의성 있게 높았다(P〈0.05). 이상의 결과들을 종합해 볼 때, 분만 전 3주부터 분만 후 3주까지의 전환기 동안 사료섭취량 증가와 대사성 질병의 지표로서 혈중 NEFA 및glucose 함량 변화를 살펴 본 결과 분만 전 3주부터는 양질의 조사료나 TMR 형태로 급여하는 것이 사료섭취량 증가와 대사성 질병의 발생을 감소시킬 수 있을 것으로 사료되었다.

Inhibitory effects of melittin and phospholipase A2 of bee venom against rabies virus

Ha-Hyun Kim(Ha-Hyun Kim),Dong-Kun Yang(Dong-Kun Yang),Ja-Young Wang(Ja-Young Wang),In-Soo Cho(In-Soo Cho) 한국예방수의학회 2017 한국예방수의학회 학술대회자료집 Vol.2017 No.-

The Links between Science, Technology and Urbanization on the Road towards Chinese Exceptionalism

Wang, Bo,Tao, Jill L.,Lee, Jong-Youl,Ha, Hyun-Sang 부산대학교 중국연구소 2018 Journal of China Studies Vol.21 No.4

Structural insight into the carboxylesterase BioH from <i>Klebsiella pneumoniae</i>

Wang, Lulu,Chen, Yuanyuan,Shang, Fei,Liu, Wei,Lan, Jing,Gao, Peng,Ha, Nam-Chul,Nam, Ki Hyun,Dong, Yuesheng,Quan, Chunshan,Xu, Yongbin Elsevier 2019 Biochemical and biophysical research communication Vol.520 No.3

<P><B>Abstract</B></P> <P>The BioH carboxylesterase which is a typical α/β-hydrolase enzyme involved in biotin synthetic pathway in most bacteria. BioH acts as a gatekeeper and blocks the further elongation of its substrate. In the pathogen <I>Klebsiella pneumoniae</I>, BioH plays a critical role in the biosynthesis of biotin. To better understand the molecular function of BioH, we determined the crystal structure of BioH from <I>K. pneumoniae</I> at 2.26 Å resolution using X-ray crystallography. The structure of KpBioH consists of an α-β-α sandwich domain and a cap domain. B-factor analysis revealed that the α-β-α sandwich domain is a rigid structure, while the loops in the cap domain shows the structural flexibility. The active site of KpBioH contains the catalytic triad (Ser82-Asp207-His235) on the interface of the α-β-α sandwich domain, which is surrounded by the cap domain. Size exclusion chromatography shows that KpBioH prefers the monomeric state in solution, whereas two-fold symmetric dimeric formation of KpBioH was observed in the asymmetric unit, the conserved Cys31-based disulfide bonds can maintain the irreversible dimeric formation of KpBioH. Our study provides important structural insight for understanding the molecular mechanisms of KpBioH and its homologous proteins.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Crystal structure of <I>K. pneumoniae</I>BioH consists of an α-β-αsandwish domain and acapsubdomain. </LI> <LI> The active site of KpBioH contains the catalytic triad (Ser82-Asp207-His235). </LI> <LI> The Arg138, Arg142, Arg155, and Arg159 of KpBioH may play an important role in the binding of the substrates like ACP. </LI> <LI> The Cys31-based disulfide bonds can maintain the irreversible dimeric formation of KpBioH. </LI> </UL> </P>

Non-structural 5A protein of hepatitis C virus induces a range of liver pathology in transgenic mice

Wang, Ai-Guo,Lee, Dong-Seok,Moon, Hyung-Bae,Kim, Jin-Man,Cho, Kyung-Hyun,Choi, Soo-Ho,Ha, Hye-Lin,Han, Ying-Hao,Kim, Dae-Ghon,Hwang, Soon B.,Yu, Dae-Yeul John Wiley Sons, Ltd. 2009 The Journal of pathology Vol.219 No.2

<P>Hepatitis C virus (HCV) is a major cause of chronic hepatitis, liver cirrhosis and hepatocellular carcinoma (HCC). However, the mechanism of HCV pathogenesis is not well understood. Our previous in vitro studies suggested that non-structural 5A (NS5A) protein may play an important role in liver pathogenesis. To elucidate the mechanism of HCV-induced liver pathogenesis, we investigated the histopathological changes of liver in transgenic mice harbouring the NS5A gene. We generated transgenic mice harbouring HCV NS5A gene under the control of hepatitis B virus (HBV) enhancer. Pathological changes were analysed by immunohistochemical staining and western blot analysis. Lipid composition and reactive oxygen species (ROS) production in NS5A transgenic mice were analysed. HCV NS5A transgenic mice developed extraordinary steatosis over 6 months old and induced HCC in some mice. NS5A was co-localized with apolipoprotein A-I in fatty hepatocytes. In addition, the extraordinarily high levels of ROS, NF-κB and STAT3 were detected in hepatocytes of NS5A transgenic mice. These data suggest that NS5A, independent of other HCV viral proteins, may play an important role in the development of hepatic pathologies, including steatosis and hepatoceullular carcinoma in transgenic mice. Copyright © 2009 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.</P>

Hypoxic condition enhances chondrogenesis in synovium-derived mesenchymal stem cells

Hyun Cheol Bae,Hee Jung Park,Sun Young Wang,Ha Ru Yang,Myung Chul Lee,한혁수 한국생체재료학회 2018 생체재료학회지 Vol.22 No.4

Background: The chondrogenic differentiation of mesenchymal stem cells (MSCs) is regulated by many factors, including oxygen tensions, growth factors, and cytokines. Evidences have suggested that low oxygen tension seems to be an important regulatory factor in the proliferation and chondrogenic differentiation in various MSCs. Recent studies report that synovium-derived mesenchymal stem cells (SDSCs) are a potential source of stem cells for the repair of articular cartilage defects. But, the effect of low oxygen tension on the proliferation and chondrogenic differentiation in SDSCs has not characterized. In this study, we investigated the effects of hypoxia on proliferation and chondrogenesis in SDSCs. Method: SDSCs were isolated from patients with osteoarthritis at total knee replacement. To determine the effect of oxygen tension on proliferation and colony-forming characteristics of SDSCs, A colony-forming unit (CFU) assay and cell counting-based proliferation assay were performed under normoxic (21% oxygen) or hypoxic (5% oxygen). For in vitro chondrogenic differentiation, SDSCs were concentrated to form pellets and subjected to conditions appropriate for chondrogenic differentiation under normoxia and hypoxia, followed by the analysis for the expression of genes and proteins of chondrogenesis. qRT-PCR, histological assay, and glycosoaminoglycan assays were determined to assess chondrogenesis. Results: Low oxygen condition significantly increased proliferation and colony-forming characteristics of SDSCs compared to that of SDSCs under normoxic culture. Similar pellet size and weight were found for chondrogensis period under hypoxia and normoxia condition. The mRNA expression of types II collagen, aggrecan, and the transcription factor SOX9 was increased under hypoxia condition. Histological sections stained with Safranin-O demonstrated that hypoxic conditions had increased proteoglycan synthesis. Immunohistochemistry for types II collagen demonstrated that hypoxic culture of SDSCs increased type II collagen expression. In addition, GAG deposition was significantly higher in hypoxia compared with normoxia at 21 days of differentiation. Conclusion: These findings show that hypoxia condition has an important role in regulating the synthesis ECM matrix by SDSCs as they undergo chondrogenesis. This has important implications for cartilage tissue engineering applications of SDSCs.

Microbiological Analysis of Rice Cake Processing in Korea

WANG, JUN,PARK, JOONG-HYUN,CHOI, NA-JUNG,HA, SANG-DO,OH, DEOG-HWAN International Association for Food Protection 2016 Journal of food protection Vol.79 No.1

<B>ABSTRACT</B><P>This study was conducted to evaluate the microbial contamination in rice cake materials and products during processing and in the operation environment in nonhazard analysis [and] critical control point factories. Furthermore, the environmental health of the processing facilities and the bacterial and fungal contamination on the workers' hands were investigated. Pour plate methods were used for enumeration of aerobic plate count (APC), yeast and molds (YM), Bacillus cereus, Staphylococcus aureus, and Clostridium perfringens, whereas Petrifilm count plates were used for enumeration of coliforms and Escherichia coli. The respective microbial levels of APC, coliforms, YM, and B. cereus were in the range of 2.6 to 4.7, 1.0 to 3.8, not detected (ND) to 2.9, and ND to 2.8 log CFU/g in the raw materials and in the range of 2.3 to 6.2, ND to 3.6, ND to 2.7, and ND to 3.7 log CFU/g during processing of the rice cake products. During the processing of rice cakes, APC, coliforms, YM, and B. cereus increased during soaking and smashing treatments and decreased after steaming treatment. E. coli, S. aureus, and C. perfringens were not detected in any of the raw materials and operating areas or during processing. B. cereus was detected on the operators' hands at microbial contamination levels of 1.9 ± 0.19 to 2.0 ± 0.19 log CFU/g. The results showed that B. cereus in the end product is presumably the main concern for rice cakes. In addition, the high contamination level of B. cereus during manufacturing processes, including soaking, smashing, and molding, and the absence of B. cereus from the air sampling plates indicated that the contaminated equipment showed the potential risk to cause cross-contamination.</P>

Effect of moderate exercise training and low-fat diet on peritoneal macrophage immunocompetence in high-fat diet-induced obese mice model

( Wang Lok Lee ),( Jun Hyun Jeong ),( Hee Geun Park ),( Youn Gran Lee ),( Soon Mi Kwon ),( Ha Kyoung Jang ),( Jong Kui Jun ),( Myung Hwa Kim ),( Sang Ki Lee ),( Kwang Mu Lee ) 한국운동영양학회 2012 Physical Activity and Nutrition (Phys Act Nutr) Vol.16 No.3

This study investigated the effect of moderate exercise training on macrophage immunocompetence in high fat diet-induced obese mice. To determine the differential effects of exercise training and low fat diet on macrophage, C57BL/6 male mice (4 week old, n=32) were fed high-fat diet for 5 weeks to induce obese. The high fat-induced obese mice were then divided into four groups and differently treated by high/low fat diet and exercise training for a further 8 weeks; HHC (13 weeks high-fat diet), HHE (13 weeks high-fat diet with 8 weeks Exercise training) and HLC (5 weeks high-fat diet and 8 weeks low-fat diet), HLE (5 weeks high-fat diet and 8 weeks low-fat diet with exercise training). The exercise groups were performed on a motorized treadmill, running for 30-60 min/day at 10-22 m/min, 0% grade for 8wks. Body weight was significantly decreased for those with a low-fat diet and exercise training (HLE). Macrophage phagocytosis was significantly increased by low-fat diet and exercise training (HLE). The percentage of stimulation of NO release induced by LPS was higher in HLE than in other groups. The percentage of stimulation of IL-1β release induced by LPS was higher in HHE and HLE than in other groups. The percentage of stimulation of IFN-γ release induced by LPS was higher in HLE than in other groups. These findings suggest that moderate exercise and low-fat diet have beneficial effects on macrophage immunocompetence in high fat diet-induced obese mice.

구름버섯 알칼리 추출물에서 분리한 항응고성 다당류

이현순(Hyun-Sun Lee),권미향(Mee-Hyang Kweon),임왕진(Wang-Jin Lim),성하진(Ha-Chin Sung),양한철(Han-Chul Yang) 한국식품과학회 1997 한국식품과학회지 Vol.29 No.2

중엽증후군을 유발한 기관지 지방종

김현준 ( Hyun Jun Kim ),왕준호 ( Jun Ho Wang ),인행환 ( Haing Hwan In ),송기호 ( Ki Ho Song ),송종오 ( Jong Oh Song ),송광선 ( Kwang Seon Song ),용석중 ( Suk Jung Yong ),신계철 ( Kye Chul Shin ),홍순원 ( Soon Won Hong ) 대한결핵 및 호흡기학회 1997 Tuberculosis and Respiratory Diseases Vol.44 No.1