백서 뇌에서 전기경련 충격에 의해 발현이 조절되는 새로운 유전자

정선주,박정은,강웅구,구영진,박주배,김용식 大韓神經精神醫學會 2000 신경정신의학 Vol.39 No.5

연구목적: 이 연구는 백서 뇌에서 전기경련 충격(Electroconvulsive shock, ECS)에 의해 발현이 조절되는 새로운 유전자를 검색하고, 발달단게 및 뇌 조직에 따른 발현양상을 관찰하기 위하여 시행되었다. 방법: 생후 7일, 21일된 어린 웅성 백서에 전기경련 충격을 가한 후 mRNS differential display-PCR(DD-PCR) 기법을 시행하여 발현의 차이를 나타내는 유전자를 검색한 후 서열분석을 실시하였다. 생후 7일 및 21일된 백서의 뇌와 성년 백서의 대뇌피질, 해마를 이용한 Northern blot 분석을 시행하여 검색된 유전자의 ECS 처치 후 시간경과에 따른 유전자 발현양상을 관찰하였다. 결과: ECS 처치군과 위충격군간에 발현의 차이를 나타낸 유전자 4개를 검색할 수 있었으며, 이중 ECS에 의해 발현이 증가되는 1개의 유전자를 선정하여 서열분석을 실시한 결과 EST 서열만이 보고된 새로운 유전자임이 밝혀졌다. 이 유전자는 생후 7일된 백서의 뇌에서는 ECS 처치 후 180분에 발현양의 증가가 최고치에 도달하였으나, 생후 21일된 백서의 뇌와 성년백서의 대뇌피질 및 해마에서는 360분 경과시까지 지속적으로 발현 양이 증가하였다. 결론: 이같은 결과는 ECS에 의해 생체 내에서 유전자 발현의 조절이 이루어짐을 시사하며, 향후 이러한 유전자의 세포 내 기능에 관한 연구를 시행함으로써, ECS에 의한 생체의 반응과 치료기전에 대한 이해를 넓힐 수 있을 것으로 기대된다. 중심단어:전기경련 충격·DD-PCR·유전자·백서 뇌. Objectives: This study was performed to identify genes regulated by electroconvulsive shock (ECS) and to observe the pattern of expression of genes according to different developmental stages and brain regions. Method: ECS(130V, 0.5 sec) was given to male Sprague-Dawley rats with age of postnatal day 7 and 21(P7, P21 respectively). After screening genes regulated by ECS with mRNA diffe-rential display-PCR(DD-PCR), we selected one clone among them and observed the induction of this gene after ECS by time-dependent Northern blot analysis of rat brain of P7, P21 and adult rat cortex and hippocampus. Results: By DD-PCR method, we have identified four clones whose expression was regulated by ECS. Among them, one(CP 10-2) was proved to be a new gene by sequencing and BLAST search. Its expression was increased after ECS in P7, P21, and adult rat brain. The expression of CP 10-2 reached peak level at 180 minutes after ECS in P7 rat brain, but was further increased until 360 minutes after ECS in P21 and adult rat brain. Conclusion: In this study, a new gene was identified in rat brain which showed up-regulated expression in response to ECS. Cloning and characterization of this new gene would be helpful to elucidate the effect of ECS in rat brain. KEY WORDS:Electroconvulsive shock·Differential display-PCR·Gene·Rat brain.

TRAM Flap Reconstruction

Ung Sik Jin 대한외과학회 2015 대한외과학회 학술대회 초록집 Vol.2015 No.11

Hybrid Lymphovenous Anastomosis Surgery Guided by Intraoperative Mesenteric Intranodal Lymphangiography for Refractory Nontraumatic Chylous Ascites: A Case Report

Soo Jin Woo,Saebeom Hur,Hee Seung Kim,Hak Chang,Ji-Young Kim,Soo Jin Park,Ung Sik Jin Korean Society of Plastic and Reconstructive Surge 2024 Archives of Plastic Surgery Vol.51 No.1

Refractory chylous ascites can cause significant nutritional and immunologic morbidity, but no clear treatment has been established. This article introduces a case of a 22-year-old female patient with an underlying lymphatic anomaly who presented with refractory chylous ascites after laparoscopic adnexectomy for ovarian teratoma which aggravated after thoracic duct embolization. Ascites (>3,000 mL/d) had to be drained via a percutaneous catheter to relieve abdominal distention and consequent dyspnea, leading to significant cachexia and weight loss. Two sessions of hybrid lymphovenous anastomosis (LVA) surgery with intraoperative mesenteric lymphangiography guidance were performed to decompress the lymphatics. The first LVA was done between inferior mesenteric vein and left para-aortic enlarged lymphatics in a side-to-side manner. The daily drainage of chylous ascites significantly decreased to 130 mL/day immediately following surgery but increased 6 days later. An additional LVA was performed between right ovarian vein and enlarged lymphatics in aortocaval area in side-to-side and end-to-side manner. The chylous ascites resolved subsequently without any complications, and the patient was discharged after 2 weeks. The patient regained weight without ascites recurrence after 22 months of follow-up. This case shares a successful experience of treating refractory chylous ascites with lymphatic anomaly through LVA, reversing the patient's life-threatening weight loss. LVA was applied with a multidisciplinary approach using intraoperative mesenteric lipiodol, and results showed the possibility of expanding its use to challenging problems in the intraperitoneal cavity.

Improvement of Shoulder Motion in Two-Stage Dual-Plane Implant-Based Breast Reconstruction followed by Radiation Therapy through Delayed Prepectoral Conversion

Jin Sol Park,Ung Sik Jin Korean Society of Plastic and Reconstructive Surge 2024 Archives of Plastic Surgery Vol.51 No.1

Background Although prepectoral implant-based breast reconstruction has recently gained popularity, dual-plane reconstruction is still a better option for patients with poor-quality mastectomy skin flaps. However, shoulder morbidity is aggravated by subpectoral reconstruction, especially in irradiated patients. This study aimed to demonstrate shoulder exercise improvement in subpectoral reconstruction by delayed prepectoral conversion with an acellular dermal matrix (ADM) inlay graft technique at the time of expander-to-implant exchange after irradiation. Methods Patients with breast cancer treated for expander-to-implant exchange after subpectoral expander insertion and subsequent radiotherapy between January 2021 and June 2022 were enrolled. An ADM inlay graft was inserted between the pectoralis major muscle and the previously inserted ADM. The ADM was sutured partially overlapping the pectoralis muscle from the medial side with the transition part, to the muscle border at the lateral side. Perioperative shoulder joint active range-of-motion (ROM) for forward flexion, abduction, and external rotation was also evaluated. Results A total of 35 patients were enrolled in the study. Active shoulder ROM significantly improved from 163 degrees preoperatively to 176 degrees postoperatively in forward flexion, 153 to 175 degrees in abduction, and 69 to 84 degrees in external rotation. There was no difference in patient satisfaction regarding the final outcome between the conventional prepectoral reconstruction group and the study group. Conclusion Shoulder exercises in irradiated patients who underwent subpectoral reconstruction were improved by delayed prepectoral conversion using an ADM inlay graft. It is recommended that subpectoral reconstruction not be ruled out due to concerns regarding muscle contracture and shoulder morbidity in radiation-planned patients with poor mastectomy skin flaps.

Identification of Zygotic and Nucellar Mandarin Seedlings Using Randomly Amplified Polymorphic DNA

Jin-Ung Yun,Hee Beom Yang,Yong-Hwan Jung,Su-Hyun Yun,Kwang Sik Kim,Chan-Shick Kim,Kwan Jeong Song 한국원예학회 2007 Horticulture, Environment, and Biotechnology Vol.48 No.3

The study aimed at evaluating an efficiency of cross breeding in ‘Miyagawa Wase’ and ‘Okitsu Wase’ satsuma mandarin and ‘Shiranuhi’ mandarin through investigating the seed and seedlings formation, the frequency and position of zygotic seedlings, and the influence of pollen parents using randomly amplified polymorphic DNA (RAPD). Seedlings were in vitro cultured with seeds obtained from four crosses of ‘Miyagawa Wase’ (Citrus unshiu) × ‘Ponkan’ (C reticulata), ‘Okitsu Wase’ (C unshiu) × ‘Swingle Citrumelo’ (C. paradisi × Poncirus trifoliate), ‘Shiranuhi’ [(C unshiu × C. sinensis) × C. reticulata] × ‘Ponkan’ (C. reticulata), and ‘Shiranuhi’ [(C. unshiu × C. sinensis) × C. reticulata] × ‘Swingle Citrumelo’ (C. paradisi × P. trifoliate). RAPD analysis was conducted with five primers selected from screening 77 primers based on DNA amplification of parent plants. Seed formation including total seeds and full sized seeds per fruit between ‘Miyagawa Wase’ and ‘Okitsu Wase’ was not different statistically. However, seed formation of ‘Shiranuhi’ mandarin was significantly affected by pollen genotype. Average seedlings number per seed was not different effectively. The frequency and positioning patterns of zygotic seedling were different in each cultivar. The results suggest that seed formation, polyembryony, and frequency and positioning of zygotic seedling in some citrus having polyembryony might be affected by seed and pollen parents.

성인형호흡장애증후군을 동반한 속립성결핵 1 예 보고

김진호,김한일,강종식,박종하,이준상,정철호,전건웅 대한내과학회 1986 대한내과학회지 Vol.31 No.4

Miliary tuberculosis is an unusual cause of adult respiratory distress syndrome. In this case, We described one patient with miliary tuberculosis who developed adult respiratory distress syndrome. The chest radiography on admission was normal. During hospitalization repeated chest X-rays revealed a miliary pattern followed by diffuse pulmonary infiltration, and severe respiratory distress developed. The disgnosis of tuberculosis was suspected early, appropriate therapy was initiated, and he survived. The experience with this case serves to emphasize that miliary tuberculosis should be considered in patients presenting with adult respiratory di8tress syndrome of unknown causes.

Pathophysiological Regulation of Vascular Smooth Muscle Cells by Prostaglandin F<SUB>2α</SUB>-dependent Activation of Phospholipase C-β3

Ki Ung Kang(강기웅),Jun Young Oh(오준영),Yun Ha Lee(이윤한),Hye Sun Lee(이혜선),Seo Yeon Jin(진서연),Sun Sik Bae(배순식) 한국생명과학회 2018 생명과학회지 Vol.28 No.12

죽상동맥경화는 대동맥의 만성염증에 의해 주로 발병되는 폐쇄동맥질환이다. 혈관평활근세포의 증식 및 이동은 죽상동맥경화 발병의 주된 병리적 반응이다. 본 연구에서는 죽상동맥경화 발병기전을 유도하는 표적 염증반응물질의 탐색 및 이들에 의한 신호전달 기전을 연구하였다. 혈관평활근세포의 증식 및 이동은 prostaglandin F2α (PGF2α)에 의해 의미 있게 증가하였으나 tumor necrosis factor α (TNFα)에 의해서는 증가하지 않았다. Prostacyclin I₂ (PGI₂)는 혈관평활근세포의 증식은 촉진시켰으나 이동은 오히려 억제하였다. prostaglandin D₂ (PGD2) 및 prostaglandin E₂ (PGE₂)는 혈관평활근세포의 증식을 촉진시켰으나 이동에는 영향을 미치지 않았다. PGF2α는 용량 의존적으로 혈관평활근세포의 증식 및 이동을 촉진시켰고 EC50는 약 0.1 μM로 관찰되었다. 혈관평활근세포에서 phospholipase C-β3 (PLC-β3) 아형의 발현은 매우 높았으나 PLC-β1, PLC-β2, 및 PLC-β4의 발현은 관찰되지 않았다. U73122 처리를 통해 PLC의 활성을 억제하면 PGF2α에 의한 혈관평활근세포의 이동이 억제되었다. 또한 PLC-β3의 발현을 억제하면 PGF2α에 의한 혈관평활근세포의 증식 및 이동이 억제되었다. 이러한 결과들을 바탕으로 PGF2α 는 혈관평활근세포의 증식 및 이동에 중요한 역할을 수행하고, 여기에는 PLC-β3가 필수적인 역할을 담당하고 있음을 제안한다. Atherosclerosis is an obstructive vessel disease mainly caused by chronic arterial inflammation to which the proliferation and migration of vascular smooth muscle cells (VSMCs) is the main pathological response. In the present study, the primary responsible inflammatory cytokine and its signaling pathway was investigated. The proliferation and migration of VSMCs was significantly enhanced by the prostaglandin F2α (PGF2α), while neither was affected by tumor necrosis factor α. Prostacyclin I₂ was seen to enhance the proliferation of VSMCs while simultaneously suppressing their migration. Both prostaglandin D₂ and prostaglandin E₂ significantly enhanced the migration of VSMCs, however, proliferation was not affected by either of them. The proliferation and migration of VSMCs stimulated by PGF2α progressed in a dose-dependent manner; the EC50 value of both proliferation and migration was 0.1 μM. VSMCs highly expressed the phospholipase isoform C-β3 (PLC-β3) while others such as PLC-β1, PLC-β2, and PLC-β4 were not expressed. Inhibition of the PLCs by U73122 completely blocked the PGF2α-induced migration of VSMCs, and, in addition, silencing PLC-β3 significantly diminished the PGF2α-induced proliferation and migration of VSMCs. Given these results, we suggest that PGF2α plays a crucial role in the proliferation and migration of VSMCs, and activation of PLC-β3 could be involved in their PGF2α-dependent migration.

Allelic Variation of Rht-1, Vrn-1 and Ppd-1 in Korean Wheats and Its Effect on Agronomic Traits

( Eun Jin Cho ),( Chon Sik Kang ),( Ji Ung Jung ),( Young Mi Yoon ),( Chul Soo Park ) 한국육종학회 2015 Plant Breeding and Biotechnology Vol.3 No.2

The allelic variations at the Rht-1, Vrn-1 and Ppd-1 of 410 Korean wheat cultivars, including 111 Korean experimental lines, 238 Korean landraces and 61 North Korean collections, were investigated to provide the information of plant height and heading date and to elucidate the relationship between those traits and allelic variation of these genes because earliness is major consideration in Korean wheat production. All Korean wheats displayed vrn-A1 and Ppd-A1b alleles, while Rht-B1a, Rht-D1a, vrn-B1, Vrn-D1, Ppd-B1b and Ppd-D1a alleles were also predominantly found. Most Korean wheats carried both Rht-B1a and Rht-D1a alleles, both vrn-B1 and Vrn-D1 alleles, or both Ppd-B1b and Ppd-D1a alleles. The Rht-B1a, vrn-D1, Ppd-B1b and Ppd-D1b alleles were found to exhibit longer culm and spike length than their counterpart alleles. The Rht-B1a allele also showed longer spike length than Rht-B1b. Vrn-B1b and vrn-D1 alleles exhibited longer days to heading date than their counterpart alleles at the Vrn-B1 and Vrn-D1 loci. Lines carrying both Rht-B1b and Rht-D1b alleles displayed shorter culm and longer spike length and days to heading date than any other combination of alleles at the Rht-B1 and Rht-D1 loci. In contrast, lines carrying both Ppd-B1b and Ppd-D1b alleles exhibited longer culm and spike length than any other combination of alleles at the Ppd-B1 and Ppd-D1 loci.

Protein Modifications in Human Diseases(Proteomics) : Characterization of the substantia nigra in mouse: proteomic approach to parkinson`s disease

( Su Jin Lee ),( Sun Mi Yun ),( Hyun Tae Kim ),( Ung Kyu Choi ),( Nam Sik Eum ),( Song Cui ),( Gui Hyang Choi ),( Tae Cheon Kang ),( Soo Young Choi ),( Oh Shin Kwon ) 한국생화학분자생물학회 (구 한국생화학회) 2008 생화학분자생물학회 춘계학술발표논문집 Vol.2008 No.-

Determination of carotenoid components in carrot cultivars from Korea

Chae-Jin Lim,Jeong-Lim Ha,Yong-ung Kim,Hyub Lee,Yong Wook Shin,Hyun Sik Chun,Cheol Ho Lee,Jin Woo Kim,Shin Woo Lee,Mi-Jeong Ahn 한국작물학회 2008 한국작물학회 학술발표대회 논문집 Vol.2008 No.10

Carrots (Daucus carota L.) are consumed as an important dietary source of b-carotene (provitamin A), a-carotene and lutein. An HPLC method was applied to determine the content of the carotenoid composition in carrot cultivars from Korea. Analyses were carried out with five carrot cultivars (Socheon-5-chon, Hongsim-5-chon, Myeongju-5-chon, Seonhongbom-5-chon and Betarich) sown at April, 2007 and six cultivars (Yeoreum-5-chon, Hanyeoreum-5-chon, Sinheukjeon-5-chon, Bibariheukjeon, Manina and Betarich) sown at August of the same year. The former and the latter carrots were harvested after 110 and 96 days from seeding, respectively, and the carotenoids were extracted with acetone after freeze-drying. The amount of a-carotenewas similar to that of b-carotene for the carrot cultivars sown at spring, while the content was about a half of b-carotene content for the latter cultivars. In addition, the average content of lutein in the former cultivars was nine times higher than that in the latter cultivars. Among the spring cultivation types, Socheon-5-chon and Myeongju-5-chon showed higher amount of a-carotene and b-carotene, while the higher amount was determined in Yeoreum-5-chon and Sinheukjeon-5-chon among the autumn cultivation types. This difference between spring cultivation types and autumn cultivation types was also shown in commercial two types of carrots purchased from local market.