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오덕철,김선균,주왕기,조동현,김우호,윤경민 江原大學校附設體力硏究所 1976 江原大學校附設體育科學硏究所論文集 Vol.- No.1
The water quality of the Hong-Cheon river was investigated during the winter season. The rate of contamination of the low tide was a little more than the high tide. But all values of the analyzed-items were similar to those in the clear water.
Kim Gyu Ri,Kim Eun-Young,Kim Si Hyun,Lee Hae Kyung,Lee Jaehyeon,Shin Jong Hee,Kim Young Ree,Song Sae Am,Jeong Joseph,Uh Young,Kim Yu Kyung,Yong Dongeun,Kim Hyun Soo,Kim Sunjoo,Kim Young Ah,Shin Kyeong 대한진단검사의학회 2023 Annals of Laboratory Medicine Vol.43 No.1
Background: Streptococcus pneumoniae is a serious pathogen causing various infections in humans. We evaluated the serotype distribution and antimicrobial resistance of S. pneumoniae causing invasive pneumococcal disease (IPD) after introduction of pneumococcal conjugate vaccine (PCV)13 in Korea and investigated the epidemiological characteristics of multidrug-resistant (MDR) isolates. Methods: S. pneumoniae isolates causing IPD were collected from 16 hospitals in Korea between 2017 and 2019. Serotyping was performed using modified sequential multiplex PCR and the Quellung reaction. Antimicrobial susceptibility tests were performed using the broth microdilution method. Multilocus sequence typing was performed on MDR isolates for epidemiological investigations. Results: Among the 411 S. pneumoniae isolates analyzed, the most prevalent serotype was 3 (12.2%), followed by 10A (9.5%), 34 (7.3%), 19A (6.8%), 23A (6.3%), 22F (6.1%), 35B (5.8%), 11A (5.1%), and others (40.9%). The coverage rates of PCV7, PCV10, PCV13, and pneumococcal polysaccharide vaccine (PPSV)23 were 7.8%, 7.8%, 28.7%, and 59.4%, respectively. Resistance rates to penicillin, ceftriaxone, erythromycin, and levofloxacin were 13.1%, 9.2%, 80.3%, and 4.1%, respectively. MDR isolates accounted for 23.4% of all isolates. Serotypes 23A, 11A, 19A, and 15B accounted for the highest proportions of total isolates at 18.8%, 16.7%, 14.6%, and 8.3%, respectively. Sequence type (ST)166 (43.8%) and ST320 (12.5%) were common among MDR isolates. Conclusions: Non-PCV13 serotypes are increasing among invasive S. pneumoniae strains causing IPD. Differences in antimicrobial resistance were found according to the specific serotype. Continuous monitoring of serotypes and antimicrobial resistance is necessary for the appropriate management of S. pneumoniae infections.
Physicochemical Properties of Heme Iron Products in the Korean Market
Uh-Hyun Kim,Young-Mi Shin,Mie-Jae Im,Seon-Yong Kim 한국식품영양과학회 2006 Journal of medicinal food Vol.9 No.2
Since absorption efficacy of heme iron (HI) is critically dependent on its solubility in aqueous solution, we in-vestigated the physicochemical properties of two HI products available in the Korean market. The two HI products did notdiffer in ingredients and color. However, HI polypeptide (HIP), produced in Korea, was fairly soluble over a wide pH rangein water-based solutions, whereas HI imported from Japan was insoluble except in strong acid and base solutions. Analysisusing an ultraviolet-visible spectrophotometer showed that the chromophore of HIP was shifted to the red compared with thatof HI. Fourier transform-infrared analysis revealed that HIP contained mainly amide (NH) groups, while HI largely containedamine (NH2) groups. With regard to constituents, between HIP and HI, their major components were different from each otheraccording to their ratio of fronts obtained by thin-layer chromatography. These results suggest that determination of solubil-ity should be included in the quality control process of HI products.
CD38 TRANSDUCES T CELL ACTIVATION SIGNALS THROUGH ASSOCIATED LCK
Kim, Uh-Hyun 이화여자대학교 세포신호전달연구센터 2000 고사리 세포신호전달 심포지움 Vol. No.2
CD38 ligation has been shown to induce activation of intracellular signaling cascade in T lymphocytes through Lck-dependent pathway. However, it is not clear how Lck initiates the CD38-mediated signaling process. In· the present study, we showed that CD38 and Lck were physically associated through the cytoplasmic tail and the SH2 domain, respectively. This was evidenced by coimmunoprecipitation of Lck with CD38 and Lck with isolated CD38 cytoplasmic domain from T cell lysate, cell lysate of COS-7 cells cotransfected with cDNAs of Lck and CD38, or mixture of in vitro translated CD38 and Lck. Since the CD38 cytoplasmic domain does not contain any tyrosine residue, the interaction should be independent of phosphotyrosine. The interaction was further confirmed by in vitro interaction between purified Lck SH2 domain and a non-phospho synthetic peptide corresponding to the membrane proximal region of CD38 cytoplasmic domain. In addition, CD38 ligation resulted in an elevated tyrosine kinase activity of the CD38-associated Lck and ultimate activation of IL-2 gene transcription. Furthermore, expression of a kinase deficient Lck mutant suppressed IL-2 gene activation in a dose dependent manner. These results strongly suggested that CD38 ligation indeed tranduced signals for T cell activation using its associated Lck.
Kim, Soo Hyun,Shin, Jong Hee,Kim, Eui-Chong,Lee, Kyungwon,Kim, Mi-Na,Lee, Won Gil,Uh, Young,Lee, Hye Soo,Lee, Mi-Kyung,Jeong, Seok Hoon,Jung, Sook In,Park, Kyung Hwa,Lee, Jin-Sol,Shin, Myung Geun,Suh, Oxford University Press 2009 Medical mycology Vol.47 No.3
<P>There have been very few multicenter studies of the relationship between the use of antifungals and resistance to them. We investigated the antifungal susceptibility of 1,301 clinical isolates of Candida collected from nine Korean hospitals during a 3-month period in 2006 to explore the existence of this type of relationship. Antifungal usage in the preceding year, defined as the daily dose per 1,000 patient days (DDD/1,000 PD), was calculated for each hospital. Resistance to fluconazole, itraconazole, and amphotericin B was detected in 2, 9, and 0.2% of the isolates, respectively. The MIC(50)/MIC(90) values were 0.03/0.125 mg/L for voriconazole, 0.06/0.25 mg/l for caspofungin, and 0.03/0.125 mg/l for micafungin. The total usage of systemic antifungals varied considerably among the nine hospitals, ranging from 6.1 to 96.2 DDD/1,000 PD. No relationship was found between the use of fluconazole (MIC> or =64 mg/l) or itraconazole (MIC> or =1 mg/l) and resistance in the Candida species (P>0.05). However, significant correlations were found between the percentage of Candida isolates that were non-susceptible to fluconazole (MIC> or =16 mg/l) and fluconazole usage (r=0.733, P=0.025) or total antifungal usage (r=0.767, P=0.016).</P>
A New Assay Method for Ornithine Aminotransferase
Kim, Uh Hyun,Kim, Hyung Rho,Lee, Gye Jeong,Park, Jin Woo 생화학분자생물학회 1986 BMB Reports Vol.13 No.4
An attempt was made to develop a new assay method for ornithine aminotransferase which catalyze the transfer of δ-amino group of ornithine to α-ketoglutamate-γ-semialdehyde and glutamate. Glutamate-γ-semialdehyde(or pyrroline-5'-carboxylic acid), one of the reaction products was found to produce a colored compound showing a peak absorbance at 510 ㎚ with ninhydrin in hot acidic solution, whereas ornithine and glutamate production and color intensity indicated that the enzyme activity could be properly assayed by reading the change of absorbance at 510 ㎚. The enzyme activity measured by new assay method was widely distributed in mouse tissues, especially high in small intestine, kidney and liver, and was mainly present in cytoplasmic fraction of small intestine and in mitochondrial fraction of liver. The enzyme activity showed increasing tendency in liver and kidney with age, in contrast to decreasing tendency in small intestine. The enzyme from hepatic mitochondrial fraction had nearly identical properties to that from intestinal cytoplasmic fraction, such as heat stability in the presence of pyridoxal phosphate, pH activity curves, Km values, for ornithine and α-ketoglutarate, suggesting that they are rather an identical-enzyme than isoenzymes.