8-hydroxyguanine이 전립선비대증 발생에 미치는 효과

이재원,이석영,이형래,이상철,김용태,김원재 충북대학교 의학연구소 2002 忠北醫大學術誌 Vol.12 No.2

연구목적: 전립선비대증은 노화현상에 의해 발생하는 질환으로서 임상적으로 주로 50세 이상에서 증상이 나타난다. 산소 라디칼은 DNA의 염기에 다양한 손상을 미칠 수 있다. 산소 라디칼에 의한 DNA의 변화에는 여러 가지 형태가 있으며 이러한 변화들 가운데서 guanine이 8-oxoguanine (8-hydroxyguanine, oh8Gua)으로의 수산화(hydroxylation) 반응은 가장 흔히 관찰되는 형태로서 노화 및 만성 퇴행성 질환, 기타 여러 질환의 중요한 병인으로 인식되고 있다. 세포에 유해한 영향을 주는 oh8Gua의 생성으로부터 유전자를 보호하는 수복효소 중 8-oxoguanine-DNA glycosylase (OGG1)가 주된 역할을 하는 것으로 알려져 있는데 이 효소를 생산하는 유전자인 hOGG1은 사람마다 유전자형이 다르다. 따라서 본 연구에서는 전립선비대증이 노화와 관련된 hOGG1 유전자형에 따라서 발생에 차이가 있는지를 조사하였다. 대상 및 방법: 충북대학교 병원에서 전립선비대증으로 진단 받고 입원하여 경요도전립선절제술을 받은 환자로 병리 조직학적으로 전립선비대증이 확인된 81명을 환자군으로 하였으며, 충청북도내 10개 군에서 50세 이상의 건강한 성인 남자 764명을 대상으로 실시한 전립선 무료검진에서 경직장 초음파 검사상 전립선 크기 20 gm 이하, 국제 전립선 증상 점수(IPSS) 7점 이하, 요속 10 ㎖/s 이상, 전립선 특이 항원(PSA) 4.0 ng/㎖ 이하인 81명을 대조군으로 설정하였다. 이들에서 채취한 혈액에서 genomic DNA를 얻은 후 PCR-SSCP (polymerase chain reaction-single stranded conformation polymorphism), 직접 염기서열분석(direct DNA sequencing) 및 RFLP (restriction fragment length polymorphism)를 통해서 두 군 사이의 hOGG1 유전자의 다형성을 분석하였다. 결과: 대조군에서 codon 326형은 Ser326Ser형이 12례(14.8%), Ser326Cys형이 51례(63.0%), Cys326Cys형이 18례(22.2%)였으며 전립선비대증 환자군에서는 Ser326Ser형이 15례(18.5%), Ser326Cys형이 34례(42.0%), Cys326Cys형이 32례(39.5%)로 두 군 사이에 통계적인 유의성이 관찰되었다(p=0.022). 또한 Cys326Cys형과 나머지(Ser326Ser, Ser326Cys)형으로 나누어 시행한 통계 분석에서도 대조군에서는 각각 18례(22.2%), 63례(77.8%)였으며 전립선비대증 환자군에서는 32례(39.5%), 49례(60.5%)로 통계적인 유의성이 관찰되었다(p=0.017, OR=2.286, 95% CI=1.149-4.546). 결론: hOGG1 유전자의 형태와 전립선비대증의 발생과는 밀접한 관련이 있었다. 즉 hOGG1 Cys326Cys형을 가진 사람에서 전립선비대증이 발생한 가능성이 높을 것으로 사료된다. Purpose: 8-oxoguanine DNA glycosylase (OSS1) rapairs DNA by removing 8-oxoguanine (oh8Gua), a highly mutagenic oxidative DNA adduct. Recently, the human gene for OGG1 (hOGG1) was cloned and several genotypes have been reported. However, the implications of such genotypes in benign prostatic lyperplasia have not been demonstrated. To assess the involvement of hOGG1 associated with the aging process on the developing of BPH, we analyzed the genetic polymorphisms of hOGG1. Materials and Methods: We studied the hOGG1 gene polymorphisms in 81 cases of BPH and 81 cases of normal controls. We performed the SSCP, PCR-based restriction fragment length polymorphism (RFLP) and direct sequencing to characterize the genetic polymorphisms of hOGG1 in both BPH patients and controls. Results: We found two polymorphic sites. A serine326/cysteine polymorphism at codon 326 (1a type) in exon 7 was associated with an exchange of amino acid. Another polymorphic site at codon 324 (1b type) in exon 6 was silent. We found that the distribution of hOGG1 genotypes in BPH patients (Ser326Ser type 18.5%, Ser326Cys type 42.0% and Cys326Cys type 39.5%) was significantly different from that in controls (14.8%, 63.0% and 22.2%, respectively) (p=0.022). Homozygosity for the Cys326Cys genotype significantly increased the risk of developing BPH, with the odds ratio (OR) being 2.286 (95% confidence interval [CI]=1.149-4.546) Conclusion: Our results suggest that the hOGG1 Cys326Cys genotype might play a important role in the development of BPH.

추황배(Pyrus pyrifolia Nakai cv. Chuhwangbae) 과피로부터 1종의 Sterol과 3종의 배당체 화합물의 단리·동정

이유건(Yu Geon Lee1),조정용(Jeong-Yong Cho),이현주(Hyun Joo Lee),이용현(Yong Hyun Lee),이상현(Sang-Hyun Lee),한태호(Tae-Ho Han),김월수(Wol-Soo Kim),박근형(Keun-Hyung Park),문제학(Jae-Hak Moon) 한국식품과학회 2013 한국식품과학회지 Vol.45 No.5

본 연구에서는 배의 유용성 증명을 위한 일환으로 배의 화학성분을 분자수준에서 밝히고자 하였다. 이에 배 과피 MeOH 추출물을 용매분획하여 얻은 EtOAc-산성 분획과 EtOAc-중성분획을 대상으로 Sephadex LH-20, silica gel, 그리고 ODS colmn chromatography와 HPLC를 이용하여 정제 및 단리하였다. 그 결과, EtOAc-산성 분획과 EtOAc-중성 분획으로부터 각각 2종씩의 화합물을 단리하였다. 단리된 화합물 1-4는 1H- 및 13C-NMR 분석을 통하여 각각 (S)-(+)-2-cis-abscisic acid O-β-D-glucopyranosyl ester (화합물 1), 1-[4-O-β-D-glucopyranosyl]phenyl ethanone(piceoside, 화합물 2), β-sitosterol (화합물 3), 그리고 β-sitosteryl 3-O-β-D-glucopyranoside (화합물 4)로 동정되었다. 단리된 3종의 배당체 화합물(화합물 1, 2, 4)들은 본 연구에 의해 배로부터 처음 동정되었으며, 화합물 3은 추황배로부터 처음 동정되었다. 본 연구결과가 배 함유 성분연구는 물론 배의 기능성 해명 연구에도 추후 중요한 기초자료로 활용되길 기대한다. We isolated and identified antioxidants from acidic and neutral ethyl acetate fractions of the peel of pear (Pyrus pyrifolia N. cv. Chuhwangbae). We isolated 4 compounds from the methanol extract, by using 3 different types of column chromatography (Sephadex LH-20, silica gel, and octadecylsilane) and preparative HPLC. We identified the isolated compounds as (S)-(+)-2-cis-abscisic acid O-β-D-glucopyranosyl ester (compound 1), 1-[4-O-β-D-glucopyranosyl]phenyl ethanone (picroside, compound 2), β-sitosterol (compound 3), and β-sitosteryl 3-O-β-D-glucopyranoside (compound 4) by nuclear magnetic resonance analysis. We are the first to report the identification of compounds 1, 2, and 4 from pear.

림프종 환자에서의 갈륨, TI-201 그리고 Tc-99m MIBI 섭취의 비교

천경아,조인호,원규장,이경희,이형우,현명수,이재태,이규보 영남대학교 의과대학 2002 Yeungnam University Journal of Medicine Vol.19 No.2

Purpose; Ga-67 scintigraphy has been used for the evaluation of tumors, especially lymphoma. Recently, Tl-201 and Tc-99m MIBI were also used to tumor imaging. Tl-201 and Tc-99m MIBI had better physiologic characteristics than Ga-67, so we studied 32 biopsy proven lymphoma patients (male 24, female 8, mean age 46 years) with Ga-67, Tl-201 or Tc-99m MIBI and compared the scan findings. Materials and Methods: Twenty-three of 32 patients were injected 74-111 MBq (2-3 mCi) of Tl-201, before chemotherapy and imaged with dual-headed SPECT (Prism 2000, Picker, USA) at 30 minutes after injection. Delayed images were obtained after 3 hr in 8 patients. Twenty seven of 32 patients were injected 740 MBq (20 mCi) of Tc-99m MIBI and imaged at 30 minutes after injection. 111-185 MBq (3-5 mCi) of Ga-67 was injected in 12 patients and imaged at 48 and 72 hours after injection. Twenty eight patients were diagnosed as non-Hodgkin's lymphoma and others were Hodgkin's lymphoma. Results: Twenty patients were positive on Tl-201 scan and 3 patients showed negative findings. One of these 3 patients, Tc-99m MIBI and Ga-67 scan were positive. Twenty two patients were positive on Tc-99m MIBI scan and 5 patients showed negative findings. One of these 5 patients, Tl-201 was positive and 2 were positive on Ga-67 scan. Ten of 12 patients showed positive findings on Ga-67 scan. The sensitivity of these agents were 83.3%, 87.0% and 81.5% for Ga-67, Tl-201 and Tc-99m MIBI, respectively. The sensitivity was highest in Tl-201 scan, but there were no significant differences among three tests. In this study, there was no significant difference of uptake ratios between early and delayed images of Tl-201. Conclusion: Scintigraphy with Tl-201 and Tc-99m MIBI in lymphoma patients have similar sensitivity with Ga-67.

Discordance in Spirometric Interpretations Based on Korean and Non-Korean Reference Equations

Nami Kim,Mi-Hee Park,Se-yeong Kim,Chunhui Suh,Sangyoon Lee,Kun-Hyung Kim,Chae-Kwan Lee,Dae-Hwan Kim,Jong-Tae Lee 대한직업환경의학회 2013 대한직업환경의학회지 Vol.25 No.-

Objectives: Korean regression models for spirometric reference values are different from those of other ethnic groups. The purpose of this study was to evaluate discordance in spirometric interpretations based on using Korean reference equations versus non-Korean reference equations. Methods: Spirometry was performed on 825 Korean male workers from April 2009 to November 2011. The spirometric patterns and disease severity were evaluated using two Korean equations (Choi’s and Lee’s) and three equations for Caucasians (NHANES III, Morris’s, and Knudson’s), and the results of Choi’s equation were compared with the non-Korean equations. The spirometric patterns were defined as normal, restrictive, and mild and moderate obstructive. Results: The mean differences in the FEV1% and FVC% between the two Korean equations were 2.0 ± 1.3% and 3.5 ± 2.2%, respectively. Morris’s equation had the greatest difference in the FEV1% from Choi’s equation: 32.9 ± 8.5%. Knudson’s equation had the greatest difference in the FVC% from Choi’s equation: 10.5 ± 6.5%. Conclusions: The two Korean equations shared similar characteristics of spirometric interpretation. The spirometric interpretations of Choi’s equation were significantly different from those of Morris’s equation and Knudson’s equation.

Al_2O_3를 첨가한 LaFeO_3 박막의 암모니아 가스감지 특성

이복상,조철형,최부천,박기철,마대영,김정규 경상대학교 생산기술연구소 2002 工學硏究院論文集 Vol.2 No.-

LaFeO_3 thin films with 2%, 5% and 10% A1_2O_3 additives were fabricated by r.f. magnetron sputtering method on Al_2O_3 substrates. Structural, electrical and ammonia gas sensing characteristics of the thin films with different heat treatments were examined. From the XRD results, the compound of LaFeO_3 and Al_2O_3 was not found. Thin film with 5% Al_2O_3 additives, heat-treated at 800℃, showed the sensitivities of about 85% for 100ppm ammonia gas at the working temperature of 300℃. The response time to the ammonia gas was several seconds and the thin film showed good selectivity to NH_3 gas.

敎科課程을 中心으로 한 韓日農學系(園藝·食品·獸醫)大學의 敎育現況 調査

李愚升,孫泰華,尹衡植,毛麒喆 경북대학교 1982 새마을 硏究論叢 Vol.2 No.-

The authors studied on the curicullum between Korea and Japan in the deaprtments of horiculture, food technology and veterinary. The results are as follows. 1. In the department of horticulture, professional education period was 3 years in Korea while 2 or 2.5 years in Japan. These results were compared among 3 colleges of Korea and Japan. Credits for graduation was 48-52% for propessional education with submajoring subjects and 45% when majoring subject in Korea, but 54-61% in Japan. Credits for required subjects were 17-29% among credits for graduation in Korea while 28-29% in Japan. 2. Cultural education period is almost 1 year in Korea, but different in Japan. Credits for graduation are as follows; Cultural education is 36-37%, majoring fields 15% and the others 12-13% in Korea while 43-46% for cultural education, 54-57% for majoring field in Japan. Differences were shown among 3 colleges dealt with the department of food technology by locational characteristics in Japan. 3. In the department of Veterinary, collage curicullums were not fixed for the students because of transitional period. Required subjects among 4 colleges were shown as the same tendency in Korea.

산딸나무 열매로부터 저밀도 지질 단백질(LDL) 산화 역제물질의 분리

이대영,송명종,김민정,정낙훈,정태숙,이윤형,백남인 경희대학교식량자원개발연구소 2007 硏究論文集 Vol.26 No.1

산딸나무 열매를 100% MeOH로 추출하고, 얻어진 추출물을 EtOAc, n-BuOH 및 물로 용매 분획 하였다. 이 중 EtOAc 분획으로부터 silica gel과 octadecyl silica gel(ODS) column chromatography를 반복하여 1종의 lignan 화합물을 분리, 정제하였다. 화합물의 화학구조는 NMR, Ms 및 IR 등의 스펙트럼 데이터를 해석하여, (+)-pinoresinol (1) 로 동정하였다. 이 화합물은 산딸나무에서 처음 분리되었다. LDL-항산화활성 억제활성이 20㎍/mL 처리농도에서 85.1±1.3%로 나타났다. (+)-Pinoresinol was isolated from the EtOAc fraction of methanol extracts of the fruits of Cornus kousa Burg. The chemical structures of (+)-pinoresinol was determined by spectroscopy method, as well as 1D- and 2D-NMR (^(1)H-^(1)H COSY, HSQC and HMBC) spectroscopy. This compound was the first to be isolated from C. kousa Burg. Also, (+)-pinoresinol inhibited LDL-oxidation with the inhibitory activity of 85.1±1.3% at a concentration of 20 ㎍/mL.

e-Learning을 위한 SCORM 기반 동적 시퀀싱

이종근,김형일,김준태 東國大學敎 産業技術硏究院 2005 산업기술논문집 Vol.15 No.1

e-learning에서 실행 환경에 종속적인 학습 컨텐츠는 저작 비용을 증가시키는 문제를 발생시킨다. 이와 같은 문제점을 해결하기 위해 SCORM에서는 학습 컨텐츠의 공유와 재사용에 관한 표준안을 제시한다. e-learning에서 또 다른 문제점은 학습자의 학업 성취도 향상에 있다. 현재 e-learning에서는 정형화된 교육 절차에 따라 학습을 수행하고 종료하는 방식을 취함으로써 학습자의 학업 성취도를 향상시키지 못한다. 이와 같은 문제점을 해결하기 위해 SCORM에서는 학습에 대한 절차를 규정하는 시퀀싱을 활용하여 학습자의 학업 성취도를 향상시킨다. 일반적으로 시퀀싱 설계는 교수자나 학습 저작자가 담당하여 학습 프로그램을 규칙화한다. 정형화된 시퀀싱으로는 학습 집단이나 학습자의 특성을 분석하기 어려우며, 잘못된 시퀀싱이 설계되었을 경우엔 학습자들의 학습에 악영향을 미친다. 본 논문에서는 이와 같은 문제점을 해결하기 위해 동적 시퀀싱을 적용한 학습 시스템을 제안한다. 동적 시퀀싱에서는 학습자들의 평가점수가 시퀀싱에서 활용되는 기준점수에 반영되어 기준점수를 동적으로 변화시킨다. 기준점수를 동적으로 변화시킴으로 시퀀싱은 학습 집단이나 학습자들의 학습 능력에 맞게 동적으로 변화되어 학습자의 학습 성취도를 향상시킨다. The problem occurred with the growth of e-learning lies in the different implementation environment and the learning contents which are not shared and reused. To solve these problems, SCORM presents a standard specification for the sharing and reusing of the learning contents. Another problem with the e-learning comes from the degree of improvement on learning accomplishment of learners. The present e-learning approach cannot improve the degree of learning accomplishment of learners because the learning is carried out and finished by formulated education procedures. To solve this problem, SCORM improved the degree of learning accomplishment of learners by utilizing the sequencing which regulates the procedures for learning. The formulated sequencing is difficult to analyze the characteristics of the learning group, and if a wrong sequencing is designed, it is ineffective on the learning of learners. In this paper, we proposed a learning system which adopted a dynamic sequencing so as to solve this problem.

Streptokinase와 Plasminogen의 활성 복합체에 대한 생화학적 특성

이현재,전형균,이해진,김태성,김연웅 慶尙大學校 1988 論文集 Vol.27 No.2

Fig 1과 Fig 2의 실험 결과에 의하면 streptokinase에 의한 plasminogen의 활성화 기작은 먼저 streptokinase와 plasminogen 결합하여 복합체를 만들고 그 다음 다른 분자의 streptokinase에 의한 plasminogen을 streptokinase에 의한 plasmin으로 활성화 한다고 생각되었다.그리고 원래 효소활성이 없는 streptokinase량이 plasminogen보다 과량으로 존재하게 되면 전체 esterolytic 활성도는 plasminogen 의 량에 의존하게 될 것이다.Fig 3의 실험에서 streptokinase plasminogen 복합체의 정량적인 구성 비율을 조사하였더니 plasminogen 1㎍에 0.5㎍의 streptokinase가 결합하였다. 이는 보고된 plasminogen의 분자량을 87.000(10) 그리고 streptokinase분자량이 47.000(6)으로 계산하면 각각 한 분자씩 결합하고 있음을 알 수 있었다. 이 결과는 Fig. 1의 결과를 보충할 수 있는 data이다. streptokinase plasminogen 복합체의 생화학적 특성을 조사하기 위하여 복합체만의 활성을 assay 할 수 있는 방법을 고안하였다. 실험결과 soybean trysin inhibitor를 사용하면 plasminogen의 활성화에 의하여 생성된 plasmin의 esterolytic 활성은 완벽하게 저해할 수 있었으나 streptokinase plasminogen 복합체의 활성은 저해하지 못하였으므로 soybean trypsin inhibitor를 첨가하여plasmin활성을 제거하여 순수한 복합체만의 활성을 측정할 수 있었다.(Fig.5).그러나 pancreatic tyrpsin inhibitor는 Plasmin 뿐만 아니라 streptokinase plasminogen복합체의 활성을 모두 저해할 수 있었으므로 이 inhibitor 는 복합체의 활성을 측정하는데 사용하지 않았다. (Fig.4). 두가지 inhibitor의 복합체에 대한 상이한 저해 효과는 복합체의 활성화 자리 (active site)에 두 inhibitor 가 결합하고난 후 기질이 출입할 수 있는 공간의 넓이에 의한 효과인 것으로 생각되었다 (Table 1).따라서 soybean trypsin inhibitor을 이용한 assay는 원래의 생화확적 특성에 영향이 없는 것으로 생각되었다. 복합체의 esterolytic 반응은 빠른 첫 번째 반응 그리고 그 이후 늦은 두 번째 반응으로 진행되는 biphasic한 형태를 보여 주었다(Fig.7).이 반응 기작은 알려진 serine protease들이 acyl중간체을 이용한 biphasic 반응 기작과 유사한 형태이었다. 활성화 자리에 위치하고 있는 여러 작용기들을 확인하기 위한 기초 실험으로써 pH에 따른 esterolytic활성을 측정한 결과 최대 활성을 보이는 pH는 7.5이었다.(Fig.8). 이 근처의 pKa 값을 가지고 아미노산은 histidine 종류을 생각할 수있을 것이다. The kinetic data suggest that streptokinase interacts with plasminogen to become an active complex which in turn. activates another molecule of plasminogen to plasmin. This complex was fromed stoichiometrically at 1:1 molar ratio. A series of experiment were carried out to find the condition that the streptokinase plasminogen complex activities free from plasmin could be monitered. The soybean trypsin inhibitor wsas used to assay the activity of streptokinase-plasminogen complex. The lack of inhibiton of esterase activity by soybean trypsin inhibitor was thought to be the size of the protein in active site. The esterolytic reaction by streptokinase-plasminogen complex showed biphasic pattern. The pH profile showed typical bell-shape and optimal pH for streptokinas-plasminogen complex showed biphasic pattern. The pH profile showed a typical bell-shape and optimal pH for streptokinas-plasminogen complex was 7.5.

Ⅲ-Ⅴ족 반도체의 연구 : GaAs의 합성, 단결성 및 특성 Crystal Growth and Characterization of GaAs

이형재,김영기,신영진,박해용,정양준,최춘태,김병호,김진성 全北大學校 基礎科學硏究所 1982 基礎科學 Vol.5 No.1

Temperature of 618 and 1250℃ at As and Ga sities respectively in the horizontal Bridgman furnace and growht speed of 0.7cm/h were found to be optimal for the production of single crystalline GaAs. Self-seeded and unintentionally doped GaAs crystals showd n-type characteristics with Hall mobillity of ∼0.75㎡/V-sec and carrier density of ∼10^19 m^-3 when the mesurements were made right after the growth. About three month later it was found that the same samples were converted to p-type GaAs. The analysis on the p-type sample showed that impurity scattering is dominant at around room temperature and thus the measured Hall mobility was 0.0065㎡/V-sec at 300K. 7.4±0.6 eV was obtained for acoustic deformation potential in the heavy hole band by fitting the theoretical conductivity and Hall coefficient to the corresponding experimental data. The value is very much higher than the theoretically estimated value, 3.6eV. The carrier density variation with temperature obtained from the present analysis gave 0.38 and 0.17 eV respectively for the activation energies of donor and acceptor.