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Chaetoglobosin A, an Inhibitor of Bleb Formation on K562 Cells Induced by Phorbol 12,13-Dibutyrate
KO, HACK-RYONG,KIM, BO YEON,AHN, SOON-CHEOL,OH, WON KEUN,KIM, JIN-HEE,LEE, HYUN SUN,KIM, HWAN-MOOK,HAN, SANG-BAE,MHEEN, TAE0ICK,AHN, JONG-SEOG 한국미생물 · 생명공학회 1998 Journal of microbiology and biotechnology Vol.8 No.6
Mheen, Tae Ick,Han, Moon H.,Seong, Baik Lin,Son, Hyeung Jin 생화학분자생물학회 1989 BMB Reports Vol.15 No.4
It was found that enzyme from a microbial strain, Monocillium sp. ATCC 20621 catalyzed the oxidative reaction of rifamycin B to form rifamycin O. The identification of the reaction products suggested that the reaction proceeded by the oxidative cyclization of rifamycin B to form rifamcycin O, which spontaneously hydrolyzed to rifamycin S in neutral aqueous milieu. The characteristics of enzyme was different as compared with that of other polyphenol oxidases such as lactase. It is proposed that this new type of enzyme be classified into subgroup EC 1. 10. 3. 6. with a trivial name rifamycin B oxidase.
Mheen, Tae Ick,Han, Moon H.,Seong, Baik L.,Park, Jong M.,Son, Hyeong J. 생화학분자생물학회 1989 BMB Reports Vol.15 No.4
Four kinds of enzyme preparations from Humicola sp. ATCC 20621 were used in this experiment, i.e., whole cell, defatted acetone dried powder, ammonium sulfate precipitate, and immobilized enzyme (defatted acetone dried powder entrapped in polyarylamide). The present enzyme was most specific for rifamycin B among various rifamycin derivatives and other similar golyol compounds. The Km values of soluble and immobilized enzyme for rifamycin B were 0.05 mM and 0.67 mM, respectively. The optimal pHs of soluble and immobilized enzyme for catalytic efficiency were 7.8 and 7.6, respectively, and the optimal temperatures were same in both of the enzymes. The enzyme activities were measured by two kinds of methods, dissolved oxygen consumption and spectrophotometric assay.
발효에 의한 라이신(L-lysine) 생산에 관한 연구(1) -라이신 생산균주의 분리 및 라이신 생산조건의 검토-
민태익,권태완,Mheen, Tae-Ick,Kwon, Tai-Wan 한국식품과학회 1971 한국식품과학회지 Vol.3 No.1
전국 각지에서 수집한 토양을 비롯한 기타 시료로부터 배양액중에 lysine을 생산 축적하는 미생물 94주를 분리하여 이를 다시 비교 검토함으로써 lysine생산이 2 mg/ml 이상인 균주 6주를 선정하고 균학적 성질을 조사한 결과 Micrococcus 속 1주, Corynebacterium 속 3주, Brevibacterium 속 2주였다. Corynebacterium sp. 1주 (S-27-12)에 대한 합성 배지에서의 lysine 축적조건을 검토한 결과 glucose 7.5%, urea 0.6%, $KH_2PO_4\;0.2%$, $Na_2HPO_4\;0.05%$, $MgSO_4{\cdot}7H_2O\;0.03%$, $MnSO_4{\cdot}4H_2O\;0.001%$, $FeSO_4{\cdot}7H_2O\;0.0005%$, pH 7.2가 최적이었으며 이 때의 lysine 생산량은 4mg/ml 정도였다. Ninty four strains of lysine producing micro-organisms in culture broth during fermentation have been isolated from soil and other sources. From the comparison of the amounts of lysine produced, 6 strains have been selected as the potentially useful strains, and identified tentatively as Micrococcus sp. (S-16-4), Corynebactcrium sp. (S-27-12, S-281-3, CBY-4) and Brevibacterium sp. (M-6-71, F-629-2), respectively. From the further studies with Corynebacterium sp., S-27-12, its maximum yield was found to be 4mg lysine/ml of synthetic medium, consist of glucose(7.5%), urea(0.6%), $KH_2PO_4(0.2%)$, $Na_2HPO_4(0.05%)$, $MgSO_4{\cdot}7H_2O(0.03%)$, $MnSO_4{\cdot}4H_2O(0.001%)$ and $FeSO_4{\cdot}7H_2O(0.0005%)$ at pH 7.2 and $30^{\circ}C$ after 4 days.