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Hibernation of Mammalian Cells at a Living Body Temperature
Hyon, Suong-Hyu,Kim, Do-Hoon The Korean Society for Biotechnology and Bioengine 2001 Biotechnology and Bioprocess Engineering Vol.6 No.4
The present study revealed that polyphenol induces the hibernation of mammalian cells at the living body temperature. It was found that polyphenol is a cytostatic sleeping agent for mam-malian cells, where almost all cells resume proliferation after the hibernation period and cell death seldom occurs. By changing the concentration for polyphenol, various mammalian cells can be stored under different conditions, such as temporary sleep, and hibernation condi-tions.
A Non-Frozen Living Tissue Bank for Allotransplantation Using Green Tea Polyphenols
Suong-Hyu Hyon 연세대학교의과대학 2004 Yonsei medical journal Vol.45 No.6
Generally, mammalian cells and living tissues can be cryopreserved in a frozen state at very low temperatures over a long storage term. The survival rate of cell suspensions is often acceptable however, living tissues suffer a variety of injuries. In this paper, it was demonstrated that the addition of polyphenols extracted from green tea to conventional cell culture medium and tissue compatible liquid, can control cell proliferation and also preserve tissues for several months at ordinary room temperature, including such tissues as blood vessels, cartilage, islet cells and corneas. This protocol allows a non-frozen living tissue bank to be established using the preservation fluid described.
이유빈,송수진,신용철,정유진,김봉주,강문성,권일근,Suong-Hyu Hyon,이현욱,정서현,임도형,한동욱 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.80 No.-
Recently, considerable research has been made on the development of bone tissue engineering (BTE)scaffolds to support cellular behaviors. Here, black phosphorus-incorporated poly(e-caprolactone) andcollagen (PCL/BP/Col) nanofiber matrices were designed and their potential as BTE scaffolds wereexplored. The PCL/BP/Col nanofiber matrices were fabricated successfully by electrospinning, showingthat they were suitable for supporting cell growth. The PCL/BP/Col nanofiber matrices could promote notonly initial attachment and proliferation, but also osteodifferentiation of preosteoblasts. In conclusion,PCL/BP/Col nanofiber matrices possess excellent biocompatibility and improve osteogenic differentia-tion. Therefore, the PCL/BP/Col nanofiber matrices can be a promising candidate as a scaffold for BTE.
Protection of Rabbit Kidney from Ischemia/Reperfusion Injury by Green Tea Polyphenol Pretreatment
나동균,한동욱,Hyun Sook Baek,Suong-Hyu Hyon,박병윤,박종철 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.11
Reactive oxygen species (ROS) have been implicated in the pathogenesis of renal injury after ischemia/reperfusion (I/R). Recently, green tea polyphenols (GTP) have been found to protect the myocardium and liver against II/R injury. Less attention, however, has been paid to the protective effects of GTP with respect to the kidneys. This study was designed to determine whether GTP could protect renal cells from ischemic injury. The rabbits were divided into three groups of equal size: control (sham-operated), I/R + vehicle (normal saline) and I/R + GTP groups. Each group consisted of six rabbits. Animals underwent 30, 60, 90 and 120 min of ischemia, followed by 24 h of reperfusion, respectively. GTP (200 microg/kg) or the vehicle was administered 45 min prior to commencement of I/R. The results demonstrated that GTP administration resulted in a significant (P < 0.05) reduction of renal damage after 90 min of ischemia, as indicated by the decreased levels of creatinine and urea nitrogen in serum. These results were confirmed by histological examinations, which showed that GTP pretreatment inhibited necrosis and sloughing of the proximal tubules induced by I/R. Examinations also showed decreased necrotic areas in the medulla and decreased glomerular collapse in the I/R-injured rabbits. Moreover, the infiltration of CD8+ T cells was considerably decreased in GTP-treated kidneys. The results of this study suggest that GTP can reduce renal injury by preventing the oxidative stress dependent on I/R and may be used in renal transplantation as an antioxidant.
( Dong Wook Han ),( Jung Yoon Bae ),( Suong Hyu Hyon ) 한국조직공학·재생의학회 2009 조직공학과 재생의학 Vol.6 No.1
Although epigallocatechin-3-O-gallate(EGCG), a major poly phenolic constituent of green tea, has various pharmacological and biological activities including anti-carcinogenic, anti-thrombotic and anti-inflammatory effects, relatively a little is known about its beneficial effects on the non-frozen preservation of mammalian cells and tissues. In this study, articular cartilages from human knee joint were pretreated with 1 mM EGCG for 1 d and then preserved in serum-free RPMI 1640 media with 1% antibiotic-antimycotic solution at 4℃ for 1, 2 and 4 wk. After cold preservation, chondrocyte viability(CCK-8 assay), biochemical and immunohistochemical composition[glycosaminoglycans and(type II)collagen], and biomechanical property(compressive elastic modulus) were assessed, respectively. Chondrocyte viability of cartilages pretreated with EGCG was significantly well-maintained for at least 2 wk with high contents of glycosaminoglycan and total collagen. These beneficial effects of EGCG pretreatment were more confirmed by histological and immunohistochemical observations showing well-preserved cartilaginous structures and delayed denaturation of the extracellular matrices in preserved specimens. The compressive elastic modulus(MPa) of cartilages pretreated with EGCG was well-maintained as much as that of fresh specimens without any increase as the progress in the preservation period. Here were also found that fluorescein isothiocyanate-conjugated EGCG were widely distributed through the matrix and clearly observed at the chondrocytes in the lacunas. Taking these results into consideration, it is suggested that EGCG may play an effective role in preserving articular cartilages, which be exploited to craft strategies for the long-term preservation of osteochondral allografts under cold storage conditions.
Han, Dong-Wook,Lee, Jun Jae,Jung, Duk-Young,Park, Jong-Chul,Hyon, Suong-Hyu Institute of Physics Pub 2009 Biomedical materials Vol.4 No.4
<P>Localized drug delivery from drug-eluting stents has been accepted as one of the most promising treatment methods for preventing restenosis after stenting. However, hypersensitivity reactions caused by their nonresorbable polymer coatings and bare-metal stents may result in serious clinical sequelae. Epigallocatechin-3-<I>O</I>-gallate (EGCG), the predominant catechin from tea, has been shown to exert anti-thrombotic, anti-inflammatory and anti-proliferative activities. In this study, it was hypothesized that sustainedly released EGCG from biodegradable poly(lactide-<I>co</I>-ϵ-caprolactone, PLCL) would suppress the proliferation of vascular smooth muscle cells (VSMCs). EGCG-releasing PLCL (E-PLCL) was prepared by blending PLCL with EGCG. The surface morphology, roughness and melting temperature of PLCL were not changed despite EGCG addition. EGCG was uniformly dispersed into E-PLCL and sustainedly released for periods up to 7 days by controlled diffusion rather than PLCL degradation. Moreover, EGCG did not affect tensile strength at break, but significantly increased the elastic modulus of PLCL. The proliferation of VSMCs onto E-PLCL was significantly suppressed although the cell attachment onto E-PLCL had been higher than that onto PLCL. On the other hand, EGCG-eluting polymeric stents were prepared with neither cracks nor webbings between struts, and their structural integrity was maintained without delamination or destruction. These results suggest that E-PLCL can be potentially applied for fabricating an EGCG-eluting vascular stent, namely an EGCG-eluting polymeric stent, or even an EGCG-releasing polymer-coated metal stent, to prevent thrombosis, inflammation and in-stent restenosis.</P>