Effects of donor age on human adipose-derived adherent stromal cells under oxidative stress conditions

Kim, Sang Woo,Choi, Jung-Won,Lee, Chang Youn,Lee, Jiyun,Shin, Sunhye,Lim, Soyeon,Lee, Seahyoung,Kim, Il-Kwon,Lee, Hoon-Bum,Hwang, Ki-Chul Cambridge Medical Publications Ltd 2018 The Journal of international medical research Vol.46 No.3

<P><B>Objective</B></P><P>Adipose-derived stromal vascular fractions (SVFs) are heterogeneous complex populations of cells with therapeutic efficacy for tissue generation and vascular stabilization. SVFs have cardiomyogenic potential, and many researchers have examined the possibility of SVF transplantation for heart disease. In cell-based therapies, donor age affects the regenerative capability, cell yield, and differentiation potential of adult tissues; however, opposing or controversial results have been found in humans. We examined whether SVF transplantation into impaired heart tissue shows differential effects according to donor age.</P><P><B>Methods</B></P><P>We investigated differences in protein expression in human umbilical vein endothelial cells (HUVECs) co-cultured with adipose-derived adherent stromal cells (ADASs) from donors of different ages [>40-year-olds (40s group) and >60-year-olds (60s group)] under oxidative stress conditions.</P><P><B>Results</B></P><P>Although co-culturing HUVECs with ADASs ameliorated inflammation due to increased oxidative stress conditions, few differences were observed between the ADASs from the 40s and 60s groups. Moreover, the Database for Annotation, Visualization, and Integrated Discovery classification tool revealed differentially expressed genes in the Kyoto Encyclopedia of Genes and Genomes pathway associated with cytokine–cytokine receptor interaction in response to ADASs.</P><P><B>Conclusion</B></P><P>Protein expression profiles were unchanged in HUVECs induced by isolated ADASs from donors of different ages under oxidative stress conditions.</P>

Fluoroethylene Carbonate Addition Effect on Electrochemical Properties of Mixed Carbonate-based Organic Electrolyte Solution for a Capacitor

Kim, Mingyeong,Kim, Ick-Jun,Yang, Sunhye,Kim, Seok Korean Chemical Society 2014 Bulletin of the Korean Chemical Society Vol.35 No.2

In this paper, organic solvent electrolytes were prepared by a mixture of propylene carbonate (PC), dimethyl carbonate (DMC), tetraethylammonium tetrafluoroborate ($TEABF_4$)s to evaluate the ionic properties of propylene carbonate (PC)/dimethyl carbonate (DMC) mixtures as solvents for a capacitor application, in view of improving the electrochemical performances. The bulk resistance and interfacial resistance of the mixture electrolytes were investigated using an AC impedance method. The morphology of carbon-based electrodes which were contained in different electrolytes was analyzed by scanning electron microscopy (SEM) method. From the experimental results, by increasing the FEC content, capacitance of electrodes was increased, and the interfacial resistance was decreased. In particular, by a content of 2 vol % FEC in 0.2 M $TEABF_4$ PC/DMC solvent, the electrolyte showed the superior capacitance. However, when FEC content exceeds 2 vol %, the capacitance was decreased and the interfacial resistance was increased.

Source apportionment of PM_2.5 using positive matrix factorization (PMF) at a rural site in Korea

Kim, Sunhye,Kim, Tae-Young,Yi, Seung-Muk,Heo, Jongbae Elsevier 2018 Journal of environmental management Vol.214 No.-

<P><B>Abstract</B></P> <P>The sources of different pollutants contributing to ambient fine particles (PM<SUB>2.5</SUB>) on Daebu Island, Korea, were estimated. Twenty four hour integrated filter samples were collected from May 21–November 1, 2016, and analyzed for organic carbon, elemental carbon, ions, and trace elements. Positive matrix factorization was conducted on the PM<SUB>2.5</SUB> chemical speciation data from the samples to define the pathways and sources of PM<SUB>2.5</SUB> at the sampling site. A total of 80 samples and 24 chemical species were used to run the model and a total of nine sources were identified: secondary sulfate (29.0%), mobile (22.0%), secondary nitrate (13.2%), oil combustion (10.1%), coal combustion (9.4%), aged sea salt (7.9%), soil (5.6%), non-ferrous smelting (1.7%), and industrial activity (1.1%). Conditional probability and potential source contribution functions were then used to determine whether these sources were local or came from pollutants transported over long-range distances. The anthropogenic sources came from local emissions and originated from both industrialized and metropolitan areas, whereas the secondary inorganic aerosols were strongly influenced by the long-range transport of air pollutants from Shandong and Jiangsu provinces in China.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Source apportionment of ambient PM<SUB>2.5</SUB> is investigated in background area of Korea. </LI> <LI> Air pollutants from China enhance secondary inorganic aerosols contributions. </LI> <LI> Primary sources are effected by local sources of industrial and metropolitan areas. </LI> </UL> </P>

Isolation of a Tryptophan - Overproducing Strain Generated by EMS Mutagenesis of Candida rugosa

Sunhye Kim,Yumi Ha,Hyoungjoon Youm,Gilhwan An,Bongduk Lee,Misun Won,Kyung Bin Song 한국식품영양과학회 2004 Preventive Nutrition and Food Science Vol.9 No.2

To isolate a mutant strain which overproduces tryptophan, mutants of Candida rugosa were screened after EMS (ethyl methane sulfonate) mutagenesis. Fluorotryptophan, a tryptophan analogue, was used for selection of a tryptophan-overproducing mutant after mutagenesis. Among 50 mutants, several candidates were selected based on intracellular tryptophan content. Amino acid analysis results showed that C3 was the best strain because it had the highest amount of tryptophan among the mutants.

Electrochemical properties of organic electrolyte solutions containing 1-ethyl-3-methylimidazolium tetrafluoroborate salt

Kim, Mingyeong,Kim, Ick-Jun,Yang, Sunhye,Kim, Seok Springer-Verlag 2015 Research on chemical intermediates Vol.41 No.7

Aggregation behavior of carborane-poly(ethylene glycol) complexes at the air- water interface

Sunhye Kim,Jingying Yang,Chi Wu,Daewon Sohn 한국고분자학회 2016 한국고분자학회 학술대회 연구논문 초록집 Vol.41 No.2

Polymorphic expression of DAZ proteins in the human testis

Kim, Byunghyuk,Lee, Youngbin,Kim, Yeonwha,Lee, Kyung Ho,Chun, Sunhye,Rhee, Kunsoo,Seo, Ju Tae,Kim, Soo Woong,Paick, Jae-Seung Oxford University Press 2009 Human reproduction Vol.24 No.6

<P>BACKGROUND:DAZ is a male infertility gene located at the AZFc region of the Y chromosome. There are four copies of the DAZ gene that share a strong homology but are not identical to one another. In the present study, we carried out cDNA cloning and immunoblot analyses to determine whether all of the DAZ genes are actively expressed in the human testis. METHODS:AZFc deletion was detected by sequence-tagged site polymerase chain reaction (PCR) of genomic DNA isolated from blood samples. DAZ cDNAs were cloned with RT-PCR followed by sequence analysis. The expression of DAZ proteins in human testis was determined by immunoblot and compared with DAZ cDNA expression. RESULTS:Immunoblot analysis revealed four DAZ protein bands in testis samples that showed no deletions in the AZFc region. No specific bands were observed in samples from AZFc deletion patients. Testis samples from individuals with the partial AZFc deletion, gr/gr, showed two DAZ-specific bands. Interestingly, the sizes of DAZ-specific bands varied among individuals. Analysis of DAZ transcripts in testis samples revealed that the DAZ proteins were translated from the largest of the multiple transcripts originating from each single DAZ gene. CONCLUSIONS:All four DAZ genes are expressed in the human testis, and their products are highly polymorphic among men.</P>

Multiplex PCR−Based Next-Generation Sequencing and Global Diversity of Seoul Virus in Humans and Rats

Kim, Won-Keun,No, Jin Sun,Lee, Seung-Ho,Song, Dong Hyun,Lee, Daesang,Kim, Jeong-Ah,Gu, Se Hun,Park, Sunhye,Jeong, Seong Tae,Kim, Heung-Chul,Klein, Terry A.,Wiley, Michael R.,Palacios, Gustavo,Song, Ji U.S. Department of Health and Human Services * Cen 2018 Emerging Infectious Diseases Vol.24 No.2

<P>Seoul virus (SEOV) poses a worldwide public health threat. This virus, which is harbored by <I>Rattus norvegicus</I> and <I>R. rattus</I> rats, is the causative agent of hemorrhagic fever with renal syndrome (HFRS) in humans, which has been reported in Asia, Europe, the Americas, and Africa. Defining SEOV genome sequences plays a critical role in development of preventive and therapeutic strategies against the unique worldwide hantavirus. We applied multiplex PCR–based next-generation sequencing to obtain SEOV genome sequences from clinical and reservoir host specimens. Epidemiologic surveillance of <I>R. norvegicus</I> rats in South Korea during 2000–2016 demonstrated that the serologic prevalence of enzootic SEOV infections was not significant on the basis of sex, weight (age), and season. Viral loads of SEOV in rats showed wide dissemination in tissues and dynamic circulation among populations. Phylogenetic analyses showed the global diversity of SEOV and possible genomic configuration of genetic exchanges.</P>

Trends in health behaviors over 20 years: findings from the 1998-2018 Korea National Health and Nutrition Examination Survey

Soyeon Kim,Sunhye Choi,Jihee Kim,Suyeon Park,Youngtaek Kim,Ok Park,Kyungwon Oh 한국역학회 2021 Epidemiology and Health Vol.43 No.-

OBJECTIVES: This study aimed to examine the trends in health behaviors in Korean population using data from the Korea National Health and Nutrition Examination Survey (KNHANES). METHODS: The subjects were 96,408 adults aged 19 years or older who participated in the first (1998) through seventh (2016-2018) KNHANES health interview. The prevalence of health behaviors (cigarette smoking, alcohol drinking, and physical activity) and annual percent change (APC) were estimated using SAS and the Joinpoint program. RESULTS: The prevalence of current cigarette smoking in men decreased by 2.8%p (APC=-2.8, p<0.001) annually over the 20-year period, and the prevalence of exposure to secondhand smoke at home substantially decreased compared to 2005 (APC=-8.8, p<0.001). Compared to 2005, the prevalence of current alcohol drinking in women, but not men, increased (APC=2.0, p<0.001), and the prevalence of binge drinking decreased in men (APC=-0.7, p<0.001) and increased in women (APC=2.4, p<0.001). The prevalence of aerobic physical activity decreased from 2014 in both gendersd (p<0.001). The prevalence of healthy behaviors practice (non-smoking, alcohol abstinence, and aerobic physical activity) was down-trending (APC=-5.3, p<0.001), especially among women (APC=-6.4, p<0.001). CONCLUSIONS: Over the past 20 years, smoking behaviors improved. However, drinking behavior was unchanged and physical activity indicators markedly decreased. More active programs are necessary for improving health behaviors, which are major risk factors linked to chronic diseases.

구어체 발화 동사 ‘얘기하다’ 구문 연구

김선혜(Sunhye Kim) 우리말학회 2023 우리말연구 Vol.75 No.-

이 글은 말뭉치 용례 분석을 바탕으로 구어에서 주로 쓰이는 발화 동사 ‘얘기하다’의 다양한 구문을 살피고 그 특성을 기술하는 것을 목적으로 한다. 이를 위해 다양한 ‘얘기하다’ 구문을 발화의 상대자를 상정할 수 있는 기본 구문과 발화의 상대자를 상정하지 않는 기타 구문으로 나누어 살폈다. 다시 기본 구문에서는 자·타동 구문과 인용 구문으로 나누어 명사 ‘이야기’의 의미가 확장되는 양상을 고찰하였다. 기타 구문에서는 발화 양식을 한정하거나 발화의 결과를 나타내는 구문으로 ‘얘기하다’가 일반 발화 동사로서 의미 확장이 일어나는 양상을 살펴보았다. 또한 관련 구문으로 ‘얘기(를) 하다’를 함께 비교해 보았다. 이를 통해 ‘얘기하다’가 구어체 발화 동사로서 ‘말하다’와 유사한 일반 발화 동사로 발달하였음을 보였다. Based on the analysis of corpus, this article aims to examine various construction of the utterance verb ‘얘기하다' that mainly used in spoken language and describe its characteristics. To this end, various '얘기하다' construction were divided into basic construction that can assume the counterpart of the utterance and other construction that do not assume the counterpart of the utterance. Again, in the basic syntax, the meaning of the noun '이야기(story)' was expanded by dividing it into self- and other syntax and citation syntax. In other construction, we examined the pattern in which the meaning of '얘기하다' as a general utterance verb occurs as a construction that limits the manner of speech or indicates the result of the utterance. We also compared '얘기하다' with other related construction. Through this, it was shown that '얘기하다' was a colloquial utterance verb that expanded its meaning in the meaning of the noun 이야기(story)' and developed into a general utterance verb similar to '말하다'.