http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Preparation and in vivo evaluation of immediate-release pellet containing celecoxib solid dispersion
Chun-Woong Park,Nguyen-Thach Tung,Dao-Danh Son,김주영,이윤석,Seung-Yeop Kang,Shin-Ae Park,황규목,오택운,하정명,Sang-Cheol Chi,박은석 한국약제학회 2012 Journal of Pharmaceutical Investigation Vol.42 No.3
The aim of this study was to make use of small size of immediate-release (IR) pellet and amorphous state of solid dispersion to increase solubility of celecoxib (CLX), a drug in BCS class II. Primary, binary and ternary solid dispersions were developed to choose the final components for solid dispersion. A ternary novel solid dispersion was prepared by incorporation of one aqueous soluble polymer (povidone k17; PVP 17PF), Methacrylate copolymer-based gastric soluble polymer (Eudragit EPO) and one pH modulator (MgO). This combination was effective to increase solubility in pH 1.2 up to 25–30 %. The mechanismof solubility enhancement was proven by DSC, PRXD, and FT-IR. Accordingly, hydrogen bonding or electrostatic interaction of CLXwithPVP/EudragitEPOwas themain cause to formthe amorphous state of CLX within polymer cluster which increasing solubility of drug. Besides, MgO played an important role to change microenviroment for solid dispersion. Pellets containing this solid dispersion were prepared by extrusion and spheronization technique. Effect of four kinds of additive (calciumhydrogen phosphate dihydrate,NaHCO3,crospovidone, and sodium dodecyl sulfate) on dissolution of CLX from IR pellet was also determined. Because of highest dissolution rate, formulation using sodium dodecyl sulfate was used for pharmacokinetics study. Solid dispersion-IR pellet formulation presented bioequivalence and lower variability in comparison with reference product.
Shin, Jae-Yoon,Yang, Yoosoo,Heo, Paul,Lee, Ji-Chun,Kong, ByoungJae,Cho, Jae Youl,Yoon, Keejung,Shin, Cheol-Su,Seo, Jin-Ho,Kim, Sung-Gun,Kweon, Dae-Hyuk Dove Medical Press 2012 International journal of nanomedicine Vol.7 No.-
<P><B>Background</B></P><P>Nanoparticles undergoing physicochemical changes to release enclosed drugs at acidic pH conditions are promising vehicles for antitumor drug delivery. Among the various drug carriers, high-density lipoprotein (HDL)-like nanoparticles have been shown to be beneficial for cancer chemotherapy, but have not yet been designed to be pH-responsive.</P><P><B>Methods and results</B></P><P>In this study, we developed a pH-responsive HDL-like nanoparticle that selectively releases paclitaxel, a model antitumor drug, at acidic pH. While the well known HDL-like nanoparticle containing phospholipids, phosphatidylcholine, and apolipoprotein A-I, as well as paclitaxel (PTX-PL-NP) was structurally robust at a wide range of pH values (3.8–10.0), the paclitaxel nanoparticle that only contained paclitaxel and apoA-I selectively released paclitaxel into the medium at low pH. The paclitaxel nanoparticle was stable at physiological and basic pH values, and over a wide range of temperatures, which is a required feature for efficient cancer chemotherapy. The homogeneous assembly enabled high paclitaxel loading per nanoparticle, which was 62.2% (w/w). The molar ratio of apolipoprotein A-I and paclitaxel was 1:55, suggesting that a single nanoparticle contained approximately 110 paclitaxel particles in a spherical structure with a 9.2 nm diameter. Among the several reconstitution methods applied, simple dilution following sonication enhanced the reconstitution yield of soluble paclitaxel nanoparticles, which was 0.66. As a result of the pH responsiveness, the anticancer effect of paclitaxel nanoparticles was much more potent than free paclitaxel or PTX-PL-NP.</P><P><B>Conclusion</B></P><P>The anticancer efficacy of both paclitaxel nanoparticles and PTX-PL-NP was dependent on the expression of scavenger receptor class B type I, while the killing efficacy of free paclitaxel was independent of this receptor. We speculate that the pH responsiveness of paclitaxel nanoparticles enabled efficient endosomal escape of paclitaxel before lysosomal break down. This is the first report on pH-responsive nanoparticles that do not contain any synthetic polymer.</P>
Analysis of the Soluble Human Tooth Proteome and Its Ability to Induce Dentin/Tooth Regeneration
Chun, So Young,Lee, Hyo Jung,Choi, Young Ae,Kim, Kyung Min,Baek, Sang Heum,Park, Hyo Sang,Kim, Jae-Young,Ahn, Jung-Mo,Cho, Je-Yeol,Cho, Dong-Woo,Shin, Hong-In,Park, Eui Kyun Mary Ann Liebert 2011 Tissue engineering. Part A Vol.17 No.1
Cyclic Testing of Exterior Beam-Column Joints with Varying Joint Aspect Ratio
Chun, Sung-Chul,Shin, Yeong-Soo American Concrete Institute 2014 ACI structural journal Vol.111 No.3
<p>To examine the effects of joint aspect ratio (beam depth to column depth ratio) on shear strength and required amount of transverse reinforcement in exterior beam-column joints, 14 joint specimens were tested under reversed cyclic loading. The main experimental parameters were the joint aspect ratio, the amount of transverse reinforcement, and the anchorage types of beam bars (hooked and headed bars). The test results indicate that, for a joint aspect ratio equal to or less than 1.0, all joints showed typical flexural behavior. Even transverse reinforcement was reduced to two-thirds of the transverse reinforcement required by ACI 352R-02; the hysteretic behavior and strengths of the joints were similar to those of the joints designed in accordance with ACI 352R-02. For a joint aspect ratio equal to or greater than 2.0, nominal strengths of joints could not be developed and joint shear failure occurred where the requirements of ACI 352R-02 were satisfied. From the analysis of measured shear strengths of the joints, it is suggested that the shear strength factor of ACI 352R-02 is reduced as the joint aspect ratio increases, if the joint aspect ratio is higher than 1.65. The test results also indicate that there was no significant difference between anchorage types of hooked or headed bars.</p>
( Sung Hwan Lee ),( Ju-seog Lee ),( Yun Shin Chun ) 대한간학회 2020 춘·추계 학술대회 (KASL) Vol.2020 No.1
Aims: Intrahepatic cholangiocarcinoma (IHC) is the second most common primary liver cancer. There is no clinical consensus for the management of multiple tumors in the intrahepatic cholangiocarcinoma. The aim of this study is to evaluate the spatio-temporal evolutions of multiple tumors that belong to the same patients and provide the clinically relevant evidence for precision therapeutic strategies in the intrahepatic cholangiocarcinoma. Methods: A total of 34 tumors from nine patients were analyzed by next-generation sequencing using custom-designed targeted gene panel with the deep targeted platform. Single nucleotide variants with indel aberration, somatic mutations, and copy number alterations were obtained from bioinformatics computational analysis. Results: All multiple tumors in each patient showed the signify cant similarity of somatic mutation with indel pattern and copy number alteration. The analysis for spatio-temporal evolution using clustering cancer evolutionary trees revealed that 8 out of 9 patients have multiple tumors from genetically same clonal origin even in different anatomical locations of the liver. We identified shared driver mutations in each patient such as BAP1, IDH1, and BRAF. The only patient not having shared non-synonymous somatic mutation showed a significantly concordant pattern of copy number alteration in multiple tumors. Conclusions: Genomic profiles were concordant among all tumors in each patient, suggesting a common progenitor cell origin regardless of the location in the liver. Most of the patients were identified to share the actionable genetic alteration commonly in multiple tumors. Our results support the development of molecular-targeted therapeutic strategies in multifocal intrahepatic cholangiocarcinoma.
Sang-Tae Choi,Shin Hwang,Hea-Nam Hong,You-Jin Won,Chul-Soo Ahn,Tae-Yong Ha,Gi-Won Song,Dong-Hwan Jung,Gil-Chun Park,Sung-Gyu Lee 한국간담췌외과학회 2013 한국간담췌외과학회지 Vol.17 No.1
Backgrounds/Aims: Mesenchymal stem cells (MSCs) have the capacity to differentiate into hepatocytes, The purpose of this study is to investigate the MSCs’ differentiation process and therapeutic potentials by comparing isolated MSCs with HGF-treated MSCs in rat’s model with thiacetamide (TAA)-induced cirrhosis. Methods: Male Sprague-Dawley (SD) rats, weighing 100-150 g were used in this study. To induce liver fibrosis, recipient rats were taken with 0.04% thioacetamide (TAA) in the drinking water (400 mg TAA/L) for 8 weeks. The rats underlying liver cirrhosis were divided into 3 groups according to the transplanted materials, compared to normal saline as control (I) and isolated MSCs (II) HGF-treated MSCs. Results: Severe hepatic fibrosis and hepatocyte destruction were detected in the control group. Less hepatic cirrhosis and collagen formation, more hepatocyte regeneration and glycogen storage were detected in isolated MSCs compared to HGF-treated MSCs group, Distribution of red autofluorescence is mainly localized near the sinusoids in isolated MSCs, scattered away the sinusoids in HGF-treated MSCs group. MSCs transdifferentiated into CK-19 postive Oval cells and then to albulmin-producing hepatocytes, HGF treated MSCs differentiated into hepatocyte without the intermediate oval cells phase. HGF treated MSCs became the CK18-positive, MSCs became CD 90-positive. Conclusions: Significant hepatocyte differentiation occurred in not HGF-treated MSCs but isolated MSCs group unexpectedly. These results suggest that the beneficial effect of MSCs on in rat’s model with TAA-induced cirrhosis may occur during early differentiation course of MSCs. Mature hepatocyte itself has a little effect on the accelerated differentiation and functional capacity of hepatic lineage cell-line.