건강가정지원센터 가정생활교육 프로그램 운영 및 성과 : 용산구·숙명여자대학교 시범사업을 중심으로

김명자,계선자,박미석,장진경,김연화,한은주,류진아 한국가족관계학회 2005 한국가족관계학회지 Vol.10 No.1

The purpose of this study is to propose a model for service programs and management strategies for the division of family education in Healthy Families Center. For the purpose, the researchers find out demands for the family education programs of people in Yong-San Gu, Seoul, exam the effect of each family education program of Yong-San Gu & Sookmyung Women's University Healthy Families Center from June to December, 2004. According to the results, people in Yong-San Gu need economic education service mostly, and they want to study at Saturday afternoon. The programs show positive effect on the improvement of family health.

The Oxford classification as a predictor of prognosis in patients with IgA nephropathy.

Kang, Seok Hui,Choi, Sun Ryoung,Park, Hoon Suk,Lee, Ja Young,Sun, In O,Hwang, Hyeon Seok,Chung, Byung Ha,Park, Cheol Whee,Yang, Chul Woo,Kim, Yong Soo,Choi, Yeong Jin,Choi, Bum Soon Springer International ; Oxford University Press 2012 Nephrology, dialysis, transplantation Vol.27 No.1

<P>In 2009, the Oxford classification was developed as a pathological classification system for immunoglobulin A nephropathy (IgAN) to predict the risk of disease progression. The aim of this retrospective study was to evaluate the clinical and pathologic relevance of the Oxford classification in Korean patients with a pathologic diagnosis of IgAN.</P>

Comparison of Clinical Outcomes by Different Renal Replacement Therapy in Patients with End Stage Renal Disease Secondary to Lupus Nephritis

( Seok Hui Kang ),( Byung Ha Chung ),( Sun Ryoung Choi ),( Ja Young Lee ),( Hoon Suk Park ),( In O Sun ),( Bum Soon Choi ),( Cheol Whee Park ),( Yong Soo Kim ),( Chul Woo Yang ) 대한내과학회 2011 The Korean Journal of Internal Medicine Vol.26 No.1

Background/Aims: Many studies have compared patients with systemic lupus erythematosus (SLE) on renal replacement therapy (RRT) with non-lupus patients. However, few data are available on the long-term outcome of patients with end-stage renal disease (ESRD) secondary to SLE who are managed by different types of RRTs. Methods: We conducted a retrospective multicenter study on 59 patients with ESRD who underwent maintenance RRT between 1990 and 2007 for SLE. Of these patients, 28 underwent hemodialysis (HD), 14 underwent peritoneal dialysis (PD), and 17 patients received kidney transplantation (KT). We analyzed the clinical outcomes in these patients to determine the best treatment modality. Results: The mean follow-up period was 5 ± 3 years in the HD group, 5 ± 3 years in the PD group, and 10 ± 5 years in the KT group (p = 0.005). Disease flare-up was more common in the HD group than in the KT group (p = 0.012). Infection was more common in the PD and HD groups than in the KT group (HD vs. KT, p = 0.027; PD vs. KT, p = 0.033). Cardiovascular complications were more common in the HD group than in the other groups (p = 0.049). Orthopedic complications were more common in the PD group than in the other groups (p = 0.028). Bleeding was more common in the HD group than in the other groups (p = 0.026). Patient survival was greater in the KT group than in the HD group (p = 0.029). Technique survival was lower in the PD group than in the HD group (p = 0.019). Conclusions: Among patients with ESRD secondary to SLE, KT had better patient survival and lower complication rates than HD and lower complication rates than PD. The prognosis between the HD and PD groups was similar. We conclude that if KT is not a viable treatment option, any alternative treatment should take into account the patient`s general condition and preference. (Korean J Intern Med 2011;26:60-67)

Session 2. 곤충매개 인수공통전염병: Vectorborne Zoonotic Diseases : S2-2 ; Severe Fever with Thrombocytopenia Syndrome: Molecular and Serological Diagnosis

( Sun Whan Park ),( Jung Sang Ryou ),( Seok Min Yun ),( Chan Park ),( Won Ja Lee ),한명국 ( Myung Guk Han ) 대한인수공통전염병학회 2014 창립총회 및 학술대회 초록집 Vol.2014 No.1

Severe fever with thrombocytopenia syndrome (SFTS) is a new emerging viral infectious disease, first reported in China in 2010. Patients with SFTS have been reported recently in South Korea and Japan in 2013. Totally 36 cases of SFTS were identified in Korea in 2013 with case fatality rate of 47%. The SFTS is characterized by acute febrile illness, thrombocytopenia, leucopenia, gastrointestinal symptoms, elevated serum enzymes and multi-organ failure which are not specific signs and symptoms for SFTS. The SFTS virus (SFTSV) causing SFTS belongs to the genus Phlebovirus in the family Bunyaviridae. Heartland viruses and Hunter Island Group virus (HIGV) which are related to but distinctly different from SFTSV were isolated from patients in the US and ticks in Australia, respectively. The patients infected with Heartland virus presented a similar signs and symptoms to SFTS. HIGV was isolated from ticks collected from shy albatross on Albatross Island, a small island in the Hunter Island Group in northwestern Tasmania, Australia. The SFTSV has been detected in Haemaphysalis longicornis and Rhipicephalus microplus ticks suggesting that the causative agent of SFTS, SFTSV is transmitted possibly to humans by ticks, such as H. longicornis which is considered as the principal vector of SFTSV. Recently Amblyomma testudinarium and Ixodes nipponensis are also implicated as the vector of SFTSV. Although cases of person-to-person transmission through contact with infected patient’s blood or mucous have also been reported in China, transmission of SFTSV takes place by biting of ticks infected with SFTSV. The SFTS presents with clinical manifestations similar to those of other infectious vector-borne diseases, such as hemorrhagic fever with renal syndrome (HFRS), scrub typhus, leptospirosis and anaplasmosis, strongly suggesting the need for differential diagnosis of SFTS from other infectious diseases. HFRS, leptospirosis and anaplasmosis are caused by Hantavirus, Leptospira interrogans and Anaplasma phagocytophilum, respectively. Wild rodents (Apodemus agrarius) play the role of the primary natural reservoir for these pathogens. HFRS, scrub typhus and leptospirosis are endemic in eastern and south-east Asia including Korea. In terms of distribution of hosts and reservoirs of vector-borne pathogens in the environment, and current coexistence of these diseases in the same epidemic area, concurrent infections of these vector-borne diseases can occur. Therefore, a reliable SFTSV detection tool is urgently required to provide early diagnosis of SFTS to support clinical care, infection control and epidemiological surveillance. Laboratory diagnosis of SFTSV infection is carried out by various ways, including nucleic acid amplification, detection of viral antigen, virus isolation and antibody detection to SFTSV using by real-time RT-PCR, Vero E6 cell culture, enzyme-linked immunosorbent assay (ELISA) and indirect fluorescent assay (IFA). Conventional RT-PCR and real-time RT-PCR was developed in our laboratory and applied to detect SFTSV from hospitalized patients who presented SFTS symptoms. The SFTSVs were detected from 35 specimens among more than 300 serum specimens in 2013 and the nucleotide sequence was analyzed for identification of the SFTSV. The Korean isolates of SFTSV showed 93-98% similarityof the nucleotide sequences to Chinese and Japanese isolates of SFTSV and were distinctly different from Heartland virus. SFTSVs were also isolated by Vero E6 cell culture and identified by the nucleotide sequence, IFA using monoclonal antibodies, and electron microscopy. As reported, antibody to SFTSV can be detected as early as 2 to 4 days after illness onsets by serological assays and persists in some patients even one year after recovery. Seroconversion against SFTS V in patients with SFTS occurs mostly more than 3 weeks after onsets of illness. We developed IFA for laboratory diagnosis and determined serologic cross reactivity of SFTSV to Hantavirus and rickets. Slides for IFA were prepared with Vero E6 cells infected with SFTSV and Hantaan virus (HTNV). Commercial IFA kits for Anaplasma, Ehrlichia, Leptosira and Origentia spp were also used in the study. Serum specimens of SFTSV patients, sera of HTNV IgG antibody of more than 512 and paired sera of scrub typhus patients were tested with IFA. None of sera specimens showed specific antibody reaction to SFTSV infected cells and antigens assayed by IFA. IgG titers to each homogeneous antigen of HTNV and O.tsutsugamushi assayed by IFA were ranged from 512 to 4,096 and from 0 to 8,192. These results suggest that SFTSV does not have cross reactivity to at least, HTNV and O. tsutsugamushi. Considered currently occurrence of SFTSV, HTNV and O. tsutsugamushi in the same epidemic of the country, concurrent infection can be identified by serological assays. In the presentation, we report a case of coinfection with SFTSV and Hantavirus in humans. To the best of our knowledge, this is the first case report of coinfection with SFTSV and Hantavirus. A 66-year-old farm-dwelling woman was admitted to the hospital with a 6-day history of worsening fever and myalgia. Neutropenia and thrombocytopenia were evident on admission to the intensive care unit. SFTSV infection was suspected based on clinical findings and laboratory test results, although the patient had no recollection of a tick bite and there was no evidence of tick bites. She was treated with plasma exchange and oral ribavirin (4.0g/day) after 10 days of illness onset and had fully recovered at 15 days after illness onset Considering the concurrence of SFTS, HFRS and ricketiisial diseases in the endemic area and the higher possibility of exposure to pathogens due to the patient’s area of residence and occupation as a farmer, coinfection with SFTSV, hantavirus and ricketiisial agents in the patient is suggested. Determining the effects of coinfection on disease prognosis and laboratory diagnosis could be helpful in deciding patient treatment and management.

Clinical Research Poster Presentation : Simultaneous hard and soft tissue augmentation for implant-supported restorations

( Sun Ja Gu ),( Hwa Suk Seok ) 대한치주과학회 2009 대한치주과학회 학술대회자료집 Vol.2009 No.1

Safety and Immunogenicity of Salmonella enterica Serovar Typhimurium LlaB in Mice

( Sun A Cho ),( In Soo Lee ),( Jong Hwan Park ),( Seung Hyeok Seok ),( Hui Young Lee ),( Dong Jae Kim ),( Min Won Back ),( Seok Ho Lee ),( Sook Jin Hur ),( Sang Ja Ban ),( Yoo Kyoung Lee ),( Jae Hak P 한국미생물생명공학회 2005 Journal of microbiology and biotechnology Vol.15 No.3

Implementation of IDGPS Based Car Tracking System Using Internet and Mobile Phone

Sun-Jun Ko(고선준),Hee-Seok Ho(호희석),Jong-Hoon Won(원종훈),Ja-Sung Lee(이자성) 대한전기학회 2001 대한전기학회 학술대회 논문집 Vol.2001 No.7

Severe Fever with Thrombocytopenia Syndrome Virus, South Korea, 2013

Park, Sun-Whan,Han, Myung-Guk,Yun, Seok-Min,Park, Chan,Lee, Won-Ja,Ryou, Jungsang U.S. Department of Health and Human Services * Cen 2014 Emerging Infectious Diseases Vol.20 No.11

<P>During 2013, severe fever with thrombocytopenia syndrome was diagnosed in 35 persons in South Korea. Environmental temperature probably affected the monthly and regional distribution of case-patients within the country. Phylogenetic analysis indicated that the isolates from Korea were closely related to isolates from China and Japan.</P>

Molecular genomic characterization of tick- and human-derived severe fever with thrombocytopenia syndrome virus isolates from South Korea

Yun, Seok-Min,Park, Su-Jin,Park, Sun-Whan,Choi, WooYoung,Jeong, Hye Won,Choi, Young-Ki,Lee, Won-Ja Public Library of Science 2017 PLoS neglected tropical diseases Vol.11 No.9

<▼1><P><B>Background</B></P><P>Severe fever with thrombocytopenia syndrome (SFTS) is an emerging tick-borne viral disease caused by the SFTS virus (SFTSV) from <I>Bunyaviridae</I> that is endemic in East Asia. However, the genetic and evolutionary characteristics shared between tick- and human-derived Korean SFTSV strains are still limited.</P><P><B>Methodology/Principal findings</B></P><P>In this study we identify, for the first time, the genome sequence of a tick (<I>Haemaphysalis longicornis</I>)-derived Korean SFTSV strain (designated as KAGWT) and compare this virus with recent human SFTSV isolates to identify the genetic variations and relationships among SFTSV strains. The genome of the KAGWT strain is consistent with the described genome of other members of the genus <I>Phlebovirus</I> with 6,368 nucleotides (nt), 3,378 nt, and 1,746 nt in the Large (L), Medium (M) and Small (S) segments, respectively. Compared with other completely sequenced human-derived Korean SFTSV strains, the KAGWT strain had highest sequence identities at the nucleotide and deduced amino acid level in each segment with the KAGWH3 strain which was isolated from SFTS patient within the same region, although there is one unique amino acid substitution in the Gn protein (A66S). Phylogenetic analyses of complete genome sequences revealed that at least four different genotypes of SFTSV are co-circulating in South Korea, and that the tick- and human-derived Korean SFTSV strains (genotype B) are closely related to one another. Although we could not detect reassortant, which are commonly observed in segmented viruses, further large-scale surveillance and detailed genomic analysis studies are needed to better understand the molecular epidemiology, genetic diversity, and evolution of SFTSV.</P><P><B>Conclusions/Significance</B></P><P>Full-length sequence analysis revealed a clear association between the genetic origins of tick- and human-derived SFTSV strains. While the most prevalent Korean SFTSV is genotype B, at least four different genotypes of SFTSV strains are co-circulating in South Korea. These findings provide information regarding the molecular epidemiology, genetic diversity, and evolution of SFTSV in East Asia.</P></▼1><▼2><P><B>Author summary</B></P><P>Severe fever with thrombocytopenia syndrome (SFTS) is an emerging tick-borne viral disease caused by the SFTS virus (SFTSV). During entomological surveillance of SFTSV infection in Korean ticks collected from SFTS outbreak areas, we isolated a single SFTSV strain which we designated KAGWT. In addition, we isolated three SFTSVs from human patients with typical SFTS symptoms. In this study, we report the genomic sequences of each of these isolates and compare the genetic and evolutionary characteristics between tick- and human-derived Korean SFTSV isolates. Genetic and phylogenetic analyses of these sequences revealed that the tick-derived Korean SFTSV strain is clustered into genotype B, the most prevalent genotype in South Korea, and was closely related to other SFTSV in the same group. Furthermore, our results show that at least four different genotypes of SFTSV strains are co-circulating in South Korea.</P></▼2>

Dentin bond strength of bonding agents cured with Light Emitting Diode

Kim, Sun-Young,Lee, In-Bog,Cho, Byeong-Hoon,Son, Ho-Hyun,Kim, Mi-Ja,Seok, Chang-In,Um, Chung-Moon 大韓齒科保存學會 2004 Restorative Dentistry & Endodontics Vol.29 No.6

This study compared the dentin shear bond strengths of currently used dentin bonding agents that were irradiated with an LED (Elipar FreeLight, 3M-ESPE) and a halogen light (VIP, BISCO). The optical characteristics of two light curing units were evaluated. Extracted human third molars were prepared to expose the occlusal dentin and the bonding procedures were performed under the irradiation with each light curing unit. The dentin bonding agents used in this study were Scotchbond Multipurpose (3M ESPE), Single Bond (3M ESPE), One-Step (Bisco), Clearfil SE bond (Kuraray), and Adper Prompt (3M ESPE). The shear test was performed by employing the design of a chisel-on-iris supported with a Teflon wall. The fractured dentin surface was observed with SEM to determine the failure mode. The spectral appearance of the LED light curing unit was different from that of the halogen light curing unit in terms of maximum peak and distribution. The LED LCU (maximum peak in 465 ㎚) shows a narrower spectral distribution than the halogen LCU (maximum peak in 487 ㎚). With the exception of the Clearfil SE bond (P < 0.05), each 4 dentin bonding agents showed no significant difference between the halogen light-cured group and the LED light-cured group in the mean shear bond strength (P > 0.05). The results can be explained by the strong correlation between the absorption spectrum of camphoroquinone and the narrow emission spectrum of LED. LED 광중합기(Elipar FreeLight, 3M-ESPE)와 할로겐 광중합기(VIP, Bisco)로 광조사한 수종의 상아질 접 착제에 대해서 상아질 전단접착강도를 비교하였다. 또한 이번 연구의 광학적 근거를 얻기 위해 두 광중합기의 파장 에 따른 광강도와 스펙트럼을 비교하였다. 이번 연구에서 사용된 상아질 접착제는 Scotchbond Multipurpose (3M ESPE), Single Bond (3M ESPE), One-Step (Bisco), Clearfil SE Bond (Kuraray), Adper Prompt (3M ESPE) 이다. VIP는 487 ㎚에서 최대정점을 가지는 넓은 스펙트럼의 분포를 보이는 반면에, Elipar FreeLight는 465 ㎚에서의 최대정점을 중심으로 좁은 스펙트럼의 분포를 보였다. Clearfil SE bond를 제외하고 할로겐과 LED로 광조사한 각 상아질 접착제에서 상아질 전단접착강도값의 유의성 있는 차이는 보이지 않았다 (P > 0.05). 이러한 결과는 camphoroquinone의 흡수스펙트럼과 LED의 좁은 영역의 스펙트럼사이에 강한 연관성으로 설명할 수 있다.