Expression of CD44 according to Clinicopathologic Characteristics of Gastric Cancer

Min Sun Ryu,Hee Jung Park,Chang Mo Moon,Seong-Eun Kim,Hye-Kyung Jung,Ki-Nam Shim,Sung-Ae Jung,Min Sun Cho 이화여자대학교 의과학연구소 2018 EMJ (Ewha medical journal) Vol.41 No.3

Objectives: Cancer stem cells are defined as focal cluster of cells within a tumor that possess the capacity for self-renewal and differentiation into phenotypically heterogeneous cells. Cluster of differentiation 44 (CD44) is considered one of the gastric cancer stem cell markers. We aimed to investigate how the expression of CD44 varies according to the clinicopathologic characteristics in gastric cancer. Methods: For this study, 157 patients who received an operation due to gastric cancer between May 1998 and December 2009 were selected. CD44 immunohistochemistry was reviewed using the semi-quantitative scoring of intensity and proportion. The sum of the intensity and proportion scores was calculated, and a score of 2 or less was deemed ‘CD44-negative’ and 3 or more as ‘CD44-positive.’ Results: Among the final 143 subjects, 69 (48.3%) were CD44 positive. Older age, intestinal type gastric cancer, lymphatic invasion, and lymph node metastasis were significantly correlated with expression of CD44. In the multivariate analysis, older age was the only independent factor associated with CD44 expression (P=0.028). CD44 expression was correlated with overall survival, 5-year survival, and diseasefree survival. In the multivariate analysis, older age, male gender, and lymphatic invasion were independent predictors of poor overall survival. Also, older age and lymphatic invasion were significant factors in 5-year survival, and lymphatic invasion was an independent factor of poor disease-free survival. Conclusion: Older age (≥60 years) was independently associated with CD44 expression in gastric cancer patients. Also, CD44 expression was correlated with poor prognosis in gastric cancer patients.

Clinical Dimensions of Bloating in Functional Gastrointestinal Disorders

( Min Sun Ryu ),( Hye-kyung Jung ),( Jae-in Ryu ),( Jung-sook Kim ),( Kyung Ae Kong ) 대한소화기기능성질환·운동학회(구 대한소화관운동학회) 2016 Journal of Neurogastroenterology and Motility (JNM Vol.22 No.3

Background/Aims Bloating is common bothersome symptoms and most studies conducted in the Western countries found that bloating was frequently associated with lower gastrointestinal (GI) symptoms but many patients complaint bloating as upper GI symptoms in the clinical setting. This study was conducted to assess the prevalence of bloating, and to identify symptom grouping and finally document the impact of bloating in the diagnosis of functional GI disorders. Methods Participants in a comprehensive health-screening cohort were enrolled. They were asked about demographic, medical, and social history and upper and lower GI symptoms by using a validated questionnaire. Factor analysis with principal component analysis method with varimax rotation was used. Results Among the total of 1050 subjects (mean age, 44.6 ± 10.2 years; females, 46.4%), significant bloating symptoms were found in 282 (26.9%); the prevalence of functional bloating was 6.9%. Factor analysis revealed a 5-component structure with upper GI symptoms, constipation, diarrhea-predominant irritable bowel syndrome (IBS), constipation-predominant IBS, and fecal incontinence. Abdominal bloating loaded on both the upper GI symptoms (0.51 of loadings) and constipation (0.40). On logistic regression analysis, bloating was more predictable for IBS (OR, 7.5; P < 0.001) than functional dyspepsia (FD; OR, 3.7; P = 0.002). Bloating was more frequently combined with IBS according to their severity, but this association was not detected in patients with FD. Conclusions Abdominal bloating is common symptom in about a quarter of patients and appears as upper as well as lower GI symptoms. However, abdominal bloating is more predictable for IBS, especially constipation-predominant IBS, than FD. (J Neurogastroenterol Motil 2016;22:509-516)

벤자틴 페니실린에 의해 유도된 국소 발작간 간질양 파형이 백서 대뇌에서 c-JUN 단백질 발현 양상에 미치는 영향

김선국,이용만,방선웅,이애영,김재문,유택근,이영호 대한신경과학회 2000 대한신경과학회지 Vol.18 No.5

노년기 주관적 기억력 감퇴의 예측인자 : 한국인의 건강과 노화에 관한 종적 연구 결과

유지민,김기웅,박준혁,이석범,최은애,최진영,정지운,도연자,노현아,박영선,이동영,우종인 大韓神經精神醫學會 2007 신경정신의학 Vol.46 No.6

Objectives : We investigated the predictors of subjective memory complaints in the community-dwelling normal elderly. Methods : This study was conducted as a part of Korean Longitudinal Study on Health and Aging (KLoSHA). 747 non-demented community-dwelling elderly aged 65 years or older were recruited. All participants underwent clinical evaluation for dementia and psychiatric disorder conformed to the Korean version of the Consortium to Establish a Registry for Alzheimer's Disease (CERAD-K.) Clinical Assessment Battery and Korean version of Mini-Intemational Neuropsychiatric Interview, res-pectively. Word list recall test, frontal assessment battery, Mini Mental Status Examination (MMSE-KC) and Korean version of Geriatric depression scale (GDS-K) were administered to evaluate episodic memory, frontal function, global cognition and depression, respectively. Subjective memory complaint was defmed in two different ways: worse than one's past (SMC-P) and worse than others of one's age (SMC-O). Results : In highly educated elderly, minor depressive disorder (0R=7.23, 95% C.I.= 2.29-22.86) and frontal dysfunction (0R=2.48, 95% C.I.= 1.29-4.77) significantly increased the risk of SMC-O. However, they did not influence the risk of SMC-P. In low educated elderly, both the minor depressive disorder and frontal dysfunction did not influence the risk of SMC-0 as well as that of SMC-P. Conclusion : SMC-O can be a sensitive subjective recognition of mild depression and/or frontal dysfunction in highly edu-cated normal elderly.

난소암에서 종양억제유전자들의 Multiple Methylator Phenotypes

류효충,조치흠,권상훈,박준철,서민애,정선욱,이정호,김종인,윤성도,차순도 대한부인종양 콜포스코피학회 2003 Journal of Gynecologic Oncology Vol.14 No.3

지금까지 알려진 바로는 종양억제유전자와 관련해서 CpG island의 methylation 이상으로 종양억제유전자의 기능 변화가 암 형성에 관여한다고 알려져 있다. 최근의 연구 결과 대장암이나 유방암에서 이러한 hypermethylation이 진단적 지표자로서의 사용가능성을 제시하였다. 따라서 난소암 환자에서 이러한 있는 종양억제유전자들의 CpG islands의 methylation의 변화를 알아보고자 하는 연구가 시도되고 있다. 지금까지 난소암에서 methylation에 관한 연구는 많이 행해져 있지 않아 본 연구자는 난소암에서 종양억제 유전자인 BRCA1, p15, p16, p21, p73, hMLH1의 methylation유무를 알아보고자 연구를 계획하였다. 난소암 13예와 정상 난소 조직 7예를 이용하여 p15, p16, p21, p73, BRCA1, hMLH1유전자들에 대한 MSPCR을 시행하였다. p15 유전자는 난소암과 정상 난소에서 모두가 methylation을 보이지 않았고, hMLH1에서는 모두에서 같은 methylation을 보여 의의가 없었다. p21 유전자는 양군 모두에서 같은 정도의 methylation을 보였고, p73 유전자는 난소암 13예 중에서 11예에서 methylation이 관찰되었으며, 정상 난소에서는 모두에서 methylation이 있었으나, 난소암에서 보다는 methylation이 적었다. p16 유전자는 난소암에서 13예 중 10예에서 methylation이 보였으나 정상에서도 methylation이 모두 관찰되었다. BRCA1 유전자는 난소암에서 11/13 (85%)에서 methylation이 있었고, 정상에서는 4/7 (57%)에서 methylation이 나타나 차이를 확인하였다. 이상의 결과로 저자가 확인한 난소암에서의 methylation 이상은 BRCA1 유전자에서 정상과 비교하여 차이를 확인하였으며, BRCA1 유전자의 methylation 변화가 난소암의 암화 과정에 관계한다는 것을 유추할 수 있겠다. 그러나 표본 수가 적은 관계로 향후 좀더 많은 난소암과 대조군을 가지고 이러한 MSPCR 방법을 이용해 종양억제유전자의 변이나 발현 감소를 적절한 표적 CpG islands를 찾아 시행하면 좋은 결과를 보게 되리라 생각된다. Objective : Recent evidence suggests that aberrant methylation of CpG islands is a major pathway leading to the inactivation of tumor suppressor genes and development of cancer. The purpose of this study was to detect the methylation in ovarian cancer. Methods : Recent studies on colorectal and breast cancer have defined a CpG island methylator phenotype, which involves the targeting of multiple genes by promotor hypermethylation. Little is currently known about the role of methylation in ovarian cancer. To detect the methylation in ovarian cancer, we have investigated the methylation status of 13 primary ovarian cancers at six genes using methylation specific polymerase chain reaction compared with 7 normal ovaries. Results : Six of tumor suppressor genes (p15, p16, p21, p73, BRCA1, and hMLH1) were evaluated to see the methylation status. Methylation of p15, p21, p73, and hMLH1 did not detect in ovarian cancers compared with normal ovaries. Ten of 13 ovarian cancers showed methylation of p16 gene and all normal ovaries showed hypermethylation. The BRCA1 gene was methylated in 11 (85%) of 13 ovarian cancers, 4 (57%) of 7 normal ovaries. Conclusion : Methylation of the BRCA1 gene is common alteration in ovarian cancers, and may paly a part of role in pathogenesis of ovarian cancer.

고도비만 환자에서 복강경 위소매절제술 후 발생한 누공의 내시경 치료 1예

류민선,심기남,조원영,김찬용,강현주,김미연,안소영,이윤표,조형원,정성애,이주호 이화여자대학교 의과학연구소 2013 EMJ (Ewha medical journal) Vol.36 No.2

Laparoscopic sleeve gastrectomy can reduce morbidity and mortality in patients with morbid obesity, but it can cause complications such as a gastrointestinal leak. A 30-year-old morbidly obese female who had type 2 diabetes mellitus and hypertension with estimated body mass index of 40.2 kg/m2 was admitted. Laparoscopic sleeve gastrectomy was performed. On postoperative day 19, a leak was suspicious on physical examination and radiologic findings. Conservative management was performed, but the patient was hemodynamically unstable and imminently septic. After laparoscopic drainage procedure, esophagogastroduodenoscopy was performed and revealed the fistula opening at staple line just below gastroesophageal junction. Fibrin tissue adhesive was injected around the fistula and the esophageal covered stent was inserted to cover the leak. At 14th days after stent insertion, the barium study confirmed no more leak. In this case, we experienced that the esophageal stent insertion with fibrin tissue adhesive injection may reduce recovery time of the fistula developed after laparoscopic sleeve gastrectomy

Tristetraprolin Inhibits the Growth of Human Glioma Cells through Downregulation of Urokinase Plasminogen Activator/Urokinase Plasminogen Activator Receptor mRNAs

Ryu, Jinhyun,Yoon, Nal Ae,Lee, Yeon Kyung,Jeong, Joo Yeon,Kang, Seokmin,Seong, Hyemin,Choi, Jungil,Park, Nammi,Kim, Nayoung,Cho, Wha Ja,Paek, Sun Ha,Cho, Gyeong Jae,Choi, Wan Sung,Park, Jae-Yong,Park, Korean Society for Molecular and Cellular Biology 2015 Molecules and cells Vol.38 No.2

Urokinase plasminogen activator (uPA) and urokinase plasminogen activator receptor (uPAR) play a major role in the infiltrative growth of glioblastoma. Downregulatoion of the uPA and uPAR has been reported to inhibit the growth glioblastoma. Here, we demonstrate that tristetraprolin (TTP) inhibits the growth of U87MG human glioma cells through downregulation of uPA and uPAR. Our results show that expression level of TTP is inversely correlated with those of uPA and uPAR in human glioma cells and tissues. TTP binds to the AU-rich elements within the 3' untranslated regions of uPA and uPAR and overexpression of TTP decreased the expression of uPA and uPAR through enhancing the degradation of their mRNAs. In addition, overexpression of TTP inhibited the growth and invasion of U87MG cells. Our findings implicate that TTP can be used as a promising therapeutic target to treat human glioma.

Obesity and the Metabolic Syndrome in Korean Adolescents

Ryu, So Yeon,Kweon, Sun-seog,Park, Hyung-chul,Shin, Jun-ho,Rhee, Jung-ae The Korean Academy of Medical Sciences 2007 JOURNAL OF KOREAN MEDICAL SCIENCE Vol.22 No.3

<P>This study evaluated the prevalence of metabolic syndrome and investigated its association with being overweight in Korean adolescents. Data were obtained from 1,393 students between 12 and 13 yr of age in a cross-sectional survey. We defined the metabolic syndrome using criteria analogous to the Third Report of the Adult Treatment Panel (ATP III) as having at least three of the following: fasting triglycerides ≥100 mg/dL; HDL <50 mg/dL; fasting glucose ≥110 mg/dL; waist circumference >75th percentile for age and gender; and systolic blood pressure >90th percentile for age, gender, and height. Weight status was assessed using the age- and gender-specific body mass index (BMI), and a BMI ≥85th percentile was classified as overweight. Of the adolescents, 5.5% met the criteria for the metabolic syndrome, and the prevalence increased with weight status; it was 1.6% for normal weight and 22.3% in overweight (<I>p</I><0.001). In multivariate logistic regression analyses among adolescents, overweight status was independently associated with the metabolic syndrome (odds ratio, 17.7; 95% confidence interval, 10.0-31.2). Since childhood metabolic syndrome and obesity likely persist into adulthood, early identification helps target interventions to improve future cardiovascular health.</P>

The effects of different attentional focus on poststroke gait

Sun-Ae Kim,Young Uk Ryu,Hwa Kyung Shin 한국운동재활학회 2019 JER Vol.15 No.4

The Clinical Association of the Blood Pressure Variability with the Target Organ Damage in Hypertensive Patients with Chronic Kidney Disease

Ryu, Jiwon,Cha, Ran-hui,Kim, Dong Ki,Lee, Ju Hyun,Yoon, Sun Ae,Ryu, Dong Ryeol,Oh, Ji Eun,Kim, Sejoong,Han, Sang-Youb,Lee, Eun Young,Kim, Yon Su The Korean Academy of Medical Sciences 2014 JOURNAL OF KOREAN MEDICAL SCIENCE Vol.29 No.7

<P>It is known that blood pressure variability (BPV) can independently affect target organ damage (TOD), even with normal blood pressure. There have been few studieson chronic kidney disease (CKD) patients. We evaluated the relationship between BPV and TOD in a cross-sectional, multicenter study on hypertensive CKD patients. We evaluated 1,173 patients using 24-hr ambulatory blood pressure monitoring. BPV was defined as the average real variability, with a mean value of the absolute differences between consecutive readings of systolic blood pressure. TOD was defined as left ventricular hypertrophy (LVH) (by the Romhilt-Estes score ≥4 in electrocardiography) and kidney injury (as determined from an estimated glomerular filtration rate [eGFR]<30 mL/min/1.73 m<SUP>2</SUP> and proteinuria).The mean BPV of the subjects was 15.9±4.63 mmHg. BPV displayed a positive relationship with LVH in a univariate analysis and after adjustment for multi-variables (odds ratio per 1 mmHg increase in BPV: 1.053, <I>P</I>=0.006). In contrast, BPV had no relationship with kidney injury. These data suggest that BPV may be positively associated with LVH in hypertensive CKD patients.</P><P><B>Graphical Abstract</B></P><P/>