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RISS 인기검색어
- 검색키워드
- 저자 : Suhee Kim (검색결과 168 건)
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Kim, Suhee,Park, Jeong Won,Wark, Alastair W.,Jhung, Sung Hwa,Lee, Hye Jin American Chemical Society 2017 ANALYTICAL CHEMISTRY - Vol.89 No.22
<P>The multiplexed detection of protein biomarkers in plasma present over a range of clinically relevant concentrations continues to be difficult for surface-based bioaffinity detection platforms such as surface plasmon resonance (SPR). As well as nonspecific adsorption, challenges include quantitative comparison between targets whose concentrations differ by orders of magnitude, regenerating SPR chips after plasma exposure, and the two- or four-channel limitation of many commercial SPR instruments limiting sample throughput. In this article, we explore an approach where two protein biomarkers alpha-1 antitrypsin (AAT) and Tau 381 are detected in tandem within a single SPR channel at micromolar and femtomolar concentrations, respectively. This was achieved by creating a mixed antibody (antiAAT and antiTau) monolayer on the chip surface. After the adsorption of AAT and/or Tau, further specificity was obtained via the adsorption of a DNA aptamer specific to each target. The detection range for each target was controlled via the relative surface density ratio of each antibody type as well as each aptamer concentration. Calibration measurements were performed in both buffer and spiked plasma with the detection of native concentrations of ∼39 fM (Tau) and ∼65 μM (AAT) in a human plasma sample. Finally, tandem measurements of both targets within the same SPR signal channel were demonstrated at these very different concentrations.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/ancham/2017/ancham.2017.89.issue-22/acs.analchem.7b03837/production/images/medium/ac-2017-03837q_0006.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ac7b03837'>ACS Electronic Supporting Info</A></P>
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Kim, Suhee,Ahn, Sun Hee,Yang, Hee-Young,Lee, Jin-Sil,Choi, Hyang-Gi,Park, Young-Kyu,Lee, Tae-Hoon Informa UK (TaylorFrancis) 2017 Redox report Vol.22 No.6
<P>Discussion: Pg appears to be redox-sensitive in cancer, and the C457 modification may impair cell migration and proliferation by affecting its interaction with the E-cadherin/catenin axis. Our findings suggest that redox-sensitive cysteines of Pg may be the targets for CRC therapy.</P>
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Kim, Suhee,Lee, Hye Jin American Chemical Society 2017 ANALYTICAL CHEMISTRY - Vol.89 No.12
<P>A new sandwich assay for tetracycline (TC) involving a DNA aptamer and antibody pair is demonstrated in conjunction with gold nanostar (GNS) enhanced surface plasmon resonance (SPR) to achieve detection in the low attomolar range. GNS particles were covalently functionalized with the antibody probe (antiTC) and integrated into a surface sandwich assay in conjunction with a SPR gold chip modified with the TC-specific aptamer. After it was demonstrated that both affinity probes can bind simultaneously to TC, optimization of the assay was performed using either antiTC only or GNS-antiTC conjugates to interact with aptamer/TC complexes present on the chip surface. Target concentrations as low as 10 aM could be detected using GNS-antiTC's, which was >10(3) times greater in performance than when using antiTC only. In addition, good selectivity was achieved with respect to other tetracycline derivative antibiotics, such as oxytetracycline (OTC) and Chlortetracycline (CTC), both which are structurally similar to TC. As a demonstration of trace antibiotic analysis in environmental samples, the GNS enhanced sandwich assay was applied to analyze TC added to aliquots of local river water and the results validated by comparing to conventional high-performance liquid chromatography (HPLC) analysis.</P>
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Stress Coping and Resilience in College Students with Depression
Kim Dahni,Yoon Bo-Hyun,Sea Young-Hwa,Kang Hangoeunbi,Kim Kyungmin,Song Jye-Heon,Park Suhee 대한우울조울병학회 2021 우울조울병 Vol.19 No.3
Background: Depression is increasing among college students in general. Moreover, almost one-third of college students have been reported to suffer from depression. Thus, this study aimed to assess differences in stress coping strategies and resilience between depressed and normal-mood groups among college students. Methods: A total of 3,306 college students participated in this study. The students responded to a questionnaire that included questions of the Center for Epidemiologic Studies Depression (CES-D) scale, stress coping scale (SCS), and brief resilience scale. Logistic regression analysis was conducted to evaluate the impact of variables on depression. Results: Using the CES-D (cutoff score ≥21), 423 (12.8%) college students were classified as depressed. Adjusting for individual demographic factors, the SCS results of the students with depression showed significantly higher scores in emotion-focused coping (p<0.001), wishful thinking (p<0.001), and lower problem-focused coping (p<0.001) than the normal-mood group. Moreover, they presented lower resilience scores. Students who had emotion-focused coping (odds ratio [OR], 1.11; p<0.001) and lower resilience scores (OR, 0.76; p<0.001) were associated with higher CES-D scores. Conclusion: The study findings revealed significant differences between the depressed and normal-mood groups in terms of stress coping skills and resilience, suggesting the need for promoting stress coping strategies and resilience to lower depression-related problems among college students.
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Kim, Suhee,Lee, Sanghyuk,Lee, Hye Jin Elsevier 2018 Sensors and actuators. B Chemical Vol.273 No.-
<P><B>Abstract</B></P> <P>A Gold nanorod (NR) enhanced surface sandwich assay utilizing a novel pair of aptamers for the attomolar concentration of norovirus (NoV) capsid protein was developed in conjunction with surface plasmon resonance (SPR). A total of four different DNA aptamer sequences (aptamer I-IV) known to be specific for the NoV protein were examined using SPR for individual binding strength with the NoV protein and for the formation of surface sandwich with the NoV protein, meaning that the chosen aptamer pair possesses different binding epitope towards the NoV protein. One of the aptamer (aptamer II) sequences with the strongest binding constant was covalently tethered onto a chemically modified thin gold chip surface, while the other aptamer I was used for tethering onto the Au NR surface. The surface sandwich complex was formed via the sequential adsorption of NoV capsid protein and Au NR coated aptamer I onto the aptamer II surface. As low as a 70 aM concentration of the NoV protein in buffer solution could be detected, which is 10<SUP>5</SUP> times better than that of using the aptamer-aptamer sandwich platform without any gold NR particles. As a demonstration, the aptamer-NR coated aptamer sandwich assay was applied to analyze NoV capsid protein concentrations spiked in human serum solutions.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Highly sensitive and selective surface sandwich bioassays for norovirus (NoV) capsid protein. </LI> <LI> A pair of DNA aptamer bioreceptors for enhancing the selectivity of NoV capsid protein sensing. </LI> <LI> Gold nanorod-DNA aptamer conjugates to improve the sensitivity for NoV capsid protein sensing. </LI> <LI> Direct analysis of NoV capsid protein concentrations in undiluted human serum samples. </LI> <LI> A lowest detectable concentration of 50 aM NoV capsid protein. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
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Kim, Suhee,Kim, Hyunwoo,Lee, Soo-Hyeon,Cho, Ilhan,Kang, Seongwoo,Bae, Junwoo,Kim, Woosun,Ahn, Soomin,Choi, Jihye,Kim, Sang-Ki,Do, Yoonjung,Yoo, Jae Gyu,Park, Jinho,Yu, DoHyeon 한국조명·전기설비학회 2017 한국조명·전기설비학회 학술대회논문집 Vol. No.
<P>Acute lymphocytic leukemia (ALL) is uncommon lymphoid malignancy in dogs, and its diagnosis is challenging. A 14-year-old spayed female mixed breed dog was transferred to a veterinary medical teaching hospital for an immediate blood transfusion. The dog showed lethargy, pale mucous membranes, and a weak femoral pulse. Complete blood count revealed non-regenerative anemia and severe leukopenia with thrombocytopenia. ALL was tentatively diagnosed based on the predominance of immature lymphoblasts on blood film examination. For confirmation of lymphoid malignancy, PCR for antigen receptor rearrangement (PARR) on a peripheral blood sample and flow cytometry analysis were performed after blood transfusion. Flow cytometry analysis revealed that lymphocyte subsets were of normal composition, but PARR detected a T-cell malignancy. The dog was diagnosed with ALL and survived 1 wk after diagnosis. In conclusion, after blood transfusion, flow cytometry was not a reliable diagnostic method for an ALL dog, whereas PARR could detect lymphoid malignancy. Our results suggest that PARR should be the first-line diagnostic tool to detect canine lymphoid malignancy after a blood transfusion.</P>
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Kim, Min Je,Jung, A-Ra,Lee, Myeongjae,Kim, Dongjin,Ro, Suhee,Jin, Seon-Mi,Nguyen, Hieu Dinh,Yang, Jeehye,Lee, Kyung-Koo,Lee, Eunji,Kang, Moon Sung,Kim, Hyunjung,Choi, Jong-Ho,Kim, BongSoo,Cho, Jeong H American Chemical Society 2017 ACS APPLIED MATERIALS & INTERFACES Vol.9 No.46
<P>We report high-performance top-gate bottom-contact flexible polymer field-effect transistors (FETs) fabricated by flow-coating diketopyrrolopyrrole (DPP)-based and naphthalene diimide (NDI)-based polymers (P(DPP2DT-T2), P(DPP2DT-TT), P(DPP2DT-DTT), P(NDI2OD-T2), P(NDI2OD-F2T2), and P(NDI2OD-Se2)) as semiconducting channel materials. All of the polymers displayed good FET characteristics with on/off current ratios exceeding 10(7). The highest hole mobility of 1.51 cm(2) V-1 s(-1) and the highest electron mobility of 0.85 cm(2) V-1 s(-1) were obtained from the P(DPP2DT-T2) and P(NDI2OD-Se2) polymer FETs, respectively. The impacts of the polymer structures on the FET performance are well-explained by the interplay between the crystallinity, the tendency of the polymer backbone to adopt an edge-on orientation, and the interconnectivity of polymer fibrils in the film state. Additionally, we demonstrated that all of the flexible polymer-based FETs were highly resistant to tensile stress, with negligible changes in their carrier mobilities and on/off ratios after a bending test. Conclusively, these high-performance, flexible, and durable FETs demonstrate the potential of semiconducting conjugated polymers for use in flexible electronic applications.</P>
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Kim, Eunju,Kim, Seong Bum,Baek, Youl Chang,Kim, Min Seok,Choe, Changyong,Yoo, Jae Gyu,Jung, Younghun,Cho, Ara,Kim, Suhee,Do, Yoon Jung The Korean Society of Veterinary Service 2018 韓國家畜衛生學會誌 Vol.41 No.4
Rumen cannulation is used for nutritional and microbiological research, clinical diagnosis, and rumen component transfaunation. However, the cannulation procedure can affect parameters such as complete blood count findings, serum chemistry, and rumen fluid pH. The objective of this study was to evaluate the health risks related to the rumen cannulation procedure over a 1-month period. We did not identify significant differences in red blood cell numbers or morphologies between pre- and postoperative timepoints. Moreover, no inflammation or infection was detected. Despite the absence of apparent clinical signs after surgery, serum chemistry results revealed changes in blood urea nitrogen levels and the activities of liver enzymes, including aspartate transaminase, lactate dehydrogenase, and creatinine kinase, from postoperative days 1 to 14. Rumen fluid pH, as measured from samples collected via an orogastric tube, was slightly increased after a preoperative fasting period and on postoperative day 1 but decreased thereafter from postoperative day 4, indicating a minor influence of cannulation surgery on ruminal fluid pH. This is the first study to evaluate hematological parameters and rumen pH before and after rumen cannulation surgery in Hanwoo cattle. Further research is required to better elucidate the potential effects of rumen cannulation surgery on animal health.
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KIM, Young-Ok,HONG, Suhee,NAM, Bo-Hye,LEE, Jeong-Ho,KIM, Kyung-Kil,LEE, Sang-Jun Japan Society for Bioscience, Biotechnology, and A 2005 Bioscience, Biotechnology, and Biochemistry Vol.69 No.7
<P>Hepcidin is a cysteine-rich cationic antimicrobial peptide central to iron metabolism. We report a comparative analysis of the sequences, gene organization and expression of two hepcidin genes from olive flounder <I>Paralichthys olivaceus</I>. Both consist of two introns and three exons that encode a prepropeptide (81 amino acids for hepcidin I and 89 amino acids for hepcidin II). A TATA box and several consensus-binding motifs for transcription factors were found upstream of the transcriptional starting site. Hepcidin II was predominantly expressed in the liver and highly inducible under the effect of lipopolysaccharide (LPS), while a large amount of hepcidin I transcripts was detected in various tissues but did not appear to have a significant effect during LPS-stimulation.</P>
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