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Jin, Sang‐,Man,Shin, Jun Seop,Kim, Kang Seok,Gong, Chang‐,Hoon,Park, Su Kyoung,Kim, Jung‐,Sik,Yeom, Su‐,Cheong,Hwang, Eung Soo,Lee, Choon Taek,Kim, Sang‐,Joon,Park, Chung Blackwell Publishing Ltd 2011 Xenotransplantation Vol.18 No.6
<P>Jin S‐M, Shin JS, Kim KS, Gong C‐H, Park SK, Kim J‐S, Yeom S‐C, Hwang ES, Lee CT, Kim S‐J, Park C‐G. Islet isolation from adult designated pathogen‐free pigs: use of the newer bovine nervous tissue–free enzymes and a revised donor selection strategy would improve the islet graft function. Xenotransplantation 2011; 18: 369–379. © 2011 John Wiley & Sons A/S.</P><P><B>Abstract: </B><B> Background: </B> In clinical trials using adult porcine islet products, islets should be isolated from the designated pathogen‐free (DPF) pigs under the current good manufacturing practice (GMP) regulations. Our previous studies suggested that male DPF pigs are better donors than retired breeder pigs and histomorphometrical parameters of donor pancreas predict the porcine islet quality. We aimed to investigate whether the use of the newer bovine nervous tissue–free enzymes and a revised donor selection strategy could improve the islet graft function in the context of islet isolation with DPF pigs.</P><P><B>Methods: </B> Using 30 DPF pigs within a closed herd, we compared the islet yield of porcine islets isolated with Liberase PI (n =<I> </I>11, as a historical control group), Liberase MTF C/T, which is a GMP‐grade enzyme (n<I> </I>=<I> </I>12), and CIzyme collagenase MA/BP protease (n<I> </I>=<I> </I>7). We analyzed the relationship between the diabetes reversal rate of recipient NOD/SCID mice (n<I> </I>=<I> </I>75) and histomorphometric parameters of each donor pancreas as well as donor characteristics.</P><P><B>Results: </B> Proportion of islets larger than 200 μm from the biopsied donor pancreas (P<I> </I>=<I> </I>0.006) better predicted islet yield than age (P<I> </I>=<I> </I>0.760) or body weight (P<I> </I>=<I> </I>0.371) of donor. The proportion of islets larger than 200 μm from the biopsied donor pancreas was not related to the sex of the donor miniature pig (P<I> </I>=<I> </I>0.358). The islet yield obtained with the three enzymes did not differ, even after stratification of the donor with the histomorphometric parameters of the biopsied donor pancreas and the sex of donor. The use of the newer bovine nervous tissue–free enzymes (P<I> </I><<I> </I>0.001), a higher proportion of large islets in donor pancreas (P<I> </I>=<I> </I>0.006), and a male sex of the donor (P<I> = </I>0.025) were independent predictors of earlier diabetes reversal.</P><P><B>Conclusions: </B> Use of the newer bovine nervous tissue–free enzymes including a GMP‐grade enzyme resulted in better islet quality than that of islet isolated using Liberase PI. To obtain high‐quality islet from DPF pigs, the donor should be male pig and histomorphometrical parameters from donor pancreas should be considered.</P>
순차적인 마이크로 몰딩 방법을 이용한 다중 분획 입자의 제조
염수진(Su-Jin Yeom),강성민(Sung-Min Kang),김종민(Jongmin Kim),남진오(Jin-Oh Nam),엄나예(Naye Eom),이소희(Sohui Lee),이창수(Chang-Soo Lee) 한국고분자학회 2016 폴리머 Vol.40 No.3
본 연구는 순차적인 마이크로 몰딩 방법을 이용한 고분자 다중 분획 입자 제조 방법에 관한 것이다. 다중 분획 입자는 poly(dimethylsiloxane)(PDMS) 마이크로 몰드 내로 단량체 혼합물의 주입, 휘발성 용매의 증발, 광중합으로 이루어지는 일련의 과정을 통해 제조한다. 이때, 단량체 혼합물에 포함된 휘발성 용매의 농도에 따라, 다중 분획입자를 구성하는 각 구획의 부피분율을 제어하는 것이 가능하다. 또한 단량체 혼합물의 농도 및 다중 분획 입자 제조공정의 반복횟수에 따라, 단일 입자, 이중 입자, 삼중 입자를 선택적으로 제조가 가능함을 보여준다. 제조한 각각의 다중 분획 입자에 형광염료를 함입하여 특정한 정보를 인코딩한 바코드 입자로써의 활용 가능성을 증명한다. 본 연구에서 제안하는 다중 분획 입자를 제조하기 위한 순차적인 마이크로 몰딩 방법은 쉽고, 빠르며, 고가의 장비를 필요로 하지 않는 장점을 가지며, 제조된 다중 분획 입자는 약물 저장 및 전달 소재, 바이오 센서, 그리고 선택적인 흡착-탈착을 유도할 수 있는 고감도 기능성 재료로서 응용이 가능할 것으로 기대한다. In this paper, we present a fabrication method of polymeric multicompartment particles via a sequential micromolding process composed of injection of photocurable solution, evaporation of volatile solvent, and photo-polymerization. Depending on the concentration of volatile solvent in photocurable solution, the volume fraction of the multicompartment particles can be controlled. Also, the repetition of the sequential micromolding process with controlled composition of photocurable solution provides controlling the number of compartment in the particles. Based on this principle, we can fabricate single particles, Janus particles and triblock particles with desired fraction of the compartment. In addition, the multicompartment particles are able to be applicable for barcode particles embedding fluorescent dyes at each compartment. The barcode particles encode information about their specific compositions and enable simple identification. These sequential micromolding method for multicompartment particles has several advantages including easy, fast, and cost effective process. We envision that the multicompartment particles have various applications such as drug storage, delivery supporters, biosensors, and advanced materials for inducing highly selective adsorption-desorption.
Lee Yongseop,Cho Yun Suk,Sohn Yu Jin,Hyun Jong Hoon,Ahn Sang Min,Lee Woon Ji,Kim Jung Ho,Seong Hye,Kim Junhyoung,Jeong Su Jin,Ku Nam Su,Yeom Joon Sup,Ahn Jin Young,Choi Jun Yong 대한감염학회 2020 Infection and Chemotherapy Vol.52 No.4
Background: The aim of this study was to describe the clinical and microbiological characteristics of infective arthritis and to analyze risk factors for Gram-negative bacterial infections that cause infective arthritis. Materials and Methods: Patients admitted between 2009 - 2018 with infective arthritis in a single-tertiary hospital were evaluated retrospectively. Results: A total of 181 patients were enrolled in this study. Of them, 135 were native joint infection patients and 46 were prosthetic joint infection patients. The most common site of infective arthritis was the knee (63.6%), followed by the shoulder (17.7%), and the hip (9.9%). The most frequently identified microorganisms were Staphylococcus aureus (51.1%), followed by Streptococci sp. (21.1%), Enterobacteriaceae (8.4%), and coagulase-negative-Staphylococci (CNS; 8.4%). Infections due to Gram-negative bacteria and fungi made up 13.7% and 3.2% of all cases, respectively. Additionally, 20% and 4.2% of the cases involved methicillin-resistant S. aureus (MRSA) and MRCNS. We found that bacteriuria, infective arthritis in the hip, and steroid use at admission are independent risk factors for Gram-negative bacterial infections. Conclusion: Infective arthritis with methicillin-resistant microorganisms reached up to about 25% in a single-tertiary hospital in Korea. In case of suspected urinary tract infection, infective arthritis of the hip joint, or steroid use at admission time among infective arthritis patients, empirical treatment covering Gram-negative microorganisms can be considered.
Yeom, Soo-Jin,Kim, Nam-Hee,Park, Chang-Su,Oh, Deok-Kun American Society for Microbiology 2009 Applied and environmental microbiology Vol.75 No.21
<B>ABSTRACT</B><P>Two enzymes, l-arabinose isomerase and mannose-6-phosphate isomerase, from <I>Geobacillus thermodenitrificans</I> produced 118 g/liter l-ribose from 500 g/liter l-arabinose at pH 7.0, 70°C, and 1 mM Co<SUP>2+</SUP> for 3 h, with a conversion yield of 23.6% and a volumetric productivity of 39.3 g liter<SUP>−1</SUP> h<SUP>−1</SUP>.</P>
Yeom, Soo-Jin,Kim, Yeong-Su,Oh, Deok-Kun American Society for Microbiology 2013 Applied and environmental microbiology Vol.79 No.3
<B>ABSTRACT</B><P>Phosphosugar isomerases can catalyze the isomerization of not only phosphosugar but also of monosaccharides, suggesting that the phosphosugar isomerases can be used as sugar isomerases that do not exist in nature. Determination of active-site residues of phosphosugar isomerases, including ribose-5-phosphate isomerase fromClostridium difficile(CDRPI), mannose-6-phosphate isomerase fromBacillus subtilis(BSMPI), and glucose-6-phosphate isomerase fromPyrococcus furiosus(PFGPI), was accomplished by docking of monosaccharides onto the structure models of the isomerases. The determinant residues, including Arg133 of CDRPI, Arg192 of BSMPI, and Thr85 of PFGPI, were subjected to alanine substitutions and found to act as phosphate-binding sites. R133D of CDRPI, R192 of BSMPI, and T85Q of PFGPI displayed the highest catalytic efficiencies for monosaccharides at each position. These residues exhibited 1.8-, 3.5-, and 4.9-fold higher catalytic efficiencies, respectively, for the monosaccharides than the wild-type enzyme. However, the activities of these 3 variant enzymes for phosphosugars as the original substrates disappeared. Thus, R133D of CDRPI, R192 of BSMPI, and T85Q of PFGPI are no longer phosphosugar isomerases; instead, they are changed to ad-ribose isomerase, anl-ribose isomerase, and anl-talose isomerase, respectively. In this study, we used substrate-tailored optimization to develop novel sugar isomerases which are not found in nature based on phosphosugar isomerases.</P>
Supramolecular Hydrogels for Long-Term Bioengineered Stem Cell Therapy
Yeom, Junseok,Kim, Su Jin,Jung, Hyuntae,Namkoong, Hong,Yang, Jeonga,Hwang, Byung Woo,Oh, Kyunghoon,Kim, Kimoon,Sung, Young Chul,Hahn, Sei Kwang Wiley (John WileySons) 2015 Advanced healthcare materials Vol.4 No.2
<P>Synthetic hydrogels have been extensively investigated as artificial extracellular matrices (ECMs) for tissue engineering in vitro and in vivo. Crucial challenges for such hydrogels are sustaining long-term cytocompatible encapsulation and providing appropriate cues at the right place and time for spatio-temporal control of the cells. Here, in situ supramolecularly assembled and modularly modified hydrogels for long-term engineered mesenchymal stem cell (eMSC) therapy are reported using cucurbit[6]uril-conjugated hyaluronic acid (CB[6]-HA), diaminohexane conjugated HA (DAH-HA), and drug-conjugated CB[6] (drug-CB[6]). The eMSCs producing enhanced green fluorescence protein (EGFP) remain alive and emit the fluorescence within CB[6]/DAH-HA hydrogels in mice for more than 60 d. Furthermore, the long-term expression of mutant interleukin-12 (IL-12M) by eMSCs within the supramolecular hydrogels results in effective inhibition of tumor growth with a significantly enhanced survival rate. Taken together, these findings confirm the feasibility of supramolecular HA hydrogels as 3D artificial ECMs for cell therapies and tissue engineering applications.</P>
Yeom, Soo-Jin,Seo, Eun-Sun,Kim, Bi-Na,Kim, Yeong-Su,Oh, Deok-Kun American Society for Microbiology 2011 Applied and environmental microbiology Vol.77 No.3
<B>ABSTRACT</B><P>An uncharacterized gene from<I>Thermus thermophilus</I>, thought to encode a mannose-6-phosphate isomerase, was cloned and expressed in<I>Escherichia coli</I>. The maximal activity of the recombinant enzyme forl-ribulose isomerization was observed at pH 7.0 and 75°C in the presence of 0.5 mM Cu<SUP>2+</SUP>. Among all of the pentoses and hexoses evaluated, the enzyme exhibited the highest activity for the conversion ofl-ribulose tol-ribose, a potential starting material for manyl-nucleoside-based pharmaceutical compounds. The active-site residues, predicted according to a homology-based model, were separately replaced with Ala. The residue at position 142 was correlated with an increase inl-ribulose isomerization activity. The R142N mutant showed the highest activity among mutants modified with Ala, Glu, Tyr, Lys, Asn, or Gln. The specific activity and catalytic efficiency (<I>k</I>cat/<I>Km</I>) forl-ribulose using the R142N mutant were 1.4- and 1.6-fold higher than those of the wild-type enzyme, respectively. The<I>k</I>cat/<I>Km</I>of the R142N mutant was 3.8-fold higher than that of<I>Geobacillus thermodenitrificans</I>mannose-6-phosphate isomerase, which exhibited the highest activity to date for the previously reported<I>k</I>cat/<I>Km</I>. The R142N mutant enzyme produced 213 g/literl-ribose from 300 g/literl-ribulose for 2 h, with a volumetric productivity of 107 g liter<SUP>−1</SUP>h<SUP>−1</SUP>, which was 1.5-fold higher than that of the wild-type enzyme.</P>