http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Effect of Annurca Apple Polyphenols on Human HaCaT Keratinocytes Proliferation
Stefania D’Angelo,Raffaele La Porta,Maria Napolitano,Patrizia Galletti,Lucio Quagliuolo,Mariarosaria Boccellino 한국식품영양과학회 2012 Journal of medicinal food Vol.15 No.11
Polyphenols have been demonstrated to have clear antioxidant activities in vitro. However, in complex biological systems, they exhibit additional properties, which are yet poorly understood. The apple is among the most consumed fruits worldwide, and several studies suggest that apple polyphenols could play a role in the prevention of degenerative diseases. The present study aimed at evaluating the Annurca apple polyphenol extract (APE) effects both proliferation and apoptosis on HaCaT cells. The data indicate that apple polyphenolic compounds had significant antiproliferative action on HaCaT cells. The fluorescence-activated cell-sorting analysis showed that APE induced cell apoptosis in a dose-dependent manner. Moreover, apple polyphenols induced apoptosis in epithelial cells by triggering a death receptor-associated extrinsic pathway p53-independent. APE was also capable of inducing morphological changes as evidenced by nuclear condensation. The cellular, morphological, and molecular data unequivocally demonstrated that induction of cellular apoptosis was mainly responsible for the previously observed antiproliferation-induced APE on HaCaT keratinocytes. Our experimental results suggest that apple polyphenols are a promising source from which a natural-based topical agent could be developed for skin diseases treatment.
Protective Effect of Polyphenols from Glycyrrhiza glabra Against Oxidative Stress in Caco-2 Cells
Stefania D'Angelo,Alessandra Morana,Anna Salvatore,Vincenzo Zappia,Patrizia Galletti 한국식품영양과학회 2009 Journal of medicinal food Vol.12 No.6
In the present article, we have investigated the antioxidant properties of methanolic liquorice polyphenol extracts (LPEs). Polyphenol extraction was performed with 60% and 100% methanol. Analysis of LPEs by thin-layer chromatography revealed that a higher amount of polyphenols was recovered by extraction with 60% methanol. Antioxidant activity measurement of the reducing power, scavenging effect on 2,2′-diphenyl-1-picrylhydrazyl free radical, and hydrogen peroxide scavenging capability have been taken as the parameters for assessment of antioxidant potential of LPEs. Results have been compared with both natural and synthetic antioxidants. All experimental data have indicated that LPEs possess strong antioxidant power proportional to their o-diphenolic and total polyphenolic content, independently from the assay used. Therefore, the LPEs antioxidant property was examined against the cytotoxic effects of reactive oxygen species in human colon carcinoma cells. Pretreatment of Caco-2 cells with liquorice polyphenolic extracts provided a remarkable protection against oxidative damage induced by H2O2. The highest oxidative stress protection (72% of cell vitality) was measured in cells pretreated with 0.54mM polyphenols. This effect seems to be associated to the antioxidant activity of liquorice polyphenolic compounds. Our data suggest that polyphenols from Glycyrrhiza glabra could exert a beneficial action in the prevention of intestinal pathologies related to production of reactive oxygen species.