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- 저자 : Sonja Djilas (검색결과 2 건)
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Jasna Canadanovic-Brunet,Gordana Cetkovic,Sonja Djilas,Vesna Tumbas,Gordana Bogdanovi?,Anamarija Mandi?,Sinisa Markov,Dragoljub Cvetkovi?,Vladimir Canadanovi? 한국식품영양과학회 2008 Journal of medicinal food Vol.11 No.1
The aromatic herb Melissa officinalisL. can be used as an easily accessible source of natural antioxidants andas a possible food supplement and as a phytochemical. Radical scavenging, antibacterial, and antiproliferative activities of pe-troleum ether, chloroform, ethyl acetate, n-butanol, and water extracts of M. officinalis L. extracts were investigated. The re-sults of antioxidative activity, obtained by electron spin resonance spectroscopy, confirmed that investigated extracts sup-pressed the formation of 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl, and lipid peroxyl radicals in all investigated systemsin a dose-dependent manner. The maximum DPPH and hydroxyl radical scavenging activities (SADPPH. SAOH . 100%)were achieved in the presence of n-butanol extract at concentrations of 0.4 mg/mL and 0.5 mg/mL, respectively. The highestlipid peroxyl scavenging activity (93.20%) was observed at a higher concentration (5 mg/mL) ofn-butanol extract in the lipidperoxidation system. The most effective antibacterial activities were expressed by petroleum ether and ethyl acetate extractson Sarcina lutea. Chloroform extract showed the strongest antiproliferative effect with 50% inhibitory concentration valuesof 0.09 mg/mL and 0.10 mg/mL for HeLa and MCF-7 cell lines, respectively. The present study demonstrated the high phe-nolic content and radical scavenging, antibacterial, and antiproliferative activities of extracts of M. officinalisL. originatingfrom Serbia.
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Dragana D. Četojević-Simin,Jasna M. C ˇ anadanovic´-Brunet,Gordana M. Bogdanovic,Sonja M. Djilas,Gordana S. C ´ etkovic,Vesna T. Tumbas,Bratislav T. Stojiljkovic 한국식품영양과학회 2010 Journal of medicinal food Vol.13 No.2
In this study we investigated antioxidative and antiproliferative activity of different horsetail (Equisetum arvense L.) extracts. The antioxidative activity was measured by the electron spin resonance (ESR) spectroscopy–spin trapping method. The influence of different horsetail extracts during lipid peroxidation of (1) sunflower oil induced by the lipophilic azo-initiator 4,4'-azobis(4-cyanovaleric acid) and (2) soybean phosphatidylcholine liposomes induced by the hydrophilic azo-initiator 2,2'-azobis(2-amidinopropane) dihydrochloride was studied. Antiproliferative activity was measured using the sulforhodamine B colorimetric assay on the human cancer cell lines HeLa, HT-29, and MCF7. The results of ESR analysis confirmed that the extracts investigated suppressed the formation of lipid peroxyl radicals in both systems investigated in a dose-dependent manner. The results indicate that n-butanol, methanol, ethyl acetate, and water extracts had significant peroxyl radical scavenging activity. Extracts inhibited cell growth that was dependent on cell line, type of extract, and extract concentration. Ethyl acetate extract exhibited the most prominent antiproliferative effect, without inducing any cell growth stimulation on human tumor cell lines. The results obtained suggest that the horsetail extracts could be used as an easily accessible source of natural antioxidants and as potential phytochemicals.
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