식중독균 항원(Salmonella typhimurium)에 의한 계란항체(IgY) 생산성과 항체특성 및 항균효과

백경연,손규목,배만종,백반석,한준표 경산대학교 생명자원개발연구소 1999 생명자원과 산업 Vol.3 No.-

본 연구는 식중독균을 항원으로 하여 산란계에 면역시킨 후 계란 항체(IgY)의 생산 가능성을 검토하고, 그 항체의 특성과 항균성을 조사하고자 실시하였다. 그 방법으로는 항원으로 사용된 Salmonella typhimurium(ATCC 14028) 등을 incomplete Freund's adjuvant와 동량으로 혼합하여 면역시킨 후 10주간 생산된 계란을 수집하여, 계란항체(IgY)의 분리 및 식중독균 항원에 대한 항체의 특성, 항균성, 생화학적 특성을 측정하였다. S. typhimurium 항원에 면역된 산란계의 계란항체(IgY)생산 및 증가를 측정한 결과, 산란계가 이들 항원에 면역반응을 일으켜 2주 후 혈청과 난황에서 각각 normal군에 비하여 뚜렷한 항체가 생성능을 보였으며, 혈청내의 IgG생성에 비하여 계란항체(IgY)의 생성이 다소 낮은 것으로 나타났다. 혈청 및 난황내의 단백질 함량은 normol군에 비하여 약간 높은 경향을 나타내어 산란계의 혈청 및 난황내의 단백질이 이들 항원에 의하여 증가하는 것으로 나타났다. DEAE-Sephacel column을 통과한 fraction은 두 개의 peak를 나타내었다. 각 fraction의 IgY농도를 ELISA법으로 확인한 결과 두 번째 peak에서 대부분 측정되었다. 분리 정제된 IgY의 분자량을 SDS-polyacrylamide 전기영동법으로 측정한 결과, heavy chain 72∼75KD, light chain 30∼40KD정도였다. Anti-S. typhimurium 항체의 온도에 대한 활성은 60℃에서 30분 가열시 91.5%, 65℃에서 15분 가열시 각각 73.2%의 활성도를 나타내었으며, 80℃에서 15분간 가열시는 거의 활성이 없는 것으로 나타났다. pH에 대한 안정성은 pH 7부터 pH 4까지는각각 98.7%의 활성도를 유지하다가 pH 3부터 급격히 감소하여, pH 2에서는 9.6%로 거의 활성도를 나타내지 않았다. 항균활성을 응집가, 생균수, disc diffusion susceptibility test 등으로 측정한 결과 anti-S. typhimurium 항체의 항균활성도는 매우 미약하게 나타났다. 이상의 결과에서, 본 실험에서 사용된 IgY는 항원에 대한 특이성 및 생화학적 특성 등을 고려해 볼 때 앞으로 식중독균 제거 및 예방 치료뿐만 아니라 식품산업 소재, 각종 질병예방을 위한 의약품 소재, 연구용 kit와 진단용 kit 및 축산분야 등 여러 분야에 응용될 수 있으리라 기대된다. This study was carried out to investigated to get a industrial information from discussion about productive a possibility if IgY antibody, antimicrobial activity and properties of IgY antibody in egg yolk. Immunoglobulin Y in egg yolk and immunoglobulin G in serum were obtained from gens which were immunized with Salmonella typhimurium(ATCC14028). These antibody titers were evaluated in serum and egg yolk by ELISA. After the initial immunization the anti-Salmonella typhimurium IgY antibody level gradually were decreased from firth week to tenth week. On the other hand, the antibody level in the serum were increased from the first week, reaching its peak in the sixth week. The residual antibody activities of IgY were 91.5% after heating for 30min at 60℃. At the same time, were 73.2% after heating 15min at 65℃ and decreased vapidly at 70℃ and little antibody activity was left after heating for 15min at 80℃. When the prepared IgY was stable from pH 7 to 4 and remained to 69.8% at pH 3, respectively.

단백체를 이용한 애기장대 Cytokinin 유도 단백질의 분석

양영실,차준영,네티 엘마와티,정민희,배동원,이창원,손대영 Plant molecular biology and biotechnology research 2005 Plant molecular biology and biotechnology research Vol.2005 No.

Cytokinins are essential plant hormones that play crucial roles in various aspects of plant growth and development. To better understand the molecular mechanisms of cytokinin action, we identified cytokinin related proteins by a proteomic approach. Proteins extracted from control and trans-zeatin treated Arabidopsis seedlings were separated and analyzed by two dimensional gel analysis. Differentially expressed protein spots were identified with peptide mass fingerprinting based on matrix assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry and database searching. We obtained ten up-regulated and one down-regulated proteins upon t-zeatin treatment. The expression of the following proteins was induced; pollen allergen like protein, L-ascorbate peroxidase, tetrapyrrole methylase family protein, SGT1 protein homolog, disease resistance related protein, maternal embryogenesis control protein, paxneb related protein, gluthathione S-transferase and IAA amino acid hydrolase homolog.

FEM을 이용한 GMA 용접공정의 비드형상 모델링

박창언,김일수,장영호,정영재,손준식,배수연 木浦大學校 工業技術硏究所 1997 工業技術硏究誌 Vol.7 No.-

Over the last few year, there has been a growing interest in quantitative representation of heat transfer phenomena in weld pools in order to relate the processing conditions to the quality of the weldment produced and to use this information for the optimisation and robotization of the welding process. Normally, a theoretical model offers a powerful alternative to check out the physical concepts of the welding process and to calculate the effects of varying any of parameters. To solve this problem, a transient 2D(two-dimensional) heat conduction model were developed for determining weld bead geometry and temperature distribution for the GMA welding process. The equation was solved using a general thermofluid-mechanics computer program, PHOENICS code, which is based on the SIMPLE algorithm. The simulation results showed that the calculated bead geometry from the developed model reasonablely agrees with the experiment results.

Fabrication of NiO-Y:BaZrO₃ Composite Anode for Thin Film-Protonic Ceramic Fuel Cells using Tape-Casting

Bae, Kiho,Noh, Ho-Sung,Jang, Dong Young,Kim, Manjin,Kim, Hyun Joong,Hong, Jongsup,Lee, Jong-Ho,Kim, Byung-Kook,Son, Ji-Won,Shim, Joon Hyung The Korean Ceramic Society 2015 한국세라믹학회지 Vol.52 No.5

Optimization of the fabrication process of NiO-yttrium doped barium zirconate (BZY) composite anode substrates using tape-casting for high performance thin-film protonic ceramic fuel cells (PCFCs) is investigated. The anode substrate is composed of a tens of microns-thick anode functional layer laminated over a porous anode substrate. The macro-pore structure of the anode support is induced by micron-scale polymethyl methacrylate (PMMA) pore formers. Thermal gravity analysis (TGA) and a dilatometer are used to determine the polymeric additive burn-out and sintering temperatures. Crystallinity and microstructure of the tape-cast NiO-BZY anode are analyzed after the sintering.

High-Performance Protonic Ceramic Fuel Cells with 1 µm Thick Y:Ba(Ce, Zr)O₃ Electrolytes

Bae, Kiho,Kim, Dong Hwan,Choi, Hyung Jong,Son, Ji-Won,Shim, Joon Hyung Wiley (John WileySons) 2018 ADVANCED ENERGY MATERIALS Vol.8 No.25

High-Performance Protonic Ceramic Fuel Cells with Thin-Film Yttrium-Doped Barium Cerate–Zirconate Electrolytes on Compositionally Gradient Anodes

Bae, Kiho,Lee, Sewook,Jang, Dong Young,Kim, Hyun Joong,Lee, Hunhyeong,Shin, Dongwook,Son, Ji-Won,Shim, Joon Hyung American Chemical Society 2016 ACS APPLIED MATERIALS & INTERFACES Vol.8 No.14

<P>In this study, we used a compositionally gradient anode functional layer (AFL) consisting of Ni-BaCe0.5Zr0.35Y0.15O3-delta (BCZY) with increasing BCZY contents toward the electrolyte -anode interface for high-performance protonic ceramic fuel cells. It is identified that conventional homogeneous AFLs fail to stably accommodate a thin film of BCZY electrolyte. In contrast, a dense 2 mu m thick BCZY electrolyte was successfully deposited onto the proposed gradient AFL with improved adhesion. A fuel cell containing this thin electrolyte showed a promising maximum peak power density of 635 mW cm(-2) at 600 degrees C, with an open-circuit voltage of over 1 V. Impedance analysis confirmed that minimizing the electrolyte thickness is essential for achieving a high power output, suggesting that the anode structure is important in stably accommodating thin electrolytes.</P>

Engineering ferromagnetic lines in graphene by local functionalization using AFM lithography

Bae Ho Park,Ik-Su Byun,Danil W. Boukhvalov,Duk Hyun Lee,Wondong Kim,Jaeyoon Baik,Hyun-Joon Shin,Young-Woo Son 한국자기학회 2021 한국자기학회 학술연구발표회 논문개요집 Vol.31 No.1

Monolayer graphene with sp²-carbon-atom network is a promising platform for next-generation spintronic devices due to its high carrier mobility and long spin relaxation length. For implementation of practical and high-density graphene-based spintronic devices, we need to define nanoscale areas with ferromagnetic properties on graphene. Up to now, conventional ferromagnetic metal electrodes accompanied by barrier insulators have been used for injection and detection of polarized spins in graphene-based spintronic devices. If graphene-based materials show ferromagnetic behaviors, they will become ideal candidates for spin injectors and detectors, because they structurally, chemically, and electrically match well with pristine graphene. In this presentation, I will report on local magnetic characteristics of nanoscale graphene oxidized and hydrogenated by atomic force microscope (AFM) lithography without conventional sources of surface contamination and chemical agents. By using AFM lithography, we can selectively control functional groups and their coverages on the nanoscale at the surface of graphene. By performing magnetic force microscope (MFM) measurement, we can clearly distinguish local magnetic signal of selectively oxidized or hydrogenated graphene from that of surrounding pristine graphene which does not produce ferromagnetic signal. The nanoscale oxidized and hydrogenated graphene show experimental evidences for room-temperature ferromagnetism. From x-ray magnetic circular dichroism photoemission electron microscope (XMCD-PEEM) measurement, we also identified remarkable asymmetry in carbon K edge XMCD spectra, which strongly indicates that the observed ferromagnetic order in functionalized graphene layers is intrinsic.

통합재난관리체계 구축 및 활성화를 위한 소방공무원 의식조사 연구

Bae, Young-Son,Koo, Won-Hoi,Shin, Ho-Joon,Baek, Min-Ho 한국재난정보학회 2014 한국재난정보학회 논문집 Vol.10 No.1

본 논문에서는 국내 재난관리체계를 검토하여 문제점을 알아보고 재난현장 일선에 있는 소방공무원을 대상으로 통합재난관리체계에 대한 의식조사를 실시하여 발전방안을 모색하였다. 이를 바탕으로 통합재난관리체계 구축 및 활성화를 위한 방안을 제시하였으며 결론은 다음과 같다. 통합재난관리체계 구축 및 활성화를 위해서는항목별로 다음과 같은 대책이마련되어야 한다. 조직적인 측면으로는 기관별 협조체계가 잘 이루어져야 하며 이에 따른 정보 공유 및 전달이 원활해야 한다. 법 제도적인 측면으로는 기관별 특성을 고려하되 협조상의 혼선이 없도록 개선이 요구되고 기관별 협업을 통한 공동작전계획 수립이 실시되어야 한다. 또한 시스템적 측면으로는 급변하는 재난에 대응하기 위해 장비의 지속적인 유지관리 및 업데이트를 실시해야 하고 조직, 법 제도, 시스템 등을 체계적이고 균형적으로 유지해야한다. 그리고 선진국 통합재난관리체계 장단점을 세부적으로 파악하여 국내에 특성에 맞게 적용할 수 있도록 세부적인 대책이 마련하고 유관기관의 통합상황관리체계 구축, 효율적인 통합재난관리체계 구축을 통한 상황실 일원화 등 세부적인 대책이 수립되어야 한다. In this study, the problems in the domestic disaster management system were identified through the examination of the system, relevant implications were drawn through the present status of domestic integrated disaster management system, and the consciousness survey for the integrated disaster management system targeting fire-fighting officers in the front line of the disaster was carried out to identify the measures to improve the system. Based on the findings, the measures to establish and revitalize the integrated disaster management system was presented, and the contents of the measures are as follows. In order to establish and revitalize the integrated disaster management system, the following measures should be prepared for each item. In the organizational aspect, the coordination system between government agencies should be established properly and the relevant information should he shared and delivered smoothly. In the legal and institutional aspect, the improvement is required to prevent any miscommunications in the coordination and joint operation plan through the collaboration between government agencies should be established in consideration of characteristics of each government agency. Also, in the aspect of system, the equipment should be maintained and updated continuously in order to respond to rapidly changing disasters and the organization, laws, institutions and system should be improved systematically and harmoniously. And, the detailed measures appropriate for the characteristics of domestic disasters should be prepared through the examination of advantages and disadvantages of integrated disaster management system in advanced countries in details and the detailed plans for the establishment of integrated situation management system in relevant organizations and the unification of situation room through the establishment of efficient integrated management system should be established.

제주도 연안해역의 해황 및 기상이 어획생산량의 변동에 미치는 영향

손태준,박원배,노홍길,양성기 제주대학교 1992 논문집 Vol.34 No.-

. Long-term time series data of water of water temperature and surface meteorological elements are used to investigate effects on the fishery production in the neghboring seas of Cheju island. Long-term(more then 12 months) and the seasonal variations between the sea surface temperature and the air temperature are highly correlated excepting the phase difference by the unusual cold period. Also the ocean conditions in the shallow water of Cheju island are easily affected by meteorological conditions and have a large variation in comparison with the often ocean. The sea conditions in the near seas of Cheju island are related to the fishery productions are increased. The fishery productions by the set-net are shown the maximum value in the Fall, radiating from the ocean to the air, and it was shown that the optimum temperature was 19℃, salinity 32.0-32.6 and density 22.6-23.2. The maximum catches of fish were appeared to be related to the times before and after the passing of typhoon or of a cyclone, the sea surface temperature decreased, turbidity was increased and fishery procuction was significantly increased.

열공형과 비열공형 피질하 혈관성 치매에서 위험인자의 차이에 관한 비교 연구

배희준,정지향,유경호,나덕렬,김상윤,최경규,양동원,손의주,이상도,김재우,박경원,김응규,이재홍,박미영,한일우,함동석,최문성,하충건,최성혜,이애영,이병철,한설희 대한치매학회 2003 Dementia and Neurocognitive Disorders Vol.2 No.2

Backgrounds and Objectives: Vascular dementia is a group of dementing disoders arising from various stroke syndrome. Among these. subcortical ischemic vascular dementia (SIVD) is regarded as a relatively distinct clinical entity. However, MRI patterns of SIVD are not homogenous. In some patients, lacunes are dominant, and in others, subcortical white matter changes are. This study was designed to compare risk factor profiles between SIVD with and without multiple lacunes. Methods: We divided 47 subjects (22 males, mean age. 68 years) recruited from VADAPET (Multicenter Trial For Evaluation Of The Changes In the PET Images Of Subcortical Vascular Dementia Patient) study into two groups one with more than 5 lacunes in deep gray matter (lacune group) and the other with 5 or less(non-lacune group) Clinical characteristics and laboratory findings of two groups were compared. Results: Nineteen of 47 patients (40%) belonged to the lacune group. The lacune and non-lacune groups d d not differ in the following variables: age, hypertension, diabetes mellitus, hyperlipidemia heart disease, history of stroke or TIA, history of trauma or major surgery, family history of hypertension stroke, or dementia, age at diagnosis of dementia, body mass index, white blood cell count, ESR, CRP, fibrinogen, hemoglobin A1C, total cholesterol. LDL cholesterol creatinine, proteinuria, glucosuria, and microhematuria. However, male sex, smoking alcohol. hemoglobin, and HDL cholesterol were possibly associated more with lacune group SIVD than with non-lacune group (p<0 1) Multivariate analyses revealed that smoking, hemoglobin, and HDL cholesterol were independent predictors of SIVD with multiple lacunes Conclusion: Our study suggests that SIVD with multiple lacunes may be significantly different in smoking habits hemoglobin, and HDL cholesterol from SIVD without multiple lacunes.