cAMP 농도에 영향을 미치는 수종약물이 파골세포형성에 미치는 영향

소영,고성희,백정화,김관식,정동균 대한구강생물학회 1992 International Journal of Oral Biology Vol.16 No.2

To study the effect of cAMP on the generation of osteoclasts from their precursor cells, the bone marrow cells were isolated from 7 to 9 week-old male mice. The femur and tibia were dissected aseptically and the marrow cavity was flushed with 1 ㎖ of α-minimum essential medium by slow injection. Collected marrow cells were cultured at 1.5-2.0×10^6 cells/well in 24-well plate for 8 days. In experimental group, PGE_2(5×10^-6, 10^-5M), forskolin(10^-5M) or IBMX(10^-4, 10^-5M) were added singly or in combination from the 1st day culture. After cultures, staining for tartrate-resistant acid phosphatase(TRACP)-marker enzyme of osteoclast-was performed according to the modified method of Burstone. The TRACP-positive multinucleated cells(MNC), which have 3 or more nuclei, were counted. The observed results were as follows. 1. In control group. TRACP-positive mononuclear cells were present, but no TRACP-positive multinucleated cells appeared. 2. PGE_2(5×10^-6M) or forskolin(10^-5M) significantly stimulated the formation of TRACP-positive MNC. Moreover, forskolin potentiated the TRACP-positive MNC formation induced by PGE_2 when added simultaneously. 3. IBMX(10^-4, 10^-5M), when added alone, significantly stimulated the formation of TRACP-positive MNC. However, IBMX(10^-5M) added in combination of PGE_2(10^-5M) partially inhibited the TRACP-positive MNC formation induced by PGE_2.

타타늄의 열처리 조건에 따른 동전위 양극분극특성 및 표면 미세구조 변화

주동현,이용렬,정영화,양홍서,박상원,이도재,송호준,박영준 大韓齒科器材學會 2002 대한치과재료학회지 Vol.29 No.2

The corrosion resistance of metallic implants is essential for successful implant osseointegration. Moreover, the implant surface should have appropriate surface roughness for the attachment of the osteoblast and collagen fibrils. When titanium is exposed to air a thermodynamically very stable oxide film is formed at once. This oxide is very thin and yet it protects the metal from corrosion instantaneously. However, the oxide would not be ideal, and it would be supposed that the state of oxide film would be various by the different conditions when it is formed. The objective of this study was to evaluate the electrochemical properties and surface microstructural changes of titanium after passivation treatment or exposure to high heat (in vacuum or in air). ASTM grade 2 commercially pure titanium (Ti) disks of 10㎜ diameter were wet ground and polished with 240 and 600 grit SiC, and then ultrasonically cleaned with distilled water and ethanol. Cleansed as-received samples (As-R group) were passivated with 30% nitric acid solution at 50℃ for 15 minutes to make the Pas group. The Std-V samples were made by treating the Ti disks in vacuum quartz tube (10^-8 Torr) at 530℃ for 40 min for stress relieving, followed by annealing at 700℃ for 90 min, and then slow cooling to room temperature. The Std-A samples undergo identical procedure as the Std-V group except that it is done in air. For 530℃-V samples, Ti disks were heated at 530℃ for 40 minutes and slow cooled to room temperature. The 1000℃-V samples were produced by heating Std-V specimens again at 1000℃ for 2 hr, followed by cooling to room temperature. 530℃-A and 1000℃-A samples were also prepared. Potentiodynamic anodic polarization measurement, SEM, AFM and XRD analysis were performed to evaluate the effects of the various treatments. The difference in the mean values of E_corr between groups showed statistically significant difference (P<0.05), but that of I_corr did not show significance (P>0.05). When Ti surface was passivated there was a shift of corrosion potential (E_corr) in the noble direction and a lower corrosion current density (I_corr) value. Std-A samples showed most noble E_corr value among the tested groups. 530℃-V samples showed more noble E-corr than other tested groups except Std-A group. 1000℃-V samples showed more noble E-corr than those of Std-V sample. In the case of Std-, 530℃- and 1000℃-groups, -A sub-groups showed better corrosion resistant characteristics than -V sub-groups in regard to E-corr and I_corr values. In the SEM observations, the surfaces of the As-R samples showed only scratch line on smooth surface. In Pas samples, thick oxide layer covered the surface under high magnification observation. 530℃-A samples were covered by oxide granules, and Std-A samples had 25~50 nm-thick acicular oxides. The 1000℃-A samples were covered by coarse oxide crystals with a granule diameter of 0.1~0.6 μm having a gross appearance of milky white color, which was identified by XRD as a rutile. On the other hand, 1000℃-V samples showed an Widmansta¨tten structrue of which the orientation of the lattice is formed along crystallographic planes of the parent crystals. On the AFM observation, 530℃-A samples showed RMS roughness of 79.38 nm which is 5 times rougher than those of the As-R samples. Std-A sample had a roughness of 330.47 nm which is about 20-times rougher than that of the As-R sample. 1000℃-A samples were covered by coarse granular oxides and the roughness were about 0.56 μm. Vacuum sub-group samples showed a more smooth surface than air sub-group samples, and the oxide film was more dense. Due to variability of the surface treatment and heat treatment conditions, the process of the oxide formation seems to be affected thereby making the corrosion properties and surface micro-morphology different. However, more various electrochemical analysis together with surface analysis, and an investigation for the effects of those on the osteoblast cell adhesion and calcium phosphate apposition are needed for the improvement of the osseointegration rates of the titanium implants.

미혼 남녀의 결혼관에 대한 연구

김양희,문영소,박정윤 중앙대학교 생활과학연구소 2003 생활과학논집 Vol.17 No.-

While most of adults in the past thought that marriage was compulsory, now marriage is considered not as compulsory but as optional, which is being on a decreasing trend. Accordingly, their intention to marry, time and motive of marriage depend on the individual situation or preference rather than the standardized and uniform way. This study was conducted to analyze the intention of unmarried men and women to marriage more concretely and in depth. The survey of 316 students who took a course of 'Marriage and Family' in Chung-Ang University was conducted with a way of self-description as a method for a qualitative survey to analyze their consciousness to marriage more deeply, classifying into a total of four questions. The findings of the survey were as follows. First, in terms of the intention to marriage, 93.0% of the responders answered "I will marry", indicating the majority of the students were considering marriage positive. In addition to it, in terms of the connection of love and marriage, 61.0% of the responders answered love and marriage were connected with each other, compared to 34.0% who answered seperate. Second, in terms of the motive of marriage, realization of love topped, followed by a valuable thing, emotional stability, providing for their old age, economic stability, independence from their parents and happy married life of their parents. Third, in terms of the reason to choose single, freedom topped, following by concern at change of love, unnecessity of marriage, burden on a new family, negative image to marriage, description and escape from reality, economic responsibility and the cumbersome processes of marriage.

초·중·고등학교 학생, 학부모, 일반교사의 보건교육 요구

윤순녕,김영임,최정명,조희순,김영희,박영남,오경순,이분옥,조선녀,조소영,한선희,하영미 韓國學校保健學會 2004 韓國學校保健學會誌 Vol.17 No.2

Purpose : The purpose of this study was to identify needs of health education of the students, parents, general teachers, and health teacher of the elementary, middle, and high schools. Method : 279 schools of elementary, middle, and high school were sampled nonproportionally during the period from June 28 to July 26, 2004 . The data were collected by the questionnaire from 1,888 students, 1,876 parents, 1,695 general teachers, 279 health teachers. A frequency, % and χ2-test were used to analysis by SAS program. Result : 1. It showed that 98.1% of elementary school students, 92.3% of middle school students, 89.6% of high school students answered that they need health education. The most of students, parents, and general teachers had high interests in health education. 2. In the case of students, main causes of needs of health education was 'to prevent diseases or accidents''. But parents answered that it was 'to build up a healthier behavior'. General teachers answered that it was 'more effective systematic and continuous health education'. 3. Over 80% of students, parents, and general teachers about the question of who qualified person is to teach health education as a regular class responded that health teacher is available. Especially 93% of elementary school students answered like that. 4. The most of students, parents, and general teachers answered that health education in the classroom is favorable 1 to 2 hours per week. Conclusion : As results of the study , the majority of students, parents, general teachers agreed needs of health education. Therefore, on the basis of this study, systematic and continuous health education is necessary. Additionally the establishment of independent health subject is required certainly.

컴퓨터 작업 시 의자 등받이 위치가 근육 활성도에 미치는 영향

김민우,변승진,이경은,정소영,조주영,최원자,최찬양,김경 대구대학교 특수교육재활과학연구소 2011 再活科學硏究 Vol.29 No.1

이 연구는 컴퓨터 작업시 의자 등받이 위치가 근육활성도에 미치는 영향을 알아보기 위해 36명의 근골격에 문제가 없는 성인 남녀가 참가하였으며 대상자들은 등받이가 뒤에 있는 의자와 등받이가 없는 의자, 등받이가 앞에 있는 우리들 의자를 무작위로 배정하고 동일한 컴퓨터 작업을 실시하게 하였다. 실험 중, 표면근전도기를 사용하여 의자에 따른 위등세모근, 머리널판근 그리고 척주세움근의 근활성도 변화를 측정하였다. 이 연구의 결과를 종합해보면 등받이가 앞에 있는 의자는 허리 근육의 부담을 줄이지만 목근육에 부담이 늘어나고 등받이가 뒤에 있는 의자와 없는 의자는 허리근육에는 다소 무리가 가지만 목근육에는 부담이 덜하다는 것을 알 수 있었다. 이러한 결과로 환자의 증상에 다라 컴퓨터 작업을 하는 동안 의자 등받이의 위치를 조절하여 치료에 도움이 될 수 있다고 생각되어진다. Objective : In this study, we checked and observed people's change of body muscle and muscle activity who do computer work on different chairs. We set three kind of chairs which one is a stool and another one is the back is fixed, and the other one is the back is fixed in front of chair. Subject : Accounting for 36 health men and women, we divided these people to 3 groups and put them on the three cases(position 1, position 2, position 3). We measured muscle activity of upper trapezius, Splenius Cervics, Erector Spinae from the groups by using surface electromyogram system. Methods : The standard of the electromyogram was 'reference voluntary contraction', and we carried out 'one way anova' to compare muscle activity of three groups. Results : In position 1, reference voluntary contraction of upper trapezius muscle activity was 123.2821%, Splenius Cervics was 141.7526%, and Erector Spinae was 254.5233%. In position 2, reference voluntary contraction of upper trapezius muscle activity was 132.9395%, Splenius Cervics was 141.7526%, and Erector Spinae was 246.6540%. In position 3, reference voluntary contraction of upper trapezius muscle activity was 190.6487%, Splenius Cervics was 270.2332%, and Erector Spinae was 182.1021%. Both upper trapezius and Splenius Cervics muscle activity of position 3 group was higher than the others groups. In position 1 group, Erector Spinae muscle activity was hight than the other groups. And either was position 2 group's(p<0.05). Conclusion : Therefore the chair which is the back is fixed in front is more comfortable for waist muscle but it's not good for neck. And the other chairs are better in waist muscle but not in neck muscle. So far, when we do a computer work, it's prefer to sit on the group 1&2's chairs to reduce neck muscle's stress and sit on group 3's chair to reduce waist muscle's stress.

초·중·고등학교 학생·학부모·일반교사의 보건교육 영역별 요구도와 보건교육

윤순녕,김영임,최정명,조희순,김영희,박영남,오경순,이분옥,조선녀,조소영,한선희,하영미 韓國學校保健學會 2005 韓國學校保健學會誌 Vol.18 No.1

Purpose: The purpose of this study was to identify the needs of health education in students, their parents and teachers in the elementary, middle and high schools and the current situation of health education class. Method: The subjects of this study were a total of 9450 persons including students, their parents and teachers from 279 schools throughout the country. They were selected through convenient sampling. Data were analyzed through x^(2)­test and ANOVA. Result: Students, their parents and teachers replied that 18 dimensions of health education class (DHEC) are necessary. The four DHEC - healthier life style, sex education, mental health and safety education - showed high educational needs in students, their parents, and teacher. High school students had higher educational need of 'symptom management for daily living' than elementary and middle school students. Students, their parents and teachers in elementary school had higher educational needs of 17 DHEC that those in middle and high school. The percentages of schools with health education class taught by health teachers were 99.2%, 75.5% and 66.0% respectively in elementary, middle and high schools. Health education was given mainly using physical education classes at elementary schools, and creative class hours at middle and high schools. In general, health education took 1-3 hours per week at elementary schools, and less than an hour at middle and high schools. Conclusion: Therefore, based on the results, systematic health education class should begin from elementary school to meet the need of health education in students, their parents and teachers, and further study should be made on the number of hours required and the amount of contents of 18 DHEC.

Diagnostic Performance of Core Needle Biopsy for Characterizing Thyroidectomy Bed Lesions

Jeong So Yeong,Baek Jung Hwan,Chung Sae Rom,Choi Young Jun,Song Dong Eun,Chung Ki-Wook,Kim Won Woong,Lee Jeong Hyun 대한영상의학회 2022 Korean Journal of Radiology Vol.23 No.10

Objective: Thyroidectomy bed lesions frequently show suspicious ultrasound (US) features after thyroid surgery. Fine-needle aspiration (FNA) may not provide definitive pathological information about the lesions. Although core-needle biopsy (CNB) has excellent diagnostic performance in characterizing suspicious thyroid nodules, no published studies have evaluated the performance of CNB specifically for thyroidectomy bed lesions. Therefore, we aimed to evaluate the diagnostic performance and safety of CNB for characterizing thyroidectomy bed lesions. Materials and Methods: A total of 124 thyroidectomy bed lesions in 113 patients (79 female and 34 male; age, 23–85 years) who underwent US-guided CNB between December 2008 and December 2020 were included. We reviewed the US imaging features of the target lesions and the histories of previous biopsies. The pathologic results, diagnostic performance for malignancy, and complications of CNB were analyzed. Results: All samples (100%) obtained by CNB were adequate for pathological analysis. Pathological analysis revealed inconclusive results in two lesions (1.6%). According to the reference standard, 50 lesions were ultimately malignant (40.3%), and 72 were benign (58.1%), excluding the two inconclusive lesions. The performance of CNB for diagnosing malignant thyroidectomy bed lesions in the 122 lesions had a sensitivity of 98.0% (49/50), a specificity of 100% (72/72), positive predictive value of 100% (49/49), and negative predictive value of 98.6% (72/73). Eleven lesions were referred for CNB after prior inconclusive FNA results in thyroidectomy bed lesions, for all of which CNB yielded correct conclusive pathologic diagnoses. According to the pathological analysis of CNB, there were various benign lesions (58.9%, 73/124) besides recurrence, including benign postoperative lesions other than suture granuloma (32.3%, 40/124), suture granuloma (15.3%, 19/124), remnant thyroid tissue (5.6%, 7/124), parathyroid lesions (4%, 5/124), and abscesses (1.6%, 2/124). No major or minor complications were associated with the CNB procedure. Conclusion: US-guided CNB is accurate and safe for characterizing thyroidectomy bed lesions.

Morning Glory Syndrome associated with Autosomal Dominant Alport Syndrome with a Heterozygous COL4A4 Mutation

So Jeong Kim,Jeong Eun Lee,곽현덕,Mi Seon Kang,Seong Ah Yu,Go Hun Seo,오승환,Woo Yeong Chung 대한소아신장학회 2021 Childhood kidney diseases Vol.25 No.2

Morning glory syndrome (MGS) is a rare congenital optic disc anomaly with a characteristic fundal finding with severe visual impairment. It may occur in association with various systemic manifestations, even though most of the reported cases were isolated. A 6-year-old male visited the nephrology clinic with a history of microscopic hematuria and at the age of 12 years, he was diagnosed thin glomerular basement membrane nephropathy by kidney biopsy. After the following years, the patient had progressive deterioration of visual acuity, and diagnosed as MGS. Whole Exome Sequencing of this patient and his mother revealed heterozygous COL4A4 mutations [c.81_86del (p.Ile29_Leu30del)]. It is more reasonable to consider MGS seen in this patient as a coincidental finding of autosomal dominant Alport syndrome. To our knowledge, this case represents the first case report of autosomal dominant Alport syndrome associated with MGS.

Characterization of the Lactiplantibacillus as Potential Probiotics for Alleviating Metabolic Syndrome

So Young PARK,Eun Ji LEE,Nan Kyung KIM,So-Jeong KIM,Sun Jay YOON,Dong Nyoung OH,Sung Jin JEONG,Na Yeong BEEN,Ji Hyeon KIM,Da Yeong KIM,Jin Won CHOI,Won Je JANG,Jong Min LEE 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10

Probiotics consumption has become a necessity in diet to metabolic syndrome. In this study, in vitro effects of anti-metabolic syndrome were confirmed on the probiotic strain isolated from feces. Each of the strain upregulated the mRNA expressions on zo-1, claudin-1 and occludin related to intestinal tight junction in human epithelial colorectal cell. In particular, the lactiplantibacillus treatment groups significantly upregulated the erk1/2 and map expression related to cell proliferation. In addition, the strain showed that upregulation of ppar-α related to fatty acid oxidation and downregulation of glut-2 related to glucose uptake. Based on the results of this study, we are conducting in vivo experiments to verify the efficacy of the probiotics for alleviating metabolic syndrome.

퇴행성 뇌질환에서 뇌 자기공명영상 기반 인공지능 소프트웨어 활용의 현재

So Yeong Jeong,Chong Hyun Suh,Ho Young Park,Hwon Heo,Woo Hyun Shim,Sang Joon Kim 대한영상의학회 2022 대한영상의학회지 Vol.83 No.3

The incidence of neurodegenerative diseases in the older population has increased in recent years. A considerable number of studies have been performed to characterize these diseases. Imaging analysis is an important biomarker for the diagnosis of neurodegenerative disease. Objective and reliable assessment and precise detection are important for the early diagnosis of neurodegenerative diseases. Artificial intelligence (AI) using brain MRI applied to the study of neurodegenerative diseases could promote early diagnosis and optimal decisions for treatment plans. MRI-based AI software have been developed and studied worldwide. Representatively, there are MRI-based volumetry and segmentation software. In this review, we present the development process of brain volumetry analysis software in neurodegenerative diseases, currently used and developed AI software for neurodegenerative disease in the Republic of Korea, probable uses of AI in the future, and AI software limitations.