Aging 조건에 의한 SBA-15의 Morphological Control

천종현,김시재,박동호 인제대학교 2003 仁濟論叢 Vol.18 No.1

중간세공 분자체 SBA-15는 tetraethoxysilane을 실리카원으로 하여 triblock copolymer를 구조유지 시약으로 사용하여 합성된다. 이러한 중간 세공 분자체의 응용성은 역시 결정의 외관상 형태에 의 해 좌우되어진다. 규칙적인 모양의 입자를 합성하는 것은 나노 공간의 SBA-15를 이용한 나노물질을 제조하는데 응용되기 때문에 매우 중요하다. SBA-15의 형태는 숙성과정의 온도와 시간을 바꿔 줌으로써 조절된다. 특히 rod 형태의 입자의 길이는 숙성 반응과 수열반응의 조합에 의해 조절된다. Morphology 변화는 형태학적 재 구조화에 의해 설명될 수 있다. Mesoporous molecular sieve SBA-15 using triblock copolymer as a structure directing agent and tetraethoxysilane as a silicon source has been synthesized. The applicability of mesoporous molecular sieve depends also on the external morphology of crystallites. The synthesis of regular shaped particle is very important due to the application for the preparation of nano materials using the nano space of SBA-15. By varying the aging temperature and duration, the morphology of SBA-15 was controlled Especially, the length of rod-shaped particle was controlled with the combination of reaction condition of aging and hydrothermal reaction. The change of morphology could be attribute to the metamorphic reconstruction

혈액 투석 환자에서 중심정맥 협착에 대한 스텐트 삽입술 : Wallstent Placement

임대승,노상필,이유선,정승현,김보영,이정우,강정아,김정희,이민수,정준용,최시완,정진옥,성인환,이강욱,신영태 충남대학교 의과대학 의학연구소 2002 충남의대잡지 Vol.29 No.1

Stenosis of central vein is a common complication arising after percutaneous subclavian vein catheter insertion performed for temporary vascular access in chronic renal failure patients undergoing hemodialysis. There are several treatment methods for the condition like percutaneous angioplasty(PTA), stent insertion, and surgery, but recent trend is toward PTA and stents. Among the patients diagnosed with chronic renal failure from March 1993 to May 2002 and undergoing hemodialysis through AV fistula, the 14 Patients in whom central vein stenosis arose were selected for the study. A total of 28 percutaneous interventions(5 PTA and 23 stent placement) were performed, and restenosis rate and the time taken till the restenosis in de novo lesions and instant lesions were compared. All 28 cases were operated successfully. The 14 cases that received both anigioplasty and stent placement initially. (de novo lesion : 14 cases), Among the 10 cases with de novo lesion that followed up more than 1 year, 3 cases are currently undergoing hemodialysis without restenosis, and the remaining 7 cases have recurred stenosis with the mean time to restenosis of 10.9 months. In the 7 cases in whom stenosis recurred, 11 interventions were done(instent lesion: 11 cases). 4 of these were using only ballon angioplasty with 100% restenosis rate and the mean time of 3 months until restenosis. The remaining 7 cases were using both balloon angioplasty and stent placement, also with 100% restenosis rate but with the mean time of 12 months until restenosis, which was later than the group receiving only balloon angioplasty. In treating the patients with central vein stenosis, stent placement seems to be more advantageous over PTA in terms of restenosis rate and the mean duration of patency. In the case of instent lesion, inserting the stent for the second time after stenosis recurred lengthened the duration of patency compared to performing balloon angioplasty alone.

[PB-0047] Inhibition of gene expression for anti-nutritional factors and allergen in soybean seeds via RNAi-mediated co-transformation

Si Hyeon Kim(Si Hyeon Kim),Wan Woo Yeom(Wan Woo Yeom),Da Hyeon Ha(Da Hyeon Ha),Hye Jeong Kim(Hye Jeong Kim),Young-Soo Chung(Young-Soo Chung) 한국육종학회 2022 한국육종학회 공동학술발표집 Vol.2022 No.-

[PB-0046] Overexpression of IbMYB1a gene induced anthocyanin accumulation in soybean seeds

Si Hyeon Kim(Si Hyeon Kim),Wan Woo Yeom(Wan Woo Yeom),Hye Jeong Kim(Hye Jeong Kim),Hae Gyun Park(Hae Gyun Park),Hye Min Park(Hye Min Park),Cha Young Kim(Cha Young Kim),Young-Soo Chung(Young-Soo Chung) 한국육종학회 2022 한국육종학회 공동학술발표집 Vol.2022 No.-

EV 사용자의 V2G 참여 유도를 위한 시뮬레이터 개발

김현진(Hyeon-jin Kim),김예랑(Ye-Rang Kim),김현수(Hyeon-Su Kim),김성수(Sung-Soo Kim),이시영(Si-Young Lee),윤아윤(Ah-Yun Yoon) 대한전기학회 2024 대한전기학회 학술대회 논문집 Vol.2024 No.7

일부 성인의 치과위생사의 이미지에 미치는 영향요인

박시현(Si-Hyeon Park),강민이(Min-i Kang),김다현(Da-Hyun Kim),김윤희(Yun-Hee Kim),김태희(Tae-Hee Kim),손아름(A-Reum Son),유송현(Song-Hyeon Yoo),김유린(Yu-Rin Kim) 한국구강보건과학회 2021 한국구강보건과학회지 Vol.9 No.1

Objectives: This study is intended to be used as basic data to enhance the image of dental hygienists by confirming the effect of the importance of dental hygienists on the image of dental hygienists. Methods: Hierarchical regression analysis was performed to determine the effect of dental hygienist importance and dental visit intervals on the dental hygienist image step by step. Results: As factors influencing the dental hygienist image, the younger, the more office workers, the shorter the dental visit interval. the higher the importance of the dental hygienist, the more significant the influence on the dental hygienist image was confirmed. Conclusions: In order to establish a professional image of dental hygienists, we expect continuous publicity that can increase the importance of dental hygienists and an increase in occupational ethics awareness that can increase the professional value of dental hygienists themselves.

Knockdown of acetylcholinesterase 1 increased the motility of honey bee worke

Sang Hyeon Kim,Kyung Mun Kim,Kil Won Kim,Si Hyeok Lee 한국응용곤충학회 2016 한국응용곤충학회 학술대회논문집 Vol.2016 No.04

Acetylcholinesterase 1 (AmAChE1) has low catalytic activity and is abundantly expressed in both neuronal and non-neuronal tissues. In previous experiments, we observed that AmAChE1 is rarely expressed in summer while highly expressed in winter. Through additional experiments, the expression of AmAChE1 was suggested to be associated with brood rearing status. Under the assumption that abnormal suppression of brood rearing activity may result in stressful condition in honey bee social community, it was further suggested that AmAChE1 is likely involved in stress management particularly during winter. We hypothesized that the increased docility usually observed in overwintering bees is likely an outcome of stress management in colony, which is mediated by AmAChE1 expression. To verify this, worker bees expressing abundant AmAChE1 were collected in early winter and injected with Amace1 dsRNA to knockdown Amace1. Then, the behavioral activity of the bees was investigated using the EthoVison video tracking system. Honey bees injected with Amace1 dsRNA showed significantly increased motility, which was strongly correlated with the suppressed expression level of AmAChE1 in the abdomen. No apparent reduced expression of AmAChE1 in the head was observed perhaps due to the limited efficacy of RNA interference in the blood-brain-barrier. Our finding suggests that behavioral activity can be regulated, at least, by AmAChE1 expression level in non-neuronal tissue (i.e., fatbody) perhaps via metabolic alteration.

Expression level of acetylcholinesterase 1 is associated with honey bee brooding status

Young Ho Kim,Ju Hyeon Kim,Kyungmun Kim,Si Hyeock Lee 한국응용곤충학회 2015 한국응용곤충학회 학술대회논문집 Vol.2015 No.10

The acetylcholinesterase 1 (AmAChE1) of the honey bee is known to be abundantly expressed both in the central and peripheral nervous systems. AmAChE1 exists mostly in the soluble form with little catalytic activity and has non-neuronal functions. Our preliminary observation showed that AmAChE1 expression fluctuated between the forages and nurses. A more systematic expression profiling of AmAChE1 over a year cycle on a monthly basis revealed that AmAChE1 was predominantly expressed during the winter months with being moderately expressed during the rainy summer time. However, no significant difference in AmAChE1 expression was noticed between the nurse and forager workers. Interestingly, AmAChE1 expression was inhibited when bees were allowed for brooding by placing overwintering bee hives in strawberry green houses with the supplement of pollen diets whereas it was resumed when the bee hives were removed from the green houses, thereby suppressed brooding. To confirm whether brooding status is a main determining factor for the suppression of AmAChE1 expression, active bee hives were placed in a screen tent, thereby hindering foraging, until brooding was completely suppressed, and then allowed to restore brooding by removing the screen. The AmAChE1 expression in the head was up-regulated when brooding was suppressed whereas its expression was down-regulated when brooding was resumed. These finding demonstrates that AmAChE1 expression in the central nervous system (i.e., head) is related with brooding status of honey bee. To understand the connection between the AmAChE1 expression and other pathways related with brooding, currently in progress are the analyses of head transcriptomes of honey bee workers with or without their brooding suppressed.

Comparative Proteome Analysis of Honeybee Workers with or without Expressing Acetylcholinesterase 1

Young Ho Kim,Ju Hyeon Kim,Kyung Mun Kim,Si Hyeock Lee 한국응용곤충학회 2014 한국응용곤충학회 학술대회논문집 Vol.2014 No.10

Recently, the expression of acetylcholinesterase1 (AChE1) in honeybee worker has been found to be seasonally fluctuated. Seasonal investigation on the AChE1 expression profiles revealed that it is abundantly expressed in January but its expression was completely abolished in February in both head and abdomen. In an attempt to predict the physiological function of seasonally expressed AChE1, proteomic analysis of honeybee worker was conducted using the samples collected in January and February. Total protein samples separately extracted from the head and abdomen of honeybee forager were compared by 2-D electrophoresis (2-DE). More than 2-fold differences in expression patterns between the two different samples were observed in 50 and 85 protein spots in the head and abdomen, respectively. Among them, 20 protein spots showing >17-fold differences in expression between the two different samples of the head were identified by mass spectrometry. Most of the proteins were identified to be the major royal jelly protein (MRJP) families (e.g., MRJP, MRJP2 and MRJP3), which are known to be expressed in nurse bees during brooding season, and their expression was significantly higher in January than in February. This result was unexpected because brooding usually began in the study site apiary during February and the worker bees used for analysis were assumed to be foragers (old workers). Thus, current findings suggest, though speculative, that the workers collected in January may function as nurses despite their old ages in January or that MRJPs may have other not-yet-characterized functions, which is apart from the conventionally known roles. Finally, possible association of MRJPs with AChE1 was discussed.

Pathway profiles based on gene-set enrichment analysis in the honey bee <i>Apis mellifera</i> under brood rearing-suppressed conditions

Kim, Kyungmun,Kim, Ju Hyeon,Kim, Young Ho,Hong, Seong-Eui,Lee, Si Hyeock Elsevier 2018 Genomics Vol.110 No.1

<P><B>Abstract</B></P> <P>Perturbation of normal behaviors in honey bee colonies by any external factor can immediately reduce the colony's capacity for brood rearing, which can eventually lead to colony collapse. To investigate the effects of brood-rearing suppression on the biology of honey bee workers, gene-set enrichment analysis of the transcriptomes of worker bees with or without suppressed brood rearing was performed. When brood rearing was suppressed, pathways associated with both protein degradation and synthesis were simultaneously over-represented in both nurses and foragers, and their overall pathway representation profiles resembled those of normal foragers and nurses, respectively. Thus, obstruction of normal labor induced over-representation in pathways related with reshaping of worker bee physiology, suggesting that transition of labor is physiologically reversible. In addition, some genes associated with the regulation of neuronal excitability, cellular and nutritional stress and aggressiveness were over-expressed under brood rearing suppression perhaps to manage in-hive stress under unfavorable conditions.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Gene-set enrichment analysis of the transcriptomes of <I>Apis mellifera</I> with or without suppressed brood rearing was analyzed. </LI> <LI> Obstruction of normal labor induced over-representation in pathways related with reshaping of worker bee physiology. </LI> <LI> Transition of labor is physiologically reversible. </LI> <LI> Brood rearing suppression appeared to result in reduction of neuronal excitability and aggressiveness in worker bees. </LI> </UL> </P>