Fucosylated Chondroitin Sulfate From Sea Cucumber Improves Glucose Metabolism and Activates Insulin Signaling in the Liver of Insulin-Resistant Mice

Shiwei Hu,Ying-Ying Tian,Yao-Guang Chang,Zhao-Jie Li,Chang-Hu Xue,Yu-Ming Wang 한국식품영양과학회 2014 Journal of medicinal food Vol.17 No.7

This study investigated the effects of fucosylated chondroitin sulfate (CHS) isolated from sea cucumber on glucose metabolism and insulin signaling in the liver of insulin-resistant C57BL/6 mice fed a high-fat, high-sucrose diet (HFSD). Male C57BL/6J mice were randomly assigned into six groups: control; HFSD; 1mg RSG/kg$body weight (RSG); 80 mg CHS/kg$body weight (CHS); 20 mg CHS + 1mg RSG/kg$body weight (20 CHS + RSG); and 80 mg CHS + 1mg RSG/kg$body weight (80 CHS + RSG). Blood glucose, insulin parameters, glucose metabolism-related enzymes activities and insulin-signaling transducers in the liver were analyzed at 19 weeks. Results showed that CHS significantly decreased body weight gain, adipose tissue weight, and fasting blood glucose and serum insulin levels in insulin-resistant mice. Rosiglitazone (RSG) is an effective thiazolidinedione hypoglycemic agent, and CHS synergistically enhanced the effect of RSG. CHS feeding normalized the activities of hexokinase, pyruvate kinase, glycogen phosphorylase, glucose-6-phosphatase, and increased glycogen reserves in the liver. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis showed that CHS promoted the mRNA expression of insulin receptors (IR), insulin receptor substrate 2 (IRS-2), phosphatidylinositol 3 kinase (PI3K), protein kinase B (PKB), and glycogen synthase (GS) in the liver of insulin resistant mice, and inhibited glycogen synthase kinase-3 (GSK-3b) mRNA expression. The results suggested that CHS treatment improved glucose metabolism by modulating metabolic enzymes and promoting the PI3K/PKB/GSK-3b signaling pathway mediated by insulin at the transcriptional level. These results provided strong justification for the development of CHS as a functional food.

A novel feed rate scheduling method with acc-jerk-continuity and round-off error elimination for non-uniform rational B-spline interpolation

Hu Yifei,Jiang Xin,Huo Guanying,Su Cheng,Zhou Shiwei,Wang Bolun,Li Hexiong,Zheng Zhiming 한국CDE학회 2023 Journal of computational design and engineering Vol.10 No.1

Feed rate scheduling is a critical step in computer numerical control machining, as it has a close relationship with machining time and surface quality. It has now become a hot issue in both industry and academia. In this article, we present a novel and complete S-shape-based feed rate scheduling method for three-axis non-uniform rational B-spline (NURBS) tool paths, which can reduce high chord errors and round-off errors, and generate continuous velocity, acceleration, and jerk profile. The proposed feed rate scheduling method consists of three modules: a bidirectional scanning module, a velocity scheduling module, and a round-off error elimination module. The bidirectional scanning module aims to guarantee the continuity of the feed rate at the junctions between successive NURBS blocks, where the chord error, tangential acceleration, and tangential jerk limitations are considered. After the NURBS blocks have been classified into two cases by the previous module, the velocity scheduling module first calculates the actual maximum feed rate. It then generates the feed rate profiles of all NURBS blocks according to the proposed velocity profile. Later, the round-off error elimination module is applied to adjust the actual maximum feed rate so that the total interpolation time becomes an integer multiple of the interpolation period, which leads to the elimination of round-off errors. Finally, benchmarks are conducted to verify the applicability of the proposed method. Compared with the traditional method, the proposed method can save the interpolation time by $4.67$ to $14.26\% $.

Long-chain bases from Cucumaria frondosa inhibit adipogenesis and regulate lipid metabolism in 3T3-L1 adipocytes

Yingying Tian,Shiwei Hu,Hui Xu,Jingfeng Wang,Changhu Xue,Yuming Wang 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.6

This study aims to investigate anti-adipogenic effects of long-chain bases from Cucumaria frondosa (Cf-LCBs) in vitro. Results showed that Cf-LCBs inhibited adipocyte differentiation and the expressions of CCAAT/enhancer binding proteins (C/EBPs) and peroxisome proliferators-activated receptor γ (PPARγ). Cf-LCBs increased β-catenin mRNA and nuclear translocation and increased its target genes, cyclin D1 and c-myc. Cf-LCBs enhanced fizzled and lipoprotein-receptor-related protein5/6 (LRP5/6) expressions, whereas wingless-type MMTV integration site10b (WNT10b) and glycogen syntheses kinase 3β (GSK3β) remained unchanged. Cf-LCBs also reduced adipogenesis and recovered WNT/β-catenin signaling in the cells suffering from 21H7, a β-catenin inhibitor. In addition, Cf-LCBs decreased triglyceride content and the expressions of lipogenesis genes. Cf-LCBs increased FFA levels and the expressions of lipidolytic factors. Cf-LCBs promoted the phosphorylation of adenosinemonophosphate- activated protein kinase (AMPK) and acetyl-CoA carboxylase. These findings indicate that Cf-LCBs inhibit adipogenesis through activation of WNT/β-catenin signaling and regulate lipid metabolism via activation of AMPK pathway.

Long-chain bases from Cucumaria frondosa inhibit adipogenesis and regulate lipid metabolism in 3T3-L1 adipocytes

Tian, Yingying,Hu, Shiwei,Xu, Hui,Wang, Jingfeng,Xue, Changhu,Wang, Yuming 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.6

This study aims to investigate anti-adipogenic effects of long-chain bases from Cucumaria frondosa (Cf-LCBs) in vitro. Results showed that Cf-LCBs inhibited adipocyte differentiation and the expressions of CCAAT/enhancer binding proteins (C/EBPs) and peroxisome proliferators-activated receptor ${\gamma}(PPAR{\gamma})$. Cf-LCBs increased ${\beta}-catenin$ mRNA and nuclear translocation and increased its target genes, cyclin D1 and c-myc. Cf-LCBs enhanced fizzled and lipoprotein-receptor-related protein5/6 (LRP5/6) expressions, whereas wingless-type MMTV integration site10b (WNT10b) and glycogen syntheses kinase $3{\beta}(GSK3{\beta})$ remained unchanged. Cf-LCBs also reduced adipogenesis and recovered WNT/${\beta}-catenin$ signaling in the cells suffering from 21H7, a ${\beta}-catenin$ inhibitor. In addition, Cf-LCBs decreased triglyceride content and the expressions of lipogenesis genes. Cf-LCBs increased FFA levels and the expressions of lipidolytic factors. Cf-LCBs promoted the phosphorylation of adenosinemonophosphate-activated protein kinase (AMPK) and acetyl-CoA carboxylase. These findings indicate that Cf-LCBs inhibit adipogenesis through activation of WNT/${\beta}-catenin$ signaling and regulate lipid metabolism via activation of AMPK pathway.

Fucoidan from Acaudina molpadioides Protects Pancreatic Islet against Cell Apoptosis via Inhibition of Inflammation in Type 2 Diabetic Mice

Jinhui Wang,Shiwei Hu,Jingfeng Wang,Shijie Li,Wei Jiang 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.1

Inflammation induces pancreatic islet cell apoptosis. Effects of fucoidan from Acaudina molpadioides (Am-FUC) on inhibition of pancreatic islet cell apoptosis and inflammation in type 2 diabetic mice were investigated. Am-FUC repaired pancreatic islet cells, decreased serum C-reactive protein (CRP), macrophage inflammatory protein 1 (MIP-1), interleukin 1β (IL-1β), IL-6, and tumor necrosis factor-α (TNF-α) levels, and increased the IL-10 level. Am-FUC also reduced TNF-α, CRP, MIP-1, IL-1β, and IL-6 mRNA expressions, and increased IL-10 mRNA expression in epididymal adipose tissues. Am-FUC reduced Bid, Bax, cytochrome c, caspase 9, and caspase 3 mRNA expressions, and increased Bcl-2 and Bcl-xL mRNA expressions. Am-FUC down-regulated t-Bid, Bax, cytochrome c, and caspase 9 activities, cleaved caspase 3 proteins, and up-regulated Bcl-2 and Bcl-xL proteins. Thus, an Am-FUCblocked mitochondrial pathway was the suppression mechanism in pancreatic islet cell apoptosis via regulation of inflammatory cytokines providing dietary intervention in type 2 diabetes and inflammation-induced pancreatic islet apoptosis.

A post-genome-wide association study validating the association of the glycophorin C gene with serum hemoglobin level in pig

Yang Liu,Zhengzheng Hu,Chen Yang,Shiwei Wang,Wenwen Wang,Qin Zhang 아세아·태평양축산학회 2017 Animal Bioscience Vol.30 No.5

Objective: This study aimed to validate the statistical evidence from the genome-wide association study (GWAS) as true-positive and to better understand the effects of the glycophorin C (GYPC) gene on serum hemoglobin traits. Methods: Our initial GWAS revealed the presence of two single nucleotide polymorphisms (SNPs) (ASGA0069038 and ALGA0084612) for the hemoglobin concentration trait (HGB) in the 2.48 Mb region of SSC15. From this target region, GYPC was selected as a promising gene that associated with serum HGB traits in pigs. SNPs within the GYPC gene were detected by sequencing. Thereafter, we performed association analysis of the variant with the serum hemoglobin level in three pig populations. Results: We identified one SNP (g.29625094 T>C) in exon 3 of the GYPC gene. Statistical analysis showed a significant association of the SNP with the serum hemoglobin level on day 20 (p<0.05). By quantitative real-time polymerase chain reaction, the GYPC gene was expressed in eight different tissues. Conclusion: These results might improve our understanding of GYPC function and provide evidence for its association with serum hemoglobin traits in the pig. These results also indicate that the GYPC gene might serve as a useful marker in pig breeding programs.

Fucosylated Chondroitin Sulfate from Sea Cucumber Inhibited Islets of Langerhans Apoptosis via Inactivation of the Mitochondrial Pathway in Insulin Resistant Mice

Wei Jiang,Shijie Li,Shiwei Hu,Jignfeng Wang,Wendong Song,Lili Ji,Yaning Wang 한국식품과학회 2015 Food Science and Biotechnology Vol.24 No.3

The effects of fucosylated chondroitin sulphate from sea cucumber (CHS) on inhibition of pancreatic islet apoptosis in high-fat high-sucrose diet (HFSD)-induced insulin resistant mice were studied for 19 weeks. CHS treatment significantly (p<0.05) decreased fasting blood glucose, insulin, and tumor necrosis factor (TNF)-α levels, and increased the serum adiponectin level, improved insulin resistance, and repaired HFSD-injured islets of langerhans, compared with controls. CHS supplementation significantly (p<0.05) inhibited pancreas cell apoptosis via reduction of cytochrome c, caspase 9, and caspase 3 mRNA expressions, cytochrome c release into the cytoplasm, and caspase 9 and cleaved-caspase 3 protein expressions, compared with controls. These effects were associated with upregulation of Bcl-2 and Bcl-xL mRNA and protein expressions, and downregulation of Bax and t-Bid mRNA and protein expressions. Combination treatments with CHS and rosiglitazone synergistically protected pancreatic islets. CHS improves insulin resistance and protects islets of langerhans from apoptosis via inactivation of the intrinsic mitochondrial pathway.

The Effects of Fucosylated Chondroitin Sulfate Isolated from Isostichopus badionotus on Antimetastatic Activity via Downregulation of Hif-1α and Hpa

Min He,Jingfeng Wang,Shiwei Hu,Yuming Wang,Changhu Xue,Hui Li 한국식품과학회 2014 Food Science and Biotechnology Vol.23 No.5

Fucosylated chondroitin sulfate (CHS) wasprepared from the sea cucumber Isostichopus badionotus(Ib-CHS), and the antimetastatic effect was studied usingcell adhesion, migration, invasion, chorioallntoic membrane(CAM), and reverse transcriptase-polymerase chain reaction(RT-PCR) assays. Ib-CHS remarkably suppressed proliferationof 95D cells, and inhibited adhesion, migration, and invasionof 95D cells in a dose-dependent manner. CAM assayresults suggested that Ib-CHS attenuates neovascularizationin the chick embryo. RT-PCR analyses revealed that Ib-CHS dramatically decreased expression of matrix metalloproteinase-2/9 (MMP-2/9), hypoxia-inducible factor (Hif-1α), heparanase (Hpa), and vascular endothelial growthfactor (VEGF), and increased expression levels of the tissueinhibitors of metalloproteinase-1/2 (TIMP-1/2), importantinhibitors of MMP-2/9. Ib-CHS markedly inhibited tumorgrowth, improved the metastatic foci occurrence rate, anddown-regulated expressions of Hif-1α, Hpa, and VEGF invivo. Ib-CHS exerted antimetastatic activities in vitro andin vivo.