Cisplatin과 Tobramycin이 白鼠 Corti器에 미치는 영향에 關한 電子 顯微鏡的 硏究 : Ultrastructural Study

김동환,신태균,김무강,김홍선,김원식 충남대학교 의과대학 지역사회의학연구소 1989 충남의대잡지 Vol.16 No.1

In order to study the morphological changes of Corti’s organ in the rat treated with cisplatin and tobramycin, thirty-three rats, weighing between 200∼250g, were used, and divided into 4 groups, i.e., 3 rats were used for control, and 30 rats were used for 3 experimental groups. Each experimental group was divided into cisplatin-treated, tobramycin-treated, and both cisplatin and tobramycin treated group with 10 rats. Rats were injected with a single dose of 7.7㎎/㎏ of cisplatin intraperitoneally and 50㎎/㎏/day of tobramycin for 2 days intramuscularly, respectively or both. Treated rats were sacrificed on the 3rd and 5th day after the first injection. Results were as follows; 1. Cisplatin-treated group: Outer hair cells of Corti’s organ were degenerated with the formation of vacuoles and appearance of lysosomes, however, inner hair cells showed mild vacuolization. 2. Tobramycin-treated group: NO pathological changes were found in Corti’s organ. 3. Combined cisplatin and tobramycin treated group: Outer and inner hair cells showed vacuolization, appearance of lysosomes, myelin figures and multivesicular body. There were also multivesicular body in the inner phalangeal cell and myelin figure in the Reissner’s membrance. 4. Degeneration of Corti’s organ in the combined treated group was more sever than those of the single cisplatin-treated or tobramycin-treated group. 5. NO pathological differences of Corti’s organ in duration of postinjection were manifested in any groups. It can be concluded that cisplatin induces toxicity to inner ear and cisplatin enhance toxicity more in continued treated with tobramycin.

Vibrio vulnificus에 의한 패혈증 3예

김신무,김유현,신상희,오재세,강숙희,윤여안,이화재,김영옥 대한임상병리사협회 1986 임상병리사회지 Vol.18 No.1

국가예방접종전산등록체계 개발을 위한 민간의료 기관 하부구조 및 환경 조사연구

이무식,김은영,이건세,이석구,홍지영,김건엽,김대경,신의철,이연경,기몽서,나백주,김영택 한국모자보건학회 2003 한국모자보건학회지 Vol.7 No.2

각종 동물 설유두의 형태학적인 관찰

김홍선,김무강,김원식,이영호,신태균 충남대학교 의과대학 지역사회의학연구소 1987 충남의대잡지 Vol.14 No.2

The lingual papillae of the mouse, the rabbit, and the cat were observed by light microscope and scanning electron microscope The results were followings 1. The filiform papillae of the mouse were classified to typical filiform papillae, conical filiform papillae with 2 branched tips, and general conical filiform papillae. The outmost layers of the filiform papillae of the mouse keratinted well, middle layer of the anterior portion of the papillae appeaered a lot of small basophilic granules. The fungiform papillae existed between the filiform papillae with the peripheral folds of epithelia. A vallate papilla existed on the posterior portion of conical filiform papillae and their shape was lenticuiar. 2. The filiform papillae of the rabbit revealed various type (5 types). Keratin layer of the papillae existed vertical to long axis of the papillae on the posterior portion of the papillae. Stratified squamous epithelium on the anterior portion of the papillae existed with a few keratin layers and the intercellular materials of strong PAS positive reaction appeared between the these cells. The fungiform papillae of the rabbit existed between the filiform papillae and had a few peripheral folds. The foliate papillae of the rabbit existed on the lateral portion of tongue root, consisted of the paralleled folds. Taste buds located in the mid-lateral portion of the papillae concentrically. 3. The filiform papillae on the anterior portion of the cat tongue shaped anterior and posterior projection, the filiform palillae on the middle portion had a projections of columnar shape, which papillae surface appeared as onion surface. The conical filiform papillae on the posterior portion had many folds on the lower portion of the papillae. The fungiform papillae of the cat existed between the filiforr papillae with a columnar shaped projectionand proturded from the surface. The vallate papillae of the cat existed between the conical filiform papillae and their shape was typical form of vallate papillae.

대학생들의 학교생활 적응에 관한 연구

신은숙,김현덕,민무숙 順天鄕大學校 學生生活硏究所 1995 學生生活硏究 Vol.2 No.-

This study examines college students' adjustment to their campus lives. The hypothesis of this research is that the school experiences of college students are different depending on the background of those students, and these influence their adjustment to their lives in college. The study sample included 293 students who are freshmen, sophomore, and junior students at Soonchunhyang University in Onyang City. First, the school experiences were measured with respect to six background variables from two points of view: the extents of their use of school facilities and hours of staying on campus. School adjustment score were also measured depending on the six background variables of students. Four areas of adjustment were also attained: academic adjustment, Social adjustment, personl_emotional adjustment, and attachment. These four facets were analyzed with relation to the school experiences. Statistical analysis showed that the level of school adjustment were different depending on three background variables, such as gender, economic condition, and commuting distance. That is, the male students showed higher degree of school adjustment than the females; the students with poor economic condition showed the lowest adjustment score; those who are living in the university dormitory were proved to be generally competent in adjusting themselves in campus life. Furthermore, the subscores of school adjustment were different depending on all of six background variabls. The statistical analysis also showed that there existed no relationship between the adjustment level and the frequencies of the school facilities. However, the hours of staying on campus showed a positive relationship with the adjustment. This was variant depending on gender and commuting distance. That is, the male students stayed longer on campus and showed higher adjustment, scores than the female students; the students living in the university dormitory stayed longer in campus and showed higher adjustment scores than the commuting students from long distance, such as Seoul, and Taejeon.

鷄胎眼球成長에 미치는 Mitomycin C의 影響에 關한 硏究

申潤澈,金武剛 충남대학교 의과대학 지역사회의학연구소 1984 충남의대잡지 Vol.11 No.1

In order to know about the effect of the mitomycin C on the eye ball growth in developing chick embryo, author injected mitomycin 8ug or 16 ug/2m ℓ/egg into the chorioallantoic membrane of 1, 000 eggs on the 6th day after initial day of the incubation. After then selected the 70 eggs per day among the incubated eggs at random from the 12th day to the 18th day with 48 hour intervals and brocked the incubated eggs in order to get the chick eye balls. The removed chick embryo eye balls were measured the weights with the Torsion balance and calculated the arithmetic mean, standard deviation, coefficient of variability, and the significant difference by the anayslis of variance. After then, analyzed the growth factor, that is, the theoretical growth quantity, growth rate, specific growth rate and the ratio of the growth quantity by the growth formula utilizing the arithmetic mean of the actually measured eye ball weigfits, and the coefficients of relative growth rates of the mitomycin C injected groups to the control group on the developing chick eye balls by the relative growth formula. The results obtained were summarized as follows; 1. Significant differences between the mean of the control group and injected groups, and between the 8 ug and 16ug injected groups were also significant at the 5 per cent level on the 12th day, and at the 0. 5 per cent level from the 14th day to the 18th day.. 2. The differences between the arithmetic means of the actually measured eye balls weights and growth quantities calculated by the growth formula were below the 0.352% error. 3. Growth rates were decreased in the control group and 16,ug injected group which was severe compare to the control group, and increased in the 8 ug injected group. 4. Specific growth rates were decreased in all three groups and the decrease rates were severe in order, control, 16u.g and 8ug. 5. Growth gradients of injected groups were larger compare to control group, and growth centers of injected groups were in the 18th day. 6. Relative growth rates of the injected groups were positive allometry (a>l) compare to the control group and the 16ug injected group was more positive allometry than 8ug injected group.

Mongolian Gerbil과 CJU/A Mouse의 와우에서 βNADPH Diaphorase의 발현 양상

추무진,김진섭,송창근,신시옥,진홍률 대한이비인후과학회 1999 대한이비인후과학회지 두경부외과학 Vol.42 No.11

생쥐 위장관의 Interstitial cells of Cajal(ICC)에 대한 면역조직화학적 연구

김영철,차경훈,신무경,임건한,김주영,안병수,김장만,양경철,박도영,오재욱,장인엽 조선대학교 2003 The Medical Journal of Chosun University Vol.28 No.1

Background and Objectives : Interstitial cells of Cajal(ICC) are the pacemakers in gastrointestinal tract that modulates gastrointestinal motiliey and these cells also transmit neural input from enteric nerves to smooth muscles. Recent work on tissues from patients with motility disorders that suggest that loss or defect in ICC could be related to pathophysiology in human and animal models. Immunolabelling of ICC in intestinal wall is recently developed by using specific marker, anti-c-kit antibody. Immunohistochemistry was done for ICC network in attempt to provide a morphological basis for the mechanism regulating gastrointestinal motility Methods : Cryosection was done, and whole-mount preparations of mouse stomach, gastrointestinal tract were immunolabelled using the anti-c-Kit. Immunolabelled ICC networks were observed under a confocal laser scanning microscopy. Results : According to three dimensional reconstruction study, we found that the c-Kit-positive celluar networks were widely distributed in the gastrointestinal muscle (1) circular muscle layer(IC-IM), (2) myenteric plexus(IC-MY), (3) deep muscular plexus(IC-DMP) in ileum, (4) submucosal plexus(IC-SMP) and longitudinal muscle layer(IC-LM) in colon. Conclusion : The characteristic profiles of ICC celluar networks provide a morphological basis upon the mechanism regulating gastrointestinal motility. Additional studies for the enteric nerves-ICC interaction are need to evaluate the detailed roles of Icc in gastrointestinal tract.

심근침범을 동반한 Becker형 근이영양증 환자에서 확인한 Dystrophin 유전자 Exon결실

김광일,오병희,이무용,채인호,신수,박성섭,김효수,손대원,이명묵,박영배,최윤식,이영우 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.5

직장암 수술 후 국소재발과 예후

김태윤,백무준,김성용,신응진,박내경,이문수,김창호,송옥평 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.1

Locally recurrent rectal cancer is a difficult clinical problem. Local recurrence following treatment for rectal cancer is a major cause of morbidity and mortality. The reported range of local recurrence rates following surgical treatment for rectal cancer has varied from 4% to 50%. We retrospectively reviewed the charts of 142 rectal cancer patients from January 1991 to December 1999. Analysis of patients factors included age, gender, type of surgery, and adjuvant therapy. Tumors were assessed for level, stage, and grade. Local recurrence and distant metastases were also documented. Variables influencing local recurrence in this group were identified and disease-free survival is determined. Of the 142 patients(74 males, 68 females; mean age 62.6 years), 137 consecutive patients operated on for rectal cancer and followed up to December 2000 (range 23-118months) were analyzed. Local recurrence rate was 18.3%. Age and sex of patients, type of surgery, location of tumor in the rectum, size, morphology and grading of the tumor were all unrelated to the event under investigation. At Cox regression, the Dukes stage and the postoperative radiotherapy were the only independent prognostic factors for local recurrence, and Dukes stage (Hazard ratio=2.89, p<0.001) and local recurrence (Hazard ratio=3.31, p<0.01) were the only independent factors associated with improved survival.