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Seunghyup Jeong,Unyong Kim,Hyun Joo An 한국당과학회 2018 한국당과학회 학술대회 Vol.2018 No.01
Gastric cancer is one of the most common malignancy and leading cause of cancer death. Classically, CEA and CA19-9 have been used for gastric cancer detection. However, CEA and CA19-9 insufficient for cancer detection due to low specificity and sensitivity. Glycosylation is the most common post-translational modification and plays an important role in various biological processes. Whole serum based glycan profiling has been already developed and widely used in cancer biomarker study. While, targeted glycoproteomic approach is needed in clinic for better sensitivity and specificity. In previous study, we targeted serum haptoglobin for gastric cancer. We found glycosylation changes of haptoglobin on released N-glycan between gastric cancer patient and healthy control. Released glycan analysis provide compositional and isomer information while cannot give actual site of glycosylation. On the other hand, intact glycopeptide analysis provide site-specific information. Based on this actual glycosylation site and glycan heterogeneity, we monitored more detailed glycan changes and aberrant glycosylation of each site for more sensitive and specific diagnosis. In this study, we have developed the method for intact glycopeptide analysis for biomarker discovery using UHPLC MS. We successfully separated 3 glycopeptides and profiled the glycoform of 2 glycopeptides. The other one was difficult to profile glycoform according to two glycosylation sites. Therefore, this glycopeptide was fractionated and analyzed by nano LC chip Q-TOF MS. Further, cancer patient (n=40, stage IV) and healthy control (n=47) samples were applied for training set of biomarker study. A T-test based analysis was performed to identify potential biomarker (p<0.001). In the future, we will apply this platform to large clinical sample to validate potential biomarker.
Seunghyup Jeong,Serenus Hua,Do-Young Choi,Pyong-Gon Moon,Rudolf Grimm,Kwang Pyo Kim,Moon Chang Baek,Hyun Joo An 한국당과학회 2013 한국당과학회 학술대회 Vol.2013 No.1
Exosomes (microvesicles, or micropaticles) are small membrane-enclosed vesicles and these are secreted by various cell types, including tumor cells. These vesicles play an important role as mediators in extracellular communication. They are composed of membrane, cellular proteins, DNA, and RNA derived from their origin cells. It is also known that exosomes are involved in tumor metastasis, angiogenesis, and antitumor immunity. These biological functions are probably due to the glycosylation on their membrane proteins. Thus, the study of glycosylation of exosomes will be another potential source of new biomarker. However, there is a little study about the glycosylation of exosomes. Here, we targeted and analyzed N-glycans of exosomes derived from five cancer cell lines (A549, PC9, PC9/ZD, MCF-7, and MDA-MB231) using nano-LC/MS. We also have compared glycans of exosomes with glycans on their origin cell membrane to examine glycosylation correlation between origin cells and exosomes on three lung cancer cell lines. Additionally, we have compared anticancer drug resistant cell line PC9/ZD (Gefitinib resistant) and untreated PC9 in terms of origin cells and exosomes glycan profiling via isomer separation. Exosomes and cell membranes were prepared from three lung cancer cell lines: A549, PC9, and PC9/ZD. And two breast cancer exosomes were prepared: MCF-7 and MDA-MB231. Glycans were released by PNGase F, then enriched by graphitized carbon solid phase extraction. Nano-LC/chip Q-TOF MS was used for overall glycan profiling and quantitation. We successfully release and profile N-glycans from exosomes. Origin cells and exosomes of lung cancer contain high-mannose glycans in abundance. Although, exosomes have less high-mannose glycans compared with origin cells. Both origin cells and exosomes, isomer separation of sialylated glycans are different between PC9 and PC9/ZD. On breast cancer exosomes, two different matastatic samples represent quite different profiling. This is the first study of comprehensive glycan profiling of exosomes using mass spectrometry.
Spontaneous Additive Nanopatterning from Solution Route Using Selective Wetting
Jeong, Hyeonho,Moon, Hanul,Kim, Han-Jung,Yoon, Min,Park, Chang-Goo,Oh, Yong Suk,Sung, Hyung Jin,Choi, Dae-Geun,Yoo, Seunghyup American Chemical Society 2018 ACS APPLIED MATERIALS & INTERFACES Vol.10 No.31
<P>Nanopatterns of functional materials have successfully led innovations in a wide range of fields, but further exploration of their full potential has often been limited because of complex and cost-inefficient patterning processes. We here propose an additive nanopatterning process of functional materials from solution route using selective wetting phenomenon. The proposed process can produce nanopatterns as narrow as 150 nm with high yield over large area at ultrahigh process speed, that is, the speed of solution dragging, of up to ca. 4.6 m·min<SUP>-1</SUP>. The process is highly versatile that it can utilize a wide range of solution materials, control vertical structures including pattern thickness and multistacks, and produce nanopatterns on various substrates with emerging form factors such as foldability and disposability. The solution patterning in nanoscale by selective wetting is enabled by corresponding surface energy patterns in high contrast that are achieved by one-step imprinting onto hydrophobic/hydrophilic bilayers. The mechanisms and control parameters for the solution patterning are revealed by fluid-dynamic simulation. With the aforementioned advantages, we demonstrate 25 400 pixel-per-inch light-emitting pixel arrays and a plasmonic color filter of 10 cm × 10 cm area on a plastic substrate as potential applications.</P> [FIG OMISSION]</BR>
Jeong, Su-Hun,Woo, Seong-Hoon,Han, Tae-Hee,Park, Min-Ho,Cho, Himchan,Kim, Young-Hoon,Cho, Hyunsu,Kim, Hobeom,Yoo, Seunghyup,Lee, Tae-Woo Nature Publishing Group 2017 NPG Asia Materials Vol.9 No.7
<P>Flexible transparent electrode materials such as conducting polymers, silver nanowires, carbon nanotubes and graphenes are being investigated as possible replacements for conventional brittle inorganic electrodes. However, they have critical drawbacks of low work function (WF), resulting in a high hole injection barrier to an overlying semiconducting layer in simplified organic or organic-inorganic hybrid perovskite light-emitting diodes (OLEDs or PeLEDs). Here, we report a new anode material (AnoHIL) that has multifunction of both an anode and a hole injection layer (HIL) as a single layer. The AnoHIL has easy WF tunability up to 5.8 eV and thus makes ohmic contact without any HIL. We applied our anodes to simplified OLEDs, resulting in very high efficiency (62% ph el(-1) for single and 88% ph el(-1) for tandem). The AnoHIL showed a similar tendency in simplified PeLEDs, implying universal applicability to various optoelectronics. We also demonstrated large-area flexible lightings using our anodes. Our results provide a significant step toward the next generation of high-performance simplified indium tin oxide (ITO)-free light-emitting diodes.</P>
Overcoming the electroluminescence efficiency limitations of perovskite light-emitting diodes
Cho, Himchan,Jeong, Su-Hun,Park, Min-Ho,Kim, Young-Hoon,Wolf, Christoph,Lee, Chang-Lyoul,Heo, Jin Hyuck,Sadhanala, Aditya,Myoung, NoSoung,Yoo, Seunghyup,Im, Sang Hyuk,Friend, Richard H.,Lee, Tae-Woo American Association for the Advancement of Scienc 2015 Science Vol.350 No.6265
<P><B>Brighter perovskite LEDs</B></P><P>Organic-inorganic hybrid perovskites such as methyl ammonium lead halides are attractive as low-cost light-emitting diode (LED) emitters. This is because, unlike many inorganic nanomaterials, they have very high color purity. Cho <I>et al.</I> made two modifications to address the main drawback of these materials, their low luminescent efficiency. They created nanograin materials lacking free metallic lead, which helped to confine excitons and avoid their quenching. The perovskite LEDs had a current efficiency similar to that of phosphorescent organic LEDs.</P><P><I>Science</I>, this issue p. 1222</P><P>Organic-inorganic hybrid perovskites are emerging low-cost emitters with very high color purity, but their low luminescent efficiency is a critical drawback. We boosted the current efficiency (CE) of perovskite light-emitting diodes with a simple bilayer structure to 42.9 candela per ampere, similar to the CE of phosphorescent organic light-emitting diodes, with two modifications: We prevented the formation of metallic lead (Pb) atoms that cause strong exciton quenching through a small increase in methylammonium bromide (MABr) molar proportion, and we spatially confined the exciton in uniform MAPbBr<SUB>3</SUB> nanograins (average diameter = 99.7 nanometers) formed by a nanocrystal pinning process and concomitant reduction of exciton diffusion length to 67 nanometers. These changes caused substantial increases in steady-state photoluminescence intensity and efficiency of MAPbBr<SUB>3</SUB> nanograin layers.</P>